Restriction map for
pFN21AE3966
(Circular) (Six-base) MAPSORT of: pFN21AE3966.seq Check: 7886 from: 1 to: 5746
pFN21AE3966 5746 bp
With 182 enzymes: SgfI *
March 28, 2012 11:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2493 2729 3809 5193 5461 1632
Size: 861 236 1080 1384 268 1917
Fragments arranged by size:
1917 1384 1080 861 268 236
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 2385 4645 5622 1203
Size: 1182 2260 977 1327
Fragments arranged by size:
2260 1327 1182 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2618 1184
Size: 1434 4312
AlwNI CAG_nnn'CTG
Cuts at: 2106 2686 3489 5061 2106
Size: 580 803 1572 2791
Fragments arranged by size:
2791 1572 803 580
ApaLI G'TGCA_C
Cuts at: 2289 3803 4959 2289
Size: 1514 1156 3076
Fragments arranged by size:
3076 1514 1156
ApoI r'AATT_y
Cuts at: 1493 2967 3055 3281 3433 5542 1493
Size: 1474 88 226 152 2109 1697
Fragments arranged by size:
2109 1697 1474 226 152 88
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2409 3071 1870
Size: 73 466 662 4545
Fragments arranged by size:
4545 662 466 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2264 2697 2839 3065 1391
Size: 873 433 142 226 4072
Fragments arranged by size:
4072 873 433 226 142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 2634 928
Size: 971 623 112 4040
Fragments arranged by size:
4040 971 623 112
BbvCI CC'TCA_GC
Cuts at: 2450 2450
Size: 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 2281 1548
Size: 733 5013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2215 4357 136
Size: 107 122 71 631 605 543 2142 1525
Fragments arranged by size:
2142 1525 631 605 543 122 107 71
BglII A'GATC_T
Cuts at: 2704 5741 2704
Size: 3037 2709
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2293 3807 4963 1790
Size: 503 1514 1156 2573
Fragments arranged by size:
2573 1514 1156 503
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 2674 2905
Size: 416 53 83 399 24 1176 81 231
Cuts at: 2905 3477 4435 442
Size: 572 958 1753
Fragments arranged by size:
1753 1176 958 572 416 399 231 83
81 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 2462 2633 2729 4406 1239
Size: 1175 48 171 96 1677 2579
Fragments arranged by size:
2579 1677 1175 171 96 48
Bpu10I CC'TnA_GC
Cuts at: 2081 2243 2450 2081
Size: 162 207 5377
Fragments arranged by size:
5377 207 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2367 4409 882
Size: 617 868 2042 2219
Fragments arranged by size:
2219 2042 868 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2760 3553 3935 4570
Size: 53 83 186 936 1227 793 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1227 936 793 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 1178
Size: 30 165 30 837 30 4654
Fragments arranged by size:
4654 837 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2414 2603 2636 1186
Size: 865 363 189 33 4296
Fragments arranged by size:
4296 865 363 189 33
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2690 1163
Size: 573 954 4219
Fragments arranged by size:
4219 954 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2293 2373 2697 2839 3807 3892 4963 2293
Size: 80 324 142 968 85 1071 3076
Fragments arranged by size:
3076 1071 968 324 142 85 80
BsmI GAATG_Cn'
Cuts at: 2859 3257 3350 2859
Size: 398 93 5255
Fragments arranged by size:
5255 398 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2264 2293 2373 2697 2839 3065 3807
Size: 399 474 29 80 324 142 226 742
Cuts at: 3807 3892 4963 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 324 226 142
85 80 29
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 3101 844
Size: 1210 1047 3489
Fragments arranged by size:
3489 1210 1047
BsrBI CCG'CTC
Cuts at: 1394 1823 2359 3633 1394
Size: 429 536 1274 3507
Fragments arranged by size:
3507 1274 536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2602 4241 4415 65
Size: 1183 1354 1639 174 1396
Fragments arranged by size:
1639 1396 1354 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2719 794
Size: 1925 3821
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 2041 2378 1184
Size: 857 337 4552
Fragments arranged by size:
4552 857 337
BstXI CCAn_nnnn'nTGG
Cuts at: 2059 2233 2548 2059
Size: 174 315 5257
Fragments arranged by size:
5257 315 174
BstYI r'GATC_y
Cuts at: 1972 2704 3076 3443 3828 3845 5297 5383 5395
Size: 732 372 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 732 385 372 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2296 2374 513
Size: 552 939 292 78 3885
Fragments arranged by size:
3885 939 552 292 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2121 3262 4056 4076 2121
Size: 1141 794 20 3791
Fragments arranged by size:
3791 1141 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2336 4753 809
Size: 507 1020 2417 1802
Fragments arranged by size:
2417 1802 1020 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 3193 4082
Size: 54 1425 50 275 18 265 1098 889
Cuts at: 4082 5711 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1098 889 275 265 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2321 2469 2493 3676 1353
Size: 968 148 24 1183 3423
Fragments arranged by size:
3423 1183 968 148 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2695 2837 2695
Size: 142 5604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2414 2462 2493 2633 2729 3809 4406
Size: 393 782 48 31 140 96 1080 597
Cuts at: 4406 5193 5461 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1080 787 782 597 393 268 140
96 48 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2615 2396
Size: 219 5527
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2682 2714 4387 4419 4461
Size: 32 135 32 1110 32 1673 32 42
Cuts at: 4461 4493 1373
Size: 32 2626
Fragments arranged by size:
2626 1673 1110 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2505 748
Size: 1757 3989
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 2911 4860 5044 1305
Size: 1155 451 1949 184 2007
Fragments arranged by size:
2007 1949 1155 451 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3924
Fragments arranged by size:
3924 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3704 4063 4222 518
Size: 648 2538 359 159 2042
Fragments arranged by size:
2538 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3162 3839 4987 5232 1606
Size: 1556 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2296 513
Size: 552 939 292 3963
Fragments arranged by size:
3963 939 552 292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2118 2222 3104 4649 1132
Size: 97 889 104 882 1545 2229
Fragments arranged by size:
2229 1545 889 882 104 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5746
PfoI T'CCnGG_A
Cuts at: 2510 2577 2510
Size: 67 5679
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2882 1613
Size: 1269 4477
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 2724 3098 830
Size: 1894 374 3478
Fragments arranged by size:
3478 1894 374
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
SacI G_AGCT'C
Cuts at: 2697 2839 2697
Size: 142 5604
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2720 3094 4229 4910 5101 826
Size: 219 1675 374 1135 681 191 1471
Fragments arranged by size:
1675 1471 1135 681 374 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 2411 3073 1872
Size: 539 662 4545
Fragments arranged by size:
4545 662 539
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 2118 3104 1132
Size: 986 986 3774
Fragments arranged by size:
3774 986 986
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2296 3185 513
Size: 552 939 292 889 3074
Fragments arranged by size:
3074 939 889 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3790 3943 4128 4573 325
Size: 1255 2193 17 153 185 445 1498
Fragments arranged by size:
2193 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 5706 5738 2123
Size: 32 3551 32 2131
Fragments arranged by size:
3551 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2680 1316
Size: 1364 4382
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 2409 3071 1870
Size: 539 662 4545
Fragments arranged by size:
4545 662 539
XmnI GAAnn'nnTTC
Cuts at: 2631 2805 3875 2631
Size: 174 1070 4502
Fragments arranged by size:
4502 1070 174
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI