Restriction map for
pFN21AE3963
(Circular) (Six-base) MAPSORT of: pFN21AE3963.seq Check: 8238 from: 1 to: 6109
pFN21AE3963 6109 bp
With 182 enzymes: SgfI *
March 28, 2012 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2057 2057
Size: 6109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 4172 5556 5824 1632
Size: 1302 1238 1384 268 1917
Fragments arranged by size:
1917 1384 1302 1238 268
AfeI AGC'GCT
Cuts at: 2351 2975 3011 2351
Size: 624 36 5449
Fragments arranged by size:
5449 624 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 2157 5008 5985 1203
Size: 954 2851 977 1327
Fragments arranged by size:
2851 1327 977 954
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 2590 2593 2602 2956 3852 5424 2590
Size: 3 9 354 896 1572 3275
Fragments arranged by size:
3275 1572 896 354 9 3
ApaI G_GGCC'C
Cuts at: 2153 3070 2153
Size: 917 5192
ApaLI G'TGCA_C
Cuts at: 4166 5322 4166
Size: 1156 4953
ApoI r'AATT_y
Cuts at: 1493 3418 3644 3796 5905 1493
Size: 1925 226 152 2109 1697
Fragments arranged by size:
2109 1925 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 2165 2699 2830 3293 3307 3434
Size: 73 201 21 534 131 463 14 127
Cuts at: 3434 1870
Size: 4545
Fragments arranged by size:
4545 534 463 201 131 127 73 21
14
AvrII C'CTAG_G
Cuts at: 2656 2656
Size: 6109
BamHI G'GATC_C
Cuts at: 2077 3138 3439 3806 2077
Size: 1061 301 367 4380
Fragments arranged by size:
4380 1061 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2241 3430 4885 618
Size: 325 217 627 454 1189 1455 1842
Fragments arranged by size:
1842 1455 1189 627 454 325 217
BanII G_rGCy'C
Cuts at: 1391 2153 2688 2700 3070 3428 1391
Size: 762 535 12 370 358 4072
Fragments arranged by size:
4072 762 535 370 358 12
BbeI G_GCGC'C
Cuts at: 2245 2245
Size: 6109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2409 2549 2922 3139 928
Size: 971 510 140 373 217 3898
Fragments arranged by size:
3898 971 510 373 217 140
BbvCI CC'TCA_GC
Cuts at: 2810 2810
Size: 6109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2234 4720 136
Size: 107 122 71 631 605 562 2486 1525
Fragments arranged by size:
2486 1525 631 605 562 122 107 71
BglII A'GATC_T
Cuts at: 6104 6104
Size: 6109
BlpI GC'TnA_GC
Cuts at: 2682 3526 2682
Size: 844 5265
Bme1580I G_kGCm'C
Cuts at: 1790 2153 2165 2282 3070 4170 5326 1790
Size: 363 12 117 788 1100 1156 2573
Fragments arranged by size:
2573 1156 1100 788 363 117 12
BmgBI CAC'GTC
Cuts at: 1318 2869 1318
Size: 1551 4558
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 3043 3840
Size: 416 53 83 399 24 957 669 797
Cuts at: 3840 4798 442
Size: 958 1753
Fragments arranged by size:
1753 958 957 797 669 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2018 2441 2453 2624 2639 4769 1239
Size: 779 423 12 171 15 2130 2579
Fragments arranged by size:
2579 2130 779 423 171 15 12
Bpu10I CC'TnA_GC
Cuts at: 2810 2898 2810
Size: 88 6021
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 4234 5099 5397 2393
Size: 1841 865 298 3105
Fragments arranged by size:
3105 1841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 4772 882
Size: 617 984 2289 2219
Fragments arranged by size:
2289 2219 984 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 3362 6103 3362
Size: 2741 3368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2242 2751 3916 4298
Size: 53 83 186 936 709 509 1165 382
Cuts at: 4298 4933 275
Size: 635 1451
Fragments arranged by size:
1451 1165 936 709 635 509 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2080 2442 4542 4940 5214 5361 5772 2080
Size: 362 2100 398 274 147 411 2417
Fragments arranged by size:
2417 2100 411 398 362 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3385 3415 1178
Size: 30 165 30 1982 30 3872
Fragments arranged by size:
3872 1982 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2591 2594 2621 2624 2627 1186
Size: 868 537 3 27 3 3 4668
Fragments arranged by size:
4668 868 537 27 3 3 3
BseYI C'CCAG_C
Cuts at: 2133 2539 2998 3063 5312 5808 2133
Size: 406 459 65 2249 496 2434
Fragments arranged by size:
2434 2249 496 459 406 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2231 2842 2951 3100 1163
Size: 573 495 611 109 149 4172
Fragments arranged by size:
4172 611 573 495 149 109
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2384 2400 3374 3559 4320 5348
Size: 275 186 383 16 974 185 761 1028
Cuts at: 5348 1540
Size: 2301
Fragments arranged by size:
2301 1028 974 761 383 275 186 185
16
BsiHKAI G_wGCw'C
Cuts at: 2364 2688 2700 4170 4255 5326 2364
Size: 324 12 1470 85 1071 3147
Fragments arranged by size:
3147 1470 1071 324 85 12
BsiWI C'GTAC_G
Cuts at: 2154 2154
Size: 6109
BsmI GAATG_Cn'
Cuts at: 3620 3713 3620
Size: 93 6016
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2165 2282 2364 2688 2700 3070
Size: 399 363 12 117 82 324 12 370
Cuts at: 3070 3428 4170 4255 5326 1391
Size: 358 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 363 358 324
117 85 82 12 12
BspEI T'CCGG_A
Cuts at: 2442 5772 2442
Size: 3330 2779
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2057 2205 2501 3464 844
Size: 1213 148 296 963 3489
Fragments arranged by size:
3489 1213 963 296 148
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4604 4778 65
Size: 1183 3356 174 1396
Fragments arranged by size:
3356 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2260 3369 4753 4940 1700
Size: 110 450 1109 1384 187 2869
Fragments arranged by size:
2869 1384 1109 450 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6109
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 4169 5181 4169
Size: 1012 5097
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2377 2536 2590 2593 2956 794
Size: 1583 159 54 3 363 3947
Fragments arranged by size:
3947 1583 363 159 54 3
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 3214 3214
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2077 3138 3219 3297 3439 3806 4191 4208
Size: 105 1061 81 78 142 367 385 17
Cuts at: 4208 5660 5746 5758 6104 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1061 385 367 346 142 105
86 81 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 3019 3019
Size: 6109
BtgI C'CryG_G
Cuts at: 513 1065 2004 3120 513
Size: 552 939 1116 3502
Fragments arranged by size:
3502 1116 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2674 487
Size: 683 56 1448 3922
Fragments arranged by size:
3922 1448 683 56
BtsI GCAGTG_nn'
Cuts at: 2846 3275 3625 4419 4439 2846
Size: 429 350 794 20 4516
Fragments arranged by size:
4516 794 429 350 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DraIII CAC_nnn'GTG
Cuts at: 2377 2377
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2098 2226
Size: 54 1425 50 275 18 177 91 128
Cuts at: 2226 2301 2381 2397 3371 3556 4445 6074 8
Size: 75 80 16 974 185 889 1629 43
Fragments arranged by size:
1629 1425 974 889 275 185 177 128
91 80 75 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2381 2397 3371 3556 1537
Size: 275 569 16 974 185 4090
Fragments arranged by size:
4090 974 569 275 185 16
EarI CTCTTCn'nnn_
Cuts at: 1353 3332 4039 1353
Size: 1979 707 3423
Fragments arranged by size:
3423 1979 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2498 2938 4696 5070 5216 387
Size: 1064 138 909 440 1758 374 146 1280
Fragments arranged by size:
1758 1280 1064 909 440 374 146 138
EcoICRI GAG'CTC
Cuts at: 2686 2698 2686
Size: 12 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2018 2441 2453 2624 2639 2934 4172
Size: 393 386 423 12 171 15 295 1238
Cuts at: 4172 4769 5556 5824 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1238 787 597 423 393 386 295
268 171 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2948 2036
Size: 912 5197
EcoO109I rG'GnC_Cy
Cuts at: 2149 2827 2941 2953 3067 3199 2149
Size: 678 114 12 114 132 5059
Fragments arranged by size:
5059 678 132 114 114 12
EcoRI G'AATT_C
Cuts at: 3418 3796 3418
Size: 378 5731
FspI TGC'GCA
Cuts at: 2759 4615 2759
Size: 1856 4253
HaeII r_GCGC'y
Cuts at: 1678 2131 2245 2353 2673 2977 3013 5256 1678
Size: 453 114 108 320 304 36 2243 2531
Fragments arranged by size:
2531 2243 453 320 304 114 108 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4750 4782 4824 4856 1373
Size: 32 135 32 3178 32 42 32 2626
Fragments arranged by size:
3178 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6109
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KasI G'GCGC_C
Cuts at: 2241 2241
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 2157 5985 2157
Size: 3828 2281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5223 5407 1305
Size: 3918 184 2007
Fragments arranged by size:
3918 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4287
Fragments arranged by size:
4287 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4067 4426 4585 518
Size: 648 2901 359 159 2042
Fragments arranged by size:
2901 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2386 2527 2590 2709 2716 2935 3063 3122
Size: 780 141 63 119 7 219 128 59
Cuts at: 3122 3525 4202 5350 5595 1606
Size: 403 677 1148 245 2120
Fragments arranged by size:
2120 1148 780 677 403 245 219 141
128 119 63 59 7
NaeI GCC'GGC
Cuts at: 3371 3371
Size: 6109
NarI GG'CG_CC
Cuts at: 2242 2242
Size: 6109
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NgoMIV G'CCGG_C
Cuts at: 3369 3369
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 2381 3556 2381
Size: 1175 4934
NspI r_CATG'y
Cuts at: 1132 1229 3467 5012 1132
Size: 97 2238 1545 2229
Fragments arranged by size:
2238 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3070 1348
Size: 1722 4387
PciI A'CATG_T
Cuts at: 5008 5008
Size: 6109
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PfoI T'CCnGG_A
Cuts at: 2034 2766 3195 2034
Size: 732 429 4948
Fragments arranged by size:
4948 732 429
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 2941 3199 2827
Size: 114 258 5737
Fragments arranged by size:
5737 258 114
PshAI GACnn'nnGTC
Cuts at: 1613 2495 1613
Size: 882 5227
PsiI TTA'TAA
Cuts at: 3679 3679
Size: 6109
PspOMI G'GGCC_C
Cuts at: 2149 3066 2149
Size: 917 5192
PspXI vC'TCGA_Gb
Cuts at: 2699 2699
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2391 2598 2739 2784 3461 830
Size: 1561 207 141 45 677 3478
Fragments arranged by size:
3478 1561 677 207 141 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2590 2716 3063 2590
Size: 126 347 5636
Fragments arranged by size:
5636 347 126
SacI G_AGCT'C
Cuts at: 2688 2700 2688
Size: 12 6097
SacII CC_GC'GG
Cuts at: 3123 3123
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SanDI GG'GwC_CC
Cuts at: 2827 3199 2827
Size: 372 5737
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2387 2594 2735 2780 3256 3457 4592
Size: 219 1342 207 141 45 476 201 1135
Cuts at: 4592 5273 5464 826
Size: 681 191 1471
Fragments arranged by size:
1471 1342 1135 681 476 219 207 201
191 141 45
SfoI GGC'GCC
Cuts at: 2243 2243
Size: 6109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 2832 3436 1872
Size: 960 604 4545
Fragments arranged by size:
4545 960 604
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2699 4213 5114 5376 820
Size: 197 926 465 291 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 465 291 262 197
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3467 1132
Size: 2335 3774
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6109
StyI C'CwwG_G
Cuts at: 513 1065 2004 2656 2874 3183 3548 513
Size: 552 939 652 218 309 365 3074
Fragments arranged by size:
3074 939 652 552 365 309 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 2531 3220 4136 4153 4306 4491
Size: 1255 525 426 689 916 17 153 185
Cuts at: 4491 4936 325
Size: 445 1498
Fragments arranged by size:
1498 1255 916 689 525 445 426 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4355 6067 96
Size: 274 80 33 51 1443 2378 1712 138
Fragments arranged by size:
2378 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3385 3417 6069 6101 3385
Size: 32 2652 32 3393
Fragments arranged by size:
3393 2652 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6109
XbaI T'CTAG_A
Cuts at: 3445 3445
Size: 6109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3067
Size: 6109
XhoI C'TCGA_G
Cuts at: 1943 2699 1943
Size: 756 5353
XmaI C'CCGG_G
Cuts at: 1870 2830 3434 1870
Size: 960 604 4545
Fragments arranged by size:
4545 960 604
XmnI GAAnn'nnTTC
Cuts at: 4238 4238
Size: 6109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsmBI BstZ17I EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SapI SexAI SfiI SrfI SwaI