Restriction map for
pFN21AE3979
(Circular) (Six-base) MAPSORT of: pFN21AE3979.seq Check: 1196 from: 1 to: 6013
pFN21AE3979 6013 bp
With 182 enzymes: SgfI *
March 28, 2012 11:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 2956 4140 4513 1563
Size: 1393 1184 373 3063
Fragments arranged by size:
3063 1393 1184 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2915 4076 5460 5728 1632
Size: 473 810 1161 1384 268 1917
Fragments arranged by size:
1917 1384 1161 810 473 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 2362 4742 2362
Size: 2380 3633
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 2460 2521 3158 3756 5328 2460
Size: 61 637 598 1572 3145
Fragments arranged by size:
3145 1572 637 598 61
ApaLI G'TGCA_C
Cuts at: 3018 4070 5226 3018
Size: 1052 1156 3805
Fragments arranged by size:
3805 1156 1052
ApoI r'AATT_y
Cuts at: 1493 3165 3302 3322 3548 3700 5809 1493
Size: 1672 137 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 1672 226 152 137 20
AscI GG'CGCG_CC
Cuts at: 2423 2423
Size: 6013
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2147 2327 3338 1870
Size: 73 130 74 180 1011 4545
Fragments arranged by size:
4545 1011 180 130 74 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 3334 4789 618
Size: 325 217 627 267 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2280 2496 2664 3261 3332 1391
Size: 889 216 168 597 71 4072
Fragments arranged by size:
4072 889 597 216 168 71
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6013
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2921 2980 928
Size: 971 1022 59 3961
Fragments arranged by size:
3961 1022 971 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3153 3905 5115 3153
Size: 752 1210 4051
Fragments arranged by size:
4051 1210 752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2316 2444 2590
Size: 107 122 71 631 605 644 128 146
Cuts at: 2590 4624 136
Size: 2034 1525
Fragments arranged by size:
2034 1525 644 631 605 146 128 122
107 71
BglII A'GATC_T
Cuts at: 3269 6008 3269
Size: 2739 3274
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 3022 4074 5230 1790
Size: 1232 1052 1156 2573
Fragments arranged by size:
2573 1232 1156 1052
BmgBI CAC'GTC
Cuts at: 1318 2143 2499 1318
Size: 825 356 4832
Fragments arranged by size:
4832 825 356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3744 4702
Size: 416 53 83 399 24 897 1430 958
Cuts at: 4702 442
Size: 1753
Fragments arranged by size:
1753 1430 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2529 1056
Size: 1473 4540
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2437 2486 2831 2877 4673 1239
Size: 1094 104 49 345 46 1796 2579
Fragments arranged by size:
2579 1796 1094 345 104 49 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 3245 4138 5003 5301 2887
Size: 358 893 865 298 3599
Fragments arranged by size:
3599 893 865 358 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2752 4676 882
Size: 617 1253 1924 2219
Fragments arranged by size:
2219 1924 1253 617
BsaAI yAC'GTr
Cuts at: 493 1204 2233 3804 493
Size: 711 1029 1571 2702
Fragments arranged by size:
2702 1571 1029 711
BsaBI GATnn'nnATC
Cuts at: 3141 6007 3141
Size: 2866 3147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3820 4202 4837
Size: 53 83 186 936 522 1765 382 635
Cuts at: 4837 275
Size: 1451
Fragments arranged by size:
1765 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2628 4446 4844 5118 5265 5676 2628
Size: 1818 398 274 147 411 2965
Fragments arranged by size:
2965 1818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 3168 3198 1178
Size: 30 165 30 1080 30 655 30 3993
Fragments arranged by size:
3993 1080 655 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 3189 1186
Size: 1318 685 4010
Fragments arranged by size:
4010 1318 685
BseYI C'CCAG_C
Cuts at: 2407 5216 5712 2407
Size: 2809 496 2708
Fragments arranged by size:
2809 2708 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 3263 1163
Size: 573 366 1161 3913
Fragments arranged by size:
3913 1161 573 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 2197 2359 2391 3463 4224
Size: 275 186 169 27 162 32 1072 761
Cuts at: 4224 5252 1540
Size: 1028 2301
Fragments arranged by size:
2301 1072 1028 761 275 186 169 162
32 27
BsiHKAI G_wGCw'C
Cuts at: 2148 2280 3022 3261 4074 4159 5230 2148
Size: 132 742 239 813 85 1071 2931
Fragments arranged by size:
2931 1071 813 742 239 132 85
BsmI GAATG_Cn'
Cuts at: 3081 3524 3617 3081
Size: 443 93 5477
Fragments arranged by size:
5477 443 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2280 2496 2664 3022 3261 3332
Size: 399 358 132 216 168 358 239 71
Cuts at: 3332 4074 4159 5230 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 358 239 216
168 132 85 71
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3253 3902 5632 3253
Size: 649 1730 3634
Fragments arranged by size:
3634 1730 649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 3368 844
Size: 1781 743 3489
Fragments arranged by size:
3489 1781 743
BsrBI CCG'CTC
Cuts at: 1394 1823 2130 3900 1394
Size: 429 307 1770 3507
Fragments arranged by size:
3507 1770 429 307
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2112 2196 2645 4657 4844 1700
Size: 110 302 84 449 2012 187 2869
Fragments arranged by size:
2869 2012 449 302 187 110 84
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 2319 2423 1925
Size: 394 104 5515
Fragments arranged by size:
5515 394 104
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6013
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstXI CCAn_nnnn'nTGG
Cuts at: 2855 2855
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2517 3269 3343 3710 4095 4112 5564 5650
Size: 545 752 74 367 385 17 1452 86
Cuts at: 5650 5662 6008 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 752 545 385 367 346 86
74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2242 2264 2556 3003 513
Size: 552 1177 22 292 447 3523
Fragments arranged by size:
3523 1177 552 447 292 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 3251 3529 4323 4343 2700
Size: 551 278 794 20 4370
Fragments arranged by size:
4370 794 551 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2388 2508
Size: 54 1425 50 275 18 409 149 120
Cuts at: 2508 3460 4349 5978 8
Size: 952 889 1629 43
Fragments arranged by size:
1629 1425 952 889 409 275 149 120
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2388 3460 1537
Size: 275 576 1072 4090
Fragments arranged by size:
4090 1072 576 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3943 1353
Size: 2590 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2494 2632 4600 4974 5120 387
Size: 1064 138 905 138 1968 374 146 1280
Fragments arranged by size:
1968 1280 1064 905 374 146 138 138
EcoICRI GAG'CTC
Cuts at: 2278 3259 2278
Size: 981 5032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2333 2437 2486 2831 2877 2915
Size: 393 473 228 104 49 345 46 38
Cuts at: 2915 4076 4673 5460 5728 1239
Size: 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 787 597 473 393 345 268
228 104 49 46 38
EcoO109I rG'GnC_Cy
Cuts at: 2308 2308
Size: 6013
EcoRI G'AATT_C
Cuts at: 3165 3302 3322 3700 3165
Size: 137 20 378 5478
Fragments arranged by size:
5478 378 137 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 6013
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 2058 2347 2461 5160 1678
Size: 380 289 114 2699 2531
Fragments arranged by size:
2699 2531 380 289 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2348 2380 2483
Size: 32 135 32 614 32 130 32 103
Cuts at: 2483 2515 4654 4686 4728 4760 1373
Size: 32 2139 32 42 32 2626
Fragments arranged by size:
2626 2139 614 135 130 103 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2906 748
Size: 2158 3855
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 3087 5127 5311 1305
Size: 1086 696 2040 184 2007
Fragments arranged by size:
2040 2007 1086 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2561 2807 3971 4330 4489 518
Size: 648 1395 246 1164 359 159 2042
Fragments arranged by size:
2042 1395 1164 648 359 246 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2098 2266 3429 4106 5254 5499 1606
Size: 416 76 168 1163 677 1148 245 2120
Fragments arranged by size:
2120 1163 1148 677 416 245 168 76
NaeI GCC'GGC
Cuts at: 2114 2647 2114
Size: 533 5480
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6013
NcoI C'CATG_G
Cuts at: 513 1065 2556 3003 513
Size: 552 1491 447 3523
Fragments arranged by size:
3523 1491 552 447
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NgoMIV G'CCGG_C
Cuts at: 2112 2645 2112
Size: 533 5480
NheI G'CTAG_C
Cuts at: 1052 2525 1052
Size: 1473 4540
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 2365 2476 3371 4916 1132
Size: 97 1136 111 895 1545 2229
Fragments arranged by size:
2229 1545 1136 895 111 97
PasI CC'CwG_GG
Cuts at: 1348 2651 1348
Size: 1303 4710
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2460 1108
Size: 1352 4661
PfoI T'CCnGG_A
Cuts at: 2666 2666
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspXI vC'TCGA_Gb
Cuts at: 2147 2327 2147
Size: 180 5833
PstI C_TGCA'G
Cuts at: 830 3365 830
Size: 2535 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SacI G_AGCT'C
Cuts at: 2280 3261 2280
Size: 981 5032
SacII CC_GC'GG
Cuts at: 2267 2267
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 2186 2333 4261 5973 2186
Size: 147 1928 1712 2226
Fragments arranged by size:
2226 1928 1712 147
SfcI C'TryA_G
Cuts at: 826 1045 3120 3361 4496 5177 5368 826
Size: 219 2075 241 1135 681 191 1471
Fragments arranged by size:
2075 1471 1135 681 241 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6013
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 2075 3340 1872
Size: 203 1265 4545
Fragments arranged by size:
4545 1265 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2327 2866 3260 4117 5018
Size: 197 926 204 180 539 394 857 901
Cuts at: 5018 5280 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 857 539 394 262 204
197 180
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 2476 3371 1132
Size: 1344 895 3774
Fragments arranged by size:
3774 1344 895
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 1517
Size: 693 5320
StyI C'CwwG_G
Cuts at: 513 1065 2556 3003 3452 513
Size: 552 1491 447 449 3074
Fragments arranged by size:
3074 1491 552 449 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2887 3091 4040 4057 4210 4395 4840
Size: 1255 1307 204 949 17 153 185 445
Cuts at: 4840 325
Size: 1498
Fragments arranged by size:
1498 1307 1255 949 445 204 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2331 4259
Size: 274 80 33 51 1443 207 147 1928
Cuts at: 4259 5971 96
Size: 1712 138
Fragments arranged by size:
1928 1712 1443 274 207 147 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 2503 2461
Size: 42 5971
XhoI C'TCGA_G
Cuts at: 1943 2147 2327 1943
Size: 204 180 5629
Fragments arranged by size:
5629 204 180
XmaI C'CCGG_G
Cuts at: 1870 2073 3338 1870
Size: 203 1265 4545
Fragments arranged by size:
4545 1265 203
XmnI GAAnn'nnTTC
Cuts at: 2090 4142 2090
Size: 2052 3961
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI
PspOMI PsrI PvuII RsrII SanDI SapI SexAI SfiI
SrfI SwaI