Restriction map for
pFN21AE3949
(Circular) (Six-base) MAPSORT of: pFN21AE3949.seq Check: 5574 from: 1 to: 6913
pFN21AE3949 6913 bp
With 182 enzymes: SgfI *
March 28, 2012 11:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2574 2574
Size: 6913
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4723 278
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4256 1937
Size: 2319 4594
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 2823 3931 4976 6360 6628 1632
Size: 1085 106 1108 1045 1384 268 1917
Fragments arranged by size:
1917 1384 1108 1085 1045 268 106
AfeI AGC'GCT
Cuts at: 2358 3137 2358
Size: 779 6134
AflII C'TTAA_G
Cuts at: 820 1017 3551 820
Size: 197 2534 4182
Fragments arranged by size:
4182 2534 197
AflIII A'CryG_T
Cuts at: 1203 4023 5812 6789 1203
Size: 2820 1789 977 1327
Fragments arranged by size:
2820 1789 1327 977
AgeI A'CCGG_T
Cuts at: 5744 5744
Size: 6913
AhdI GACnn_n'nnGTC
Cuts at: 5642 5642
Size: 6913
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3781 1184
Size: 2597 4316
AlwNI CAG_nnn'CTG
Cuts at: 2875 4656 6228 2875
Size: 1781 1572 3560
Fragments arranged by size:
3560 1781 1572
ApaI G_GGCC'C
Cuts at: 2293 2408 2293
Size: 115 6798
ApaLI G'TGCA_C
Cuts at: 3752 4970 6126 3752
Size: 1218 1156 4539
Fragments arranged by size:
4539 1218 1156
ApoI r'AATT_y
Cuts at: 1493 4222 4448 4600 6709 1493
Size: 2729 226 152 2109 1697
Fragments arranged by size:
2729 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5467 160
Size: 872 4435 1606
Fragments arranged by size:
4435 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2092 2285 2408 3570 3912 3990
Size: 73 110 39 193 123 1162 342 78
Cuts at: 3990 4194 4238 1870
Size: 204 44 4545
Fragments arranged by size:
4545 1162 342 204 193 123 110 78
73 44 39
BamHI G'GATC_C
Cuts at: 3211 4243 4610 3211
Size: 1032 367 5514
Fragments arranged by size:
5514 1032 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2164 2261 2688 3933
Size: 325 217 627 311 66 97 427 1245
Cuts at: 3933 4030 4158 4189 4234 5689 618
Size: 97 128 31 45 1455 1842
Fragments arranged by size:
1842 1455 1245 627 427 325 311 217
128 97 97 66 45 31
BanII G_rGCy'C
Cuts at: 1391 2070 2293 2301 2408 2472 3070 3306 3543
Size: 679 223 8 107 64 598 236 237
Cuts at: 3543 3807 3891 4146 4232 1391
Size: 264 84 255 86 4072
Fragments arranged by size:
4072 679 598 264 255 237 236 223
107 86 84 64 8
BbeI G_GCGC'C
Cuts at: 2102 2168 2265 2692 3937 2102
Size: 66 97 427 1245 5078
Fragments arranged by size:
5078 1245 427 97 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5942
BbvCI CC'TCA_GC
Cuts at: 2960 2960
Size: 6913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5104 5138 1903
Size: 34 50 34 3083 34 3678
Fragments arranged by size:
3678 3083 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4805 6015 4805
Size: 1210 5703
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6913
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 2412 2835
Size: 107 122 71 631 605 478 262 423
Cuts at: 2835 3065 5524 136
Size: 230 2459 1525
Fragments arranged by size:
2459 1525 631 605 478 423 262 230
122 107 71
BglII A'GATC_T
Cuts at: 6908 6908
Size: 6913
BlpI GC'TnA_GC
Cuts at: 3600 4330 3600
Size: 730 6183
Bme1580I G_kGCm'C
Cuts at: 1790 2293 2408 3756 4009 4033 4192 4974 6130
Size: 503 115 1348 253 24 159 782 1156
Cuts at: 6130 1790
Size: 2573
Fragments arranged by size:
2573 1348 1156 782 503 253 159 115
24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6913
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2377 2662 2693
Size: 416 53 83 399 24 960 285 31
Cuts at: 2693 3743 4644 5602 442
Size: 1050 901 958 1753
Fragments arranged by size:
1753 1050 960 958 901 416 399 285
83 53 31 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 3281 2320
Size: 961 5952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 2696 3371 3498 3632 5573 1239
Size: 1418 39 675 127 134 1941 2579
Fragments arranged by size:
2579 1941 1418 675 134 127 39
Bpu10I CC'TnA_GC
Cuts at: 2064 2960 2985 3459 4035 2064
Size: 896 25 474 576 4942
Fragments arranged by size:
4942 896 576 474 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5038 5903 6201 5038
Size: 865 298 5750
Fragments arranged by size:
5750 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3097 5576 882
Size: 617 1598 2479 2219
Fragments arranged by size:
2479 2219 1598 617
BsaAI yAC'GTr
Cuts at: 493 1204 4026 4704 493
Size: 711 2822 678 2702
Fragments arranged by size:
2822 2702 711 678
BsaBI GATnn'nnATC
Cuts at: 6907 6907
Size: 6913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 2165 2262 2451
Size: 53 83 186 936 566 66 97 189
Cuts at: 2451 2642 2689 3934 4720 5102 5737 275
Size: 191 47 1245 786 382 635 1451
Fragments arranged by size:
1451 1245 936 786 635 566 382 191
189 186 97 83 66 53 47
BsaWI w'CCGG_w
Cuts at: 3953 5346 5744 6018 6165 6576 3953
Size: 1393 398 274 147 411 4290
Fragments arranged by size:
4290 1393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2331 2361 4037 4067 1178
Size: 30 165 30 928 30 1676 30 4024
Fragments arranged by size:
4024 1676 928 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2006 2738 2801 2825 3830 4106 4109 4220
Size: 820 732 63 24 1005 276 3 111
Cuts at: 4220 1186
Size: 3879
Fragments arranged by size:
3879 1005 820 732 276 111 63 24
3
BseYI C'CCAG_C
Cuts at: 2082 2212 2401 3087 3091 3427 3470 3575 3808
Size: 130 189 686 4 336 43 105 233
Cuts at: 3808 4121 6116 6612 2082
Size: 313 1995 496 2383
Fragments arranged by size:
2383 1995 686 496 336 313 233 189
130 105 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2373 1163
Size: 573 637 5703
Fragments arranged by size:
5703 637 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2024 2154 2172 2253 4363 5124
Size: 275 186 23 130 18 81 2110 761
Cuts at: 5124 6152 1540
Size: 1028 2301
Fragments arranged by size:
2301 2110 1028 761 275 186 130 81
23 18
BsiHKAI G_wGCw'C
Cuts at: 3192 3756 4041 4974 5059 6130 3192
Size: 564 285 933 85 1071 3975
Fragments arranged by size:
3975 1071 933 564 285 85
BsmI GAATG_Cn'
Cuts at: 4424 4517 4424
Size: 93 6820
BsmBI CGTCTCn'nnnn_
Cuts at: 2633 2633
Size: 6913
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2293 2301 2408 2472 3070 3192
Size: 399 280 223 8 107 64 598 122
Cuts at: 3192 3306 3543 3756 3807 3891 4009 4033 4041
Size: 114 237 213 51 84 118 24 8
Cuts at: 4041 4146 4192 4232 4974 5059 6130 1391
Size: 105 46 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 598 399 280 237 223
213 122 118 114 107 105 85 84
64 51 46 40 24 8 8
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 4802 6532 4802
Size: 1730 5183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2490 2574 4268 844
Size: 1646 84 1694 3489
Fragments arranged by size:
3489 1694 1646 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3400 3507 3625 4800 1394
Size: 429 1577 107 118 1175 3507
Fragments arranged by size:
3507 1577 1175 429 118 107
BsrDI GCAATG_nn'
Cuts at: 65 1248 2850 5408 5582 65
Size: 1183 1602 2558 174 1396
Fragments arranged by size:
2558 1602 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 2883 5557 5744 1700
Size: 110 357 716 2674 187 2869
Fragments arranged by size:
2869 2674 716 357 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6913
BssHII G'CGCG_C
Cuts at: 1925 2140 2439 1925
Size: 215 299 6399
Fragments arranged by size:
6399 299 215
BssSI C'ACGA_G
Cuts at: 4973 5985 4973
Size: 1012 5901
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6913
BstBI TT'CG_AA
Cuts at: 5717 5717
Size: 6913
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6913
BstYI r'GATC_y
Cuts at: 1972 2515 3211 4243 4610 4995 5012 6464 6550
Size: 543 696 1032 367 385 17 1452 86
Cuts at: 6550 6562 6908 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1032 696 543 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2784 3246 2784
Size: 462 6451
BtgI C'CryG_G
Cuts at: 513 1065 2172 3183 3970 513
Size: 552 1107 1011 787 3456
Fragments arranged by size:
3456 1107 1011 787 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6174
Fragments arranged by size:
6174 683 56
BtsI GCAGTG_nn'
Cuts at: 2708 2885 4429 5223 5243 2708
Size: 177 1544 794 20 4378
Fragments arranged by size:
4378 1544 794 177 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6878
DraI TTT'AAA
Cuts at: 4217 4217
Size: 6913
DraIII CAC_nnn'GTG
Cuts at: 3694 3751 3694
Size: 57 6856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2086 3767 5920 809
Size: 507 770 1681 2153 1802
Fragments arranged by size:
2153 1802 1681 770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2021 2151 2169
Size: 54 1425 50 275 18 191 130 18
Cuts at: 2169 2250 4360 5249 6878 8
Size: 81 2110 889 1629 43
Fragments arranged by size:
2110 1629 1425 889 275 191 130 81
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2021 2151 2169 2250 4360 1537
Size: 275 209 130 18 81 2110 4090
Fragments arranged by size:
4090 2110 275 209 130 81 18
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 2802 3041 3158 3278 4095 4101 4843
Size: 1383 66 239 117 120 817 6 742
Cuts at: 4843 1353
Size: 3423
Fragments arranged by size:
3423 1383 817 742 239 120 117 66
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2351 2447 5500 5874 6020 387
Size: 1064 138 762 96 3053 374 146 1280
Fragments arranged by size:
3053 1280 1064 762 374 146 138 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2657 2696 2717 2823 3371 3498 3632
Size: 393 1025 39 21 106 548 127 134
Cuts at: 3632 3931 4976 5573 6360 6628 1239
Size: 299 1045 597 787 268 1524
Fragments arranged by size:
1524 1045 1025 787 597 548 393 299
268 134 127 106 39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2843 2843
Size: 6913
EcoO109I rG'GnC_Cy
Cuts at: 2058 2110 2289 2290 2315 2328 2405 2490 2632
Size: 52 179 1 25 13 77 85 142
Cuts at: 2632 2780 2836 2872 3871 2058
Size: 148 56 36 999 5100
Fragments arranged by size:
5100 999 179 148 142 85 77 56
52 36 25 13 1
EcoRI G'AATT_C
Cuts at: 4222 4600 4222
Size: 378 6535
FspI TGC'GCA
Cuts at: 2389 3561 5419 2389
Size: 1172 1858 3883
Fragments arranged by size:
3883 1858 1172
HaeII r_GCGC'y
Cuts at: 1678 2102 2168 2180 2195 2213 2265 2360 2692
Size: 424 66 12 15 18 52 95 332
Cuts at: 2692 3139 3708 3937 4003 6060 1678
Size: 447 569 229 66 2057 2531
Fragments arranged by size:
2531 2057 569 447 424 332 229 95
66 66 52 18 15 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2331 2363 3096 3114 3128
Size: 32 135 32 759 32 733 18 14
Cuts at: 3128 3146 5554 5586 5628 5660 1373
Size: 18 2408 32 42 32 2626
Fragments arranged by size:
2626 2408 759 733 135 42 32 32
32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4257 4503 669
Size: 988 281 2319 246 3079
Fragments arranged by size:
3079 2319 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6913
HpaI GTT'AAC
Cuts at: 4503 4503
Size: 6913
KasI G'GCGC_C
Cuts at: 2098 2164 2261 2688 3933 2098
Size: 66 97 427 1245 5078
Fragments arranged by size:
5078 1245 427 97 66
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 4512 4512
Size: 6913
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3375 3390 6027 6211 1305
Size: 2070 15 2637 184 2007
Fragments arranged by size:
2637 2070 2007 184 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5091
Fragments arranged by size:
5091 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4871 5230 5389 518
Size: 648 3705 359 159 2042
Fragments arranged by size:
3705 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2086 2525 2959 3091 3385 3454 3470
Size: 420 60 439 434 132 294 69 16
Cuts at: 3470 3565 3834 4329 5006 6154 6399 1606
Size: 95 269 495 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 495 439 434 420 294
269 245 132 95 69 60 16
NaeI GCC'GGC
Cuts at: 2169 2885 2169
Size: 716 6197
NarI GG'CG_CC
Cuts at: 2099 2165 2262 2689 3934 2099
Size: 66 97 427 1245 5078
Fragments arranged by size:
5078 1245 427 97 66
NcoI C'CATG_G
Cuts at: 513 1065 3183 3970 513
Size: 552 2118 787 3456
Fragments arranged by size:
3456 2118 787 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6913
NgoMIV G'CCGG_C
Cuts at: 2167 2883 2167
Size: 716 6197
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NspI r_CATG'y
Cuts at: 1132 1229 4271 5816 1132
Size: 97 3042 1545 2229
Fragments arranged by size:
3042 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3154 1348
Size: 1806 5107
PciI A'CATG_T
Cuts at: 5812 5812
Size: 6913
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6913
PfoI T'CCnGG_A
Cuts at: 2445 2628 3348 3771 2445
Size: 183 720 423 5587
Fragments arranged by size:
5587 720 423 183
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PmlI CAC'GTG
Cuts at: 4026 4026
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 6495 6527 4982
Size: 32 1481 32 5368
Fragments arranged by size:
5368 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2315 2328 2632 2872 3871 2058
Size: 257 13 304 240 999 5100
Fragments arranged by size:
5100 999 304 257 240 13
PshAI GACnn'nnGTC
Cuts at: 1613 2602 3741 1613
Size: 989 1139 4785
Fragments arranged by size:
4785 1139 989
PsiI TTA'TAA
Cuts at: 4483 4483
Size: 6913
PspOMI G'GGCC_C
Cuts at: 2289 2404 2289
Size: 115 6798
PstI C_TGCA'G
Cuts at: 830 2487 2571 2880 3235 3453 4080 4265 830
Size: 1657 84 309 355 218 627 185 3478
Fragments arranged by size:
3478 1657 627 355 309 218 185 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6913
PvuII CAG'CTG
Cuts at: 2086 2525 2959 3091 3385 3454 3470 3565 2086
Size: 439 434 132 294 69 16 95 5434
Fragments arranged by size:
5434 439 434 294 132 95 69 16
SalI G'TCGA_C
Cuts at: 1936 4255 1936
Size: 2319 4594
SanDI GG'GwC_CC
Cuts at: 2058 2315 2058
Size: 257 6656
SapI GCTCTTCn'nnn_
Cuts at: 2736 3041 2736
Size: 305 6608
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 5161 6873 5161
Size: 1712 5201
SexAI A'CCwGG_T
Cuts at: 3690 3690
Size: 6913
SfcI C'TryA_G
Cuts at: 826 1045 2483 2567 2876 3009 3231 3449 3829
Size: 219 1438 84 309 133 222 218 380
Cuts at: 3829 4076 4261 5396 6077 6268 826
Size: 247 185 1135 681 191 1471
Fragments arranged by size:
1471 1438 1135 681 380 309 247 222
219 218 191 185 133 84
SfoI GGC'GCC
Cuts at: 2100 2166 2263 2690 3935 2100
Size: 66 97 427 1245 5078
Fragments arranged by size:
5078 1245 427 97 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6913
SmaI CCC'GGG
Cuts at: 1872 2055 2094 2410 3572 4240 1872
Size: 183 39 316 1162 668 4545
Fragments arranged by size:
4545 1162 668 316 183 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3551 5017 5918 6180 820
Size: 197 926 1608 1466 901 262 1553
Fragments arranged by size:
1608 1553 1466 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4704 493
Size: 4211 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 4271 1132
Size: 3139 3774
SrfI GCCC'GGGC
Cuts at: 3572 3572
Size: 6913
SspI AAT'ATT
Cuts at: 5 52 4837 5
Size: 47 4785 2081
Fragments arranged by size:
4785 2081 47
StuI AGG'CCT
Cuts at: 1517 3289 3647 1517
Size: 1772 358 4783
Fragments arranged by size:
4783 1772 358
StyI C'CwwG_G
Cuts at: 513 1065 3183 3290 3970 4352 513
Size: 552 2118 107 680 382 3074
Fragments arranged by size:
3074 2118 680 552 382 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3701 3762 4940 4957 5110 5295 5740
Size: 1255 2121 61 1178 17 153 185 445
Cuts at: 5740 325
Size: 1498
Fragments arranged by size:
2121 1498 1255 1178 445 185 153 61
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2592 5159 6871
Size: 274 80 33 51 1443 615 2567 1712
Cuts at: 6871 96
Size: 138
Fragments arranged by size:
2567 1712 1443 615 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4037 4069 6873 6905 4037
Size: 32 2804 32 4045
Fragments arranged by size:
4045 2804 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6913
XbaI T'CTAG_A
Cuts at: 4249 4249
Size: 6913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3190 4178 3190
Size: 988 5925
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 2053 2092 2408 3570 4238 1870
Size: 183 39 316 1162 668 4545
Fragments arranged by size:
4545 1162 668 316 183 39
XmnI GAAnn'nnTTC
Cuts at: 5042 5042
Size: 6913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4721 276
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacI SacII SfiI SwaI