Restriction map for
pFN21AE3971
(Circular) (Six-base) MAPSORT of: pFN21AE3971.seq Check: 1963 from: 1 to: 6067
pFN21AE3971 6067 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2551 3410 1937
Size: 614 859 4594
Fragments arranged by size:
4594 859 614
Acc65I G'GTAC_C
Cuts at: 2435 3388 2435
Size: 953 5114
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2595 3117 4130 5514 5782 1632
Size: 900 63 522 1013 1384 268 1917
Fragments arranged by size:
1917 1384 1013 900 522 268 63
AflII C'TTAA_G
Cuts at: 820 1017 2855 820
Size: 197 1838 4032
Fragments arranged by size:
4032 1838 197
AflIII A'CryG_T
Cuts at: 1203 4966 5943 1203
Size: 3763 977 1327
Fragments arranged by size:
3763 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2260 2421 1184
Size: 1076 161 4830
Fragments arranged by size:
4830 1076 161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2108 2140 2108
Size: 32 6035
AlwNI CAG_nnn'CTG
Cuts at: 3260 3810 5382 3260
Size: 550 1572 3945
Fragments arranged by size:
3945 1572 550
ApaI G_GGCC'C
Cuts at: 2048 2077 2048
Size: 29 6038
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3071 3376 3602 3754 5863 1493
Size: 1578 305 226 152 2109 1697
Fragments arranged by size:
2109 1697 1578 305 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 3392 1870
Size: 73 1449 4545
Fragments arranged by size:
4545 1449 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2419 2452 2419
Size: 33 6034
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2435 3388 4843 618
Size: 325 217 627 267 381 953 1455 1842
Fragments arranged by size:
1842 1455 953 627 381 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2048 2077 2228 3386 1391
Size: 657 29 151 1158 4072
Fragments arranged by size:
4072 1158 657 151 29
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5096
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2098 3959 5169 2098
Size: 1861 1210 2996
Fragments arranged by size:
2996 1861 1210
BclI T'GATC_A
Cuts at: 1548 2365 2797 1548
Size: 817 432 4818
Fragments arranged by size:
4818 817 432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2052 4678
Size: 107 122 71 631 605 337 43 2626
Cuts at: 4678 136
Size: 1525
Fragments arranged by size:
2626 1525 631 605 337 122 107 71
43
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2077 3290 4128 5284 1790
Size: 258 29 1213 838 1156 2573
Fragments arranged by size:
2573 1213 1156 838 258 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6067
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2251 3798 4756
Size: 416 53 83 399 24 834 1547 958
Cuts at: 4756 442
Size: 1753
Fragments arranged by size:
1753 1547 958 834 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2135 2306 2552 4727 1239
Size: 887 9 171 246 2175 2579
Fragments arranged by size:
2579 2175 887 246 171 9
Bpu10I CC'TnA_GC
Cuts at: 2222 2222
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 4192 5057 5355 2486
Size: 1706 865 298 3198
Fragments arranged by size:
3198 1706 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3273 4730 882
Size: 617 1774 1457 2219
Fragments arranged by size:
2219 1774 1457 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 2148 6061 2148
Size: 3913 2154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3874 4256 4891
Size: 53 83 186 936 522 1819 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1819 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2097 4500 4898 5172 5319 5730 2097
Size: 2403 398 274 147 411 2434
Fragments arranged by size:
2434 2403 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 2279 2309 1178
Size: 30 165 30 705 30 141 30 4936
Fragments arranged by size:
4936 705 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2840 3075 1186
Size: 1654 235 4178
Fragments arranged by size:
4178 1654 235
BseYI C'CCAG_C
Cuts at: 2026 5270 5766 2026
Size: 3244 496 2327
Fragments arranged by size:
3244 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2366 2954 3323 1163
Size: 573 630 588 369 3907
Fragments arranged by size:
3907 630 588 573 369
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2228 4128 4213 5284 2228
Size: 1900 85 1071 3011
Fragments arranged by size:
3011 1900 1071 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2077 2228 3290 3386 4128 4213
Size: 399 258 29 151 1062 96 742 85
Cuts at: 4213 5284 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1062 742 399 258 151 96
85 29
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 2677 2866 3956 5686 2677
Size: 189 1090 1730 3058
Fragments arranged by size:
3058 1730 1090 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2680 2956 3422 844
Size: 1836 276 466 3489
Fragments arranged by size:
3489 1836 466 276
BsrBI CCG'CTC
Cuts at: 1394 1823 3954 1394
Size: 429 2131 3507
Fragments arranged by size:
3507 2131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 3185 1184
Size: 2001 4066
BstXI CCAn_nnnn'nTGG
Cuts at: 2608 2828 2608
Size: 220 5847
BstYI r'GATC_y
Cuts at: 1972 2149 2302 2931 3397 3764 4149 4166 5618
Size: 177 153 629 466 367 385 17 1452
Cuts at: 5618 5704 5716 6062 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 629 466 385 367 346 177
153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3011 3011
Size: 6067
BtgI C'CryG_G
Cuts at: 513 1065 2067 513
Size: 552 1002 4513
Fragments arranged by size:
4513 1002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3288 3583 4377 4397 3288
Size: 295 794 20 4958
Fragments arranged by size:
4958 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 2169 1554
Size: 615 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032
Size: 54 1425 50 275 18 1684 889 1629
Cuts at: 6032 8
Size: 43
Fragments arranged by size:
1684 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3997 1353
Size: 2644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2226 2226
Size: 6067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2135 2306 2532 2552 2595 3117
Size: 393 494 9 171 226 20 43 522
Cuts at: 3117 4130 4727 5514 5782 1239
Size: 1013 597 787 268 1524
Fragments arranged by size:
1524 1013 787 597 522 494 393 268
226 171 43 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 2855
Size: 6067
EcoO109I rG'GnC_Cy
Cuts at: 2044 2073 2074 2669 2044
Size: 29 1 595 5442
Fragments arranged by size:
5442 595 29 1
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2519 2519
Size: 6067
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2058 5214 1678
Size: 380 3156 2531
Fragments arranged by size:
3156 2531 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 3130 3162 4708
Size: 32 135 32 722 32 804 32 1546
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1546 804 722 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2998 3007 3411 3657 669
Size: 988 281 1060 9 404 246 3079
Fragments arranged by size:
3079 1060 988 404 281 246 9
HindIII A'AGCT_T
Cuts at: 748 2133 2684 748
Size: 1385 551 4131
Fragments arranged by size:
4131 1385 551
HpaI GTT'AAC
Cuts at: 3007 3657 3007
Size: 650 5417
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6067
KpnI G_GTAC'C
Cuts at: 2439 3392 2439
Size: 953 5114
MfeI C'AATT_G
Cuts at: 2645 3666 2645
Size: 1021 5046
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2659 5181 5365 1305
Size: 1354 2522 184 2007
Fragments arranged by size:
2522 2007 1354 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3273 4025 4384 4543 518
Size: 648 2107 752 359 159 2042
Fragments arranged by size:
2107 2042 752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2380 2395 3483 4160 5308 5553 1606
Size: 774 15 1088 677 1148 245 2120
Fragments arranged by size:
2120 1148 1088 774 677 245 15
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5515
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 3425 4970 1132
Size: 97 2196 1545 2229
Fragments arranged by size:
2229 2196 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2048 1348
Size: 700 5367
PciI A'CATG_T
Cuts at: 4966 4966
Size: 6067
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 1108
Size: 1176 4891
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2140 4136 4168 5649 5681 2108
Size: 32 1996 32 1481 32 2494
Fragments arranged by size:
2494 1996 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2669 2669
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 2044 2073 2044
Size: 29 6038
PstI C_TGCA'G
Cuts at: 830 2400 2750 3419 830
Size: 1570 350 669 3478
Fragments arranged by size:
3478 1570 669 350
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2380 2395 2380
Size: 15 6052
SacI G_AGCT'C
Cuts at: 2228 2228
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 2606 4315 6027 2606
Size: 1709 1712 2646
Fragments arranged by size:
2646 1712 1709
SfcI C'TryA_G
Cuts at: 826 1045 2396 2746 3415 4550 5231 5422 826
Size: 219 1351 350 669 1135 681 191 1471
Fragments arranged by size:
1471 1351 1135 681 669 350 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 2855 4171 5072 5334 820
Size: 197 926 522 390 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 522 390 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2874 1517
Size: 1357 4710
StyI C'CwwG_G
Cuts at: 513 1065 2821 2875 3506 513
Size: 552 1756 54 631 3074
Fragments arranged by size:
3074 1756 631 552 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2359 2604 4313
Size: 274 80 33 51 1443 382 245 1709
Cuts at: 4313 6025 96
Size: 1712 138
Fragments arranged by size:
1712 1709 1443 382 274 245 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2123 2544 4196 2123
Size: 421 1652 3994
Fragments arranged by size:
3994 1652 421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI