Restriction map for
pFN21AE3956
(Circular) (Six-base) MAPSORT of: pFN21AE3956.seq Check: 4320 from: 1 to: 6238
pFN21AE3956 6238 bp
With 182 enzymes: SgfI *
March 28, 2012 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2470 2470
Size: 6238
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2635 2649 3581 1937
Size: 698 14 932 4594
Fragments arranged by size:
4594 932 698 14
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4301 5685 5953 1632
Size: 2669 1384 268 1917
Fragments arranged by size:
2669 1917 1384 268
AfeI AGC'GCT
Cuts at: 2307 2307
Size: 6238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 2216 3981 5553 2216
Size: 1765 1572 2901
Fragments arranged by size:
2901 1765 1572
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2700 3074 3199 3424 3547 3773 3925 6034
Size: 1207 374 125 225 123 226 152 2109
Cuts at: 6034 1493
Size: 1697
Fragments arranged by size:
2109 1697 1207 374 226 225 152 125
123
AseI AT'TA_AT
Cuts at: 160 1032 2601 4792 160
Size: 872 1569 2191 1606
Fragments arranged by size:
2191 1606 1569 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2277 3563 1870
Size: 73 334 1286 4545
Fragments arranged by size:
4545 1286 334 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 3559 5014 618
Size: 325 217 627 385 1387 1455 1842
Fragments arranged by size:
1842 1455 1387 627 385 325 217
BanII G_rGCy'C
Cuts at: 1391 3557 1391
Size: 2166 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3014 3088 3407 928
Size: 971 1115 74 319 3759
Fragments arranged by size:
3759 1115 971 319 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 3370 1548
Size: 1822 4416
BfrBI ATG'CAT
Cuts at: 2480 2480
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3220 6233 3220
Size: 3013 3225
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 4299 5455 1790
Size: 2509 1156 2573
Fragments arranged by size:
2573 2509 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3969 4927 442
Size: 416 53 83 399 24 2552 958 1753
Fragments arranged by size:
2552 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2780 2818 2780
Size: 38 6200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4898 1239
Size: 3659 2579
Bpu10I CC'TnA_GC
Cuts at: 2871 2871
Size: 6238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2750 4363 5228 5526 2750
Size: 1613 865 298 3462
Fragments arranged by size:
3462 1613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4901 882
Size: 617 3402 2219
Fragments arranged by size:
3402 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2112 4029 493
Size: 711 908 1917 2702
Fragments arranged by size:
2702 1917 908 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2632 4045 4427 5062
Size: 53 83 186 936 1099 1413 382 635
Cuts at: 5062 275
Size: 1451
Fragments arranged by size:
1451 1413 1099 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2572 2602 1178
Size: 30 165 30 1169 30 4814
Fragments arranged by size:
4814 1169 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6238
BseYI C'CCAG_C
Cuts at: 3033 5441 5937 3033
Size: 2408 496 3334
Fragments arranged by size:
3334 2408 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 1163
Size: 573 300 5365
Fragments arranged by size:
5365 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4299 4384 5455 4299
Size: 85 1071 5082
Fragments arranged by size:
5082 1071 85
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
BsmBI CGTCTCn'nnnn_
Cuts at: 3528 3528
Size: 6238
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3557 4299 4384 5455 1391
Size: 399 1767 742 85 1071 2174
Fragments arranged by size:
2174 1767 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 4127 5857 4127
Size: 1730 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 3593 844
Size: 1626 1123 3489
Fragments arranged by size:
3489 1626 1123
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4733 4907 65
Size: 1183 3485 174 1396
Fragments arranged by size:
3485 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4882 5069 1700
Size: 110 3072 187 2869
Fragments arranged by size:
3072 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6238
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6238
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 3220 3328 3568 3935 4320 4337 5789 5875
Size: 1248 108 240 367 385 17 1452 86
Cuts at: 5875 5887 6233 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1248 385 367 346 240 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2727 513
Size: 552 939 723 4024
Fragments arranged by size:
4024 939 723 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5499
Fragments arranged by size:
5499 683 56
BtsI GCAGTG_nn'
Cuts at: 3754 4548 4568 3754
Size: 794 20 5424
Fragments arranged by size:
5424 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 2181 2544 3127 3542 2181
Size: 363 583 415 4877
Fragments arranged by size:
4877 583 415 363
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3309 3685
Size: 54 1425 50 275 18 177 1302 376
Cuts at: 3685 4574 6203 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1302 889 376 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 3319 4168 1353
Size: 792 1174 849 3423
Fragments arranged by size:
3423 1174 849 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4301 4898 5685 5953 1239
Size: 393 2669 597 787 268 1524
Fragments arranged by size:
2669 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2521 2521
Size: 6238
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
EcoRV GAT'ATC
Cuts at: 2500 2500
Size: 6238
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2309 5385 1678
Size: 631 3076 2531
Fragments arranged by size:
3076 2531 631
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 2489 2521 3355
Size: 32 135 32 712 32 173 32 834
Cuts at: 3355 3387 4879 4911 4953 4985 1373
Size: 32 1492 32 42 32 2626
Fragments arranged by size:
2626 1492 834 712 173 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 3190 3582 3828 669
Size: 988 281 796 456 392 246 3079
Fragments arranged by size:
3079 988 796 456 392 281 246
HindIII A'AGCT_T
Cuts at: 748 2863 3047 3302 748
Size: 2115 184 255 3684
Fragments arranged by size:
3684 2115 255 184
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 2625 2811 3837 2625
Size: 186 1026 5026
Fragments arranged by size:
5026 1026 186
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2363 3411 5352 5536 1305
Size: 1058 1048 1941 184 2007
Fragments arranged by size:
2007 1941 1058 1048 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3311 10
Size: 54 1425 343 177 1302 2937
Fragments arranged by size:
2937 1425 1302 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4196 4555 4714 518
Size: 648 3030 359 159 2042
Fragments arranged by size:
3030 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3654 4331 5479 5724 1606
Size: 2048 677 1148 245 2120
Fragments arranged by size:
2120 2048 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2727 513
Size: 552 939 723 4024
Fragments arranged by size:
4024 939 723 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NsiI A_TGCA'T
Cuts at: 2482 2482
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 3596 5141 1132
Size: 97 2367 1545 2229
Fragments arranged by size:
2367 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2845 1108
Size: 1737 4501
PfoI T'CCnGG_A
Cuts at: 3271 3271
Size: 6238
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2521
Size: 6238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PspXI vC'TCGA_Gb
Cuts at: 2277 2277
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3590 830
Size: 2760 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 4486 6198 4486
Size: 1712 4526
SfcI C'TryA_G
Cuts at: 826 1045 3586 4721 5402 5593 826
Size: 219 2541 1135 681 191 1471
Fragments arranged by size:
2541 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2277 2765 4342 5243 5505 820
Size: 197 926 334 488 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 488 334 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 2071 1517
Size: 554 5684
StyI C'CwwG_G
Cuts at: 513 1065 2004 2727 3677 513
Size: 552 939 723 950 3074
Fragments arranged by size:
3074 950 939 723 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2898 3522 4265 4282 4435 4620 5065
Size: 1255 1318 624 743 17 153 185 445
Cuts at: 5065 325
Size: 1498
Fragments arranged by size:
1498 1318 1255 743 624 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4484 6196 96
Size: 274 80 33 51 1443 2507 1712 138
Fragments arranged by size:
2507 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 6198 6230 2780
Size: 32 3386 32 2788
Fragments arranged by size:
3386 2788 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 2277 1943
Size: 334 5904
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 2334 2829 4367 2334
Size: 495 1538 4205
Fragments arranged by size:
4205 1538 495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI