Restriction map for
pFN21AE3955
(Circular) (Six-base) MAPSORT of: pFN21AE3955.seq Check: 868 from: 1 to: 6970
pFN21AE3955 6970 bp
With 182 enzymes: SgfI *
March 28, 2012 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2893 2893
Size: 6970
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4780 278
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4313 1937
Size: 2376 4594
Acc65I G'GTAC_C
Cuts at: 3158 4206 4291 3158
Size: 1048 85 5837
Fragments arranged by size:
5837 1048 85
AclI AA'CG_TT
Cuts at: 1563 5097 5470 1563
Size: 3534 373 3063
Fragments arranged by size:
3534 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 5033 6417 6685 1632
Size: 1011 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 1011 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6773
AflIII A'CryG_T
Cuts at: 1203 5869 6846 1203
Size: 4666 977 1327
Fragments arranged by size:
4666 1327 977
AgeI A'CCGG_T
Cuts at: 5801 5801
Size: 6970
AhdI GACnn_n'nnGTC
Cuts at: 3520 5699 3520
Size: 2179 4791
AleI CACnn'nnGTG
Cuts at: 1166 3363 4193 1166
Size: 2197 830 3943
Fragments arranged by size:
3943 2197 830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6970
AlwNI CAG_nnn'CTG
Cuts at: 2195 2566 4713 6285 2195
Size: 371 2147 1572 2880
Fragments arranged by size:
2880 2147 1572 371
ApaI G_GGCC'C
Cuts at: 2800 3325 3757 2800
Size: 525 432 6013
Fragments arranged by size:
6013 525 432
ApaLI G'TGCA_C
Cuts at: 5027 6183 5027
Size: 1156 5814
ApoI r'AATT_y
Cuts at: 1493 4279 4505 4657 6766 1493
Size: 2786 226 152 2109 1697
Fragments arranged by size:
2786 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5524 160
Size: 872 4492 1606
Fragments arranged by size:
4492 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2204 2268 2487 2522 2751 2868
Size: 73 231 30 64 219 35 229 117
Cuts at: 2868 3285 3417 3558 4179 4295 1870
Size: 417 132 141 621 116 4545
Fragments arranged by size:
4545 621 417 231 229 219 141 132
117 116 73 64 35 30
BamHI G'GATC_C
Cuts at: 4300 4667 4300
Size: 367 6603
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2330 2361 2406 3025
Size: 325 217 627 305 238 31 45 619
Cuts at: 3025 3158 3629 4206 4291 5746 618
Size: 133 471 577 85 1455 1842
Fragments arranged by size:
1842 1455 627 619 577 471 325 305
238 217 133 85 45 31
BanII G_rGCy'C
Cuts at: 1391 2800 2842 3066 3078 3253 3301 3325 3426
Size: 1409 42 224 12 175 48 24 101
Cuts at: 3426 3543 3757 4052 4289 1391
Size: 117 214 295 237 4072
Fragments arranged by size:
4072 1409 295 237 224 214 175 117
101 48 42 24 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3212 928
Size: 971 1313 4686
Fragments arranged by size:
4686 1313 971
BbvCI CC'TCA_GC
Cuts at: 2781 2781
Size: 6970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2846 2880 5161 5195 1903
Size: 34 909 34 2281 34 3678
Fragments arranged by size:
3678 2281 909 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4862 6072 4862
Size: 1210 5760
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6970
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2804 2915 4035
Size: 107 122 71 631 605 1132 111 1120
Cuts at: 4035 4047 5581 136
Size: 12 1534 1525
Fragments arranged by size:
1534 1525 1132 1120 631 605 122 111
107 71 12
BglII A'GATC_T
Cuts at: 6965 6965
Size: 6970
BlpI GC'TnA_GC
Cuts at: 4387 4387
Size: 6970
Bme1580I G_kGCm'C
Cuts at: 1790 2095 2311 2409 2638 2800 3028 3325 3595
Size: 305 216 98 229 162 228 297 270
Cuts at: 3595 3632 3757 5031 6187 1790
Size: 37 125 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 305 297 270 229 228
216 162 125 98 37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6970
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 4701 5659
Size: 416 53 83 399 24 1083 2201 958
Cuts at: 5659 442
Size: 1753
Fragments arranged by size:
2201 1753 1083 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2729 3705 1056
Size: 1673 976 4321
Fragments arranged by size:
4321 1673 976
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 3670 2986
Size: 684 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2099 2672 2714 3080 3531 3903 5630
Size: 818 42 573 42 366 451 372 1727
Cuts at: 5630 1239
Size: 2579
Fragments arranged by size:
2579 1727 818 573 451 372 366 42
42
Bpu10I CC'TnA_GC
Cuts at: 2045 2781 2999 3758 2045
Size: 736 218 759 5257
Fragments arranged by size:
5257 759 736 218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3389 5095 5960 6258 2909
Size: 480 1706 865 298 3621
Fragments arranged by size:
3621 1706 865 480 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2274 5633 882
Size: 617 775 3359 2219
Fragments arranged by size:
3359 2219 775 617
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3835 4761 493
Size: 711 1641 990 926 2702
Fragments arranged by size:
2702 1641 990 926 711
BsaBI GATnn'nnATC
Cuts at: 6964 6964
Size: 6970
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2787 4777 5159 5794
Size: 53 83 186 936 1254 1990 382 635
Cuts at: 5794 275
Size: 1451
Fragments arranged by size:
1990 1451 1254 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4261 5403 5801 6075 6222 6633 4261
Size: 1142 398 274 147 411 4598
Fragments arranged by size:
4598 1142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6745
Fragments arranged by size:
6745 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 2786 3131 3188 3326 3455 1186
Size: 1309 291 345 57 138 129 4701
Fragments arranged by size:
4701 1309 345 291 138 129 57
BseYI C'CCAG_C
Cuts at: 2514 2824 3351 3425 3430 3994 6173 6669 2514
Size: 310 527 74 5 564 2179 496 2815
Fragments arranged by size:
2815 2179 564 527 496 310 74 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2398 2590 3761 1163
Size: 573 662 192 1171 4372
Fragments arranged by size:
4372 1171 662 573 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2161 4420 5181 6209 1540
Size: 275 186 160 2259 761 1028 2301
Fragments arranged by size:
2301 2259 1028 761 275 186 160
BsiHKAI G_wGCw'C
Cuts at: 3066 5031 5116 6187 3066
Size: 1965 85 1071 3849
Fragments arranged by size:
3849 1965 1071 85
BsmI GAATG_Cn'
Cuts at: 4481 4574 4481
Size: 93 6877
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2311 2409 2638 2800 2842 3028
Size: 399 305 216 98 229 162 42 186
Cuts at: 3028 3066 3078 3253 3301 3325 3426 3543 3595
Size: 38 12 175 48 24 101 117 52
Cuts at: 3595 3632 3757 4052 4289 5031 5116 6187 1391
Size: 37 125 295 237 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 305 295 237 229
216 186 175 162 125 117 101 98
85 52 48 42 38 37 24 12
BspEI T'CCGG_A
Cuts at: 4261 6633 4261
Size: 2372 4598
BspHI T'CATG_A
Cuts at: 4859 6589 4859
Size: 1730 5240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2292 2893 4194 4325 844
Size: 1448 601 1301 131 3489
Fragments arranged by size:
3489 1448 1301 601 131
BsrBI CCG'CTC
Cuts at: 1394 1823 4857 1394
Size: 429 3034 3507
Fragments arranged by size:
3507 3034 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5465 5639 65
Size: 1183 4217 174 1396
Fragments arranged by size:
4217 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2347 2666 3637 5614 5801 1700
Size: 110 537 319 971 1977 187 2869
Fragments arranged by size:
2869 1977 971 537 319 187 110
BsrGI T'GTAC_A
Cuts at: 96 3843 96
Size: 3747 3223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6970
BssSI C'ACGA_G
Cuts at: 3335 5030 6042 3335
Size: 1695 1012 4263
Fragments arranged by size:
4263 1695 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2577 2821 794
Size: 1783 244 4943
Fragments arranged by size:
4943 1783 244
BstBI TT'CG_AA
Cuts at: 5774 5774
Size: 6970
BstEII G'GTnAC_C
Cuts at: 1184 4183 1184
Size: 2999 3971
BstXI CCAn_nnnn'nTGG
Cuts at: 3137 3341 3365 4007 4195 3137
Size: 204 24 642 188 5912
Fragments arranged by size:
5912 642 204 188 24
BstYI r'GATC_y
Cuts at: 1972 2964 3564 4300 4667 5052 5069 6521 6607
Size: 992 600 736 367 385 17 1452 86
Cuts at: 6607 6619 6965 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 992 736 600 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3645 3645
Size: 6970
BtgI C'CryG_G
Cuts at: 513 1065 2004 2014 2352 4162 4191 513
Size: 552 939 10 338 1810 29 3292
Fragments arranged by size:
3292 1810 939 552 338 29 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6231
Fragments arranged by size:
6231 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 2531 3749 3791 4486 5280 5300 2102
Size: 429 1218 42 695 794 20 3772
Fragments arranged by size:
3772 1218 794 695 429 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6935
DraI TTT'AAA
Cuts at: 4274 4274
Size: 6970
DraIII CAC_nnn'GTG
Cuts at: 2440 3784 2440
Size: 1344 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5977 809
Size: 507 4661 1802
Fragments arranged by size:
4661 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2158 2349 2664
Size: 54 1425 50 275 18 328 191 315
Cuts at: 2664 2907 3669 3864 4417 5306 6935 8
Size: 243 762 195 553 889 1629 43
Fragments arranged by size:
1629 1425 889 762 553 328 315 275
243 195 191 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2158 4417 1537
Size: 275 346 2259 4090
Fragments arranged by size:
4090 2259 346 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3285 4900 1353
Size: 1932 1615 3423
Fragments arranged by size:
3423 1932 1615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 2897 3590 5557 5931 6077
Size: 1064 138 576 732 693 1967 374 146
Cuts at: 6077 387
Size: 1280
Fragments arranged by size:
1967 1280 1064 732 693 576 374 146
138
EcoICRI GAG'CTC
Cuts at: 3064 3064
Size: 6970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2099 2643 2672 2714 3080 3531
Size: 393 425 42 544 29 42 366 451
Cuts at: 3531 3903 5033 5630 6417 6685 1239
Size: 372 1130 597 787 268 1524
Fragments arranged by size:
1524 1130 787 597 544 451 425 393
372 366 268 42 42 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2036 2261 3767 2036
Size: 225 1506 5239
Fragments arranged by size:
5239 1506 225
EcoO109I rG'GnC_Cy
Cuts at: 2019 2041 2797 3321 3347 3753 3754 3901 4131
Size: 22 756 524 26 406 1 147 230
Cuts at: 4131 2019
Size: 4858
Fragments arranged by size:
4858 756 524 406 230 147 26 22
1
EcoRI G'AATT_C
Cuts at: 4279 4657 4279
Size: 378 6592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 6970
FseI GG_CCGG'CC
Cuts at: 2351 2351
Size: 6970
FspI TGC'GCA
Cuts at: 5476 5476
Size: 6970
HaeII r_GCGC'y
Cuts at: 1678 2497 2726 3914 6117 1678
Size: 819 229 1188 2203 2531
Fragments arranged by size:
2531 2203 1188 819 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3506 3538 5611 5643 5685
Size: 32 135 32 1934 32 2073 32 42
Cuts at: 5685 5717 1373
Size: 32 2626
Fragments arranged by size:
2626 2073 1934 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4102 4314 4560 669
Size: 988 281 2164 212 246 3079
Fragments arranged by size:
3079 2164 988 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6970
HpaI GTT'AAC
Cuts at: 4560 4560
Size: 6970
KpnI G_GTAC'C
Cuts at: 3162 4210 4295 3162
Size: 1048 85 5837
Fragments arranged by size:
5837 1048 85
MfeI C'AATT_G
Cuts at: 4569 4569
Size: 6970
MluI A'CGCG_T
Cuts at: 6846 6846
Size: 6970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3936 6084 6268 1305
Size: 2631 2148 184 2007
Fragments arranged by size:
2631 2148 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2909 3866 10
Size: 54 1425 343 1077 957 3114
Fragments arranged by size:
3114 1425 1077 957 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3339 3363 3503 4193 4928 5287 5446
Size: 648 2173 24 140 690 735 359 159
Cuts at: 5446 518
Size: 2042
Fragments arranged by size:
2173 2042 735 690 648 359 159 140
24
MspA1I CmG'CkG
Cuts at: 1606 2086 2146 2169 2412 2995 3007 3058 4386
Size: 480 60 23 243 583 12 51 1328
Cuts at: 4386 5063 6211 6456 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 583 480 245 243
60 51 23 12
NaeI GCC'GGC
Cuts at: 2349 2349
Size: 6970
NcoI C'CATG_G
Cuts at: 513 1065 2004 4191 513
Size: 552 939 2187 3292
Fragments arranged by size:
3292 2187 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6970
NgoMIV G'CCGG_C
Cuts at: 2347 2347
Size: 6970
NheI G'CTAG_C
Cuts at: 1052 2725 3701 1052
Size: 1673 976 4321
Fragments arranged by size:
4321 1673 976
NotI GC'GGCC_GC
Cuts at: 2158 4417 2158
Size: 2259 4711
NspI r_CATG'y
Cuts at: 1132 1229 4328 5873 1132
Size: 97 3099 1545 2229
Fragments arranged by size:
3099 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3021 3163 3436 3937 1348
Size: 1673 142 273 501 4381
Fragments arranged by size:
4381 1673 501 273 142
PciI A'CATG_T
Cuts at: 5869 5869
Size: 6970
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2440 3307 3784 4168 1108
Size: 1332 867 477 384 3910
Fragments arranged by size:
3910 1332 867 477 384
PfoI T'CCnGG_A
Cuts at: 2034 3078 3880 4116 2034
Size: 1044 802 236 4888
Fragments arranged by size:
4888 1044 802 236
PmeI GTTT'AAAC
Cuts at: 4274 4274
Size: 6970
PmlI CAC'GTG
Cuts at: 2845 3835 2845
Size: 990 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5039 5071 6552 6584 5039
Size: 32 1481 32 5425
Fragments arranged by size:
5425 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2019 3347 4131 2019
Size: 1328 784 4858
Fragments arranged by size:
4858 1328 784
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6970
PsiI TTA'TAA
Cuts at: 4540 4540
Size: 6970
PspOMI G'GGCC_C
Cuts at: 2796 3321 3753 2796
Size: 525 432 6013
Fragments arranged by size:
6013 525 432
PstI C_TGCA'G
Cuts at: 830 3012 3491 3681 4322 830
Size: 2182 479 190 641 3478
Fragments arranged by size:
3478 2182 641 479 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6970
PvuII CAG'CTG
Cuts at: 2086 2146 3007 3058 2086
Size: 60 861 51 5998
Fragments arranged by size:
5998 861 60 51
SacI G_AGCT'C
Cuts at: 3066 3066
Size: 6970
SalI G'TCGA_C
Cuts at: 1936 4312 1936
Size: 2376 4594
SanDI GG'GwC_CC
Cuts at: 4131 4131
Size: 6970
SbfI CC_TGCA'GG
Cuts at: 4322 4322
Size: 6970
ScaI AGT'ACT
Cuts at: 5218 6930 5218
Size: 1712 5258
SexAI A'CCwGG_T
Cuts at: 2256 2448 2682 4209 2256
Size: 192 234 1527 5017
Fragments arranged by size:
5017 1527 234 192
SfcI C'TryA_G
Cuts at: 826 1045 2458 3008 3487 3677 4318 5453 6134
Size: 219 1413 550 479 190 641 1135 681
Cuts at: 6134 6325 826
Size: 191 1471
Fragments arranged by size:
1471 1413 1135 681 641 550 479 219
191 190
SfiI GGCCn_nnn'nGGCC
Cuts at: 2804 2804
Size: 6970
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6970
SmaI CCC'GGG
Cuts at: 1872 2270 2489 2524 2753 3419 3560 4181 4297
Size: 398 219 35 229 666 141 621 116
Cuts at: 4297 1872
Size: 4545
Fragments arranged by size:
4545 666 621 398 229 219 141 116
35
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 3404 5074 5975 6237 820
Size: 197 926 945 516 1670 901 262 1553
Fragments arranged by size:
1670 1553 945 926 901 516 262 197
SnaBI TAC'GTA
Cuts at: 493 4761 493
Size: 4268 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6970
SphI G_CATG'C
Cuts at: 1132 4328 1132
Size: 3196 3774
SrfI GCCC'GGGC
Cuts at: 2270 2270
Size: 6970
SspI AAT'ATT
Cuts at: 5 52 4894 5
Size: 47 4842 2081
Fragments arranged by size:
4842 2081 47
StuI AGG'CCT
Cuts at: 1517 2393 4029 1517
Size: 876 1636 4458
Fragments arranged by size:
4458 1636 876
StyI C'CwwG_G
Cuts at: 513 1065 2004 3306 4138 4191 4409 513
Size: 552 939 1302 832 53 218 3074
Fragments arranged by size:
3074 1302 939 832 552 218 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2110 2315 2447 3542 4997 5014 5167
Size: 1255 530 205 132 1095 1455 17 153
Cuts at: 5167 5352 5797 325
Size: 185 445 1498
Fragments arranged by size:
1498 1455 1255 1095 530 445 205 185
153 132 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3843 4014 5216
Size: 274 80 33 51 1443 1866 171 1202
Cuts at: 5216 6928 96
Size: 1712 138
Fragments arranged by size:
1866 1712 1443 1202 274 171 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2523 2555 6930 6962 2523
Size: 32 4375 32 2531
Fragments arranged by size:
4375 2531 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6970
XbaI T'CTAG_A
Cuts at: 4306 4306
Size: 6970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3137 3242 3433 3781 3137
Size: 105 191 348 6326
Fragments arranged by size:
6326 348 191 105
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6970
XmaI C'CCGG_G
Cuts at: 1870 2268 2487 2522 2751 3417 3558 4179 4295
Size: 398 219 35 229 666 141 621 116
Cuts at: 4295 1870
Size: 4545
Fragments arranged by size:
4545 666 621 398 229 219 141 116
35
XmnI GAAnn'nnTTC
Cuts at: 3389 3602 5099 3389
Size: 213 1497 5260
Fragments arranged by size:
5260 1497 213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4778 276
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoRV FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SfoI SwaI