Restriction map for
pFN21AE4029
(Circular) (Six-base) MAPSORT of: pFN21AE4029.seq Check: 185 from: 1 to: 5413
pFN21AE4029 5413 bp
With 182 enzymes: SgfI *
March 28, 2012 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2168 2168
Size: 5413
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2169 2734 2169
Size: 565 4848
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2317 3476 4860 5128 1632
Size: 685 1159 1384 268 1917
Fragments arranged by size:
1917 1384 1159 685 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 4142 4142
Size: 5413
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5413
AlwNI CAG_nnn'CTG
Cuts at: 2524 3156 4728 2524
Size: 632 1572 3209
Fragments arranged by size:
3209 1572 632
ApaI G_GGCC'C
Cuts at: 2053 2498 2053
Size: 445 4968
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2722 2948 3100 5209 1493
Size: 1229 226 152 2109 1697
Fragments arranged by size:
2109 1697 1229 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2384 2738 1870
Size: 73 441 354 4545
Fragments arranged by size:
4545 441 354 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2301 2334 2301
Size: 33 5380
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2169 2410 2429 2734
Size: 325 217 627 336 46 241 19 305
Cuts at: 2734 4189 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 336 325 305 241 217
46 19
BanII G_rGCy'C
Cuts at: 1391 2053 2236 2253 2498 2732 1391
Size: 662 183 17 245 234 4072
Fragments arranged by size:
4072 662 245 234 183 17
BbeI G_GCGC'C
Cuts at: 2127 2414 2127
Size: 287 5126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 928
Size: 971 412 4030
Fragments arranged by size:
4030 971 412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2128 2162 2529 2563 3604
Size: 34 50 34 107 34 367 34 1041
Cuts at: 3604 3638 1903
Size: 34 3678
Fragments arranged by size:
3678 1041 367 107 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2302 3305 4515 2302
Size: 1003 1210 3200
Fragments arranged by size:
3200 1210 1003
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2493 4024 136
Size: 107 122 71 631 605 821 1531 1525
Fragments arranged by size:
1531 1525 821 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5408 5408
Size: 5413
BlpI GC'TnA_GC
Cuts at: 2021 2563 2830 2021
Size: 542 267 4604
Fragments arranged by size:
4604 542 267
Bme1580I G_kGCm'C
Cuts at: 1790 2053 2432 2498 2505 3474 4630 1790
Size: 263 379 66 7 969 1156 2573
Fragments arranged by size:
2573 1156 969 379 263 66 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4102 442
Size: 416 53 83 399 24 1727 958 1753
Fragments arranged by size:
1753 1727 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5413
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 4073 1239
Size: 1421 1413 2579
Fragments arranged by size:
2579 1421 1413
Bpu10I CC'TnA_GC
Cuts at: 2517 2517
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2576 3538 4403 4701 2576
Size: 962 865 298 3288
Fragments arranged by size:
3288 962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2411 3220 3602
Size: 53 83 186 936 591 287 809 382
Cuts at: 3602 4237 275
Size: 635 1451
Fragments arranged by size:
1451 936 809 635 591 382 287 186
83 53
BsaWI w'CCGG_w
Cuts at: 2236 3846 4244 4518 4665 5076 2236
Size: 1610 398 274 147 411 2573
Fragments arranged by size:
2573 1610 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 1178
Size: 30 165 30 820 30 4338
Fragments arranged by size:
4338 820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5413
BseYI C'CCAG_C
Cuts at: 2092 2348 4616 5112 2092
Size: 256 2268 496 2393
Fragments arranged by size:
2393 2268 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 1163
Size: 573 627 4213
Fragments arranged by size:
4213 627 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2236 2253 2564 2628 3474 3559 4630 2236
Size: 17 311 64 846 85 1071 3019
Fragments arranged by size:
3019 1071 846 311 85 64 17
BsmI GAATG_Cn'
Cuts at: 2659 2924 3017 2659
Size: 265 93 5055
Fragments arranged by size:
5055 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2236 2253 2432 2498 2505 2564
Size: 399 263 183 17 179 66 7 59
Cuts at: 2564 2628 2732 3474 3559 4630 1391
Size: 64 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 263 183 179 104
85 66 64 59 17 7
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2168 2768 844
Size: 1324 600 3489
Fragments arranged by size:
3489 1324 600
BsrBI CCG'CTC
Cuts at: 1394 1823 2044 3300 1394
Size: 429 221 1256 3507
Fragments arranged by size:
3507 1256 429 221
BsrDI GCAATG_nn'
Cuts at: 65 1248 2170 3908 4082 65
Size: 1183 922 1738 174 1396
Fragments arranged by size:
1738 1396 1183 922 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 4057 4244 1700
Size: 110 374 1873 187 2869
Fragments arranged by size:
2869 1873 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 2079 2177 1925
Size: 154 98 5161
Fragments arranged by size:
5161 154 98
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2103 794
Size: 1309 4104
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2388 2535 2743 3110 3495 3512 4964 5050
Size: 416 147 208 367 385 17 1452 86
Cuts at: 5050 5062 5408 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 416 385 367 346 208 147
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2444 2444
Size: 5413
BtgI C'CryG_G
Cuts at: 513 1065 2012 2066 2127 513
Size: 552 947 54 61 3799
Fragments arranged by size:
3799 947 552 61 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2190 2929 3723 3743 2190
Size: 739 794 20 3860
Fragments arranged by size:
3860 794 739 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 2860 3749
Size: 54 1425 50 275 18 696 334 889
Cuts at: 3749 5378 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 696 334 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3343 1353
Size: 1990 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2234 2251 2234
Size: 17 5396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2317 2660 3476 4073 4860 5128 1239
Size: 393 685 343 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 685 597 393 343 268
EcoO109I rG'GnC_Cy
Cuts at: 2050 2494 2495 2050
Size: 444 1 4968
Fragments arranged by size:
4968 444 1
EcoRI G'AATT_C
Cuts at: 2722 3100 2722
Size: 378 5035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5413
FspI TGC'GCA
Cuts at: 2243 2669 3919 2243
Size: 426 1250 3737
Fragments arranged by size:
3737 1250 426
FspAI rTGC'GCAy
Cuts at: 2243 2669 2243
Size: 426 4987
HaeII r_GCGC'y
Cuts at: 1678 2127 2414 4560 1678
Size: 449 287 2146 2531
Fragments arranged by size:
2531 2146 449 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 4054 4086 4128
Size: 32 135 32 620 32 1830 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 620 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 2757 3003 669
Size: 988 281 520 299 246 3079
Fragments arranged by size:
3079 988 520 299 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5413
HpaI GTT'AAC
Cuts at: 3003 3003
Size: 5413
KasI G'GCGC_C
Cuts at: 2123 2410 2123
Size: 287 5126
KpnI G_GTAC'C
Cuts at: 2173 2738 2173
Size: 565 4848
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2068 2264 2473 2682 2688 2829 3506
Size: 408 54 196 209 209 6 141 677
Cuts at: 3506 4654 4899 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 408 245 209 209 196
141 54 6
NarI GG'CG_CC
Cuts at: 2124 2411 2124
Size: 287 5126
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4861
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5413
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2771 4316 1132
Size: 97 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5413
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PspOMI G'GGCC_C
Cuts at: 2049 2494 2049
Size: 445 4968
PstI C_TGCA'G
Cuts at: 830 2765 830
Size: 1935 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
PvuII CAG'CTG
Cuts at: 2473 2473
Size: 5413
SacI G_AGCT'C
Cuts at: 2236 2253 2236
Size: 17 5396
SacII CC_GC'GG
Cuts at: 2015 2069 2015
Size: 54 5359
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2761 3896 4577 4768 826
Size: 219 1716 1135 681 191 1471
Fragments arranged by size:
1716 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2493 2493
Size: 5413
SfoI GGC'GCC
Cuts at: 2125 2412 2125
Size: 287 5126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 3517 4418 4680 820
Size: 197 926 612 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 612 262 197
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2771 1132
Size: 1639 3774
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2852 513
Size: 552 1787 3074
Fragments arranged by size:
3074 1787 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2298 2447 3440 3457 3610 3795 4240
Size: 1255 718 149 993 17 153 185 445
Cuts at: 4240 325
Size: 1498
Fragments arranged by size:
1498 1255 993 718 445 185 153 149
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3659 5371 96
Size: 274 80 33 51 1443 1682 1712 138
Fragments arranged by size:
1712 1682 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 5373 5405 2223
Size: 32 3118 32 2231
Fragments arranged by size:
3118 2231 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2453 1316
Size: 1137 4276
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 2696 2699 3542 2696
Size: 3 843 4567
Fragments arranged by size:
4567 843 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI EcoRV FseI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SanDI SapI SexAI SrfI SwaI XcmI