Restriction map for
pFN21AE3993
(Circular) (Six-base) MAPSORT of: pFN21AE3993.seq Check: 5240 from: 1 to: 5527
pFN21AE3993 5527 bp
With 182 enzymes: SgfI *
March 28, 2012 11:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2788 2870 1937
Size: 851 82 4594
Fragments arranged by size:
4594 851 82
Acc65I G'GTAC_C
Cuts at: 2453 2848 2453
Size: 395 5132
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 2588 3590 4974 5242 1632
Size: 818 138 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1002 818 268 138
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 3270 4842 3270
Size: 1572 3955
ApaI G_GGCC'C
Cuts at: 2287 2287
Size: 5527
ApaLI G'TGCA_C
Cuts at: 2691 3584 4740 2691
Size: 893 1156 3478
Fragments arranged by size:
3478 1156 893
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2334 4081 160
Size: 872 1302 1747 1606
Fragments arranged by size:
1747 1606 1302 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2852 1870
Size: 73 909 4545
Fragments arranged by size:
4545 909 73
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2453 2848 4303 618
Size: 325 217 627 666 395 1455 1842
Fragments arranged by size:
1842 1455 666 627 395 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2287 2846 1391
Size: 649 247 559 4072
Fragments arranged by size:
4072 649 559 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2646 928
Size: 971 747 3809
Fragments arranged by size:
3809 971 747
BbvCI CC'TCA_GC
Cuts at: 2427 2427
Size: 5527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3718 3752 1903
Size: 34 1781 34 3678
Fragments arranged by size:
3678 1781 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3419 4629 3419
Size: 1210 4317
BclI T'GATC_A
Cuts at: 1548 2568 1548
Size: 1020 4507
BfrBI ATG'CAT
Cuts at: 2275 2275
Size: 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 4138 136
Size: 107 122 71 631 605 397 2069 1525
Fragments arranged by size:
2069 1525 631 605 397 122 107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2944 2944
Size: 5527
Bme1580I G_kGCm'C
Cuts at: 1790 2287 2695 3588 4744 1790
Size: 497 408 893 1156 2573
Fragments arranged by size:
2573 1156 893 497 408
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3258 4216 442
Size: 416 53 83 399 24 1841 958 1753
Fragments arranged by size:
1841 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2435 4187 1239
Size: 785 411 1752 2579
Fragments arranged by size:
2579 1752 785 411
Bpu10I CC'TnA_GC
Cuts at: 2034 2427 2034
Size: 393 5134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3318 493
Size: 711 2114 2702
Fragments arranged by size:
2702 2114 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3334 3716 4351 275
Size: 53 83 186 936 1801 382 635 1451
Fragments arranged by size:
1801 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 1186
Size: 1213 4314
BseYI C'CCAG_C
Cuts at: 2075 4730 5226 2075
Size: 2655 496 2376
Fragments arranged by size:
2655 2376 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4954
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2695 3588 3673 4744 2040
Size: 655 893 85 1071 2823
Fragments arranged by size:
2823 1071 893 655 85
BsmI GAATG_Cn'
Cuts at: 3038 3131 3038
Size: 93 5434
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2287 2695 2846 3588 3673 4744
Size: 399 250 247 408 151 742 85 1071
Cuts at: 4744 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 408 399 250 247 151
85
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 3416 5146 3416
Size: 1730 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2882 844
Size: 2038 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 1394
Size: 429 1591 3507
Fragments arranged by size:
3507 1591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 2353 4022 4196 65
Size: 1183 1041 64 1669 174 1396
Fragments arranged by size:
1669 1396 1183 1041 174 64
BsrFI r'CCGG_y
Cuts at: 1700 1810 4171 4358 1700
Size: 110 2361 187 2869
Fragments arranged by size:
2869 2361 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 3587 4599 3587
Size: 1012 4515
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522
Size: 885 367 385 17 1452 86 12 346
Cuts at: 5522 1972
Size: 1977
Fragments arranged by size:
1977 1452 885 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2672 2672
Size: 5527
BtgI C'CryG_G
Cuts at: 513 1065 2064 513
Size: 552 999 3976
Fragments arranged by size:
3976 999 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3837 3857 3043
Size: 794 20 4713
Fragments arranged by size:
4713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5492
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DraIII CAC_nnn'GTG
Cuts at: 2092 2444 2092
Size: 352 5175
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2399 4534 809
Size: 507 1083 2135 1802
Fragments arranged by size:
2135 1802 1083 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 2278 2974
Size: 54 1425 50 275 18 298 150 696
Cuts at: 2974 3863 5492 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 696 298 275 150 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 2262 3457 1353
Size: 687 222 1195 3423
Fragments arranged by size:
3423 1195 687 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 2038
Size: 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2435 2450 2588 3590 4187 4974
Size: 393 392 411 15 138 1002 597 787
Cuts at: 4974 5242 1239
Size: 268 1524
Fragments arranged by size:
1524 1002 787 597 411 393 392 268
138 15
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 4674 1678
Size: 2996 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4168 4200 4242 4274 1373
Size: 32 135 32 2596 32 42 32 2626
Fragments arranged by size:
2626 2596 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 2789 2871 3117 669
Size: 988 281 496 355 82 246 3079
Fragments arranged by size:
3079 988 496 355 281 246 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2457 2852 2457
Size: 395 5132
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4641 4825 1305
Size: 3336 184 2007
Fragments arranged by size:
3336 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 3485 3844 4003 518
Size: 648 1656 663 359 159 2042
Fragments arranged by size:
2042 1656 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2943 3620 4768 5013 1606
Size: 460 877 677 1148 245 2120
Fragments arranged by size:
2120 1148 877 677 460 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4975
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NsiI A_TGCA'T
Cuts at: 2277 2277
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5527
PfoI T'CCnGG_A
Cuts at: 2040 2412 2511 2040
Size: 372 99 5056
Fragments arranged by size:
5056 372 99
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2283 2283
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2170 2879 830
Size: 1340 709 3478
Fragments arranged by size:
3478 1340 709
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
SacI G_AGCT'C
Cuts at: 2040 2040
Size: 5527
SacII CC_GC'GG
Cuts at: 2067 2067
Size: 5527
SalI G'TCGA_C
Cuts at: 1936 2787 2869 1936
Size: 851 82 4594
Fragments arranged by size:
4594 851 82
SapI GCTCTTCn'nnn_
Cuts at: 2262 2262
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2166 2223 2875 4010 4691 4882 826
Size: 219 1121 57 652 1135 681 191 1471
Fragments arranged by size:
1471 1135 1121 681 652 219 191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3631 4532 4794 820
Size: 197 926 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2966 513
Size: 552 1901 3074
Fragments arranged by size:
3074 1901 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3554 3571 3724 3909 4354 325
Size: 1255 1974 17 153 185 445 1498
Fragments arranged by size:
1974 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3773 5485 96
Size: 274 80 33 51 1443 1796 1712 138
Fragments arranged by size:
1796 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5487 5519 5487
Size: 32 5495
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2203 2863 2203
Size: 660 4867
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 2546 3656 2546
Size: 1110 4417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI