Restriction map for
pFN21AE3958
(Circular) (Six-base) MAPSORT of: pFN21AE3958.seq Check: 7150 from: 1 to: 6043
pFN21AE3958 6043 bp
With 182 enzymes: SgfI *
March 28, 2012 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2054 2054
Size: 6043
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2073 3386 1937
Size: 136 1313 4594
Fragments arranged by size:
4594 1313 136
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 3155 4106 5490 5758 1632
Size: 602 921 951 1384 268 1917
Fragments arranged by size:
1917 1384 951 921 602 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2106 2250 3786 5358 2106
Size: 144 1536 1572 2791
Fragments arranged by size:
2791 1572 1536 144
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 2218 2868 2953 3352 3578 3730 5839 1493
Size: 725 650 85 399 226 152 2109 1697
Fragments arranged by size:
2109 1697 725 650 399 226 152 85
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3368 1870
Size: 73 1425 4545
Fragments arranged by size:
4545 1425 73
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3364 4819 618
Size: 325 217 627 1577 1455 1842
Fragments arranged by size:
1842 1577 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2267 2374 2814 3055 3362 1391
Size: 876 107 440 241 307 4072
Fragments arranged by size:
4072 876 440 307 241 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2989 928
Size: 971 1090 3982
Fragments arranged by size:
3982 1090 971
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 6043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 2538 1548
Size: 990 5053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4654 136
Size: 107 122 71 631 605 2982 1525
Fragments arranged by size:
2982 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2963 6038 2963
Size: 3075 2968
BlpI GC'TnA_GC
Cuts at: 3460 3460
Size: 6043
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2533 4104 5260 1790
Size: 310 433 1571 1156 2573
Fragments arranged by size:
2573 1571 1156 433 310
BmgBI CAC'GTC
Cuts at: 1318 2942 1318
Size: 1624 4419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3028 3058 3167
Size: 416 53 83 399 24 1611 30 109
Cuts at: 3167 3774 4732 442
Size: 607 958 1753
Fragments arranged by size:
1753 1611 958 607 416 399 109 83
53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2527 2624 2723 4703 1239
Size: 1288 97 99 1980 2579
Fragments arranged by size:
2579 1980 1288 99 97
Bpu10I CC'TnA_GC
Cuts at: 2261 2403 2261
Size: 142 5901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4168 5033 5331 4168
Size: 865 298 4880
Fragments arranged by size:
4880 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4706 882
Size: 617 3207 2219
Fragments arranged by size:
3207 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3834 493
Size: 711 2630 2702
Fragments arranged by size:
2702 2630 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3850 4232 4867 275
Size: 53 83 186 936 2317 382 635 1451
Fragments arranged by size:
2317 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2566 4476 4874 5148 5295 5706 2566
Size: 1910 398 274 147 411 2903
Fragments arranged by size:
2903 1910 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2742 2772 2979 3009 1178
Size: 30 165 30 1339 30 207 30 4212
Fragments arranged by size:
4212 1339 207 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2498 1186
Size: 1138 174 4731
Fragments arranged by size:
4731 1138 174
BseYI C'CCAG_C
Cuts at: 2793 3289 5246 5742 2793
Size: 496 1957 496 3094
Fragments arranged by size:
3094 1957 496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2768 1163
Size: 573 1032 4438
Fragments arranged by size:
4438 1032 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3493 4254 5282 1540
Size: 275 186 1492 761 1028 2301
Fragments arranged by size:
2301 1492 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 2351 2367 2814 4104 4189 5260 2267
Size: 84 16 447 1290 85 1071 3050
Fragments arranged by size:
3050 1290 1071 447 85 84 16
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2267 2351 2367 2374 2533 2814
Size: 399 310 167 84 16 7 159 281
Cuts at: 2814 3055 3362 4104 4189 5260 1391
Size: 241 307 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 310 307 281 241
167 159 85 84 16 7
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2054 3398 844
Size: 1210 1344 3489
Fragments arranged by size:
3489 1344 1210
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2229 2521 4538 4712 65
Size: 1183 981 292 2017 174 1396
Fragments arranged by size:
2017 1396 1183 981 292 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2841 96
Size: 2745 3298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2963 3373 3740 4125 4142 5594 5680 5692
Size: 991 410 367 385 17 1452 86 12
Cuts at: 5692 6038 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 991 410 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 2814 3559 4353 4373 2814
Size: 745 794 20 4484
Fragments arranged by size:
4484 794 745 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3334 3490 4379
Size: 54 1425 50 275 18 1504 156 889
Cuts at: 4379 6008 8
Size: 1629 43
Fragments arranged by size:
1629 1504 1425 889 275 156 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 3973 1353
Size: 1035 1585 3423
Fragments arranged by size:
3423 1585 1035
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2265 2812 2265
Size: 547 5496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2527 2624 2723 3155 4106 4703
Size: 393 602 293 97 99 432 951 597
Cuts at: 4703 5490 5758 1239
Size: 787 268 1524
Fragments arranged by size:
1524 951 787 602 597 432 393 293
268 99 97
EcoNI CCTnn'n_nnAGG
Cuts at: 3079 3079
Size: 6043
EcoO109I rG'GnC_Cy
Cuts at: 2112 2286 2822 2112
Size: 174 536 5333
Fragments arranged by size:
5333 536 174
EcoRI G'AATT_C
Cuts at: 2953 3352 3730 2953
Size: 399 378 5266
Fragments arranged by size:
5266 399 378
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 5190 1678
Size: 3512 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2979 3011 4684 4716 4758
Size: 32 135 32 1407 32 1673 32 42
Cuts at: 4758 4790 1373
Size: 32 2626
Fragments arranged by size:
2626 1673 1407 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3103 3387 3633 669
Size: 988 281 1165 284 246 3079
Fragments arranged by size:
3079 1165 988 284 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3103 3633 3103
Size: 530 5513
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2909 5157 5341 1305
Size: 1071 533 2248 184 2007
Fragments arranged by size:
2248 2007 1071 533 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3336 10
Size: 54 1425 343 1504 2717
Fragments arranged by size:
2717 1504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4001 4360 4519 518
Size: 648 2835 359 159 2042
Fragments arranged by size:
2835 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 3293 3459 4136 5284 5529 1606
Size: 796 891 166 677 1148 245 2120
Fragments arranged by size:
2120 1148 891 796 677 245 166
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
NdeI CA'TA_TG
Cuts at: 387 3278 387
Size: 2891 3152
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 2027 2169 2791 3401 4946 1132
Size: 97 798 142 622 610 1545 2229
Fragments arranged by size:
2229 1545 798 622 610 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2250 2746 1108
Size: 1142 496 4405
Fragments arranged by size:
4405 1142 496
PfoI T'CCnGG_A
Cuts at: 2739 3074 2739
Size: 335 5708
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2286 2822 2286
Size: 536 5507
PshAI GACnn'nnGTC
Cuts at: 1613 2328 1613
Size: 715 5328
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 6011
PstI C_TGCA'G
Cuts at: 830 2821 3395 830
Size: 1991 574 3478
Fragments arranged by size:
3478 1991 574
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2402 3293 2402
Size: 891 5152
SacI G_AGCT'C
Cuts at: 2267 2814 2267
Size: 547 5496
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SanDI GG'GwC_CC
Cuts at: 2286 2286
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SexAI A'CCwGG_T
Cuts at: 2067 2067
Size: 6043
SfcI C'TryA_G
Cuts at: 826 1045 2107 2817 3391 4526 5207 5398 826
Size: 219 1062 710 574 1135 681 191 1471
Fragments arranged by size:
1471 1135 1062 710 681 574 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4147 5048 5310 820
Size: 197 926 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 2169 3401 1132
Size: 1037 1232 3774
Fragments arranged by size:
3774 1232 1037
SspI AAT'ATT
Cuts at: 5 52 2206 3967 5
Size: 47 2154 1761 2081
Fragments arranged by size:
2154 2081 1761 47
StuI AGG'CCT
Cuts at: 1517 3319 1517
Size: 1802 4241
StyI C'CwwG_G
Cuts at: 513 1065 2004 3482 513
Size: 552 939 1478 3074
Fragments arranged by size:
3074 1478 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4070 4087 4240 4425 4870 325
Size: 1255 2490 17 153 185 445 1498
Fragments arranged by size:
2490 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 4289 6001
Size: 274 80 33 51 1443 864 1448 1712
Cuts at: 6001 96
Size: 138
Fragments arranged by size:
1712 1448 1443 864 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 2742 2774 6003 6035 2013
Size: 32 697 32 3229 32 2021
Fragments arranged by size:
3229 2021 697 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2078 1316
Size: 762 5281
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2833 2833
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 2309 4172 2309
Size: 1863 4180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacII SapI
SfiI SfoI SrfI SwaI