Restriction map for
pFN21AE3974
(Circular) (Six-base) MAPSORT of: pFN21AE3974.seq Check: 7839 from: 1 to: 5569
pFN21AE3974 5569 bp
With 182 enzymes: SgfI *
March 28, 2012 11:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2912 1937
Size: 975 4594
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 3632 5016 5284 1632
Size: 554 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 554 268
AfeI AGC'GCT
Cuts at: 2066 2066
Size: 5569
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2201 3312 4884 2201
Size: 1111 1572 2886
Fragments arranged by size:
2886 1572 1111
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2894 1870
Size: 73 951 4545
Fragments arranged by size:
4545 951 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2783 2816 2783
Size: 33 5536
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 2890 4345 618
Size: 325 217 627 888 215 1455 1842
Fragments arranged by size:
1842 1455 888 627 325 217 215
BanII G_rGCy'C
Cuts at: 1391 2413 2634 2888 1391
Size: 1022 221 254 4072
Fragments arranged by size:
4072 1022 254 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2225 2259 3760 3794 1903
Size: 34 288 34 1501 34 3678
Fragments arranged by size:
3678 1501 288 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5569
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4180 136
Size: 107 122 71 631 605 2508 1525
Fragments arranged by size:
2508 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2468 2737 2986 2468
Size: 269 249 5051
Fragments arranged by size:
5051 269 249
Bme1580I G_kGCm'C
Cuts at: 1790 3630 4786 1790
Size: 1840 1156 2573
Fragments arranged by size:
2573 1840 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 3300 4258
Size: 416 53 83 399 24 966 917 958
Cuts at: 4258 442
Size: 1753
Fragments arranged by size:
1753 966 958 917 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2727 2780 4229 1239
Size: 1488 53 1449 2579
Fragments arranged by size:
2579 1488 1449 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 3694 4559 4857 2516
Size: 1178 865 298 3228
Fragments arranged by size:
3228 1178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2694 4232 882
Size: 617 1195 1538 2219
Fragments arranged by size:
2219 1538 1195 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 3758 4393 275
Size: 53 83 186 936 1843 382 635 1451
Fragments arranged by size:
1843 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4002 4400 4674 4821 5232 4002
Size: 398 274 147 411 4339
Fragments arranged by size:
4339 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 2127 2157 2223
Size: 30 165 30 639 30 55 30 66
Cuts at: 2223 2253 1178
Size: 30 4494
Fragments arranged by size:
4494 639 165 66 55 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2624 1186
Size: 1438 4131
BseYI C'CCAG_C
Cuts at: 2472 4772 5268 2472
Size: 2300 496 2773
Fragments arranged by size:
2773 2300 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 1163
Size: 573 398 4598
Fragments arranged by size:
4598 573 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3019 3780 4808 1540
Size: 275 186 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 2738 3630 3715 4786 2634
Size: 104 892 85 1071 3417
Fragments arranged by size:
3417 1071 892 104 85
BsmI GAATG_Cn'
Cuts at: 2037 2248 3080 3173 2037
Size: 211 832 93 4433
Fragments arranged by size:
4433 832 211 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2413 2634 2738 2888 3630 3715 4786
Size: 399 623 221 104 150 742 85 1071
Cuts at: 4786 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 623 399 221 150 104
85
BspEI T'CCGG_A
Cuts at: 5232 5232
Size: 5569
BspHI T'CATG_A
Cuts at: 3458 5188 3458
Size: 1730 3839
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2924 844
Size: 2080 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2424 2467 3456 1394
Size: 429 601 43 989 3507
Fragments arranged by size:
3507 989 601 429 43
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4213 4400 1700
Size: 110 2403 187 2869
Fragments arranged by size:
2869 2403 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 2685 3629 4641 2685
Size: 944 1012 3613
Fragments arranged by size:
3613 1012 944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5569
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5569
BstXI CCAn_nnnn'nTGG
Cuts at: 2344 2373 2344
Size: 29 5540
BstYI r'GATC_y
Cuts at: 1972 2899 3266 3651 3668 5120 5206 5218 5564
Size: 927 367 385 17 1452 86 12 346
Cuts at: 5564 1972
Size: 1977
Fragments arranged by size:
1977 1452 927 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5017
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 2820 2828 3085 3879 3899 2820
Size: 8 257 794 20 4490
Fragments arranged by size:
4490 794 257 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2694 3016 3905
Size: 54 1425 50 275 18 864 322 889
Cuts at: 3905 5534 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 864 322 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3016 1537
Size: 275 1204 4090
Fragments arranged by size:
4090 1204 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 1353
Size: 2146 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2632 2632
Size: 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2727 2780 3632 4229 5016 5284
Size: 393 554 541 53 852 597 787 268
Cuts at: 5284 1239
Size: 1524
Fragments arranged by size:
1524 852 787 597 554 541 393 268
53
EcoO109I rG'GnC_Cy
Cuts at: 2119 2311 2427 2821 2119
Size: 192 116 394 4867
Fragments arranged by size:
4867 394 192 116
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 4075 4075
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2068 4716 1678
Size: 390 2648 2531
Fragments arranged by size:
2648 2531 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373
Size: 32 135 32 2638 32 42 32 2626
Fragments arranged by size:
2638 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2913 3159 669
Size: 988 281 975 246 3079
Fragments arranged by size:
3079 988 975 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 2079 3168 2079
Size: 1089 4480
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2629 4683 4867 1305
Size: 1324 2054 184 2007
Fragments arranged by size:
2054 2007 1324 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 10
Size: 54 1425 343 864 2883
Fragments arranged by size:
2883 1425 864 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2109 3527 3886 4045 518
Size: 648 943 1418 359 159 2042
Fragments arranged by size:
2042 1418 943 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2472 2985 3662 4810 5055 1606
Size: 866 513 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 677 513 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5017
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2927 4472 1132
Size: 97 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 1108
Size: 911 4658
PfoI T'CCnGG_A
Cuts at: 2743 2743
Size: 5569
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2311 2119
Size: 192 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2921 830
Size: 2091 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2472 2472
Size: 5569
SacI G_AGCT'C
Cuts at: 2634 2634
Size: 5569
SalI G'TCGA_C
Cuts at: 1936 2911 1936
Size: 975 4594
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2917 4052 4733 4924 826
Size: 219 1872 1135 681 191 1471
Fragments arranged by size:
1872 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2495 3673 4574 4836 820
Size: 197 926 552 1178 901 262 1553
Fragments arranged by size:
1553 1178 926 901 552 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 3008 513
Size: 552 1943 3074
Fragments arranged by size:
3074 1943 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2578 3596 3613 3766 3951 4396 325
Size: 1255 998 1018 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1018 998 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2223 2255 5529 5561 2127
Size: 32 64 32 3274 32 2135
Fragments arranged by size:
3274 2135 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2645 2572
Size: 73 5496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5569
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI