Restriction map for
pFN21AE4017
(Circular) (Six-base) MAPSORT of: pFN21AE4017.seq Check: 7121 from: 1 to: 5296
pFN21AE4017 5296 bp
With 182 enzymes: SgfI *
March 28, 2012 13:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3106 278
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2639 1937
Size: 702 4594
Acc65I G'GTAC_C
Cuts at: 2617 2617
Size: 5296
AclI AA'CG_TT
Cuts at: 1563 3423 3796 1563
Size: 1860 373 3063
Fragments arranged by size:
3063 1860 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3359 4743 5011 1632
Size: 1727 1384 268 1917
Fragments arranged by size:
1917 1727 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5099
AflIII A'CryG_T
Cuts at: 1203 2442 4195 5172 1203
Size: 1239 1753 977 1327
Fragments arranged by size:
1753 1327 1239 977
AgeI A'CCGG_T
Cuts at: 4127 4127
Size: 5296
AhdI GACnn_n'nnGTC
Cuts at: 4025 4025
Size: 5296
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5296
AlwNI CAG_nnn'CTG
Cuts at: 3039 4611 3039
Size: 1572 3724
ApaI G_GGCC'C
Cuts at: 2270 2270
Size: 5296
ApaLI G'TGCA_C
Cuts at: 3353 4509 3353
Size: 1156 4140
ApoI r'AATT_y
Cuts at: 1493 2605 2831 2983 5092 1493
Size: 1112 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2468 3850 160
Size: 872 1436 1382 1606
Fragments arranged by size:
1606 1436 1382 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 2621 1870
Size: 73 205 473 4545
Fragments arranged by size:
4545 473 205 73
BamHI G'GATC_C
Cuts at: 2626 2993 2626
Size: 367 4929
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2053 2201 2617 4072
Size: 325 217 627 252 14 148 416 1455
Cuts at: 4072 618
Size: 1842
Fragments arranged by size:
1842 1455 627 416 325 252 217 148
14
BanII G_rGCy'C
Cuts at: 1391 2147 2270 2615 1391
Size: 756 123 345 4072
Fragments arranged by size:
4072 756 345 123
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 5296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 928
Size: 971 218 4107
Fragments arranged by size:
4107 971 218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2382 2416 3487 3521 1903
Size: 34 445 34 1071 34 3678
Fragments arranged by size:
3678 1071 445 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4398 3188
Size: 1210 4086
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3907 136
Size: 107 122 71 631 605 2235 1525
Fragments arranged by size:
2235 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5291 5291
Size: 5296
BlpI GC'TnA_GC
Cuts at: 2548 2713 2548
Size: 165 5131
Bme1580I G_kGCm'C
Cuts at: 1790 2204 2270 3357 4513 1790
Size: 414 66 1087 1156 2573
Fragments arranged by size:
2573 1156 1087 414 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5296
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3027 3985 442
Size: 416 53 83 399 24 1610 958 1753
Fragments arranged by size:
1753 1610 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 5296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2555 3956 1239
Size: 1316 1401 2579
Fragments arranged by size:
2579 1401 1316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2050 3421 4286 4584 2050
Size: 1371 865 298 2762
Fragments arranged by size:
2762 1371 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 3959 882
Size: 617 876 1584 2219
Fragments arranged by size:
2219 1584 876 617
BsaAI yAC'GTr
Cuts at: 493 1204 3087 493
Size: 711 1883 2702
Fragments arranged by size:
2702 1883 711
BsaBI GATnn'nnATC
Cuts at: 2240 5290 2240
Size: 3050 2246
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2103 2322 3103
Size: 53 83 186 936 507 63 219 781
Cuts at: 3103 3485 4120 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 781 635 507 382 219 186
83 63 53
BsaWI w'CCGG_w
Cuts at: 3729 4127 4401 4548 4959 3729
Size: 398 274 147 411 4066
Fragments arranged by size:
4066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2447 2477 1178
Size: 30 165 30 1044 30 3997
Fragments arranged by size:
3997 1044 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2468 1186
Size: 1282 4014
BseYI C'CCAG_C
Cuts at: 2089 2337 4499 4995 2089
Size: 248 2162 496 2390
Fragments arranged by size:
2390 2162 496 248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 1163
Size: 573 642 4081
Fragments arranged by size:
4081 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2746 3507 4535 1540
Size: 275 186 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 275 186
BsiHKAI G_wGCw'C
Cuts at: 2147 3357 3442 4513 2147
Size: 1210 85 1071 2930
Fragments arranged by size:
2930 1210 1071 85
BsmI GAATG_Cn'
Cuts at: 2807 2900 2807
Size: 93 5203
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2204 2270 2615 3357 3442 4513
Size: 399 357 57 66 345 742 85 1071
Cuts at: 4513 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 357 345 85 66
57
BspEI T'CCGG_A
Cuts at: 4959 4959
Size: 5296
BspHI T'CATG_A
Cuts at: 3185 4915 3185
Size: 1730 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2651 844
Size: 1807 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3183 1394
Size: 429 1360 3507
Fragments arranged by size:
3507 1360 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2487 3791 3965 65
Size: 1183 1239 1304 174 1396
Fragments arranged by size:
1396 1304 1239 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 3940 4127 1700
Size: 110 205 1925 187 2869
Fragments arranged by size:
2869 1925 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5296
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5296
BssSI C'ACGA_G
Cuts at: 3356 4368 3356
Size: 1012 4284
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5296
BstBI TT'CG_AA
Cuts at: 4100 4100
Size: 5296
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5296
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5296
BstYI r'GATC_y
Cuts at: 1972 2626 2993 3378 3395 4847 4933 4945 5291
Size: 654 367 385 17 1452 86 12 346
Cuts at: 5291 1972
Size: 1977
Fragments arranged by size:
1977 1452 654 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2035 2097 2193 2448 2514 513
Size: 552 970 62 96 255 66 3295
Fragments arranged by size:
3295 970 552 255 96 66 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4557
Fragments arranged by size:
4557 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3606 3626 2812
Size: 794 20 4482
Fragments arranged by size:
4482 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5261
DraI TTT'AAA
Cuts at: 2600 2600
Size: 5296
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4303 809
Size: 507 2987 1802
Fragments arranged by size:
2987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2743 3632
Size: 54 1425 50 275 18 183 730 889
Cuts at: 3632 5261 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 730 275 183 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2743 1537
Size: 275 931 4090
Fragments arranged by size:
4090 931 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2420 3226 1353
Size: 1067 806 3423
Fragments arranged by size:
3423 1067 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3883 4257 4403 387
Size: 1064 138 2294 374 146 1280
Fragments arranged by size:
2294 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2145 2145
Size: 5296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2555 3359 3956 4743 5011 1239
Size: 393 923 804 597 787 268 1524
Fragments arranged by size:
1524 923 804 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2267 2267
Size: 5296
EcoRI G'AATT_C
Cuts at: 2605 2983 2605
Size: 378 4918
FspI TGC'GCA
Cuts at: 3802 3802
Size: 5296
HaeII r_GCGC'y
Cuts at: 1678 2043 2532 4443 1678
Size: 365 489 1911 2531
Fragments arranged by size:
2531 1911 489 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2479 3937 3969 4011
Size: 32 135 32 875 32 1458 32 42
Cuts at: 4011 4043 1373
Size: 32 2626
Fragments arranged by size:
2626 1458 875 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2640 2886 669
Size: 988 281 702 246 3079
Fragments arranged by size:
3079 988 702 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5296
HpaI GTT'AAC
Cuts at: 2886 2886
Size: 5296
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 5296
KpnI G_GTAC'C
Cuts at: 2621 2621
Size: 5296
MfeI C'AATT_G
Cuts at: 2895 2895
Size: 5296
MluI A'CGCG_T
Cuts at: 5172 5172
Size: 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4410 4594 1305
Size: 3105 184 2007
Fragments arranged by size:
3105 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3474
Fragments arranged by size:
3474 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3254 3613 3772 518
Size: 648 843 1245 359 159 2042
Fragments arranged by size:
2042 1245 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2712 3389 4537 4782 1606
Size: 431 675 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 431 245
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 5296
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4744
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5296
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5296
NotI GC'GGCC_GC
Cuts at: 2743 2743
Size: 5296
NspI r_CATG'y
Cuts at: 1132 1229 2446 2654 4199 1132
Size: 97 1217 208 1545 2229
Fragments arranged by size:
2229 1545 1217 208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5296
PciI A'CATG_T
Cuts at: 2442 4195 2442
Size: 1753 3543
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5296
PfoI T'CCnGG_A
Cuts at: 2244 2244
Size: 5296
PmeI GTTT'AAAC
Cuts at: 2600 2600
Size: 5296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 4878 4910 3365
Size: 32 1481 32 3751
Fragments arranged by size:
3751 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5296
PsiI TTA'TAA
Cuts at: 2866 2866
Size: 5296
PspOMI G'GGCC_C
Cuts at: 2266 2266
Size: 5296
PstI C_TGCA'G
Cuts at: 830 2136 2383 2648 830
Size: 1306 247 265 3478
Fragments arranged by size:
3478 1306 265 247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5296
SacI G_AGCT'C
Cuts at: 2147 2147
Size: 5296
SacII CC_GC'GG
Cuts at: 2038 2038
Size: 5296
SalI G'TCGA_C
Cuts at: 1936 2638 1936
Size: 702 4594
SbfI CC_TGCA'GG
Cuts at: 2648 2648
Size: 5296
ScaI AGT'ACT
Cuts at: 3544 5256 3544
Size: 1712 3584
SfcI C'TryA_G
Cuts at: 826 1045 2132 2379 2644 3779 4460 4651 826
Size: 219 1087 247 265 1135 681 191 1471
Fragments arranged by size:
1471 1135 1087 681 265 247 219 191
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 5296
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5296
SmaI CCC'GGG
Cuts at: 1872 2150 2623 1872
Size: 278 473 4545
Fragments arranged by size:
4545 473 278
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2029 3400 4301 4563 820
Size: 197 926 86 1371 901 262 1553
Fragments arranged by size:
1553 1371 926 901 262 197 86
SnaBI TAC'GTA
Cuts at: 493 3087 493
Size: 2594 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5296
SphI G_CATG'C
Cuts at: 1132 2654 1132
Size: 1522 3774
SspI AAT'ATT
Cuts at: 5 52 3220 5
Size: 47 3168 2081
Fragments arranged by size:
3168 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5296
StyI C'CwwG_G
Cuts at: 513 1065 2735 513
Size: 552 1670 3074
Fragments arranged by size:
3074 1670 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 2594 3323 3340 3493 3678 4123
Size: 1255 771 243 729 17 153 185 445
Cuts at: 4123 325
Size: 1498
Fragments arranged by size:
1498 1255 771 729 445 243 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3542 5254 96
Size: 274 80 33 51 1443 1565 1712 138
Fragments arranged by size:
1712 1565 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5256 5288 5256
Size: 32 5264
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5296
XbaI T'CTAG_A
Cuts at: 2632 2632
Size: 5296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5296
XmaI C'CCGG_G
Cuts at: 1870 2148 2621 1870
Size: 278 473 4545
Fragments arranged by size:
4545 473 278
XmnI GAAnn'nnTTC
Cuts at: 3425 3425
Size: 5296
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3104 276
Size: 53 83 186 936 1570 2468
Fragments arranged by size:
2468 1570 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI
SexAI SfiI SrfI SwaI XcmI