Restriction map for
pFN21AE3990
(Circular) (Six-base) MAPSORT of: pFN21AE3990.seq Check: 3235 from: 1 to: 5329
pFN21AE3990 5329 bp
With 182 enzymes: SgfI *
March 28, 2012 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2514 2514
Size: 5329
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3139 278
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 2672 1937
Size: 223 512 4594
Fragments arranged by size:
4594 512 223
Acc65I G'GTAC_C
Cuts at: 2650 2650
Size: 5329
AclI AA'CG_TT
Cuts at: 1563 3456 3829 1563
Size: 1893 373 3063
Fragments arranged by size:
3063 1893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3392 4776 5044 1632
Size: 1760 1384 268 1917
Fragments arranged by size:
1917 1760 1384 268
AfeI AGC'GCT
Cuts at: 2285 2285
Size: 5329
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5132
AflIII A'CryG_T
Cuts at: 1203 2623 4228 5205 1203
Size: 1420 1605 977 1327
Fragments arranged by size:
1605 1420 1327 977
AgeI A'CCGG_T
Cuts at: 4160 4160
Size: 5329
AhdI GACnn_n'nnGTC
Cuts at: 4058 4058
Size: 5329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2189 1184
Size: 1005 4324
AlwNI CAG_nnn'CTG
Cuts at: 2550 3072 4644 2550
Size: 522 1572 3235
Fragments arranged by size:
3235 1572 522
ApaI G_GGCC'C
Cuts at: 2248 2248
Size: 5329
ApaLI G'TGCA_C
Cuts at: 3386 4542 3386
Size: 1156 4173
ApoI r'AATT_y
Cuts at: 1493 2638 2864 3016 5125 1493
Size: 1145 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3883 160
Size: 872 2851 1606
Fragments arranged by size:
2851 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 1870
Size: 73 711 4545
Fragments arranged by size:
4545 711 73
BamHI G'GATC_C
Cuts at: 2659 3026 2659
Size: 367 4962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2650 4105 618
Size: 325 217 627 579 284 1455 1842
Fragments arranged by size:
1842 1455 627 579 325 284 217
BanII G_rGCy'C
Cuts at: 1391 2248 2648 1391
Size: 857 400 4072
Fragments arranged by size:
4072 857 400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2153 2456 928
Size: 971 254 303 3801
Fragments arranged by size:
3801 971 303 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2219 2253 3520 3554 1903
Size: 34 282 34 1267 34 3678
Fragments arranged by size:
3678 1267 282 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2172 3221 4431 2172
Size: 1049 1210 3070
Fragments arranged by size:
3070 1210 1049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3940 136
Size: 107 122 71 631 605 2268 1525
Fragments arranged by size:
2268 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2184 5324 2184
Size: 3140 2189
BlpI GC'TnA_GC
Cuts at: 2746 2746
Size: 5329
Bme1580I G_kGCm'C
Cuts at: 1790 2248 3390 4546 1790
Size: 458 1142 1156 2573
Fragments arranged by size:
2573 1156 1142 458
BmgBI CAC'GTC
Cuts at: 1318 2320 1318
Size: 1002 4327
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3060 4018 442
Size: 416 53 83 399 24 1643 958 1753
Fragments arranged by size:
1753 1643 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2386 2122
Size: 264 5065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3989 1239
Size: 2750 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3454 4319 4617 3454
Size: 865 298 4166
Fragments arranged by size:
4166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3992 882
Size: 617 2493 2219
Fragments arranged by size:
2493 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3120 493
Size: 711 1916 2702
Fragments arranged by size:
2702 1916 711
BsaBI GATnn'nnATC
Cuts at: 2183 5323 2183
Size: 3140 2189
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2474 3136 3518 4153
Size: 53 83 186 936 941 662 382 635
Cuts at: 4153 275
Size: 1451
Fragments arranged by size:
1451 941 936 662 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3762 4160 4434 4581 4992 3762
Size: 398 274 147 411 4099
Fragments arranged by size:
4099 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5104
Fragments arranged by size:
5104 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 2524 1186
Size: 912 426 3991
Fragments arranged by size:
3991 912 426
BseYI C'CCAG_C
Cuts at: 4532 5028 4532
Size: 496 4833
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2577 1163
Size: 573 841 3915
Fragments arranged by size:
3915 841 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2779 3540 4568 1540
Size: 275 186 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3390 3475 4546 3390
Size: 85 1071 4173
Fragments arranged by size:
4173 1071 85
BsmI GAATG_Cn'
Cuts at: 2840 2933 2840
Size: 93 5236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2648 3390 3475 4546 1391
Size: 399 458 400 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 400 399 85
BspEI T'CCGG_A
Cuts at: 4992 4992
Size: 5329
BspHI T'CATG_A
Cuts at: 2301 3218 4948 2301
Size: 917 1730 2682
Fragments arranged by size:
2682 1730 917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 2514 2684 844
Size: 1234 436 170 3489
Fragments arranged by size:
3489 1234 436 170
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 1394
Size: 429 1393 3507
Fragments arranged by size:
3507 1393 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3824 3998 65
Size: 1183 2576 174 1396
Fragments arranged by size:
2576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3973 4160 1700
Size: 110 2163 187 2869
Fragments arranged by size:
2869 2163 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5329
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5329
BssSI C'ACGA_G
Cuts at: 3389 4401 3389
Size: 1012 4317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2070 794
Size: 1276 4053
BstBI TT'CG_AA
Cuts at: 4133 4133
Size: 5329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5329
BstXI CCAn_nnnn'nTGG
Cuts at: 2442 2442
Size: 5329
BstYI r'GATC_y
Cuts at: 1972 2184 2483 2659 3026 3411 3428 4880 4966
Size: 212 299 176 367 385 17 1452 86
Cuts at: 4966 4978 5324 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 299 212 176
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3838
Fragments arranged by size:
3838 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4590
Fragments arranged by size:
4590 683 56
BtsI GCAGTG_nn'
Cuts at: 2193 2345 2845 3639 3659 2193
Size: 152 500 794 20 3863
Fragments arranged by size:
3863 794 500 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5294
DraI TTT'AAA
Cuts at: 2633 2633
Size: 5329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4336 809
Size: 507 3020 1802
Fragments arranged by size:
3020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2776 3665 5294
Size: 54 1425 50 275 18 946 889 1629
Cuts at: 5294 8
Size: 43
Fragments arranged by size:
1629 1425 946 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2776 1537
Size: 275 964 4090
Fragments arranged by size:
4090 964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2526 3259 1353
Size: 1173 733 3423
Fragments arranged by size:
3423 1173 733
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3916 4290 4436 387
Size: 1064 138 2327 374 146 1280
Fragments arranged by size:
2327 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3392 3989 4776 5044 1239
Size: 393 1760 597 787 268 1524
Fragments arranged by size:
1760 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2638 3016 2638
Size: 378 4951
FspI TGC'GCA
Cuts at: 3835 3835
Size: 5329
HaeII r_GCGC'y
Cuts at: 1678 2287 4476 1678
Size: 609 2189 2531
Fragments arranged by size:
2531 2189 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 3970 4002 4044
Size: 32 135 32 755 32 1611 32 42
Cuts at: 4044 4076 1373
Size: 32 2626
Fragments arranged by size:
2626 1611 755 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 2673 2919 669
Size: 988 281 196 539 246 3079
Fragments arranged by size:
3079 988 539 281 246 196
HindIII A'AGCT_T
Cuts at: 748 2423 748
Size: 1675 3654
HpaI GTT'AAC
Cuts at: 2919 2919
Size: 5329
KpnI G_GTAC'C
Cuts at: 2654 2654
Size: 5329
MfeI C'AATT_G
Cuts at: 2928 2928
Size: 5329
MluI A'CGCG_T
Cuts at: 2623 5205 2623
Size: 2582 2747
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4443 4627 1305
Size: 3138 184 2007
Fragments arranged by size:
3138 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3507
Fragments arranged by size:
3507 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 3287 3646 3805 518
Size: 648 1005 1116 359 159 2042
Fragments arranged by size:
2042 1116 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2550 2745 3422 4570 4815 1606
Size: 944 195 677 1148 245 2120
Fragments arranged by size:
2120 1148 944 677 245 195
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3838
Fragments arranged by size:
3838 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5329
NotI GC'GGCC_GC
Cuts at: 2776 2776
Size: 5329
NspI r_CATG'y
Cuts at: 1132 1229 2687 4232 1132
Size: 97 1458 1545 2229
Fragments arranged by size:
2229 1545 1458 97
PasI CC'CwG_GG
Cuts at: 1348 2248 1348
Size: 900 4429
PciI A'CATG_T
Cuts at: 4228 4228
Size: 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5329
PfoI T'CCnGG_A
Cuts at: 2417 2417
Size: 5329
PmeI GTTT'AAAC
Cuts at: 2633 2633
Size: 5329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 4911 4943 3398
Size: 32 1481 32 3784
Fragments arranged by size:
3784 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5329
PsiI TTA'TAA
Cuts at: 2899 2899
Size: 5329
PspOMI G'GGCC_C
Cuts at: 2244 2244
Size: 5329
PstI C_TGCA'G
Cuts at: 830 2200 2681 830
Size: 1370 481 3478
Fragments arranged by size:
3478 1370 481
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5329
PvuII CAG'CTG
Cuts at: 2550 2550
Size: 5329
SalI G'TCGA_C
Cuts at: 1936 2671 1936
Size: 735 4594
SbfI CC_TGCA'GG
Cuts at: 2681 2681
Size: 5329
ScaI AGT'ACT
Cuts at: 2234 3577 5289 2234
Size: 1343 1712 2274
Fragments arranged by size:
2274 1712 1343
SfcI C'TryA_G
Cuts at: 826 1045 2196 2677 3812 4493 4684 826
Size: 219 1151 481 1135 681 191 1471
Fragments arranged by size:
1471 1151 1135 681 481 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5329
SmaI CCC'GGG
Cuts at: 1872 2656 1872
Size: 784 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3433 4334 4596 820
Size: 197 926 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3120 493
Size: 2627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5329
SphI G_CATG'C
Cuts at: 1132 2687 1132
Size: 1555 3774
SspI AAT'ATT
Cuts at: 5 52 3253 5
Size: 47 3201 2081
Fragments arranged by size:
3201 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5329
StyI C'CwwG_G
Cuts at: 513 1065 2004 2768 513
Size: 552 939 764 3074
Fragments arranged by size:
3074 939 764 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3373 3526 3711 4156 325
Size: 1255 1776 17 153 185 445 1498
Fragments arranged by size:
1776 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2232 3575 5287
Size: 274 80 33 51 1443 255 1343 1712
Cuts at: 5287 96
Size: 138
Fragments arranged by size:
1712 1443 1343 274 255 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5289 5321 5289
Size: 32 5297
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5329
XbaI T'CTAG_A
Cuts at: 2665 2665
Size: 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5329
XmaI C'CCGG_G
Cuts at: 1870 2654 1870
Size: 784 4545
XmnI GAAnn'nnTTC
Cuts at: 3458 3458
Size: 5329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3137 276
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI