Restriction map for
pFN21AE4025
(Circular) (Six-base) MAPSORT of: pFN21AE4025.seq Check: 1317 from: 1 to: 5401
pFN21AE4025 5401 bp
With 182 enzymes: SgfI *
March 28, 2012 13:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 2539 3464 4848 5116 1632
Size: 597 310 925 1384 268 1917
Fragments arranged by size:
1917 1384 925 597 310 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 2661 4232 2661
Size: 1571 3830
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5401
AlwNI CAG_nnn'CTG
Cuts at: 2264 3144 4716 2264
Size: 880 1572 2949
Fragments arranged by size:
2949 1572 880
ApaI G_GGCC'C
Cuts at: 2068 2068
Size: 5401
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2710 2936 3088 5197 1493
Size: 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 1870
Size: 73 783 4545
Fragments arranged by size:
4545 783 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2722 4177 618
Size: 325 217 627 254 681 1455 1842
Fragments arranged by size:
1842 1455 681 627 325 254 217
BanII G_rGCy'C
Cuts at: 1391 2012 2068 2720 1391
Size: 621 56 652 4072
Fragments arranged by size:
4072 652 621 56
BbeI G_GCGC'C
Cuts at: 2045 2045
Size: 5401
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2460 928
Size: 971 561 3869
Fragments arranged by size:
3869 971 561
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3592 3626 1903
Size: 34 1655 34 3678
Fragments arranged by size:
3678 1655 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2018 2818 2018
Size: 800 4601
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2347 3462 4618 1790
Size: 278 279 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 279 278
BmgBI CAC'GTC
Cuts at: 1318 2103 1318
Size: 785 4616
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 4090 442
Size: 416 53 83 399 24 1715 958 1753
Fragments arranged by size:
1753 1715 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 4061 1239
Size: 851 1971 2579
Fragments arranged by size:
2579 1971 851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2420 3526 4391 4689 2147
Size: 273 1106 865 298 2859
Fragments arranged by size:
2859 1106 865 298 273
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4064 882
Size: 617 2565 2219
Fragments arranged by size:
2565 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2167 3192 493
Size: 711 963 1025 2702
Fragments arranged by size:
2702 1025 963 711
BsaBI GATnn'nnATC
Cuts at: 2541 5395 2541
Size: 2854 2547
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 3208 3590 4225
Size: 53 83 186 936 509 1166 382 635
Cuts at: 4225 275
Size: 1451
Fragments arranged by size:
1451 1166 936 635 509 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2148 2661 3834 4232 4506 4653 5064 2148
Size: 513 1173 398 274 147 411 2485
Fragments arranged by size:
2485 1173 513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5176
Fragments arranged by size:
5176 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2417 1186
Size: 1231 4170
BseYI C'CCAG_C
Cuts at: 4604 5100 4604
Size: 496 4905
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4828
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3612 4640 1540
Size: 275 186 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 3462 3547 4618 2024
Size: 1438 85 1071 2807
Fragments arranged by size:
2807 1438 1071 85
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2024 2068 2347 2720 3462 3547
Size: 399 222 12 44 279 373 742 85
Cuts at: 3547 4618 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 279 222 85
44 12
BspEI T'CCGG_A
Cuts at: 2148 5064 2148
Size: 2916 2485
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 844
Size: 1912 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2661 4045 4232 1700
Size: 110 851 1384 187 2869
Fragments arranged by size:
2869 1384 851 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5401
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2731 3098 3483 3500 4952 5038 5050 5396
Size: 759 367 385 17 1452 86 12 346
Cuts at: 5396 1972
Size: 1977
Fragments arranged by size:
1977 1452 759 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3910
Fragments arranged by size:
3910 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2848 3737
Size: 54 1425 50 275 18 331 687 889
Cuts at: 3737 5366 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 687 331 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2644 3331 1353
Size: 1291 687 3423
Fragments arranged by size:
3423 1291 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2229 2539 3464 4061 4848 5116
Size: 393 458 139 310 925 597 787 268
Cuts at: 5116 1239
Size: 1524
Fragments arranged by size:
1524 925 787 597 458 393 310 268
139
EcoNI CCTnn'n_nnAGG
Cuts at: 2247 2247
Size: 5401
EcoO109I rG'GnC_Cy
Cuts at: 2065 2083 2065
Size: 18 5383
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 2045 4548 1678
Size: 367 2503 2531
Fragments arranged by size:
2531 2503 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373
Size: 32 135 32 2470 32 42 32 2626
Fragments arranged by size:
2626 2470 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KasI G'GCGC_C
Cuts at: 2041 2041
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4515 4699 1305
Size: 3210 184 2007
Fragments arranged by size:
3210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 10
Size: 54 1425 343 331 3248
Fragments arranged by size:
3248 1425 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 3718 3877 518
Size: 648 2193 359 159 2042
Fragments arranged by size:
2193 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2157 2817 3494 4642 4887 1606
Size: 425 126 660 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 425 245 126
NarI GG'CG_CC
Cuts at: 2042 2042
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3910
Fragments arranged by size:
3910 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5401
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 5401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PspOMI G'GGCC_C
Cuts at: 2064 2064
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2753 830
Size: 1923 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SanDI GG'GwC_CC
Cuts at: 2083 2083
Size: 5401
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SfcI C'TryA_G
Cuts at: 826 1045 2749 3884 4565 4756 826
Size: 219 1704 1135 681 191 1471
Fragments arranged by size:
1704 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2043 2043
Size: 5401
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2399 3505 4406 4668 820
Size: 197 926 183 273 1106 901 262 1553
Fragments arranged by size:
1553 1106 926 901 273 262 197 183
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 2503 1517
Size: 986 4415
StyI C'CwwG_G
Cuts at: 513 1065 2004 2840 513
Size: 552 939 836 3074
Fragments arranged by size:
3074 939 836 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3428 3445 3598 3783 4228 325
Size: 1255 1848 17 153 185 445 1498
Fragments arranged by size:
1848 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 3647 5359
Size: 274 80 33 51 1443 390 1280 1712
Cuts at: 5359 96
Size: 138
Fragments arranged by size:
1712 1443 1280 390 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 5361 5393 2143
Size: 32 3186 32 2151
Fragments arranged by size:
3186 2151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 2253 3530 2253
Size: 1277 4124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SrfI SwaI XcmI