Restriction map for
pFN21AE3982
(Circular) (Six-base) MAPSORT of: pFN21AE3982.seq Check: 3198 from: 1 to: 5692
pFN21AE3982 5692 bp
With 182 enzymes: SgfI *
March 28, 2012 11:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2055 3035 1937
Size: 118 980 4594
Fragments arranged by size:
4594 980 118
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 2963 3819 4192 1563
Size: 1400 856 373 3063
Fragments arranged by size:
3063 1400 856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2990 3755 5139 5407 1632
Size: 1358 765 1384 268 1917
Fragments arranged by size:
1917 1384 1358 765 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 3435 5007 3435
Size: 1572 4120
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2174 2595 2765 2974 3001 3227 3379 5488
Size: 681 421 170 209 27 226 152 2109
Cuts at: 5488 1493
Size: 1697
Fragments arranged by size:
2109 1697 681 421 226 209 170 152
27
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2480 3013 4468 618
Size: 325 217 627 693 533 1455 1842
Fragments arranged by size:
1842 1455 693 627 533 325 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2498 928
Size: 971 599 4122
Fragments arranged by size:
4122 971 599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2698 2915 3584 4794 2698
Size: 217 669 1210 3596
Fragments arranged by size:
3596 1210 669 217
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2492 2516 2936 4352 1239
Size: 1253 24 420 1416 2579
Fragments arranged by size:
2579 1416 1253 420 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2350 3483 493
Size: 711 1146 1133 2702
Fragments arranged by size:
2702 1146 1133 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 2373 2403 2420
Size: 30 165 30 931 30 9 30 17
Cuts at: 2420 2450 1178
Size: 30 4420
Fragments arranged by size:
4420 931 165 30 30 30 30 30
17 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2604 1186
Size: 1418 4274
BseYI C'CCAG_C
Cuts at: 2490 4895 5391 2490
Size: 2405 496 2791
Fragments arranged by size:
2791 2405 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2478 1163
Size: 573 742 4377
Fragments arranged by size:
4377 742 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 3142 3903 4931 1540
Size: 275 186 29 1112 761 1028 2301
Fragments arranged by size:
2301 1112 1028 761 275 186 29
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3011 3753 3838 4909 1391
Size: 399 1221 742 85 1071 2174
Fragments arranged by size:
2174 1221 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3047 844
Size: 2203 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2321 2647 4187 4361 65
Size: 1183 1073 326 1540 174 1396
Fragments arranged by size:
1540 1396 1183 1073 326 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4336 4523 1700
Size: 110 2526 187 2869
Fragments arranged by size:
2869 2526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 2540 1184
Size: 1356 4336
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 2215 1554
Size: 661 5031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 3139 4028
Size: 54 1425 50 275 18 197 1112 889
Cuts at: 4028 5657 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1112 889 275 197 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 3139 1537
Size: 275 215 1112 4090
Fragments arranged by size:
4090 1112 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2492 2516 2936 2990 3755 4352 5139
Size: 393 860 24 420 54 765 597 787
Cuts at: 5139 5407 1239
Size: 268 1524
Fragments arranged by size:
1524 860 787 765 597 420 393 268
54 24
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
EcoRV GAT'ATC
Cuts at: 2568 2779 2568
Size: 211 5481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2267 2354 2995 2267
Size: 87 641 4964
Fragments arranged by size:
4964 641 87
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2763 2795 2889 2921 4333
Size: 32 135 32 1191 32 94 32 1412
Cuts at: 4333 4365 4407 4439 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1412 1191 135 94 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2587 3036 3282 669
Size: 988 281 649 449 246 3079
Fragments arranged by size:
3079 988 649 449 281 246
HindIII A'AGCT_T
Cuts at: 748 2087 2660 748
Size: 1339 573 3780
Fragments arranged by size:
3780 1339 573
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3650 4009 4168 518
Size: 648 843 1641 359 159 2042
Fragments arranged by size:
2042 1641 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2494 2956 3108 3785 4933 5178 1606
Size: 565 323 462 152 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 462 323 245 152
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2358 2436 2720 3050 4595 1132
Size: 97 1129 78 284 330 1545 2229
Fragments arranged by size:
2229 1545 1129 330 284 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PmlI CAC'GTG
Cuts at: 2350 2350
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 2286 3262 2286
Size: 976 4716
PstI C_TGCA'G
Cuts at: 830 3044 830
Size: 2214 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2171 2494 2956 2171
Size: 323 462 4907
Fragments arranged by size:
4907 462 323
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2811 3040 4175 4856 5047 826
Size: 219 1766 229 1135 681 191 1471
Fragments arranged by size:
1766 1471 1135 681 229 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 2436 3050 1132
Size: 1304 614 3774
Fragments arranged by size:
3774 1304 614
SspI AAT'ATT
Cuts at: 5 52 2699 2850 3616 5
Size: 47 2647 151 766 2081
Fragments arranged by size:
2647 2081 766 151 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2191 2338 2440 3131 513
Size: 552 1126 147 102 691 3074
Fragments arranged by size:
3074 1126 691 552 147 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2334 2366 2628 2660 5652 5684 2334
Size: 32 262 32 2992 32 2342
Fragments arranged by size:
2992 2342 262 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2312 1316
Size: 996 4696
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 2491 2368
Size: 123 5569
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 2975 3821 2975
Size: 846 4846
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI