Restriction map for
pFN21AE3981
(Circular) (Six-base) MAPSORT of: pFN21AE3981.seq Check: 5810 from: 1 to: 5476
pFN21AE3981 5476 bp
With 182 enzymes: SgfI *
March 28, 2012 11:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2679 2819 1937
Size: 742 140 4594
Fragments arranged by size:
4594 742 140
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 3603 3976 1563
Size: 2040 373 3063
Fragments arranged by size:
3063 2040 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 3539 4923 5191 1632
Size: 707 1200 1384 268 1917
Fragments arranged by size:
1917 1384 1200 707 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 2121 4375 5352 1203
Size: 918 2254 977 1327
Fragments arranged by size:
2254 1327 977 918
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 3219 4791 3219
Size: 1572 3904
ApaLI G'TGCA_C
Cuts at: 3533 4689 3533
Size: 1156 4320
ApoI r'AATT_y
Cuts at: 1493 2269 2785 3011 3163 5272 1493
Size: 776 516 226 152 2109 1697
Fragments arranged by size:
2109 1697 776 516 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2624 2801 1870
Size: 73 681 177 4545
Fragments arranged by size:
4545 681 177 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2797 4252 618
Size: 325 217 627 1010 1455 1842
Fragments arranged by size:
1842 1455 1010 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2199 2663 2795 1391
Size: 808 464 132 4072
Fragments arranged by size:
4072 808 464 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2182 928
Size: 971 283 4222
Fragments arranged by size:
4222 971 283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 2019 1548
Size: 471 5005
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4087 136
Size: 107 122 71 631 605 2415 1525
Fragments arranged by size:
2415 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2474 2893 2474
Size: 419 5057
Bme1580I G_kGCm'C
Cuts at: 1790 3537 4693 1790
Size: 1747 1156 2573
Fragments arranged by size:
2573 1747 1156
BmgBI CAC'GTC
Cuts at: 1318 2524 2572 1318
Size: 1206 48 4222
Fragments arranged by size:
4222 1206 48
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2683 3207 4165
Size: 416 53 83 399 24 1266 524 958
Cuts at: 4165 442
Size: 1753
Fragments arranged by size:
1753 1266 958 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 4136 1239
Size: 813 2084 2579
Fragments arranged by size:
2579 2084 813
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3601 4466 4764 3601
Size: 865 298 4313
Fragments arranged by size:
4313 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4139 882
Size: 617 2640 2219
Fragments arranged by size:
2640 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3267 493
Size: 711 2063 2702
Fragments arranged by size:
2702 2063 711
BsaBI GATnn'nnATC
Cuts at: 5470 5470
Size: 5476
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2729 3283 3665 4300
Size: 53 83 186 936 1196 554 382 635
Cuts at: 4300 275
Size: 1451
Fragments arranged by size:
1451 1196 936 635 554 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2751 3909 4307 4581 4728 5139 2751
Size: 1158 398 274 147 411 3088
Fragments arranged by size:
3088 1158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5251
Fragments arranged by size:
5251 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2688 1186
Size: 1096 406 3974
Fragments arranged by size:
3974 1096 406
BseYI C'CCAG_C
Cuts at: 4679 5175 4679
Size: 496 4980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4903
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3537 3622 4693 3537
Size: 85 1071 4320
Fragments arranged by size:
4320 1071 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2663 2795 3537 3622 4693 1391
Size: 399 409 464 132 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 464 409 399 132 85
BspEI T'CCGG_A
Cuts at: 2751 5139 2751
Size: 2388 3088
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 844
Size: 1987 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3363 1394
Size: 429 1540 3507
Fragments arranged by size:
3507 1540 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 2124 96
Size: 2028 3448
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 4280 4280
Size: 5476
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 2206 2368 2137
Size: 69 162 5245
Fragments arranged by size:
5245 162 69
BstYI r'GATC_y
Cuts at: 1972 2027 2806 3173 3558 3575 5027 5113 5125
Size: 55 779 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 779 385 367 346 86 55
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4924
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2780 2780
Size: 5476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2116 2139 2923
Size: 54 1425 50 275 18 286 23 784
Cuts at: 2923 3812 5441 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 784 286 275 54 50
43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 3406 1353
Size: 975 1078 3423
Fragments arranged by size:
3423 1078 975
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2740 4063 4437 4583 387
Size: 1064 138 1151 1323 374 146 1280
Fragments arranged by size:
1323 1280 1151 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2339 3539 4136 4923 5191 1239
Size: 393 420 287 1200 597 787 268 1524
Fragments arranged by size:
1524 1200 787 597 420 393 287 268
EcoO109I rG'GnC_Cy
Cuts at: 2418 2620 2418
Size: 202 5274
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 4623 1678
Size: 2945 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2004 2036 2451 2483 2637
Size: 32 135 32 432 32 415 32 154
Cuts at: 2637 2669 4117 4149 4191 4223 1373
Size: 32 1448 32 42 32 2626
Fragments arranged by size:
2626 1448 432 415 154 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 3075 3075
Size: 5476
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2349 4590 4774 1305
Size: 1044 2241 184 2007
Fragments arranged by size:
2241 2007 1044 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 10
Size: 54 1425 343 309 3345
Fragments arranged by size:
3345 1425 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 2108 3434 3793 3952 518
Size: 648 940 2 1326 359 159 2042
Fragments arranged by size:
2042 1326 940 648 359 159 2
MspA1I CmG'CkG
Cuts at: 1606 2892 3569 4717 4962 1606
Size: 1286 677 1148 245 2120
Fragments arranged by size:
2120 1286 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4924
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2125 2834 4379 1132
Size: 97 896 709 1545 2229
Fragments arranged by size:
2229 1545 896 709 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 2121 4375 2121
Size: 2254 3222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2591 1108
Size: 1483 3993
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2620 2620
Size: 5476
PshAI GACnn'nnGTC
Cuts at: 1613 2570 1613
Size: 957 4519
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2828 830
Size: 1998 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2824 3959 4640 4831 826
Size: 219 1779 1135 681 191 1471
Fragments arranged by size:
1779 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 3580 4481 4743 820
Size: 197 926 681 956 901 262 1553
Fragments arranged by size:
1553 956 926 901 681 262 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 2345 3400 5
Size: 47 2293 1055 2081
Fragments arranged by size:
2293 2081 1055 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5476
StyI C'CwwG_G
Cuts at: 513 1065 2053 2915 513
Size: 552 988 862 3074
Fragments arranged by size:
3074 988 862 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 3722 5434
Size: 274 80 33 51 1443 147 1598 1712
Cuts at: 5434 96
Size: 138
Fragments arranged by size:
1712 1598 1443 274 147 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2719 2751 5436 5468 2719
Size: 32 2685 32 2727
Fragments arranged by size:
2727 2685 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 2624 1943
Size: 681 4795
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 2505 3605 2505
Size: 1100 4376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI