Restriction map for
pFN21AE3976
(Circular) (Six-base) MAPSORT of: pFN21AE3976.seq Check: 805 from: 1 to: 5524
pFN21AE3976 5524 bp
With 182 enzymes: SgfI *
March 28, 2012 11:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2282 2867 1937
Size: 345 585 4594
Fragments arranged by size:
4594 585 345
Acc65I G'GTAC_C
Cuts at: 2845 2845
Size: 5524
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3587 4971 5239 1632
Size: 458 1497 1384 268 1917
Fragments arranged by size:
1917 1497 1384 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 2298 4423 5400 1203
Size: 1095 2125 977 1327
Fragments arranged by size:
2125 1327 1095 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 2089 2161 3267 4839 2089
Size: 72 1106 1572 2774
Fragments arranged by size:
2774 1572 1106 72
ApaLI G'TGCA_C
Cuts at: 3581 4737 3581
Size: 1156 4368
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2699 2849 1870
Size: 73 756 150 4545
Fragments arranged by size:
4545 756 150 73
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2845 4300 618
Size: 325 217 627 1058 1455 1842
Fragments arranged by size:
1842 1455 1058 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2082 2843 1391
Size: 691 761 4072
Fragments arranged by size:
4072 761 691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3416 4626 2643
Size: 773 1210 3541
Fragments arranged by size:
3541 1210 773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2110 4135 136
Size: 107 122 71 631 605 438 2025 1525
Fragments arranged by size:
2025 1525 631 605 438 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 2336 3585 4741 1790
Size: 546 1249 1156 2573
Fragments arranged by size:
2573 1249 1156 546
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2066 4184 1239
Size: 804 23 2118 2579
Fragments arranged by size:
2579 2118 804 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2786 3649 4514 4812 2600
Size: 186 863 865 298 3312
Fragments arranged by size:
3312 865 863 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4187 882
Size: 617 2688 2219
Fragments arranged by size:
2688 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2058 2299 2313 3315 493
Size: 711 854 241 14 1002 2702
Fragments arranged by size:
2702 1002 854 711 241 14
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3331 3713 4348
Size: 53 83 186 936 834 964 382 635
Cuts at: 4348 275
Size: 1451
Fragments arranged by size:
1451 964 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2239 3957 4355 4629 4776 5187 2239
Size: 1718 398 274 147 411 2576
Fragments arranged by size:
2576 1718 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5299
Fragments arranged by size:
5299 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 1186
Size: 1618 3906
BseYI C'CCAG_C
Cuts at: 2427 2488 4727 5223 2427
Size: 61 2239 496 2728
Fragments arranged by size:
2728 2239 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 2360 1163
Size: 573 543 81 4327
Fragments arranged by size:
4327 573 543 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2185 2189 2974 3735 4763 1540
Size: 275 186 184 4 785 761 1028 2301
Fragments arranged by size:
2301 1028 785 761 275 186 184 4
BsiHKAI G_wGCw'C
Cuts at: 2082 2255 3585 3670 4741 2082
Size: 173 1330 85 1071 2865
Fragments arranged by size:
2865 1330 1071 173 85
BsmI GAATG_Cn'
Cuts at: 2164 2679 3035 3128 2164
Size: 515 356 93 4560
Fragments arranged by size:
4560 515 356 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2255 2336 2843 3585 3670 4741
Size: 399 292 173 81 507 742 85 1071
Cuts at: 4741 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 507 399 292 173 85
81
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2713 3411 1394
Size: 429 890 698 3507
Fragments arranged by size:
3507 890 698 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2232 2445 2791 4168 4355 1700
Size: 110 422 213 346 1377 187 2869
Fragments arranged by size:
2869 1377 422 346 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 2399 2414 3584 4596 2399
Size: 15 1170 1012 3327
Fragments arranged by size:
3327 1170 1012 15
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5524
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5524
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2113
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2116 2667 2772 2854 3221 3606 3623 5075
Size: 144 551 105 82 367 385 17 1452
Cuts at: 5075 5161 5173 5519 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 551 385 367 346 144 105
86 82 17 12
BstZ17I GTA'TAC
Cuts at: 2283 2283
Size: 5524
BtgI C'CryG_G
Cuts at: 513 1065 2004 2287 2343 513
Size: 552 939 283 56 3694
Fragments arranged by size:
3694 939 552 283 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4785
Fragments arranged by size:
4785 683 56
BtsI GCAGTG_nn'
Cuts at: 3040 3834 3854 3040
Size: 794 20 4710
Fragments arranged by size:
4710 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2382 2417 529
Size: 35 1818 35 3636
Fragments arranged by size:
3636 1818 35 35
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DraIII CAC_nnn'GTG
Cuts at: 2344 2344
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2971 3860 5489
Size: 54 1425 50 275 18 1141 889 1629
Cuts at: 5489 8
Size: 43
Fragments arranged by size:
1629 1425 1141 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2971 1537
Size: 275 1159 4090
Fragments arranged by size:
4090 1159 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3454 1353
Size: 2101 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2080 2080
Size: 5524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2066 2090 3587 4184 4971 5239
Size: 393 411 23 24 1497 597 787 268
Cuts at: 5239 1239
Size: 1524
Fragments arranged by size:
1524 1497 787 597 411 393 268 24
23
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2819 2072
Size: 747 4777
EcoO109I rG'GnC_Cy
Cuts at: 2050 2064 2050
Size: 14 5510
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 5524
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2764 4671 1678
Size: 1086 1907 2531
Fragments arranged by size:
2531 1907 1086
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 3114 669
Size: 988 281 930 246 3079
Fragments arranged by size:
3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KpnI G_GTAC'C
Cuts at: 2849 2849
Size: 5524
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 4822 1305
Size: 3333 184 2007
Fragments arranged by size:
3333 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3702
Fragments arranged by size:
3702 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2597 3482 3841 4000 518
Size: 648 1431 885 359 159 2042
Fragments arranged by size:
2042 1431 885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2329 2707 2788 2940 3617 4765 5010
Size: 552 171 378 81 152 677 1148 245
Cuts at: 5010 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 552 378 245 171 152
81
NaeI GCC'GGC
Cuts at: 2793 2793
Size: 5524
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4033
Fragments arranged by size:
4033 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5524
NgoMIV G'CCGG_C
Cuts at: 2791 2791
Size: 5524
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2134 2882 4427 1132
Size: 97 905 748 1545 2229
Fragments arranged by size:
2229 1545 905 748 97
PasI CC'CwG_GG
Cuts at: 1348 2749 1348
Size: 1401 4123
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 1108
Size: 981 4543
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PmlI CAC'GTG
Cuts at: 2299 2313 2299
Size: 14 5510
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2050 2050
Size: 5524
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5524
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2876 830
Size: 2046 3478
PvuI CG_AT'CG
Cuts at: 2001 2189 2001
Size: 188 5336
PvuII CAG'CTG
Cuts at: 2158 2158
Size: 5524
SacI G_AGCT'C
Cuts at: 2082 2082
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SanDI GG'GwC_CC
Cuts at: 2050 2050
Size: 5524
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 3772 5484 3772
Size: 1712 3812
SexAI A'CCwGG_T
Cuts at: 2820 2820
Size: 5524
SfcI C'TryA_G
Cuts at: 826 1045 2637 2872 4007 4688 4879 826
Size: 219 1592 235 1135 681 191 1471
Fragments arranged by size:
1592 1471 1135 681 235 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2615 2801 3628 4529 4791 820
Size: 197 926 672 186 827 901 262 1553
Fragments arranged by size:
1553 926 901 827 672 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 2807 1517
Size: 1290 4234
StyI C'CwwG_G
Cuts at: 513 1065 2004 2963 513
Size: 552 939 959 3074
Fragments arranged by size:
3074 959 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2199 3551 3568 3721 3906 4351 325
Size: 1255 619 1352 17 153 185 445 1498
Fragments arranged by size:
1498 1352 1255 619 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2589 3770 5482
Size: 274 80 33 51 1443 612 1181 1712
Cuts at: 5482 96
Size: 138
Fragments arranged by size:
1712 1443 1181 612 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 3653 3653
Size: 5524
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI
SrfI SwaI XcmI