Restriction map for
pFN21AE3977
(Circular) (Six-base) MAPSORT of: pFN21AE3977.seq Check: 1946 from: 1 to: 5479
pFN21AE3977 5479 bp
With 182 enzymes: SgfI *
March 28, 2012 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2480 2480
Size: 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3289 278
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2822 1937
Size: 885 4594
Acc65I G'GTAC_C
Cuts at: 2800 2800
Size: 5479
AclI AA'CG_TT
Cuts at: 1563 3606 3979 1563
Size: 2043 373 3063
Fragments arranged by size:
3063 2043 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2796 3542 4926 5194 1632
Size: 1164 746 1384 268 1917
Fragments arranged by size:
1917 1384 1164 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5282
AflIII A'CryG_T
Cuts at: 1203 4378 5355 1203
Size: 3175 977 1327
Fragments arranged by size:
3175 1327 977
AgeI A'CCGG_T
Cuts at: 4310 4310
Size: 5479
AhdI GACnn_n'nnGTC
Cuts at: 4208 4208
Size: 5479
AleI CACnn'nnGTG
Cuts at: 1166 2055 2555 1166
Size: 889 500 4090
Fragments arranged by size:
4090 889 500
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2073 1184
Size: 889 4590
AlwNI CAG_nnn'CTG
Cuts at: 3222 4794 3222
Size: 1572 3907
ApaLI G'TGCA_C
Cuts at: 2252 3536 4692 2252
Size: 1284 1156 3039
Fragments arranged by size:
3039 1284 1156
ApoI r'AATT_y
Cuts at: 1493 2531 2788 3014 3166 5275 1493
Size: 1038 257 226 152 2109 1697
Fragments arranged by size:
2109 1697 1038 257 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4033 160
Size: 872 3001 1606
Fragments arranged by size:
3001 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 2804 1870
Size: 73 618 243 4545
Fragments arranged by size:
4545 618 243 73
BamHI G'GATC_C
Cuts at: 2809 3176 2809
Size: 367 5112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 2721 2800 4255 618
Size: 325 217 627 455 479 79 1455 1842
Fragments arranged by size:
1842 1455 627 479 455 325 217 79
BanII G_rGCy'C
Cuts at: 1391 2798 1391
Size: 1407 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 928
Size: 971 567 3941
Fragments arranged by size:
3941 971 567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2305 2339 3670 3704 1903
Size: 34 368 34 1331 34 3678
Fragments arranged by size:
3678 1331 368 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3371 4581 3371
Size: 1210 4269
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2228 2632 4090
Size: 107 122 71 631 605 556 404 1458
Cuts at: 4090 136
Size: 1525
Fragments arranged by size:
1525 1458 631 605 556 404 122 107
71
BglII A'GATC_T
Cuts at: 2175 5474 2175
Size: 3299 2180
BlpI GC'TnA_GC
Cuts at: 2709 2896 2709
Size: 187 5292
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2256 2562 3540 4696 1790
Size: 235 231 306 978 1156 2573
Fragments arranged by size:
2573 1156 978 306 235 231
BmgBI CAC'GTC
Cuts at: 1318 2557 1318
Size: 1239 4240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 4168 442
Size: 416 53 83 399 24 1793 958 1753
Fragments arranged by size:
1793 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 4139 1239
Size: 1019 1881 2579
Fragments arranged by size:
2579 1881 1019
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 3604 4469 4767 2144
Size: 1460 865 298 2856
Fragments arranged by size:
2856 1460 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4142 882
Size: 617 2643 2219
Fragments arranged by size:
2643 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3270 493
Size: 711 2066 2702
Fragments arranged by size:
2702 2066 711
BsaBI GATnn'nnATC
Cuts at: 2519 5473 2519
Size: 2954 2525
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3286 3668 4303 275
Size: 53 83 186 936 1753 382 635 1451
Fragments arranged by size:
1753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3912 4310 4584 4731 5142 3912
Size: 398 274 147 411 4249
Fragments arranged by size:
4249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 1178
Size: 30 165 30 1032 30 4192
Fragments arranged by size:
4192 1032 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5479
BseYI C'CCAG_C
Cuts at: 2669 4682 5178 2669
Size: 2013 496 2970
Fragments arranged by size:
2970 2013 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2349 1163
Size: 573 613 4293
Fragments arranged by size:
4293 613 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3690 4718 1540
Size: 275 186 928 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2773 3540 3625 4696 2256
Size: 517 767 85 1071 3039
Fragments arranged by size:
3039 1071 767 517 85
BsmI GAATG_Cn'
Cuts at: 2085 2990 3083 2085
Size: 905 93 4481
Fragments arranged by size:
4481 905 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2256 2562 2773 2798 3540 3625
Size: 399 235 231 306 211 25 742 85
Cuts at: 3625 4696 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 235 231 211
85 25
BspEI T'CCGG_A
Cuts at: 5142 5142
Size: 5479
BspHI T'CATG_A
Cuts at: 3368 5098 3368
Size: 1730 3749
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2480 2834 844
Size: 1636 354 3489
Fragments arranged by size:
3489 1636 354
BsrBI CCG'CTC
Cuts at: 1394 1823 2128 2566 3366 1394
Size: 429 305 438 800 3507
Fragments arranged by size:
3507 800 438 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 3974 4148 65
Size: 1183 2726 174 1396
Fragments arranged by size:
2726 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 2267 4123 4310 1700
Size: 110 293 164 1856 187 2869
Fragments arranged by size:
2869 1856 293 187 164 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5479
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5479
BssSI C'ACGA_G
Cuts at: 2051 3539 4551 2051
Size: 1488 1012 2979
Fragments arranged by size:
2979 1488 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5479
BstBI TT'CG_AA
Cuts at: 4283 4283
Size: 5479
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5479
BstYI r'GATC_y
Cuts at: 1972 2175 2809 3176 3561 3578 5030 5116 5128
Size: 203 634 367 385 17 1452 86 12
Cuts at: 5128 5474 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 634 385 367 346 203 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2257 2283 513
Size: 552 939 253 26 3709
Fragments arranged by size:
3709 939 552 253 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4740
Fragments arranged by size:
4740 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 2995 3789 3809 2160
Size: 835 794 20 3830
Fragments arranged by size:
3830 835 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5444
DraI TTT'AAA
Cuts at: 2783 2783
Size: 5479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4486 809
Size: 507 3170 1802
Fragments arranged by size:
3170 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2129 2220
Size: 54 1425 50 275 18 177 122 91
Cuts at: 2220 2294 2676 2926 3815 5444 8
Size: 74 382 250 889 1629 43
Fragments arranged by size:
1629 1425 889 382 275 250 177 122
91 74 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 1537
Size: 275 1114 4090
Fragments arranged by size:
4090 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2429 2435 2612 3409 1353
Size: 833 243 6 177 797 3423
Fragments arranged by size:
3423 833 797 243 177 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4066 4440 4586 387
Size: 1064 138 2477 374 146 1280
Fragments arranged by size:
2477 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2796 3542 4139 4926 5194 1239
Size: 393 626 538 746 597 787 268 1524
Fragments arranged by size:
1524 787 746 626 597 538 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2497 2569 2497
Size: 72 5407
EcoRI G'AATT_C
Cuts at: 2788 3166 2788
Size: 378 5101
FspI TGC'GCA
Cuts at: 2093 3985 2093
Size: 1892 3587
HaeII r_GCGC'y
Cuts at: 1678 2216 4626 1678
Size: 538 2410 2531
Fragments arranged by size:
2531 2410 538
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 4120 4152 4194
Size: 32 135 32 863 32 1653 32 42
Cuts at: 4194 4226 1373
Size: 32 2626
Fragments arranged by size:
2626 1653 863 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2823 3069 669
Size: 988 281 885 246 3079
Fragments arranged by size:
3079 988 885 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5479
HpaI GTT'AAC
Cuts at: 3069 3069
Size: 5479
KpnI G_GTAC'C
Cuts at: 2804 2804
Size: 5479
MfeI C'AATT_G
Cuts at: 3078 3078
Size: 5479
MluI A'CGCG_T
Cuts at: 5355 5355
Size: 5479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4593 4777 1305
Size: 3288 184 2007
Fragments arranged by size:
3288 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2222 2296 10
Size: 54 1425 343 177 213 74 3193
Fragments arranged by size:
3193 1425 343 213 177 74 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2555 3437 3796 3955 518
Size: 648 889 500 882 359 159 2042
Fragments arranged by size:
2042 889 882 648 500 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2716 2895 3572 4720 4965 1606
Size: 870 240 179 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 245 240 179
NaeI GCC'GGC
Cuts at: 2105 2105
Size: 5479
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3988
Fragments arranged by size:
3988 939 552
NdeI CA'TA_TG
Cuts at: 387 2385 387
Size: 1998 3481
NgoMIV G'CCGG_C
Cuts at: 2103 2103
Size: 5479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5479
NotI GC'GGCC_GC
Cuts at: 2926 2926
Size: 5479
NspI r_CATG'y
Cuts at: 1132 1229 2548 2837 4382 1132
Size: 97 1319 289 1545 2229
Fragments arranged by size:
2229 1545 1319 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5479
PciI A'CATG_T
Cuts at: 4378 4378
Size: 5479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5479
PfoI T'CCnGG_A
Cuts at: 2186 2186
Size: 5479
PmeI GTTT'AAAC
Cuts at: 2783 2783
Size: 5479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3548 3580 5061 5093 3548
Size: 32 1481 32 3934
Fragments arranged by size:
3934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2569 2569
Size: 5479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5479
PsiI TTA'TAA
Cuts at: 3049 3049
Size: 5479
PstI C_TGCA'G
Cuts at: 830 2310 2831 830
Size: 1480 521 3478
Fragments arranged by size:
3478 1480 521
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5479
PvuII CAG'CTG
Cuts at: 2476 2716 2476
Size: 240 5239
SalI G'TCGA_C
Cuts at: 1936 2821 1936
Size: 885 4594
SbfI CC_TGCA'GG
Cuts at: 2831 2831
Size: 5479
ScaI AGT'ACT
Cuts at: 3727 5439 3727
Size: 1712 3767
SexAI A'CCwGG_T
Cuts at: 2730 2730
Size: 5479
SfcI C'TryA_G
Cuts at: 826 1045 2306 2827 3962 4643 4834 826
Size: 219 1261 521 1135 681 191 1471
Fragments arranged by size:
1471 1261 1135 681 521 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5479
SgrAI Cr'CCGG_yG
Cuts at: 1700 2103 1700
Size: 403 5076
SmaI CCC'GGG
Cuts at: 1872 2806 1872
Size: 934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2123 3583 4484 4746 820
Size: 197 926 180 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197 180
SnaBI TAC'GTA
Cuts at: 493 3270 493
Size: 2777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5479
SphI G_CATG'C
Cuts at: 1132 2837 1132
Size: 1705 3774
SspI AAT'ATT
Cuts at: 5 52 3403 5
Size: 47 3351 2081
Fragments arranged by size:
3351 2081 47
StuI AGG'CCT
Cuts at: 1517 2591 1517
Size: 1074 4405
StyI C'CwwG_G
Cuts at: 513 1065 2004 2918 513
Size: 552 939 914 3074
Fragments arranged by size:
3074 939 914 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3506 3523 3676 3861 4306 325
Size: 1255 1926 17 153 185 445 1498
Fragments arranged by size:
1926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2541 3725 5437
Size: 274 80 33 51 1443 564 1184 1712
Cuts at: 5437 96
Size: 138
Fragments arranged by size:
1712 1443 1184 564 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2182 2214 5439 5471 2182
Size: 32 3225 32 2190
Fragments arranged by size:
3225 2190 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5479
XbaI T'CTAG_A
Cuts at: 2815 2815
Size: 5479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2696 2738 2696
Size: 42 5437
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5479
XmaI C'CCGG_G
Cuts at: 1870 2804 1870
Size: 934 4545
XmnI GAAnn'nnTTC
Cuts at: 3608 3608
Size: 5479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3287 276
Size: 53 83 186 936 1753 2468
Fragments arranged by size:
2468 1753 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI