Restriction map for
pFN21AE3980
(Circular) (Six-base) MAPSORT of: pFN21AE3980.seq Check: 2297 from: 1 to: 6292
pFN21AE3980 6292 bp
With 182 enzymes: SgfI *
March 28, 2012 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3186 3434 3635 1937
Size: 1249 248 201 4594
Fragments arranged by size:
4594 1249 248 201
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4355 5739 6007 1632
Size: 2723 1384 268 1917
Fragments arranged by size:
2723 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 3285 5191 6168 1203
Size: 2082 1906 977 1327
Fragments arranged by size:
2082 1906 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 5021 5021
Size: 6292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2091 3125 1184
Size: 907 1034 4351
Fragments arranged by size:
4351 1034 907
AlwNI CAG_nnn'CTG
Cuts at: 3097 3200 4035 5607 3097
Size: 103 835 1572 3782
Fragments arranged by size:
3782 1572 835 103
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 3601 3827 3979 6088 1493
Size: 2108 226 152 2109 1697
Fragments arranged by size:
2109 2108 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3509 3617 1870
Size: 73 1566 108 4545
Fragments arranged by size:
4545 1566 108 73
BamHI G'GATC_C
Cuts at: 3622 3989 3622
Size: 367 5925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3613 5068 618
Size: 325 217 627 248 1578 1455 1842
Fragments arranged by size:
1842 1578 1455 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2513 2747 2774 2838 3323 3611 1391
Size: 1122 234 27 64 485 288 4072
Fragments arranged by size:
4072 1122 485 288 234 64 27
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 6292
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2694 928
Size: 971 795 4526
Fragments arranged by size:
4526 971 795
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2518 2552 4483 4517 1903
Size: 34 581 34 1931 34 3678
Fragments arranged by size:
3678 1931 581 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 2136 1548
Size: 588 5704
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2399 4903 136
Size: 107 122 71 631 605 727 2504 1525
Fragments arranged by size:
2504 1525 727 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 2343 2942 3369 4353 5509 1790
Size: 553 599 427 984 1156 2573
Fragments arranged by size:
2573 1156 984 599 553 427
BmgBI CAC'GTC
Cuts at: 1318 3442 1318
Size: 2124 4168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2590 2645 4023
Size: 416 53 83 399 24 1173 55 1378
Cuts at: 4023 4981 442
Size: 958 1753
Fragments arranged by size:
1753 1378 1173 958 416 399 83 55
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2603 3160 4952 1239
Size: 1139 225 557 1792 2579
Fragments arranged by size:
2579 1792 1139 557 225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4417 5282 5580 4417
Size: 865 298 5129
Fragments arranged by size:
5129 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 2579 3523 4955 882
Size: 617 703 377 944 1432 2219
Fragments arranged by size:
2219 1432 944 703 617 377
BsaAI yAC'GTr
Cuts at: 493 1204 4083 493
Size: 711 2879 2702
Fragments arranged by size:
2879 2702 711
BsaBI GATnn'nnATC
Cuts at: 2141 6286 2141
Size: 4145 2147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 2286 4099 4481
Size: 53 83 186 936 503 250 1813 382
Cuts at: 4481 5116 275
Size: 635 1451
Fragments arranged by size:
1813 1451 936 635 503 382 250 186
83 53
BsaWI w'CCGG_w
Cuts at: 2191 2880 4725 5123 5397 5544 5955 2191
Size: 689 1845 398 274 147 411 2528
Fragments arranged by size:
2528 1845 689 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2795 2825 1178
Size: 30 165 30 1392 30 4645
Fragments arranged by size:
4645 1392 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2693 2737 2740 2997 3434 1186
Size: 1189 318 44 3 257 437 4044
Fragments arranged by size:
4044 1189 437 318 257 44 3
BseYI C'CCAG_C
Cuts at: 2276 2380 2463 3130 3224 3338 3533 5495 5991
Size: 104 83 667 94 114 195 1962 496
Cuts at: 5991 2276
Size: 2577
Fragments arranged by size:
2577 1962 667 496 195 114 104 94
83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2972 1163
Size: 573 1236 4483
Fragments arranged by size:
4483 1236 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2266 3742 4503 5531 1540
Size: 275 186 265 1476 761 1028 2301
Fragments arranged by size:
2301 1476 1028 761 275 265 186
BsiHKAI G_wGCw'C
Cuts at: 2513 2838 4353 4438 5509 2513
Size: 325 1515 85 1071 3296
Fragments arranged by size:
3296 1515 1071 325 85
BsmI GAATG_Cn'
Cuts at: 3582 3803 3896 3582
Size: 221 93 5978
Fragments arranged by size:
5978 221 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2545 2798 3382 3401 2545
Size: 253 584 19 5436
Fragments arranged by size:
5436 584 253 19
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2343 2513 2747 2774 2838 2942 3323
Size: 399 553 170 234 27 64 104 381
Cuts at: 3323 3369 3611 4353 4438 5509 1391
Size: 46 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 553 399 381 242 234
170 104 85 64 46 27
BspEI T'CCGG_A
Cuts at: 2191 2880 5955 2191
Size: 689 3075 2528
Fragments arranged by size:
3075 2528 689
BspHI T'CATG_A
Cuts at: 2889 4181 5911 2889
Size: 1292 1730 3270
Fragments arranged by size:
3270 1730 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3647 844
Size: 2803 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 4179 1394
Size: 429 305 2051 3507
Fragments arranged by size:
3507 2051 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 3351 4787 4961 65
Size: 1183 2103 1436 174 1396
Fragments arranged by size:
2103 1436 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 2217 2271 2329 2518 2869 4936
Size: 110 286 121 54 58 189 351 2067
Cuts at: 4936 5123 1700
Size: 187 2869
Fragments arranged by size:
2869 2067 351 286 189 187 121 110
58 54
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6292
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6292
BstXI CCAn_nnnn'nTGG
Cuts at: 3016 3301 3016
Size: 285 6007
BstYI r'GATC_y
Cuts at: 1972 2364 3093 3622 3989 4374 4391 5843 5929
Size: 392 729 529 367 385 17 1452 86
Cuts at: 5929 5941 6287 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 729 529 392 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3435 3435
Size: 6292
Bsu36I CC'TnA_GG
Cuts at: 2243 3470 2243
Size: 1227 5065
BtgI C'CryG_G
Cuts at: 513 1065 2050 3267 513
Size: 552 985 1217 3538
Fragments arranged by size:
3538 1217 985 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2515 3319 487
Size: 683 56 1289 804 3460
Fragments arranged by size:
3460 1289 804 683 56
BtsI GCAGTG_nn'
Cuts at: 3258 3808 4602 4622 3258
Size: 550 794 20 4928
Fragments arranged by size:
4928 794 550 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3428 3463 529
Size: 35 2864 35 3358
Fragments arranged by size:
3358 2864 35 35
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2520 2989
Size: 54 1425 50 275 18 385 305 469
Cuts at: 2989 3018 3739 4628 6257 8
Size: 29 721 889 1629 43
Fragments arranged by size:
1629 1425 889 721 469 385 305 275
54 50 43 29 18
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2670 3158 3176 4222 1353
Size: 1317 488 18 1046 3423
Fragments arranged by size:
3423 1317 1046 488 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2842 3026 4879 5253 5399 387
Size: 1064 138 1253 184 1853 374 146 1280
Fragments arranged by size:
1853 1280 1253 1064 374 184 146 138
EcoICRI GAG'CTC
Cuts at: 2511 2836 2511
Size: 325 5967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2603 3160 4355 4952 5739 6007
Size: 393 746 225 557 1195 597 787 268
Cuts at: 6007 1239
Size: 1524
Fragments arranged by size:
1524 1195 787 746 597 557 393 268
225
EcoNI CCTnn'n_nnAGG
Cuts at: 3121 3121
Size: 6292
EcoO109I rG'GnC_Cy
Cuts at: 2392 3343 2392
Size: 951 5341
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2890 3520 2890
Size: 630 5662
FseI GG_CCGG'CC
Cuts at: 2522 2522
Size: 6292
FspI TGC'GCA
Cuts at: 2174 4798 2174
Size: 2624 3668
HaeII r_GCGC'y
Cuts at: 1678 2039 2479 5439 1678
Size: 361 440 2960 2531
Fragments arranged by size:
2960 2531 440 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4933 4965 5007 5039 1373
Size: 32 135 32 3361 32 42 32 2626
Fragments arranged by size:
3361 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 3636 3882 669
Size: 988 281 397 1301 246 3079
Fragments arranged by size:
3079 1301 988 397 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 2168 3891 2168
Size: 1723 4569
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3405 5406 5590 1305
Size: 2100 2001 184 2007
Fragments arranged by size:
2100 2007 2001 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3020 10
Size: 54 1425 343 1188 3282
Fragments arranged by size:
3282 1425 1188 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3299 4250 4609 4768 518
Size: 648 2133 951 359 159 2042
Fragments arranged by size:
2133 2042 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2380 2455 3708 4385 5533 5778 1606
Size: 774 75 1253 677 1148 245 2120
Fragments arranged by size:
2120 1253 1148 774 677 245 75
NaeI GCC'GGC
Cuts at: 2098 2219 2520 2871 2098
Size: 121 301 351 5519
Fragments arranged by size:
5519 351 301 121
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5740
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NgoMIV G'CCGG_C
Cuts at: 2096 2217 2518 2869 2096
Size: 121 301 351 5519
Fragments arranged by size:
5519 351 301 121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3289 3650 5195 1132
Size: 97 2060 361 1545 2229
Fragments arranged by size:
2229 2060 1545 361 97
PasI CC'CwG_GG
Cuts at: 1348 2723 2935 1348
Size: 1375 212 4705
Fragments arranged by size:
4705 1375 212
PciI A'CATG_T
Cuts at: 3285 5191 3285
Size: 1906 4386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2978 1108
Size: 1870 4422
PfoI T'CCnGG_A
Cuts at: 2088 2355 2406 3231 2088
Size: 267 51 825 5149
Fragments arranged by size:
5149 825 267 51
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3343 3343
Size: 6292
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PstI C_TGCA'G
Cuts at: 830 3093 3644 830
Size: 2263 551 3478
Fragments arranged by size:
3478 2263 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2380 2380
Size: 6292
SacI G_AGCT'C
Cuts at: 2513 2838 2513
Size: 325 5967
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 2255 2761 4540 6252 2255
Size: 506 1779 1712 2295
Fragments arranged by size:
2295 1779 1712 506
SfcI C'TryA_G
Cuts at: 826 1045 2116 3089 3640 4775 5456 5647 826
Size: 219 1071 973 551 1135 681 191 1471
Fragments arranged by size:
1471 1135 1071 973 681 551 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2399 2399
Size: 6292
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 6292
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3619 1872
Size: 1747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4396 5297 5559 820
Size: 197 926 2453 901 262 1553
Fragments arranged by size:
2453 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 3116 1517
Size: 1599 4693
StyI C'CwwG_G
Cuts at: 513 1065 3081 3731 513
Size: 552 2016 650 3074
Fragments arranged by size:
3074 2016 650 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4319 4336 4489 4674 5119 325
Size: 1255 2739 17 153 185 445 1498
Fragments arranged by size:
2739 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2759 4538
Size: 274 80 33 51 1443 276 506 1779
Cuts at: 4538 6250 96
Size: 1712 138
Fragments arranged by size:
1779 1712 1443 506 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 3300 3332 6252 6284 2795
Size: 32 473 32 2920 32 2803
Fragments arranged by size:
2920 2803 473 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3127 3446 3127
Size: 319 5973
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3617 1870
Size: 1747 4545
XmnI GAAnn'nnTTC
Cuts at: 4421 4421
Size: 6292
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI DraIII EcoRV FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SrfI SwaI