Restriction map for
pFN21AE3968
(Circular) (Six-base) MAPSORT of: pFN21AE3968.seq Check: 4665 from: 1 to: 5920
pFN21AE3968 5920 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3730 278
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 3263 1937
Size: 850 476 4594
Fragments arranged by size:
4594 850 476
Acc65I G'GTAC_C
Cuts at: 3241 3241
Size: 5920
AclI AA'CG_TT
Cuts at: 1563 4047 4420 1563
Size: 2484 373 3063
Fragments arranged by size:
3063 2484 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2533 2855 2861 3150 3983 5367 5635 1632
Size: 901 322 6 289 833 1384 268 1917
Fragments arranged by size:
1917 1384 901 833 322 289 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5723
AflIII A'CryG_T
Cuts at: 1203 4819 5796 1203
Size: 3616 977 1327
Fragments arranged by size:
3616 1327 977
AgeI A'CCGG_T
Cuts at: 4751 4751
Size: 5920
AhdI GACnn_n'nnGTC
Cuts at: 4649 4649
Size: 5920
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2330 1184
Size: 1146 4774
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 5888
AlwNI CAG_nnn'CTG
Cuts at: 2556 3160 3663 5235 2556
Size: 604 503 1572 3241
Fragments arranged by size:
3241 1572 604 503
ApaLI G'TGCA_C
Cuts at: 3977 5133 3977
Size: 1156 4764
ApoI r'AATT_y
Cuts at: 1493 3229 3455 3607 5716 1493
Size: 1736 226 152 2109 1697
Fragments arranged by size:
2109 1736 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4474 160
Size: 872 3442 1606
Fragments arranged by size:
3442 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
AvaI C'yCGr_G
Cuts at: 1870 1943 2367 2702 3245 1870
Size: 73 424 335 543 4545
Fragments arranged by size:
4545 543 424 335 73
BamHI G'GATC_C
Cuts at: 2388 3250 3617 2388
Size: 862 367 4691
Fragments arranged by size:
4691 862 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3068 3241 4696 618
Size: 325 217 627 1281 173 1455 1842
Fragments arranged by size:
1842 1455 1281 627 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2154 3012 3146 3189 3239 1391
Size: 763 858 134 43 50 4072
Fragments arranged by size:
4072 858 763 134 50 43
BbeI G_GCGC'C
Cuts at: 3072 3072
Size: 5920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2591 928
Size: 971 692 4257
Fragments arranged by size:
4257 971 692
BbvCI CC'TCA_GC
Cuts at: 2210 2210
Size: 5920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4111 4145 1903
Size: 34 2174 34 3678
Fragments arranged by size:
3678 2174 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3812 5022 3812
Size: 1210 4710
BclI T'GATC_A
Cuts at: 1548 2097 1548
Size: 549 5371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2990 4531 136
Size: 107 122 71 631 605 1318 1541 1525
Fragments arranged by size:
1541 1525 1318 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5915 5915
Size: 5920
BlpI GC'TnA_GC
Cuts at: 3337 3337
Size: 5920
Bme1580I G_kGCm'C
Cuts at: 1790 2236 2319 3173 3981 5137 1790
Size: 446 83 854 808 1156 2573
Fragments arranged by size:
2573 1156 854 808 446 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5920
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2967 3053 3651
Size: 416 53 83 399 24 1550 86 598
Cuts at: 3651 4609 442
Size: 958 1753
Fragments arranged by size:
1753 1550 958 598 416 399 86 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5920
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 5920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2499 2828 2870 2903 2960 4580 1239
Size: 914 346 329 42 33 57 1620 2579
Fragments arranged by size:
2579 1620 914 346 329 57 42 33
Bpu10I CC'TnA_GC
Cuts at: 2210 2210
Size: 5920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4045 4910 5208 4045
Size: 865 298 4757
Fragments arranged by size:
4757 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4583 882
Size: 617 3084 2219
Fragments arranged by size:
3084 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3711 493
Size: 711 2507 2702
Fragments arranged by size:
2702 2507 711
BsaBI GATnn'nnATC
Cuts at: 5914 5914
Size: 5920
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3069 3727 4109 4744
Size: 53 83 186 936 1536 658 382 635
Cuts at: 4744 275
Size: 1451
Fragments arranged by size:
1536 1451 936 658 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2516 3090 4353 4751 5025 5172 5583 2516
Size: 574 1263 398 274 147 411 2853
Fragments arranged by size:
2853 1263 574 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 3107 3137 1178
Size: 30 165 30 1356 30 318 30 3961
Fragments arranged by size:
3961 1356 318 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2669 2672 2825 2900 3197 1186
Size: 1000 483 3 153 75 297 3909
Fragments arranged by size:
3909 1000 483 297 153 75 3
BseYI C'CCAG_C
Cuts at: 2236 3153 5123 5619 2236
Size: 917 1970 496 2537
Fragments arranged by size:
2537 1970 917 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2128 1163
Size: 573 392 4955
Fragments arranged by size:
4955 573 392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3370 4131 5159 1540
Size: 275 186 1369 761 1028 2301
Fragments arranged by size:
2301 1369 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2709 3012 3113 3146 3981 4066 5137 2709
Size: 303 101 33 835 85 1071 3492
Fragments arranged by size:
3492 1071 835 303 101 85 33
BsmI GAATG_Cn'
Cuts at: 2526 3431 3524 2526
Size: 905 93 4922
Fragments arranged by size:
4922 905 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2236 2319 2709 3012 3113 3146
Size: 399 364 82 83 390 303 101 33
Cuts at: 3146 3173 3189 3239 3981 4066 5137 1391
Size: 27 16 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 364 303 101
85 83 82 50 33 27 16
BspEI T'CCGG_A
Cuts at: 5583 5583
Size: 5920
BspHI T'CATG_A
Cuts at: 3105 3809 5539 3105
Size: 704 1730 3486
Fragments arranged by size:
3486 1730 704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 2231 3275 844
Size: 1256 131 1044 3489
Fragments arranged by size:
3489 1256 1044 131
BsrBI CCG'CTC
Cuts at: 1394 1823 2662 2722 3807 1394
Size: 429 839 60 1085 3507
Fragments arranged by size:
3507 1085 839 429 60
BsrDI GCAATG_nn'
Cuts at: 65 1248 4415 4589 65
Size: 1183 3167 174 1396
Fragments arranged by size:
3167 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2862 4564 4751 1700
Size: 110 1052 1702 187 2869
Fragments arranged by size:
2869 1702 1052 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5920
BssSI C'ACGA_G
Cuts at: 2683 3980 4992 2683
Size: 1297 1012 3611
Fragments arranged by size:
3611 1297 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5920
BstBI TT'CG_AA
Cuts at: 4724 4724
Size: 5920
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5920
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2253 2038
Size: 215 5705
BstYI r'GATC_y
Cuts at: 1972 2205 2388 3250 3617 4002 4019 5471 5557
Size: 233 183 862 367 385 17 1452 86
Cuts at: 5557 5569 5915 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 862 385 367 346 233 183
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2433 2592 513
Size: 552 939 27 402 159 3841
Fragments arranged by size:
3841 939 552 402 159 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5181
Fragments arranged by size:
5181 683 56
BtsI GCAGTG_nn'
Cuts at: 3436 4230 4250 3436
Size: 794 20 5106
Fragments arranged by size:
5106 794 20
ClaI AT'CG_AT
Cuts at: 1554 2157 2601 1554
Size: 603 444 4873
Fragments arranged by size:
4873 603 444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5885
DraI TTT'AAA
Cuts at: 3224 3224
Size: 5920
DraIII CAC_nnn'GTG
Cuts at: 2106 2106
Size: 5920
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4927 809
Size: 507 3611 1802
Fragments arranged by size:
3611 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3367 4256 5885
Size: 54 1425 50 275 18 1537 889 1629
Cuts at: 5885 8
Size: 43
Fragments arranged by size:
1629 1537 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3367 1537
Size: 275 1555 4090
Fragments arranged by size:
4090 1555 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3850 1353
Size: 2497 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2870 4507 4881 5027 387
Size: 1064 138 1281 1637 374 146 1280
Fragments arranged by size:
1637 1281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3010 3144 3010
Size: 134 5786
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2499 2533 2828 2855 2861 2870
Size: 393 521 346 34 295 27 6 9
Cuts at: 2870 2903 2960 3150 3983 4580 5367 5635 1239
Size: 33 57 190 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 597 521 393 346 295
268 190 57 34 33 27 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3164 3164
Size: 5920
EcoO109I rG'GnC_Cy
Cuts at: 2845 3194 2845
Size: 349 5571
EcoRI G'AATT_C
Cuts at: 3229 3607 3229
Size: 378 5542
FspI TGC'GCA
Cuts at: 4426 4426
Size: 5920
HaeII r_GCGC'y
Cuts at: 1678 2541 2673 3072 3160 5067 1678
Size: 863 132 399 88 1907 2531
Fragments arranged by size:
2531 1907 863 399 132 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 3107 3139 4561
Size: 32 135 32 578 32 925 32 1422
Cuts at: 4561 4593 4635 4667 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 925 578 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3264 3510 669
Size: 988 281 1326 246 3079
Fragments arranged by size:
3079 1326 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5920
HpaI GTT'AAC
Cuts at: 3510 3510
Size: 5920
KasI G'GCGC_C
Cuts at: 3068 3068
Size: 5920
KpnI G_GTAC'C
Cuts at: 3245 3245
Size: 5920
MfeI C'AATT_G
Cuts at: 3519 3519
Size: 5920
MluI A'CGCG_T
Cuts at: 5796 5796
Size: 5920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5034 5218 1305
Size: 3729 184 2007
Fragments arranged by size:
3729 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4098
Fragments arranged by size:
4098 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2519 3878 4237 4396 518
Size: 648 1353 1359 359 159 2042
Fragments arranged by size:
2042 1359 1353 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2147 2275 2742 2839 2875 2881 2956 3121
Size: 541 128 467 97 36 6 75 165
Cuts at: 3121 3128 3336 4013 5161 5406 1606
Size: 7 208 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 541 467 245 208 165
128 97 75 36 7 6
NarI GG'CG_CC
Cuts at: 3069 3069
Size: 5920
NcoI C'CATG_G
Cuts at: 513 1065 2004 2031 513
Size: 552 939 27 4402
Fragments arranged by size:
4402 939 552 27
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5920
NotI GC'GGCC_GC
Cuts at: 3367 3367
Size: 5920
NspI r_CATG'y
Cuts at: 1132 1229 2419 3278 4823 1132
Size: 97 1190 859 1545 2229
Fragments arranged by size:
2229 1545 1190 859 97
PasI CC'CwG_GG
Cuts at: 1348 2995 3189 1348
Size: 1647 194 4079
Fragments arranged by size:
4079 1647 194
PciI A'CATG_T
Cuts at: 4819 4819
Size: 5920
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3160 1108
Size: 2052 3868
PfoI T'CCnGG_A
Cuts at: 2574 2937 2574
Size: 363 5557
PmeI GTTT'AAAC
Cuts at: 3224 3224
Size: 5920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 3989 4021 5502 5534 2759
Size: 32 1198 32 1481 32 3145
Fragments arranged by size:
3145 1481 1198 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2845 3194 2845
Size: 349 5571
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5920
PsiI TTA'TAA
Cuts at: 3490 3490
Size: 5920
PstI C_TGCA'G
Cuts at: 830 2309 2880 2949 3272 830
Size: 1479 571 69 323 3478
Fragments arranged by size:
3478 1479 571 323 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5920
PvuII CAG'CTG
Cuts at: 2742 2839 2875 2881 2956 3128 2742
Size: 97 36 6 75 172 5534
Fragments arranged by size:
5534 172 97 75 36 6
SacI G_AGCT'C
Cuts at: 3012 3146 3012
Size: 134 5786
SalI G'TCGA_C
Cuts at: 1936 3262 1936
Size: 1326 4594
SanDI GG'GwC_CC
Cuts at: 3194 3194
Size: 5920
SbfI CC_TGCA'GG
Cuts at: 3272 3272
Size: 5920
ScaI AGT'ACT
Cuts at: 4168 5880 4168
Size: 1712 4208
SfcI C'TryA_G
Cuts at: 826 1045 2305 2876 2945 3268 4403 5084 5275
Size: 219 1260 571 69 323 1135 681 191
Cuts at: 5275 826
Size: 1471
Fragments arranged by size:
1471 1260 1135 681 571 323 219 191
69
SfoI GGC'GCC
Cuts at: 3070 3070
Size: 5920
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5920
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5920
SmaI CCC'GGG
Cuts at: 1872 2369 3247 1872
Size: 497 878 4545
Fragments arranged by size:
4545 878 497
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4024 4925 5187 820
Size: 197 926 2081 901 262 1553
Fragments arranged by size:
2081 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3711 493
Size: 3218 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5920
SphI G_CATG'C
Cuts at: 1132 3278 1132
Size: 2146 3774
SrfI GCCC'GGGC
Cuts at: 2369 2369
Size: 5920
SspI AAT'ATT
Cuts at: 5 52 3844 5
Size: 47 3792 2081
Fragments arranged by size:
3792 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5920
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2373 3003 3359 513
Size: 552 939 27 342 630 356 3074
Fragments arranged by size:
3074 939 630 552 356 342 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3947 3964 4117 4302 4747 325
Size: 1255 2367 17 153 185 445 1498
Fragments arranged by size:
2367 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 4166 5878
Size: 274 80 33 51 1443 162 2027 1712
Cuts at: 5878 96
Size: 138
Fragments arranged by size:
2027 1712 1443 274 162 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5880 5912 5880
Size: 32 5888
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5920
XbaI T'CTAG_A
Cuts at: 3256 3256
Size: 5920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2035
Size: 5920
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5920
XmaI C'CCGG_G
Cuts at: 1870 2367 3245 1870
Size: 497 878 4545
Fragments arranged by size:
4545 878 497
XmnI GAAnn'nnTTC
Cuts at: 4049 4049
Size: 5920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3728 276
Size: 53 83 186 936 2194 2468
Fragments arranged by size:
2468 2194 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SapI SexAI SfiI SwaI