Restriction map for
pFN21AE3967
(Circular) (Six-base) MAPSORT of: pFN21AE3967.seq Check: 510 from: 1 to: 5917
pFN21AE3967 5917 bp
With 182 enzymes: SgfI *
March 28, 2012 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2601 2601
Size: 5917
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2877 3727 278
Size: 53 83 186 936 1341 850 2468
Fragments arranged by size:
2468 1341 936 850 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3260 1937
Size: 1323 4594
Acc65I G'GTAC_C
Cuts at: 3238 3238
Size: 5917
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 3131 3980 5364 5632 1632
Size: 652 847 849 1384 268 1917
Fragments arranged by size:
1917 1384 849 847 652 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 2691 4816 5793 1203
Size: 1488 2125 977 1327
Fragments arranged by size:
2125 1488 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2168
Size: 32 5885
AlwNI CAG_nnn'CTG
Cuts at: 2961 3660 5232 2961
Size: 699 1572 3646
Fragments arranged by size:
3646 1572 699
ApaI G_GGCC'C
Cuts at: 2968 2968
Size: 5917
ApaLI G'TGCA_C
Cuts at: 3974 5130 3974
Size: 1156 4761
ApoI r'AATT_y
Cuts at: 1493 3226 3452 3604 5713 1493
Size: 1733 226 152 2109 1697
Fragments arranged by size:
2109 1733 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4471 160
Size: 872 3439 1606
Fragments arranged by size:
3439 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 2048 3242 1870
Size: 73 105 1194 4545
Fragments arranged by size:
4545 1194 105 73
AvrII C'CTAG_G
Cuts at: 3092 3092
Size: 5917
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2775 2808 2775
Size: 33 5884
BamHI G'GATC_C
Cuts at: 3247 3614 3247
Size: 367 5550
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 3238 4693 618
Size: 325 217 627 265 1186 1455 1842
Fragments arranged by size:
1842 1455 1186 627 325 265 217
BanII G_rGCy'C
Cuts at: 1391 2436 2968 3236 1391
Size: 1045 532 268 4072
Fragments arranged by size:
4072 1045 532 268
BbeI G_GCGC'C
Cuts at: 2056 2056
Size: 5917
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2267 928
Size: 971 368 4578
Fragments arranged by size:
4578 971 368
BbvCI CC'TCA_GC
Cuts at: 2825 2825
Size: 5917
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2936 4528
Size: 107 122 71 631 605 337 927 1592
Cuts at: 4528 136
Size: 1525
Fragments arranged by size:
1592 1525 927 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2412 2476 2894 5912 2412
Size: 64 418 3018 2417
Fragments arranged by size:
3018 2417 418 64
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2341 2361 2968 3978 5134 1790
Size: 551 20 607 1010 1156 2573
Fragments arranged by size:
2573 1156 1010 607 551 20
BmgBI CAC'GTC
Cuts at: 1318 3073 1318
Size: 1755 4162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2487 3648 4606
Size: 416 53 83 399 24 1070 1161 958
Cuts at: 4606 442
Size: 1753
Fragments arranged by size:
1753 1161 1070 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2113 2673 2895 4577 1239
Size: 874 560 222 1682 2579
Fragments arranged by size:
2579 1682 874 560 222
Bpu10I CC'TnA_GC
Cuts at: 2825 2825
Size: 5917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2027 2121 4042 4907 5205 2027
Size: 94 1921 865 298 2739
Fragments arranged by size:
2739 1921 865 298 94
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2139 2518 4580 882
Size: 617 640 379 2062 2219
Fragments arranged by size:
2219 2062 640 617 379
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 2411 5911 2411
Size: 3500 2417
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2053 2874 3724 4106
Size: 53 83 186 936 520 821 850 382
Cuts at: 4106 4741 275
Size: 635 1451
Fragments arranged by size:
1451 936 850 821 635 520 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4350 4748 5022 5169 5580 4350
Size: 398 274 147 411 4687
Fragments arranged by size:
4687 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 1178
Size: 30 165 30 765 30 4897
Fragments arranged by size:
4897 765 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2942 1186
Size: 1006 750 4161
Fragments arranged by size:
4161 1006 750
BseYI C'CCAG_C
Cuts at: 2321 5120 5616 2321
Size: 2799 496 2622
Fragments arranged by size:
2799 2622 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 3004 1163
Size: 573 708 560 4076
Fragments arranged by size:
4076 708 573 560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2645 3978 4063 5134 2645
Size: 1333 85 1071 3428
Fragments arranged by size:
3428 1333 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
BsmBI CGTCTCn'nnnn_
Cuts at: 2225 2225
Size: 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 2361 2436 2645 2968 3236 3978
Size: 399 551 20 75 209 323 268 742
Cuts at: 3978 4063 5134 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 399 323 268 209
85 75 20
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2601 3272 844
Size: 1757 671 3489
Fragments arranged by size:
3489 1757 671
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2985 3045 4412 4586 65
Size: 1183 1737 60 1367 174 1396
Fragments arranged by size:
1737 1396 1367 1183 174 60
BsrFI r'CCGG_y
Cuts at: 1700 1810 2813 4561 4748 1700
Size: 110 1003 1748 187 2869
Fragments arranged by size:
2869 1748 1003 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5917
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 3977 4989 3977
Size: 1012 4905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2640 794
Size: 1846 4071
BstBI TT'CG_AA
Cuts at: 3136 4721 3136
Size: 1585 4332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2412 2476 2894 2958 3247 3614 3999 4016
Size: 440 64 418 64 289 367 385 17
Cuts at: 4016 5468 5554 5566 5912 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 440 418 385 367 346 289
86 64 64 17 12
Bsu36I CC'TnA_GG
Cuts at: 2954 2954
Size: 5917
BtgI C'CryG_G
Cuts at: 513 1065 2004 2306 2859 2931 513
Size: 552 939 302 553 72 3499
Fragments arranged by size:
3499 939 553 552 302 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 2143 2603 2731 3167 3433 4227 4247 2143
Size: 460 128 436 266 794 20 3813
Fragments arranged by size:
3813 794 460 436 266 128 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 3221 3221
Size: 5917
DraIII CAC_nnn'GTG
Cuts at: 2495 3038 2495
Size: 543 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2873 4924 809
Size: 507 1557 2051 1802
Fragments arranged by size:
2051 1802 1557 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2168 2455
Size: 54 1425 50 275 18 204 134 287
Cuts at: 2455 3067 3364 4253 5882 8
Size: 612 297 889 1629 43
Fragments arranged by size:
1629 1425 889 612 297 287 275 204
134 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2541 3847 1353
Size: 1188 1306 3423
Fragments arranged by size:
3423 1306 1188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2113 2284 2673 2895 3131 3980 4577
Size: 393 481 171 389 222 236 849 597
Cuts at: 4577 5364 5632 1239
Size: 787 268 1524
Fragments arranged by size:
1524 849 787 597 481 393 389 268
236 222 171
EcoO109I rG'GnC_Cy
Cuts at: 2548 2685 2803 2965 3097 2548
Size: 137 118 162 132 5368
Fragments arranged by size:
5368 162 137 132 118
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 5917
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 2056 5064 1678
Size: 378 3008 2531
Fragments arranged by size:
3008 2531 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2133 2165 4558
Size: 32 135 32 488 32 41 32 2393
Cuts at: 4558 4590 4632 4664 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2393 488 135 42 41 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2851 3261 3507 669
Size: 988 281 913 410 246 3079
Fragments arranged by size:
3079 988 913 410 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KasI G'GCGC_C
Cuts at: 2052 2052
Size: 5917
KpnI G_GTAC'C
Cuts at: 3242 3242
Size: 5917
MfeI C'AATT_G
Cuts at: 3516 3516
Size: 5917
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 5031 5215 1305
Size: 849 2877 184 2007
Fragments arranged by size:
2877 2007 849 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3069 10
Size: 54 1425 343 1237 2858
Fragments arranged by size:
2858 1425 1237 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2463 3875 4234 4393 518
Size: 648 1297 1412 359 159 2042
Fragments arranged by size:
2042 1412 1297 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2082 2651 3333 4010 5158 5403 1606
Size: 420 56 569 682 677 1148 245 2120
Fragments arranged by size:
2120 1148 682 677 569 420 245 56
NarI GG'CG_CC
Cuts at: 2053 2053
Size: 5917
NcoI C'CATG_G
Cuts at: 513 1065 2004 2859 513
Size: 552 939 855 3571
Fragments arranged by size:
3571 939 855 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 2638 3275 4820 1132
Size: 97 1409 637 1545 2229
Fragments arranged by size:
2229 1545 1409 637 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3038 1108
Size: 1930 3987
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2168 2200 3986 4018 5499 5531 2168
Size: 32 1786 32 1481 32 2554
Fragments arranged by size:
2554 1786 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2685 2803 3097 2548
Size: 137 118 294 5368
Fragments arranged by size:
5368 294 137 118
PshAI GACnn'nnGTC
Cuts at: 1613 2274 1613
Size: 661 5256
PsiI TTA'TAA
Cuts at: 3487 3487
Size: 5917
PspOMI G'GGCC_C
Cuts at: 2964 2964
Size: 5917
PstI C_TGCA'G
Cuts at: 830 2598 2633 2650 3269 830
Size: 1768 35 17 619 3478
Fragments arranged by size:
3478 1768 619 35 17
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2082 2651 2082
Size: 569 5348
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SanDI GG'GwC_CC
Cuts at: 2803 2803
Size: 5917
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 4165 5877 4165
Size: 1712 4205
SfcI C'TryA_G
Cuts at: 826 1045 2594 2629 2646 3265 4400 5081 5272
Size: 219 1549 35 17 619 1135 681 191
Cuts at: 5272 826
Size: 1471
Fragments arranged by size:
1549 1471 1135 681 619 219 191 35
17
SfoI GGC'GCC
Cuts at: 2054 2054
Size: 5917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 2813 1700
Size: 1113 4804
SmaI CCC'GGG
Cuts at: 1872 2050 3244 1872
Size: 178 1194 4545
Fragments arranged by size:
4545 1194 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2042 2136 4021 4922 5184 820
Size: 197 926 99 94 1885 901 262 1553
Fragments arranged by size:
1885 1553 926 901 262 197 99 94
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 2638 3275 1132
Size: 1506 637 3774
Fragments arranged by size:
3774 1506 637
SrfI GCCC'GGGC
Cuts at: 2050 2050
Size: 5917
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2004 2859 3092 3356 513
Size: 552 939 855 233 264 3074
Fragments arranged by size:
3074 939 855 552 264 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2335 2506 2653 2858 3944 3961 4114
Size: 1255 755 171 147 205 1086 17 153
Cuts at: 4114 4299 4744 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1086 755 445 205 185 171
153 147 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4163 5875 96
Size: 274 80 33 51 1443 2186 1712 138
Fragments arranged by size:
2186 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3013 3045 3159 3191 5877 5909 3013
Size: 32 114 32 2686 32 3021
Fragments arranged by size:
3021 2686 114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2118 2118
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 2048 3242 1870
Size: 178 1194 4545
Fragments arranged by size:
4545 1194 178
XmnI GAAnn'nnTTC
Cuts at: 3194 4046 3194
Size: 852 5065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2875 3725 276
Size: 53 83 186 936 1341 850 2468
Fragments arranged by size:
2468 1341 936 850 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI
EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SwaI