Restriction map for
pFN21AE3950
(Circular) (Six-base) MAPSORT of: pFN21AE3950.seq Check: 6933 from: 1 to: 7054
pFN21AE3950 7054 bp
With 182 enzymes: SgfI *
March 28, 2012 11:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2145 3905 2145
Size: 1760 5294
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2603 2688 4864 278
Size: 53 83 186 936 1067 85 2176 2468
Fragments arranged by size:
2468 2176 1067 936 186 85 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 1937
Size: 2460 4594
Acc65I G'GTAC_C
Cuts at: 2568 4375 2568
Size: 1807 5247
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 4219 5117 6501 6769 1632
Size: 407 2180 898 1384 268 1917
Fragments arranged by size:
2180 1917 1384 898 407 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6857
AflIII A'CryG_T
Cuts at: 1203 2909 5953 6930 1203
Size: 1706 3044 977 1327
Fragments arranged by size:
3044 1706 1327 977
AgeI A'CCGG_T
Cuts at: 3978 5885 3978
Size: 1907 5147
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 3236 1166
Size: 2070 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2232 2561 1184
Size: 1048 329 5677
Fragments arranged by size:
5677 1048 329
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3347 3379 3347
Size: 32 7022
AlwNI CAG_nnn'CTG
Cuts at: 2137 4797 6369 2137
Size: 2660 1572 2822
Fragments arranged by size:
2822 2660 1572
ApaI G_GGCC'C
Cuts at: 2805 4226 2805
Size: 1421 5633
ApaLI G'TGCA_C
Cuts at: 3953 5111 6267 3953
Size: 1158 1156 4740
Fragments arranged by size:
4740 1158 1156
ApoI r'AATT_y
Cuts at: 1493 4363 4589 4741 6850 1493
Size: 2870 226 152 2109 1697
Fragments arranged by size:
2870 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 2353 2430 2549 2727 2854 2915
Size: 73 217 193 77 119 178 127 61
Cuts at: 2915 3753 4379 1870
Size: 838 626 4545
Fragments arranged by size:
4545 838 626 217 193 178 127 119
77 73 61
BamHI G'GATC_C
Cuts at: 4384 4751 4384
Size: 367 6687
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2395 2435 2568 2810
Size: 325 217 627 396 212 40 133 242
Cuts at: 2810 2822 3434 3538 3665 4375 5830 618
Size: 12 612 104 127 710 1455 1842
Fragments arranged by size:
1842 1455 710 627 612 396 325 242
217 212 133 127 104 40 12
BanII G_rGCy'C
Cuts at: 1391 2471 2805 2855 4226 4373 1391
Size: 1080 334 50 1371 147 4072
Fragments arranged by size:
4072 1371 1080 334 147 50
BbeI G_GCGC'C
Cuts at: 2187 2826 3438 2187
Size: 639 612 5803
Fragments arranged by size:
5803 639 612
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2454 3719 4246 928
Size: 971 555 1265 527 3736
Fragments arranged by size:
3736 1265 971 555 527
BbvCI CC'TCA_GC
Cuts at: 4268 4268
Size: 7054
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5245 5279 1903
Size: 34 3308 34 3678
Fragments arranged by size:
3678 3308 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 3345 3393 1548
Size: 1797 48 5209
Fragments arranged by size:
5209 1797 48
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 2526 2859
Size: 107 122 71 631 605 599 255 333
Cuts at: 2859 5665 136
Size: 2806 1525
Fragments arranged by size:
2806 1525 631 605 599 333 255 122
107 71
BglII A'GATC_T
Cuts at: 3378 7049 3378
Size: 3671 3383
BlpI GC'TnA_GC
Cuts at: 2309 4471 2309
Size: 2162 4892
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2483 2805 3932 3957 4226 5115 6271
Size: 610 83 322 1127 25 269 889 1156
Cuts at: 6271 1790
Size: 2573
Fragments arranged by size:
2573 1156 1127 889 610 322 269 83
25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2889 3970 4785
Size: 416 53 83 399 24 1472 1081 815
Cuts at: 4785 5743 442
Size: 958 1753
Fragments arranged by size:
1753 1472 1081 958 815 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 7054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 3005 3359 3461 3584 3625 3671 5714
Size: 986 780 354 102 123 41 46 2043
Cuts at: 5714 1239
Size: 2579
Fragments arranged by size:
2579 2043 986 780 354 123 102 46
41
Bpu10I CC'TnA_GC
Cuts at: 4268 4268
Size: 7054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2696 5179 6044 6342 2345
Size: 351 2483 865 298 3057
Fragments arranged by size:
3057 2483 865 351 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2286 5717 882
Size: 617 787 3431 2219
Fragments arranged by size:
3431 2219 787 617
BsaAI yAC'GTr
Cuts at: 493 1204 2478 2910 3973 4243 4845 493
Size: 711 1274 432 1063 270 602 2702
Fragments arranged by size:
2702 1274 1063 711 602 432 270
BsaBI GATnn'nnATC
Cuts at: 7048 7048
Size: 7054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2184 2600 2685 2823
Size: 53 83 186 936 651 416 85 138
Cuts at: 2823 3435 4861 5243 5878 275
Size: 612 1426 382 635 1451
Fragments arranged by size:
1451 1426 936 651 635 612 416 382
186 138 85 83 53
BsaWI w'CCGG_w
Cuts at: 3317 3978 5487 5885 6159 6306 6717 3317
Size: 661 1509 398 274 147 411 3654
Fragments arranged by size:
3654 1509 661 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3347 3377 3695 3725 4097
Size: 30 165 30 1944 30 318 30 372
Cuts at: 4097 4127 1178
Size: 30 4105
Fragments arranged by size:
4105 1944 372 318 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2404 2437 3002 1186
Size: 1218 33 565 5238
Fragments arranged by size:
5238 1218 565 33
BseYI C'CCAG_C
Cuts at: 2142 2828 3022 3925 4045 6257 6753 2142
Size: 686 194 903 120 2212 496 2443
Fragments arranged by size:
2443 2212 903 686 496 194 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2132 2262 2274 2582 2777 3277 3559
Size: 573 396 130 12 308 195 500 282
Cuts at: 3559 3953 4283 4307 1163
Size: 394 330 24 3910
Fragments arranged by size:
3910 573 500 396 394 330 308 282
195 130 24 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2641 3916 4504 5265 6293 1540
Size: 275 186 640 1275 588 761 1028 2301
Fragments arranged by size:
2301 1275 1028 761 640 588 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2471 3957 5115 5200 6271 2211
Size: 260 1486 1158 85 1071 2994
Fragments arranged by size:
2994 1486 1158 1071 260 85
BsmI GAATG_Cn'
Cuts at: 4565 4658 4565
Size: 93 6961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2400 2471 2483 2805 2855 3932
Size: 399 421 189 71 12 322 50 1077
Cuts at: 3932 3957 4226 4373 5115 5200 6271 1391
Size: 25 269 147 742 85 1071 2174
Fragments arranged by size:
2174 1077 1071 742 421 399 322 269
189 147 85 71 50 25 12
BspEI T'CCGG_A
Cuts at: 6717 6717
Size: 7054
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 3905 4279 4409 844
Size: 1301 1760 374 130 3489
Fragments arranged by size:
3489 1760 1301 374 130
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 4941 1394
Size: 429 326 2792 3507
Fragments arranged by size:
3507 2792 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 4357 5549 5723 65
Size: 1183 3109 1192 174 1396
Fragments arranged by size:
3109 1396 1192 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2541 2994 3978 5698 5885 1700
Size: 110 731 453 984 1720 187 2869
Fragments arranged by size:
2869 1720 984 731 453 187 110
BsrGI T'GTAC_A
Cuts at: 96 2316 96
Size: 2220 4834
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 3008 5114 6126 3008
Size: 2106 1012 3936
Fragments arranged by size:
3936 2106 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2137 2946 794
Size: 1343 809 4902
Fragments arranged by size:
4902 1343 809
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 4291 1184
Size: 3107 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 4078 4078
Size: 7054
BstYI r'GATC_y
Cuts at: 1972 3138 3378 4384 4751 5136 5153 6605 6691
Size: 1166 240 1006 367 385 17 1452 86
Cuts at: 6691 6703 7049 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1166 1006 385 367 346 240
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2391 2515 2581 2610 3633 4158
Size: 552 939 387 124 66 29 1023 525
Cuts at: 4158 513
Size: 3409
Fragments arranged by size:
3409 1023 939 552 525 387 124 66
29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2089 2732 487
Size: 683 56 863 643 4809
Fragments arranged by size:
4809 863 683 643 56
BtsI GCAGTG_nn'
Cuts at: 2492 2684 3289 3428 4105 4570 5364 5384 2492
Size: 192 605 139 677 465 794 20 4162
Fragments arranged by size:
4162 794 677 605 465 192 139 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7054
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7019
DraI TTT'AAA
Cuts at: 4358 4358
Size: 7054
DraIII CAC_nnn'GTG
Cuts at: 3013 3013
Size: 7054
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6061 809
Size: 507 4745 1802
Fragments arranged by size:
4745 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2619 4014 4334
Size: 54 1425 50 275 18 789 1395 320
Cuts at: 4334 4501 5390 7019 8
Size: 167 889 1629 43
Fragments arranged by size:
1629 1425 1395 889 789 320 275 167
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4501 1537
Size: 275 2689 4090
Fragments arranged by size:
4090 2689 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 3778 4984 1353
Size: 654 1771 1206 3423
Fragments arranged by size:
3423 1771 1206 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2304 5641 6015 6161 387
Size: 1064 138 715 3337 374 146 1280
Fragments arranged by size:
3337 1280 1064 715 374 146 138
EcoICRI GAG'CTC
Cuts at: 2469 2469
Size: 7054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2225 3005 3359 3461 3584 3625
Size: 393 407 186 780 354 102 123 41
Cuts at: 3625 3671 4219 5117 5714 6501 6769 1239
Size: 46 548 898 597 787 268 1524
Fragments arranged by size:
1524 898 787 780 597 548 407 393
354 268 186 123 102 46 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2987 2987
Size: 7054
EcoO109I rG'GnC_Cy
Cuts at: 2343 2732 4222 4223 2343
Size: 389 1490 1 5174
Fragments arranged by size:
5174 1490 389 1
EcoRI G'AATT_C
Cuts at: 4363 4741 4363
Size: 378 6676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 7054
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 2187 2826 3438 6201 1678
Size: 509 639 612 2763 2531
Fragments arranged by size:
2763 2531 639 612 509
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3833 3865 5695 5727 5769
Size: 32 135 32 2261 32 1830 32 42
Cuts at: 5769 5801 1373
Size: 32 2626
Fragments arranged by size:
2626 2261 1830 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4398 4644 669
Size: 988 281 2460 246 3079
Fragments arranged by size:
3079 2460 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3762 748
Size: 3014 4040
HpaI GTT'AAC
Cuts at: 4644 4644
Size: 7054
KasI G'GCGC_C
Cuts at: 2183 2822 3434 2183
Size: 639 612 5803
Fragments arranged by size:
5803 639 612
KpnI G_GTAC'C
Cuts at: 2572 4379 2572
Size: 1807 5247
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6168 6352 1305
Size: 4863 184 2007
Fragments arranged by size:
4863 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 4016 10
Size: 54 1425 343 789 1395 3048
Fragments arranged by size:
3048 1425 1395 789 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 3236 3524 4076 5012 5371 5530
Size: 648 942 1128 288 552 936 359 159
Cuts at: 5530 518
Size: 2042
Fragments arranged by size:
2042 1128 942 936 648 552 359 288
159
MspA1I CmG'CkG
Cuts at: 1606 2195 2258 2334 2517 2566 2583 2612 2632
Size: 589 63 76 183 49 17 29 20
Cuts at: 2632 2832 3022 3490 3511 3562 3935 4095 4470
Size: 200 190 468 21 51 373 160 375
Cuts at: 4470 5147 6295 6540 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 468 375 373 245
200 190 183 160 76 63 51 49
29 21 20 17
NaeI GCC'GGC
Cuts at: 2996 2996
Size: 7054
NarI GG'CG_CC
Cuts at: 2184 2823 3435 2184
Size: 639 612 5803
Fragments arranged by size:
5803 639 612
NcoI C'CATG_G
Cuts at: 513 1065 2004 2391 513
Size: 552 939 387 5176
Fragments arranged by size:
5176 939 552 387
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7054
NgoMIV G'CCGG_C
Cuts at: 2994 2994
Size: 7054
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 2944 4412 5957 1132
Size: 97 1715 1468 1545 2229
Fragments arranged by size:
2229 1715 1545 1468 97
PasI CC'CwG_GG
Cuts at: 1348 2818 1348
Size: 1470 5584
PciI A'CATG_T
Cuts at: 5953 5953
Size: 7054
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3511 1108
Size: 2403 4651
PfoI T'CCnGG_A
Cuts at: 3141 3648 3681 3141
Size: 507 33 6514
Fragments arranged by size:
6514 507 33
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PmlI CAC'GTG
Cuts at: 2478 2910 3973 4243 2478
Size: 432 1063 270 5289
Fragments arranged by size:
5289 1063 432 270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 5123 5155 6636 6668 3347
Size: 32 1744 32 1481 32 3733
Fragments arranged by size:
3733 1744 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2343 2732 2343
Size: 389 6665
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 4624 4624
Size: 7054
PspOMI G'GGCC_C
Cuts at: 2801 4222 2801
Size: 1421 5633
PspXI vC'TCGA_Gb
Cuts at: 2549 2549
Size: 7054
PstI C_TGCA'G
Cuts at: 830 2178 2608 3498 3561 3813 3902 4406 830
Size: 1348 430 890 63 252 89 504 3478
Fragments arranged by size:
3478 1348 890 504 430 252 89 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
PvuII CAG'CTG
Cuts at: 2258 2632 3511 3562 3935 4095 2258
Size: 374 879 51 373 160 5217
Fragments arranged by size:
5217 879 374 373 160 51
SacI G_AGCT'C
Cuts at: 2471 2471
Size: 7054
SacII CC_GC'GG
Cuts at: 2518 2584 2613 2518
Size: 66 29 6959
Fragments arranged by size:
6959 66 29
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SanDI GG'GwC_CC
Cuts at: 2732 2732
Size: 7054
SapI GCTCTTCn'nnn_
Cuts at: 2007 2007
Size: 7054
SbfI CC_TGCA'GG
Cuts at: 4406 4406
Size: 7054
ScaI AGT'ACT
Cuts at: 3332 5302 7014 3332
Size: 1970 1712 3372
Fragments arranged by size:
3372 1970 1712
SfcI C'TryA_G
Cuts at: 826 1045 2174 2604 2935 3494 3557 3809 3898
Size: 219 1129 430 331 559 63 252 89
Cuts at: 3898 4402 5537 6218 6409 826
Size: 504 1135 681 191 1471
Fragments arranged by size:
1471 1135 1129 681 559 504 430 331
252 219 191 89 63
SfoI GGC'GCC
Cuts at: 2185 2824 3436 2185
Size: 639 612 5803
Fragments arranged by size:
5803 639 612
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 2162 2729 2856 4381 1872
Size: 290 567 127 1525 4545
Fragments arranged by size:
4545 1525 567 290 127
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 2549 2711 5158 6059 6321
Size: 197 926 417 189 162 2447 901 262
Cuts at: 6321 820
Size: 1553
Fragments arranged by size:
2447 1553 926 901 417 262 197 189
162
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 4412 1132
Size: 3280 3774
SspI AAT'ATT
Cuts at: 5 52 4978 5
Size: 47 4926 2081
Fragments arranged by size:
4926 2081 47
StuI AGG'CCT
Cuts at: 1517 3065 4064 1517
Size: 1548 999 4507
Fragments arranged by size:
4507 1548 999
StyI C'CwwG_G
Cuts at: 513 1065 2004 2391 3234 3945 4493 513
Size: 552 939 387 843 711 548 3074
Fragments arranged by size:
3074 939 843 711 552 548 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 4008 5081 5098 5251 5436 5881
Size: 1255 2058 370 1073 17 153 185 445
Cuts at: 5881 325
Size: 1498
Fragments arranged by size:
2058 1498 1255 1073 445 370 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 2943 3330
Size: 274 80 33 51 1443 339 627 387
Cuts at: 3330 5300 7012 96
Size: 1970 1712 138
Fragments arranged by size:
1970 1712 1443 627 387 339 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 4097 4120 4129 4152 4299 4331 7014
Size: 32 370 23 9 23 147 32 2683
Cuts at: 7014 7046 3695
Size: 32 3703
Fragments arranged by size:
3703 2683 370 147 32 32 32 23
23 9
Tth111I GACn'n_nGTC
Cuts at: 1316 4250 1316
Size: 2934 4120
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2749 3322 3508 4024 2749
Size: 573 186 516 5779
Fragments arranged by size:
5779 573 516 186
XhoI C'TCGA_G
Cuts at: 1943 2549 1943
Size: 606 6448
XmaI C'CCGG_G
Cuts at: 1870 2160 2727 2854 4379 1870
Size: 290 567 127 1525 4545
Fragments arranged by size:
4545 1525 567 290 127
XmnI GAAnn'nnTTC
Cuts at: 5183 5183
Size: 7054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2601 2686 4862 276
Size: 53 83 186 936 1067 85 2176 2468
Fragments arranged by size:
2468 2176 1067 936 186 85 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI
RsrII SexAI SfiI SrfI SwaI