Restriction map for
pFN21AE4027
(Circular) (Six-base) MAPSORT of: pFN21AE4027.seq Check: 9580 from: 1 to: 5413
pFN21AE4027 5413 bp
With 182 enzymes: SgfI *
March 28, 2012 13:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3223 278
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 1937
Size: 819 4594
Acc65I G'GTAC_C
Cuts at: 2734 2734
Size: 5413
AclI AA'CG_TT
Cuts at: 1563 3540 3913 1563
Size: 1977 373 3063
Fragments arranged by size:
3063 1977 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2553 2667 3476 4860 5128 1632
Size: 921 114 809 1384 268 1917
Fragments arranged by size:
1917 1384 921 809 268 114
AfeI AGC'GCT
Cuts at: 2470 2470
Size: 5413
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5216
AflIII A'CryG_T
Cuts at: 1203 4312 5289 1203
Size: 3109 977 1327
Fragments arranged by size:
3109 1327 977
AgeI A'CCGG_T
Cuts at: 4244 4244
Size: 5413
AhdI GACnn_n'nnGTC
Cuts at: 2624 4142 2624
Size: 1518 3895
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5413
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2225 1184
Size: 1041 4372
AlwNI CAG_nnn'CTG
Cuts at: 3156 4728 3156
Size: 1572 3841
ApaLI G'TGCA_C
Cuts at: 3470 4626 3470
Size: 1156 4257
ApoI r'AATT_y
Cuts at: 1493 2409 2722 2948 3100 5209 1493
Size: 916 313 226 152 2109 1697
Fragments arranged by size:
2109 1697 916 313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3967 160
Size: 872 2935 1606
Fragments arranged by size:
2935 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
AvaI C'yCGr_G
Cuts at: 1870 1943 2738 1870
Size: 73 795 4545
Fragments arranged by size:
4545 795 73
BamHI G'GATC_C
Cuts at: 2743 3110 2743
Size: 367 5046
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 4189 618
Size: 325 217 627 947 1455 1842
Fragments arranged by size:
1842 1455 947 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2275 2732 1391
Size: 884 457 4072
Fragments arranged by size:
4072 884 457
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3604 3638 1903
Size: 34 1667 34 3678
Fragments arranged by size:
3678 1667 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2202 3305 4515 2202
Size: 1103 1210 3100
Fragments arranged by size:
3100 1210 1103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2545 4024 136
Size: 107 122 71 631 605 873 1479 1525
Fragments arranged by size:
1525 1479 873 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2458 5408 2458
Size: 2950 2463
BlpI GC'TnA_GC
Cuts at: 2830 2830
Size: 5413
Bme1580I G_kGCm'C
Cuts at: 1790 3474 4630 1790
Size: 1684 1156 2573
Fragments arranged by size:
2573 1684 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3144 4102 442
Size: 416 53 83 399 24 1727 958 1753
Fragments arranged by size:
1753 1727 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2144 1056
Size: 1088 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4073 1239
Size: 2834 2579
Bpu10I CC'TnA_GC
Cuts at: 2543 2543
Size: 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2462 3538 4403 4701 2285
Size: 177 1076 865 298 2997
Fragments arranged by size:
2997 1076 865 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 882
Size: 617 2577 2219
Fragments arranged by size:
2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3204 493
Size: 711 2000 2702
Fragments arranged by size:
2702 2000 711
BsaBI GATnn'nnATC
Cuts at: 5407 5407
Size: 5413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2548 3220 3602 4237
Size: 53 83 186 936 1015 672 382 635
Cuts at: 4237 275
Size: 1451
Fragments arranged by size:
1451 1015 936 672 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3846 4244 4518 4665 5076 3846
Size: 398 274 147 411 4183
Fragments arranged by size:
4183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2262 2292 1178
Size: 30 165 30 859 30 4299
Fragments arranged by size:
4299 859 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 2573 1186
Size: 962 425 4026
Fragments arranged by size:
4026 962 425
BseYI C'CCAG_C
Cuts at: 4616 5112 4616
Size: 496 4917
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2692 1163
Size: 573 956 3884
Fragments arranged by size:
3884 956 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2863 3624 4652 1540
Size: 275 186 862 761 1028 2301
Fragments arranged by size:
2301 1028 862 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2275 3474 3559 4630 2275
Size: 1199 85 1071 3058
Fragments arranged by size:
3058 1199 1071 85
BsmI GAATG_Cn'
Cuts at: 2924 3017 2924
Size: 93 5320
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2732 3474 3559 4630 1391
Size: 399 485 457 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 457 399 85
BspEI T'CCGG_A
Cuts at: 5076 5076
Size: 5413
BspHI T'CATG_A
Cuts at: 3302 5032 3302
Size: 1730 3683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 844
Size: 1924 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3300 1394
Size: 429 1477 3507
Fragments arranged by size:
3507 1477 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2510 3908 4082 65
Size: 1183 1262 1398 174 1396
Fragments arranged by size:
1398 1396 1262 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4057 4244 1700
Size: 110 2247 187 2869
Fragments arranged by size:
2869 2247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5413
BssSI C'ACGA_G
Cuts at: 3473 4485 3473
Size: 1012 4401
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5413
BstBI TT'CG_AA
Cuts at: 4217 4217
Size: 5413
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5413
BstYI r'GATC_y
Cuts at: 1972 2164 2458 2743 3110 3495 3512 4964 5050
Size: 192 294 285 367 385 17 1452 86
Cuts at: 5050 5062 5408 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 294 285 192
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4861
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4674
Fragments arranged by size:
4674 683 56
BtsI GCAGTG_nn'
Cuts at: 2929 3723 3743 2929
Size: 794 20 4599
Fragments arranged by size:
4599 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5413
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5378
DraI TTT'AAA
Cuts at: 2717 2717
Size: 5413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4420 809
Size: 507 3104 1802
Fragments arranged by size:
3104 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2860 3749 5378
Size: 54 1425 50 275 18 1030 889 1629
Cuts at: 5378 8
Size: 43
Fragments arranged by size:
1629 1425 1030 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2860 1537
Size: 275 1048 4090
Fragments arranged by size:
4090 1048 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2211 2529 3343 1353
Size: 858 318 814 3423
Fragments arranged by size:
3423 858 814 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4000 4374 4520 387
Size: 1064 138 2411 374 146 1280
Fragments arranged by size:
2411 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 5413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2553 2667 3476 4073 4860 5128 1239
Size: 393 921 114 809 597 787 268 1524
Fragments arranged by size:
1524 921 809 787 597 393 268 114
EcoRI G'AATT_C
Cuts at: 2409 2722 3100 2409
Size: 313 378 4722
Fragments arranged by size:
4722 378 313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2087 2087
Size: 5413
FspI TGC'GCA
Cuts at: 3919 3919
Size: 5413
HaeII r_GCGC'y
Cuts at: 1678 2472 4560 1678
Size: 794 2088 2531
Fragments arranged by size:
2531 2088 794
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2610 2642 4054 4086 4128
Size: 32 135 32 1038 32 1412 32 42
Cuts at: 4128 4160 1373
Size: 32 2626
Fragments arranged by size:
2626 1412 1038 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2656 2757 3003 669
Size: 988 281 121 597 101 246 3079
Fragments arranged by size:
3079 988 597 281 246 121 101
HindIII A'AGCT_T
Cuts at: 748 2564 748
Size: 1816 3597
HpaI GTT'AAC
Cuts at: 2059 3003 2059
Size: 944 4469
KpnI G_GTAC'C
Cuts at: 2738 2738
Size: 5413
MfeI C'AATT_G
Cuts at: 3012 3012
Size: 5413
MluI A'CGCG_T
Cuts at: 5289 5289
Size: 5413
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4527 4711 1305
Size: 3222 184 2007
Fragments arranged by size:
3222 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3591
Fragments arranged by size:
3591 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3371 3730 3889 518
Size: 648 2205 359 159 2042
Fragments arranged by size:
2205 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2829 3506 4654 4899 1606
Size: 1223 677 1148 245 2120
Fragments arranged by size:
2120 1223 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4861
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5413
NheI G'CTAG_C
Cuts at: 1052 2140 1052
Size: 1088 4325
NotI GC'GGCC_GC
Cuts at: 2860 2860
Size: 5413
NspI r_CATG'y
Cuts at: 1132 1229 2183 2771 4316 1132
Size: 97 954 588 1545 2229
Fragments arranged by size:
2229 1545 954 588 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5413
PciI A'CATG_T
Cuts at: 4312 4312
Size: 5413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5413
PmeI GTTT'AAAC
Cuts at: 2717 2717
Size: 5413
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 4995 5027 3482
Size: 32 1481 32 3868
Fragments arranged by size:
3868 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2661 1613
Size: 1048 4365
PsiI TTA'TAA
Cuts at: 2983 2983
Size: 5413
PstI C_TGCA'G
Cuts at: 830 2765 830
Size: 1935 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5413
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 5413
SalI G'TCGA_C
Cuts at: 1936 2755 1936
Size: 819 4594
SapI GCTCTTCn'nnn_
Cuts at: 2529 2529
Size: 5413
SbfI CC_TGCA'GG
Cuts at: 2765 2765
Size: 5413
ScaI AGT'ACT
Cuts at: 3661 5373 3661
Size: 1712 3701
SfcI C'TryA_G
Cuts at: 826 1045 2094 2484 2761 3896 4577 4768 826
Size: 219 1049 390 277 1135 681 191 1471
Fragments arranged by size:
1471 1135 1049 681 390 277 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5413
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5413
SmaI CCC'GGG
Cuts at: 1872 2740 1872
Size: 868 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2441 3517 4418 4680 820
Size: 197 926 321 177 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 321 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3204 493
Size: 2711 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5413
SphI G_CATG'C
Cuts at: 1132 2183 2771 1132
Size: 1051 588 3774
Fragments arranged by size:
3774 1051 588
SspI AAT'ATT
Cuts at: 5 52 3337 5
Size: 47 3285 2081
Fragments arranged by size:
3285 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5413
StyI C'CwwG_G
Cuts at: 513 1065 2157 2168 2852 513
Size: 552 1092 11 684 3074
Fragments arranged by size:
3074 1092 684 552 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3440 3457 3610 3795 4240 325
Size: 1255 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 2113 3659
Size: 274 80 33 51 1443 72 64 1546
Cuts at: 3659 5371 96
Size: 1712 138
Fragments arranged by size:
1712 1546 1443 274 138 80 72 64
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5373 5405 5373
Size: 32 5381
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5413
XbaI T'CTAG_A
Cuts at: 2749 2749
Size: 5413
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5413
XmaI C'CCGG_G
Cuts at: 1870 2738 1870
Size: 868 4545
XmnI GAAnn'nnTTC
Cuts at: 2409 3542 2409
Size: 1133 4280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3221 276
Size: 53 83 186 936 1687 2468
Fragments arranged by size:
2468 1687 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI