Restriction map for
pFN21AE3972
(Circular) (Six-base) MAPSORT of: pFN21AE3972.seq Check: 3217 from: 1 to: 6013
pFN21AE3972 6013 bp
With 182 enzymes: SgfI *
March 28, 2012 11:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4076 5460 5728 1632
Size: 2444 1384 268 1917
Fragments arranged by size:
2444 1917 1384 268
AfeI AGC'GCT
Cuts at: 3287 3287
Size: 6013
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 2405 2478 4912 5889 1203
Size: 1202 73 2434 977 1327
Fragments arranged by size:
2434 1327 1202 977 73
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2365 1184
Size: 1181 4832
AlwNI CAG_nnn'CTG
Cuts at: 2166 2196 3300 3756 5328 2166
Size: 30 1104 456 1572 2851
Fragments arranged by size:
2851 1572 1104 456 30
ApaLI G'TGCA_C
Cuts at: 2655 4070 5226 2655
Size: 1415 1156 3442
Fragments arranged by size:
3442 1415 1156
ApoI r'AATT_y
Cuts at: 1493 3030 3322 3548 3700 5809 1493
Size: 1537 292 226 152 2109 1697
Fragments arranged by size:
2109 1697 1537 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2954 4567 160
Size: 872 1922 1613 1606
Fragments arranged by size:
1922 1613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 3338 1870
Size: 73 1395 4545
Fragments arranged by size:
4545 1395 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2442 2739 3334 4789 618
Size: 325 217 627 655 297 595 1455 1842
Fragments arranged by size:
1842 1455 655 627 595 325 297 217
BanII G_rGCy'C
Cuts at: 1391 3332 1391
Size: 1941 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 928
Size: 971 389 4653
Fragments arranged by size:
4653 971 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 3015 1548
Size: 1467 4546
BfrBI ATG'CAT
Cuts at: 2327 3113 2327
Size: 786 5227
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2659 4074 5230 1790
Size: 869 1415 1156 2573
Fragments arranged by size:
2573 1415 1156 869
BmgBI CAC'GTC
Cuts at: 1318 2017 1318
Size: 699 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3744 4702 442
Size: 416 53 83 399 24 2327 958 1753
Fragments arranged by size:
2327 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 3018 2068
Size: 950 5063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2143 3092 3140 4673 1239
Size: 904 949 48 1533 2579
Fragments arranged by size:
2579 1533 949 904 48
Bpu10I CC'TnA_GC
Cuts at: 2151 2417 2151
Size: 266 5747
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 2627 3167 4138 5003 5301 2579
Size: 48 540 971 865 298 3291
Fragments arranged by size:
3291 971 865 540 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 2597 2723 4676 882
Size: 617 662 436 126 1953 2219
Fragments arranged by size:
2219 1953 662 617 436 126
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3820 4202 4837 275
Size: 53 83 186 936 2287 382 635 1451
Fragments arranged by size:
2287 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5788
Fragments arranged by size:
5788 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 1186
Size: 837 5176
BseYI C'CCAG_C
Cuts at: 5216 5712 5216
Size: 496 5517
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2602 1163
Size: 573 866 4574
Fragments arranged by size:
4574 866 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 2659 3126 4074 4159 5230 2152
Size: 507 467 948 85 1071 2935
Fragments arranged by size:
2935 1071 948 507 467 85
BsmI GAATG_Cn'
Cuts at: 2562 2806 3524 3617 2562
Size: 244 718 93 4958
Fragments arranged by size:
4958 718 244 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2659 3126 3332 4074 4159 5230
Size: 399 362 507 467 206 742 85 1071
Cuts at: 5230 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 507 467 399 362 206
85
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 3368 844
Size: 1184 1340 3489
Fragments arranged by size:
3489 1340 1184
BsrBI CCG'CTC
Cuts at: 1394 1823 3151 3900 1394
Size: 429 1328 749 3507
Fragments arranged by size:
3507 1328 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 2384 2462 96
Size: 2288 78 3647
Fragments arranged by size:
3647 2288 78
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2739 3300 794
Size: 1945 561 3507
Fragments arranged by size:
3507 1945 561
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 3343 3710 4095 4112 5564 5650 5662 6008
Size: 1371 367 385 17 1452 86 12 346
Cuts at: 6008 1972
Size: 1977
Fragments arranged by size:
1977 1452 1371 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2822 513
Size: 552 1757 3704
Fragments arranged by size:
3704 1757 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 2659 3529 4323 4343 2659
Size: 870 794 20 4329
Fragments arranged by size:
4329 870 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978
Size: 54 1425 50 275 18 1630 889 1629
Cuts at: 5978 8
Size: 43
Fragments arranged by size:
1630 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2919 3125 3943 1353
Size: 1566 206 818 3423
Fragments arranged by size:
3423 1566 818 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2143 3092 3140 4076 4673 5460 5728
Size: 393 511 949 48 936 597 787 268
Cuts at: 5728 1239
Size: 1524
Fragments arranged by size:
1524 949 936 787 597 511 393 268
48
EcoO109I rG'GnC_Cy
Cuts at: 2163 3051 3207 2163
Size: 888 156 4969
Fragments arranged by size:
4969 888 156
EcoRI G'AATT_C
Cuts at: 3030 3322 3700 3030
Size: 292 378 5343
Fragments arranged by size:
5343 378 292
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 3289 5160 1678
Size: 1611 1871 2531
Fragments arranged by size:
2531 1871 1611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3288 3320 4654 4686 4728
Size: 32 135 32 1716 32 1334 32 42
Cuts at: 4728 4760 1373
Size: 32 2626
Fragments arranged by size:
2626 1716 1334 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 2802 2983 5127 5311 1305
Size: 1191 306 181 2144 184 2007
Fragments arranged by size:
2144 2007 1191 306 184 181
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3971 4330 4489 518
Size: 648 2805 359 159 2042
Fragments arranged by size:
2805 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2145 3070 3429 4106 5254 5499 1606
Size: 539 925 359 677 1148 245 2120
Fragments arranged by size:
2120 1148 925 677 539 359 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5461
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NsiI A_TGCA'T
Cuts at: 2329 3115 2329
Size: 786 5227
NspI r_CATG'y
Cuts at: 1132 1229 2409 2482 3371 4916 1132
Size: 97 1180 73 889 1545 2229
Fragments arranged by size:
2229 1545 1180 889 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 2405 2478 4912 2405
Size: 73 2434 3506
Fragments arranged by size:
3506 2434 73
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2166 1108
Size: 1058 4955
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 5595 5627 4082
Size: 32 1481 32 4468
Fragments arranged by size:
4468 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3207 3207
Size: 6013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2088 2120 2088
Size: 32 5981
PstI C_TGCA'G
Cuts at: 830 2354 3296 3365 830
Size: 1524 942 69 3478
Fragments arranged by size:
3478 1524 942 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
PvuII CAG'CTG
Cuts at: 3070 3070
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SapI GCTCTTCn'nnn_
Cuts at: 3125 3125
Size: 6013
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 2367 4261 5973 2367
Size: 1894 1712 2407
Fragments arranged by size:
2407 1894 1712
SfcI C'TryA_G
Cuts at: 826 1045 2350 3292 3361 4496 5177 5368 826
Size: 219 1305 942 69 1135 681 191 1471
Fragments arranged by size:
1471 1305 1135 942 681 219 191 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 2642 3146 4117 5018 5280
Size: 197 926 651 48 504 971 901 262
Cuts at: 5280 820
Size: 1553
Fragments arranged by size:
1553 971 926 901 651 504 262 197
48
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 2572 152
Size: 2420 3593
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 2257 2835 3223 3937 5
Size: 47 2205 578 388 714 2081
Fragments arranged by size:
2205 2081 714 578 388 47
StuI AGG'CCT
Cuts at: 1517 2600 1517
Size: 1083 4930
StyI C'CwwG_G
Cuts at: 513 1065 2553 3452 513
Size: 552 1488 899 3074
Fragments arranged by size:
3074 1488 899 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2370 4040 4057 4210 4395 4840 325
Size: 1255 790 1670 17 153 185 445 1498
Fragments arranged by size:
1670 1498 1255 790 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2365 2384 2408
Size: 274 80 33 51 1443 388 19 24
Cuts at: 2408 2462 2646 4259 5971 96
Size: 54 184 1613 1712 138
Fragments arranged by size:
1712 1613 1443 388 274 184 138 80
54 51 33 24 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 2122 3349 2122
Size: 1227 4786
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 4142 4142
Size: 6013
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI