Restriction map for
pFN21AE4009
(Circular) (Six-base) MAPSORT of: pFN21AE4009.seq Check: 5966 from: 1 to: 7189
pFN21AE4009 7189 bp
With 182 enzymes: SgfI *
March 28, 2012 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2683 2922 3079 2683
Size: 239 157 6793
Fragments arranged by size:
6793 239 157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4999 278
Size: 53 83 186 936 3463 2468
Fragments arranged by size:
3463 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2943 4365 4532 1937
Size: 1006 1422 167 4594
Fragments arranged by size:
4594 1422 1006 167
Acc65I G'GTAC_C
Cuts at: 4510 4510
Size: 7189
AclI AA'CG_TT
Cuts at: 1563 5316 5689 1563
Size: 3753 373 3063
Fragments arranged by size:
3753 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2100 2824 2995 5252 6636 6904 1632
Size: 468 724 171 2257 1384 268 1917
Fragments arranged by size:
2257 1917 1384 724 468 268 171
AfeI AGC'GCT
Cuts at: 3929 3929
Size: 7189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6992
AflIII A'CryG_T
Cuts at: 1203 2457 2631 6088 7065 1203
Size: 1254 174 3457 977 1327
Fragments arranged by size:
3457 1327 1254 977 174
AgeI A'CCGG_T
Cuts at: 6020 6020
Size: 7189
AhdI GACnn_n'nnGTC
Cuts at: 5918 5918
Size: 7189
AleI CACnn'nnGTG
Cuts at: 1166 2969 1166
Size: 1803 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3435 1184
Size: 2251 4938
AlwNI CAG_nnn'CTG
Cuts at: 2078 2561 2785 2874 3331 3346 4932 6504 2078
Size: 483 224 89 457 15 1586 1572 2763
Fragments arranged by size:
2763 1586 1572 483 457 224 89 15
ApaI G_GGCC'C
Cuts at: 3028 3998 4065 3028
Size: 970 67 6152
Fragments arranged by size:
6152 970 67
ApaLI G'TGCA_C
Cuts at: 3740 5246 6402 3740
Size: 1506 1156 4527
Fragments arranged by size:
4527 1506 1156
ApoI r'AATT_y
Cuts at: 1493 2018 2107 2720 4498 4724 4876 6985 1493
Size: 525 89 613 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 613 525 226 152 89
AseI AT'TA_AT
Cuts at: 160 1032 5743 160
Size: 872 4711 1606
Fragments arranged by size:
4711 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 2440 2831 2859 3263 3443 3752
Size: 73 297 200 391 28 404 180 309
Cuts at: 3752 4034 4076 4514 1870
Size: 282 42 438 4545
Fragments arranged by size:
4545 438 404 391 309 297 282 200
180 73 42 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4390 4423 4390
Size: 33 7156
BamHI G'GATC_C
Cuts at: 4519 4886 4519
Size: 367 6822
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 3646 3653 3936 4147
Size: 325 217 627 1811 48 7 283 211
Cuts at: 4147 4428 4510 5965 618
Size: 281 82 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 283 281 217
211 82 48 7
BanII G_rGCy'C
Cuts at: 1391 2358 2469 2702 2763 2838 3028 3270 3444
Size: 967 111 233 61 75 190 242 174
Cuts at: 3444 3759 3998 4065 4508 1391
Size: 315 239 67 443 4072
Fragments arranged by size:
4072 967 443 315 242 239 233 190
174 111 75 67 61
BbeI G_GCGC'C
Cuts at: 3940 3940
Size: 7189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3232 928
Size: 971 1333 4885
Fragments arranged by size:
4885 1333 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3142 3176 5380 5414 1903
Size: 34 1205 34 2204 34 3678
Fragments arranged by size:
3678 2204 1205 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5081 6291 4418
Size: 663 1210 5316
Fragments arranged by size:
5316 1210 663
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7189
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5800 136
Size: 107 122 71 631 605 4128 1525
Fragments arranged by size:
4128 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3372 3588 7184 3372
Size: 216 3596 3377
Fragments arranged by size:
3596 3377 216
BlpI GC'TnA_GC
Cuts at: 3668 4606 3668
Size: 938 6251
Bme1580I G_kGCm'C
Cuts at: 1790 3028 3424 3603 3651 3744 3998 4065 5250
Size: 1238 396 179 48 93 254 67 1185
Cuts at: 5250 6406 1790
Size: 1156 2573
Fragments arranged by size:
2573 1238 1185 1156 396 254 179 93
67 48
BmgBI CAC'GTC
Cuts at: 1318 2625 2848 1318
Size: 1307 223 5659
Fragments arranged by size:
5659 1307 223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4920 5878 442
Size: 416 53 83 399 24 3503 958 1753
Fragments arranged by size:
3503 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2296 2146
Size: 150 7039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2364 2921 3493 3923 5849 1239
Size: 910 215 557 572 430 1926 2579
Fragments arranged by size:
2579 1926 910 572 557 430 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 2747 4448 5314 6179 6477 2690
Size: 57 1701 866 865 298 3402
Fragments arranged by size:
3402 1701 866 865 298 57
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5852 882
Size: 617 4353 2219
Fragments arranged by size:
4353 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3175 3856 3988 4354 4980 493
Size: 711 1971 681 132 366 626 2702
Fragments arranged by size:
2702 1971 711 681 626 366 132
BsaBI GATnn'nnATC
Cuts at: 7183 7183
Size: 7189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4996 5378 6013
Size: 53 83 186 936 2404 1059 382 635
Cuts at: 6013 275
Size: 1451
Fragments arranged by size:
2404 1451 1059 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3147 5622 6020 6294 6441 6852 3147
Size: 2475 398 274 147 411 3484
Fragments arranged by size:
3484 2475 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2496 2526 2648 2678 3122
Size: 30 165 30 1093 30 122 30 444
Cuts at: 3122 3152 3200 3230 3491 3521 4376 4406 1178
Size: 30 48 30 261 30 855 30 3961
Fragments arranged by size:
3961 1093 855 444 261 165 122 48
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2239 2363 2366 3143 3208 3236 3410 3413
Size: 1053 124 3 777 65 28 174 3
Cuts at: 3413 3496 4064 4096 4223 4400 1186
Size: 83 568 32 127 177 3975
Fragments arranged by size:
3975 1053 777 568 177 174 127 124
83 65 32 28 3 3
BseYI C'CCAG_C
Cuts at: 2637 2994 3027 3053 3286 3423 3630 4412 6392
Size: 357 33 26 233 137 207 782 1980
Cuts at: 6392 6888 2637
Size: 496 2938
Fragments arranged by size:
2938 1980 782 496 357 233 207 137
33 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 2840 2894 3878 4024 4214 1163
Size: 573 891 213 54 984 146 190 4138
Fragments arranged by size:
4138 984 891 573 213 190 146 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3569 3889 4639 5400 6428 1540
Size: 275 186 1568 320 750 761 1028 2301
Fragments arranged by size:
2301 1568 1028 761 750 320 275 186
BsiHKAI G_wGCw'C
Cuts at: 2469 2763 2838 3270 3444 3744 3759 5250 5335
Size: 294 75 432 174 300 15 1491 85
Cuts at: 5335 6406 2469
Size: 1071 3252
Fragments arranged by size:
3252 1491 1071 432 300 294 174 85
75 15
BsmI GAATG_Cn'
Cuts at: 4700 4793 4700
Size: 93 7096
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2358 2469 2702 2763 2838 3028 3270
Size: 399 568 111 233 61 75 190 242
Cuts at: 3270 3424 3444 3603 3651 3744 3759 3998 4065
Size: 154 20 159 48 93 15 239 67
Cuts at: 4065 4508 5250 5335 6406 1391
Size: 443 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 568 443 399 242 239
233 190 159 154 111 93 85 75
67 61 48 20 15
BspEI T'CCGG_A
Cuts at: 6852 6852
Size: 7189
BspHI T'CATG_A
Cuts at: 5078 6808 5078
Size: 1730 5459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2457 2667 2683 2922 3079 3783 4170 4544
Size: 1613 210 16 239 157 704 387 374
Cuts at: 4544 844
Size: 3489
Fragments arranged by size:
3489 1613 704 387 374 239 210 157
16
BsrBI CCG'CTC
Cuts at: 1394 1823 3565 3579 5076 1394
Size: 429 1742 14 1497 3507
Fragments arranged by size:
3507 1742 1497 429 14
BsrDI GCAATG_nn'
Cuts at: 65 1248 2302 2338 5684 5858 65
Size: 1183 1054 36 3346 174 1396
Fragments arranged by size:
3346 1396 1183 1054 174 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 2309 3252 3933 5833 6020 1700
Size: 110 499 943 681 1900 187 2869
Fragments arranged by size:
2869 1900 943 681 499 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7189
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7189
BssSI C'ACGA_G
Cuts at: 3767 5249 6261 3767
Size: 1482 1012 4695
Fragments arranged by size:
4695 1482 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3346 794
Size: 2552 4637
BstBI TT'CG_AA
Cuts at: 5993 5993
Size: 7189
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7189
BstXI CCAn_nnnn'nTGG
Cuts at: 2297 2710 2297
Size: 413 6776
BstYI r'GATC_y
Cuts at: 1972 3372 3588 3624 4104 4519 4886 5271 5288
Size: 1400 216 36 480 415 367 385 17
Cuts at: 5288 6740 6826 6838 7184 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1400 480 415 385 367 346
216 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4209 513
Size: 552 3144 3493
Fragments arranged by size:
3493 3144 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2205 3145 487
Size: 683 56 979 940 4531
Fragments arranged by size:
4531 979 940 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2504 2875 3422 3475 4067 4705 5499 5519
Size: 356 371 547 53 592 638 794 20
Cuts at: 5519 2148
Size: 3818
Fragments arranged by size:
3818 794 638 592 547 371 356 53
20
ClaI AT'CG_AT
Cuts at: 1554 4419 1554
Size: 2865 4324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4461 4496 529
Size: 35 3897 35 3222
Fragments arranged by size:
3897 3222 35 35
DraI TTT'AAA
Cuts at: 4493 4493
Size: 7189
DraIII CAC_nnn'GTG
Cuts at: 2134 3178 3856 4354 2134
Size: 1044 678 498 4969
Fragments arranged by size:
4969 1044 678 498
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6196 809
Size: 507 4880 1802
Fragments arranged by size:
4880 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2715 2886 3058
Size: 54 1425 50 275 18 885 171 172
Cuts at: 3058 3100 3566 4636 5525 7154 8
Size: 42 466 1070 889 1629 43
Fragments arranged by size:
1629 1425 1070 889 885 466 275 172
171 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 3566 4636 1537
Size: 275 1754 1070 4090
Fragments arranged by size:
4090 1754 1070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 2831 2843 3263 3275 3764 4262 5119
Size: 863 615 12 420 12 489 498 857
Cuts at: 5119 1353
Size: 3423
Fragments arranged by size:
3423 863 857 615 498 489 420 12
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3836 4229 4334 5776 6150 6296
Size: 1064 138 2247 393 105 1442 374 146
Cuts at: 6296 387
Size: 1280
Fragments arranged by size:
2247 1442 1280 1064 393 374 146 138
105
EcoICRI GAG'CTC
Cuts at: 2467 2761 2836 3268 3442 3757 2467
Size: 294 75 432 174 315 5899
Fragments arranged by size:
5899 432 315 294 174 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2149 2364 2824 2921 2995 3493
Size: 393 468 49 215 460 97 74 498
Cuts at: 3493 3923 5252 5849 6636 6904 1239
Size: 430 1329 597 787 268 1524
Fragments arranged by size:
1524 1329 787 597 498 468 460 430
393 268 215 97 74 49
EcoO109I rG'GnC_Cy
Cuts at: 2446 2797 3133 3190 3448 3995 4061 4062 2446
Size: 351 336 57 258 547 66 1 5573
Fragments arranged by size:
5573 547 351 336 258 66 57 1
EcoRI G'AATT_C
Cuts at: 2018 2107 4498 4876 2018
Size: 89 2391 378 4331
Fragments arranged by size:
4331 2391 378 89
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4126 4126
Size: 7189
FspI TGC'GCA
Cuts at: 3779 5695 3779
Size: 1916 5273
HaeII r_GCGC'y
Cuts at: 1678 3931 3940 3961 6336 1678
Size: 2253 9 21 2375 2531
Fragments arranged by size:
2531 2375 2253 21 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 3122 3154 3200
Size: 32 135 32 1076 32 442 32 46
Cuts at: 3200 3232 3512 3544 5830 5862 5904 5936 1373
Size: 32 280 32 2286 32 42 32 2626
Fragments arranged by size:
2626 2286 1076 442 280 135 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4533 4779 669
Size: 988 281 2595 246 3079
Fragments arranged by size:
3079 2595 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7189
HpaI GTT'AAC
Cuts at: 4779 4779
Size: 7189
KasI G'GCGC_C
Cuts at: 3936 3936
Size: 7189
KpnI G_GTAC'C
Cuts at: 4514 4514
Size: 7189
MfeI C'AATT_G
Cuts at: 4788 4788
Size: 7189
MluI A'CGCG_T
Cuts at: 7065 7065
Size: 7189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2652 6303 6487 1305
Size: 1347 3651 184 2007
Fragments arranged by size:
3651 2007 1347 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2717 10
Size: 54 1425 343 885 4482
Fragments arranged by size:
4482 1425 885 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 2969 3530 5147 5506 5665 518
Size: 648 1542 261 561 1617 359 159 2042
Fragments arranged by size:
2042 1617 1542 648 561 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2641 2998 3031 3057 3303 3343 3798
Size: 576 459 357 33 26 246 40 455
Cuts at: 3798 3820 3862 4605 5282 6430 6675 1606
Size: 22 42 743 677 1148 245 2120
Fragments arranged by size:
2120 1148 743 677 576 459 455 357
246 245 42 40 33 26 22
NaeI GCC'GGC
Cuts at: 2311 3935 2311
Size: 1624 5565
NarI GG'CG_CC
Cuts at: 3937 3937
Size: 7189
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6637
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7189
NgoMIV G'CCGG_C
Cuts at: 2309 3933 2309
Size: 1624 5565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7189
NotI GC'GGCC_GC
Cuts at: 4636 4636
Size: 7189
NspI r_CATG'y
Cuts at: 1132 1229 2263 2611 2635 3391 4547 6092 1132
Size: 97 1034 348 24 756 1156 1545 2229
Fragments arranged by size:
2229 1545 1156 1034 756 348 97 24
PasI CC'CwG_GG
Cuts at: 1348 3805 1348
Size: 2457 4732
PciI A'CATG_T
Cuts at: 2631 6088 2631
Size: 3457 3732
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3109 3178 4354 1108
Size: 2001 69 1176 3943
Fragments arranged by size:
3943 2001 1176 69
PfoI T'CCnGG_A
Cuts at: 2111 3108 3204 4044 2111
Size: 997 96 840 5256
Fragments arranged by size:
5256 997 840 96
PmeI GTTT'AAAC
Cuts at: 4493 4493
Size: 7189
PmlI CAC'GTG
Cuts at: 3175 3988 3175
Size: 813 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5258 5290 6771 6803 5258
Size: 32 1481 32 5644
Fragments arranged by size:
5644 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2446 2797 3190 2446
Size: 351 393 6445
Fragments arranged by size:
6445 393 351
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7189
PsiI TTA'TAA
Cuts at: 4759 4759
Size: 7189
PspOMI G'GGCC_C
Cuts at: 3024 3994 4061 3024
Size: 970 67 6152
Fragments arranged by size:
6152 970 67
PspXI vC'TCGA_Gb
Cuts at: 3443 3443
Size: 7189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2025 2057 2025
Size: 32 7157
PstI C_TGCA'G
Cuts at: 830 2160 2870 2919 3351 3414 3797 4284 4541
Size: 1330 710 49 432 63 383 487 257
Cuts at: 4541 830
Size: 3478
Fragments arranged by size:
3478 1330 710 487 432 383 257 63
49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7189
PvuII CAG'CTG
Cuts at: 2182 2641 2998 3303 3820 3862 2182
Size: 459 357 305 517 42 5509
Fragments arranged by size:
5509 517 459 357 305 42
SacI G_AGCT'C
Cuts at: 2469 2763 2838 3270 3444 3759 2469
Size: 294 75 432 174 315 5899
Fragments arranged by size:
5899 432 315 294 174 75
SalI G'TCGA_C
Cuts at: 1936 4531 1936
Size: 2595 4594
SapI GCTCTTCn'nnn_
Cuts at: 2831 2843 3263 3275 3764 2831
Size: 12 420 12 489 6256
Fragments arranged by size:
6256 489 420 12 12
SbfI CC_TGCA'GG
Cuts at: 2160 4541 2160
Size: 2381 4808
ScaI AGT'ACT
Cuts at: 5437 7149 5437
Size: 1712 5477
SfcI C'TryA_G
Cuts at: 826 1045 2156 2866 2915 3347 3410 3793 4280
Size: 219 1111 710 49 432 63 383 487
Cuts at: 4280 4537 5672 6353 6544 826
Size: 257 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 710 681 487 432 383
257 219 191 63 49
SfoI GGC'GCC
Cuts at: 3938 3938
Size: 7189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7189
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7189
SmaI CCC'GGG
Cuts at: 1872 2861 4516 1872
Size: 989 1655 4545
Fragments arranged by size:
4545 1655 989
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2669 2762 3443 4463 5293 6194
Size: 197 926 726 93 681 1020 830 901
Cuts at: 6194 6456 820
Size: 262 1553
Fragments arranged by size:
1553 1020 926 901 830 726 681 262
197 93
SnaBI TAC'GTA
Cuts at: 493 4980 493
Size: 4487 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7189
SphI G_CATG'C
Cuts at: 1132 2263 4547 1132
Size: 1131 2284 3774
Fragments arranged by size:
3774 2284 1131
SspI AAT'ATT
Cuts at: 5 52 5113 5
Size: 47 5061 2081
Fragments arranged by size:
5061 2081 47
StuI AGG'CCT
Cuts at: 1517 3404 1517
Size: 1887 5302
StyI C'CwwG_G
Cuts at: 513 1065 2290 4110 4628 513
Size: 552 1225 1820 518 3074
Fragments arranged by size:
3074 1820 1225 552 518
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3278 5216 5233 5386 5571 6016 325
Size: 1255 1698 1938 17 153 185 445 1498
Fragments arranged by size:
1938 1698 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3297 3786 4284
Size: 274 80 33 51 1443 1320 489 498
Cuts at: 4284 5435 7147 96
Size: 1151 1712 138
Fragments arranged by size:
1712 1443 1320 1151 498 489 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2496 2528 4376 4408 7149 7181 2496
Size: 32 1848 32 2741 32 2504
Fragments arranged by size:
2741 2504 1848 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2141 2207 3890 1316
Size: 825 66 1683 4615
Fragments arranged by size:
4615 1683 825 66
XbaI T'CTAG_A
Cuts at: 4525 4525
Size: 7189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2710 2896 2710
Size: 186 7003
XhoI C'TCGA_G
Cuts at: 1943 3443 1943
Size: 1500 5689
XmaI C'CCGG_G
Cuts at: 1870 2859 4514 1870
Size: 989 1655 4545
Fragments arranged by size:
4545 1655 989
XmnI GAAnn'nnTTC
Cuts at: 2040 2600 3005 5318 2040
Size: 560 405 2313 3911
Fragments arranged by size:
3911 2313 560 405
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4997 276
Size: 53 83 186 936 3463 2468
Fragments arranged by size:
3463 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI
BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI
PacI RsrII SacII SanDI SexAI SfiI SrfI SwaI