Restriction map for
pFN21AE3978
(Circular) (Six-base) MAPSORT of: pFN21AE3978.seq Check: 7378 from: 1 to: 5881
pFN21AE3978 5881 bp
With 182 enzymes: SgfI *
March 28, 2012 11:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2045 3224 1937
Size: 108 1179 4594
Fragments arranged by size:
4594 1179 108
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2110 2769 2879 3944 5328 5596 1632
Size: 478 659 110 1065 1384 268 1917
Fragments arranged by size:
1917 1384 1065 659 478 268 110
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 2254 4610 2254
Size: 2356 3525
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 3624 5196 3624
Size: 1572 4309
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2096 2306 2312 2408 2633 3190 3416 3568
Size: 603 210 6 96 225 557 226 152
Cuts at: 3568 5677 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 603 557 226 225 210 152
96 6
AseI AT'TA_AT
Cuts at: 160 1032 2118 2979 4435 160
Size: 872 1086 861 1456 1606
Fragments arranged by size:
1606 1456 1086 872 861
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3206 1870
Size: 73 67 1196 4545
Fragments arranged by size:
4545 1196 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2907 2940 2907
Size: 33 5848
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 2545 3202 4657 618
Size: 325 217 627 418 340 657 1455 1842
Fragments arranged by size:
1842 1455 657 627 418 340 325 217
BanII G_rGCy'C
Cuts at: 1391 3200 1391
Size: 1809 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2599 2633 4072 4106 1903
Size: 34 662 34 1439 34 3678
Fragments arranged by size:
3678 1439 662 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4492 136
Size: 107 122 71 631 605 2820 1525
Fragments arranged by size:
2820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 3942 5098 1790
Size: 2152 1156 2573
Fragments arranged by size:
2573 2152 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 3612 4570
Size: 416 53 83 399 24 987 1208 958
Cuts at: 4570 442
Size: 1753
Fragments arranged by size:
1753 1208 987 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2655 4541 1239
Size: 816 600 1886 2579
Fragments arranged by size:
2579 1886 816 600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 5875 5875
Size: 5881
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5656
Fragments arranged by size:
5656 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5881
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2964 3007 3117 1163
Size: 573 1228 43 110 3927
Fragments arranged by size:
3927 1228 573 110 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2794 3124 3942 4027 5098 2794
Size: 330 818 85 1071 3577
Fragments arranged by size:
3577 1071 818 330 85
BsmI GAATG_Cn'
Cuts at: 2332 3153 3392 3485 2332
Size: 821 239 93 4728
Fragments arranged by size:
4728 821 239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2794 3124 3200 3942 4027 5098 1391
Size: 399 1004 330 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 1004 742 399 330 85 76
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3236 844
Size: 2392 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 4376 4550 65
Size: 1183 1110 2018 174 1396
Fragments arranged by size:
2018 1396 1183 1110 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876
Size: 1239 367 385 17 1452 86 12 346
Cuts at: 5876 1972
Size: 1977
Fragments arranged by size:
1977 1452 1239 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2210 2210
Size: 5881
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3397 4191 4211 2995
Size: 402 794 20 4665
Fragments arranged by size:
4665 794 402 20
ClaI AT'CG_AT
Cuts at: 1554 2374 1554
Size: 820 5061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 2404 2638 2758 3185 2404
Size: 234 120 427 5100
Fragments arranged by size:
5100 427 234 120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846
Size: 54 1425 50 275 18 1498 889 1629
Cuts at: 5846 8
Size: 43
Fragments arranged by size:
1629 1498 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3811 1353
Size: 2458 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2110 2655 2769 2879 3944 4541
Size: 393 423 55 545 114 110 1065 597
Cuts at: 4541 5328 5596 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1065 787 597 545 423 393 268
114 110 55
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 3073 3105 4522
Size: 32 135 32 668 32 801 32 1417
Cuts at: 4522 4554 4596 4628 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 801 668 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2465 2877 748
Size: 1717 412 3752
Fragments arranged by size:
3752 1717 412
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3067 3839 4198 4357 518
Size: 648 1901 772 359 159 2042
Fragments arranged by size:
2042 1901 772 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2280 2831 3297 3974 5122 5367 1606
Size: 674 551 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 674 551 466 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5329
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 3239 4784 1132
Size: 97 2010 1545 2229
Fragments arranged by size:
2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3149 1108
Size: 2041 3840
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PstI C_TGCA'G
Cuts at: 830 2612 2836 3233 830
Size: 1782 224 397 3478
Fragments arranged by size:
3478 1782 397 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2280 2831 2280
Size: 551 5330
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2608 2832 3229 4364 5045 5236 826
Size: 219 1563 224 397 1135 681 191 1471
Fragments arranged by size:
1563 1471 1135 681 397 224 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3985 4886 5148 820
Size: 197 926 2042 901 262 1553
Fragments arranged by size:
2042 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 3320 513
Size: 552 2255 3074
Fragments arranged by size:
3074 2255 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 2997 3908 3925 4078 4263 4708
Size: 1255 558 859 911 17 153 185 445
Cuts at: 4708 325
Size: 1498
Fragments arranged by size:
1498 1255 911 859 558 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2992 4127 5839
Size: 274 80 33 51 1443 1015 1135 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
1712 1443 1135 1015 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 3114 4010 3114
Size: 896 4985
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI