Restriction map for
pFN21AE3947
(Circular) (Six-base) MAPSORT of: pFN21AE3947.seq Check: 6141 from: 1 to: 7999
pFN21AE3947 7999 bp
With 182 enzymes: SgfI *
March 28, 2012 11:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3911 3951 3911
Size: 40 7959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5809 278
Size: 53 83 186 936 4273 2468
Fragments arranged by size:
4273 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5211 5342 1937
Size: 3274 131 4594
Fragments arranged by size:
4594 3274 131
Acc65I G'GTAC_C
Cuts at: 5320 5320
Size: 7999
AclI AA'CG_TT
Cuts at: 1563 6126 6499 1563
Size: 4563 373 3063
Fragments arranged by size:
4563 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3408 4881 6062 7446 7714 1632
Size: 1776 1473 1181 1384 268 1917
Fragments arranged by size:
1917 1776 1473 1384 1181 268
AfeI AGC'GCT
Cuts at: 4687 4687
Size: 7999
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7802
AflIII A'CryG_T
Cuts at: 1203 4450 5034 6898 7875 1203
Size: 3247 584 1864 977 1327
Fragments arranged by size:
3247 1864 1327 977 584
AgeI A'CCGG_T
Cuts at: 6830 6830
Size: 7999
AhdI GACnn_n'nnGTC
Cuts at: 6728 6728
Size: 7999
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7999
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7999
AlwNI CAG_nnn'CTG
Cuts at: 2243 3562 4213 5742 7314 2243
Size: 1319 651 1529 1572 2928
Fragments arranged by size:
2928 1572 1529 1319 651
ApaLI G'TGCA_C
Cuts at: 3081 6056 7212 3081
Size: 2975 1156 3868
Fragments arranged by size:
3868 2975 1156
ApoI r'AATT_y
Cuts at: 1493 2395 2820 3119 4260 4630 5308 5534 5686
Size: 902 425 299 1141 370 678 226 152
Cuts at: 5686 7795 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1141 902 678 425 370 299
226 152
AseI AT'TA_AT
Cuts at: 160 1032 3482 4500 6553 160
Size: 872 2450 1018 2053 1606
Fragments arranged by size:
2450 2053 1606 1018 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7999
AvaI C'yCGr_G
Cuts at: 1870 1943 5324 1870
Size: 73 3381 4545
Fragments arranged by size:
4545 3381 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4799 4832 4799
Size: 33 7966
BamHI G'GATC_C
Cuts at: 2118 5329 5696 2118
Size: 3211 367 4421
Fragments arranged by size:
4421 3211 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4015 5320 6775 618
Size: 325 217 627 2228 1305 1455 1842
Fragments arranged by size:
2228 1842 1455 1305 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4198 4254 5318 1391
Size: 2807 56 1064 4072
Fragments arranged by size:
4072 2807 1064 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2811 928
Size: 971 912 6116
Fragments arranged by size:
6116 971 912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6190 6224 1903
Size: 34 50 34 4169 34 3678
Fragments arranged by size:
4169 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2932 5891 7101 2932
Size: 2959 1210 3830
Fragments arranged by size:
3830 2959 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7999
BfrBI ATG'CAT
Cuts at: 2608 2608
Size: 7999
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6610 136
Size: 107 122 71 631 605 4938 1525
Fragments arranged by size:
4938 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2017 2201 3810 4327 7994 2017
Size: 184 1609 517 3667 2022
Fragments arranged by size:
3667 2022 1609 517 184
BlpI GC'TnA_GC
Cuts at: 2256 5416 2256
Size: 3160 4839
Bme1580I G_kGCm'C
Cuts at: 1790 3085 3601 6060 7216 1790
Size: 1295 516 2459 1156 2573
Fragments arranged by size:
2573 2459 1295 1156 516
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7999
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5730 6688 442
Size: 416 53 83 399 24 4313 958 1753
Fragments arranged by size:
4313 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2130 2226 4644 6659 1239
Size: 891 96 2418 2015 2579
Fragments arranged by size:
2579 2418 2015 891 96
Bpu10I CC'TnA_GC
Cuts at: 3474 3758 4253 3474
Size: 284 495 7220
Fragments arranged by size:
7220 495 284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 6124 6989 7287 2599
Size: 3525 865 298 3311
Fragments arranged by size:
3525 3311 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6662 882
Size: 617 5163 2219
Fragments arranged by size:
5163 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4055 5790 493
Size: 711 2851 1735 2702
Fragments arranged by size:
2851 2702 1735 711
BsaBI GATnn'nnATC
Cuts at: 7993 7993
Size: 7999
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2299 2582 3585 3954
Size: 53 83 186 936 766 283 1003 369
Cuts at: 3954 5806 6188 6823 275
Size: 1852 382 635 1451
Fragments arranged by size:
1852 1451 1003 936 766 635 382 369
283 186 83 53
BsaWI w'CCGG_w
Cuts at: 5196 6432 6830 7104 7251 7662 5196
Size: 1236 398 274 147 411 5533
Fragments arranged by size:
5533 1236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2007 2037 2067 2097 1178
Size: 30 165 30 604 30 30 30 7080
Fragments arranged by size:
7080 604 165 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3593 5120 1186
Size: 2407 1527 4065
Fragments arranged by size:
4065 2407 1527
BseYI C'CCAG_C
Cuts at: 7202 7698 7202
Size: 496 7503
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 5183 1163
Size: 573 482 2965 3979
Fragments arranged by size:
3979 2965 573 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5449 6210 7238 1540
Size: 275 186 3448 761 1028 2301
Fragments arranged by size:
3448 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3085 4254 6060 6145 7216 3085
Size: 1169 1806 85 1071 3868
Fragments arranged by size:
3868 1806 1169 1071 85
BsmI GAATG_Cn'
Cuts at: 2516 5510 5603 2516
Size: 2994 93 4912
Fragments arranged by size:
4912 2994 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3085 3601 4198 4254 5318 6060 6145
Size: 399 1295 516 597 56 1064 742 85
Cuts at: 6145 7216 1391
Size: 1071 2174
Fragments arranged by size:
2174 1295 1071 1064 742 597 516 399
85 56
BspEI T'CCGG_A
Cuts at: 7662 7662
Size: 7999
BspHI T'CATG_A
Cuts at: 5254 5888 7618 5254
Size: 634 1730 5635
Fragments arranged by size:
5635 1730 634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 3054 3301 3911 3937 3951 5354 844
Size: 1381 829 247 610 26 14 1403 3489
Fragments arranged by size:
3489 1403 1381 829 610 247 26 14
BsrBI CCG'CTC
Cuts at: 1394 1823 5886 1394
Size: 429 4063 3507
Fragments arranged by size:
4063 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3224 3324 4075 4141 6494 6668 65
Size: 1183 1976 100 751 66 2353 174 1396
Fragments arranged by size:
2353 1976 1396 1183 751 174 100 66
BsrFI r'CCGG_y
Cuts at: 1700 1810 6643 6830 1700
Size: 110 4833 187 2869
Fragments arranged by size:
4833 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3108 3960 96
Size: 3012 852 4135
Fragments arranged by size:
4135 3012 852
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7999
BssSI C'ACGA_G
Cuts at: 6059 7071 6059
Size: 1012 6987
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2252 4930 794
Size: 1458 2678 3863
Fragments arranged by size:
3863 2678 1458
BstBI TT'CG_AA
Cuts at: 5125 6803 5125
Size: 1678 6321
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7999
BstXI CCAn_nnnn'nTGG
Cuts at: 4960 4960
Size: 7999
BstYI r'GATC_y
Cuts at: 1972 2017 2099 2118 2201 3810 4327 5329 5696
Size: 45 82 19 83 1609 517 1002 367
Cuts at: 5696 6081 6098 7550 7636 7648 7994 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1609 1452 1002 517 385 367 346
86 83 82 45 19 17 12
Bsu36I CC'TnA_GG
Cuts at: 3126 3871 4302 3126
Size: 745 431 6823
Fragments arranged by size:
6823 745 431
BtgI C'CryG_G
Cuts at: 513 1065 2226 4668 513
Size: 552 1161 2442 3844
Fragments arranged by size:
3844 2442 1161 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3777 4475 487
Size: 683 56 2551 698 4011
Fragments arranged by size:
4011 2551 698 683 56
BtsI GCAGTG_nn'
Cuts at: 2184 2881 3825 4371 4884 5515 6309 6329 2184
Size: 697 944 546 513 631 794 20 3854
Fragments arranged by size:
3854 944 794 697 631 546 513 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7999
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3417 3452 529
Size: 35 2853 35 5076
Fragments arranged by size:
5076 2853 35 35
DraI TTT'AAA
Cuts at: 2649 3028 4938 5303 2649
Size: 379 1910 365 5345
Fragments arranged by size:
5345 1910 379 365
DraIII CAC_nnn'GTG
Cuts at: 3269 4930 3269
Size: 1661 6338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7006 809
Size: 507 5690 1802
Fragments arranged by size:
5690 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2881 3564 5446
Size: 54 1425 50 275 18 1051 683 1882
Cuts at: 5446 6335 7964 8
Size: 889 1629 43
Fragments arranged by size:
1882 1629 1425 1051 889 683 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5446 1537
Size: 275 3634 4090
Fragments arranged by size:
4090 3634 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3612 4142 4827 4857 5929 1353
Size: 2259 530 685 30 1072 3423
Fragments arranged by size:
3423 2259 1072 685 530 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6586 6960 7106 387
Size: 1064 138 4997 374 146 1280
Fragments arranged by size:
4997 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4252 4252
Size: 7999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2130 2226 3408 4644 4881 6062 6659
Size: 393 498 96 1182 1236 237 1181 597
Cuts at: 6659 7446 7714 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1236 1182 1181 787 597 498 393
268 237 96
EcoRI G'AATT_C
Cuts at: 5308 5686 5308
Size: 378 7621
EcoRV GAT'ATC
Cuts at: 2766 3280 3886 2766
Size: 514 606 6879
Fragments arranged by size:
6879 606 514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2825 2909 5068 2825
Size: 84 2159 5756
Fragments arranged by size:
5756 2159 84
FspI TGC'GCA
Cuts at: 6505 6505
Size: 7999
HaeII r_GCGC'y
Cuts at: 1678 4689 4870 7146 1678
Size: 3011 181 2276 2531
Fragments arranged by size:
3011 2531 2276 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2103 2116 2135 4155
Size: 32 135 32 512 19 13 19 2020
Cuts at: 4155 4187 5249 5281 6640 6672 6714 6746 1373
Size: 32 1062 32 1359 32 42 32 2626
Fragments arranged by size:
2626 2020 1359 1062 512 135 42 32
32 32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3646 3952 5005 5343 5589 669
Size: 988 281 1708 306 1053 338 246 3079
Fragments arranged by size:
3079 1708 1053 988 338 306 281 246
HindIII A'AGCT_T
Cuts at: 748 2771 3737 748
Size: 2023 966 5010
Fragments arranged by size:
5010 2023 966
HpaI GTT'AAC
Cuts at: 5589 5589
Size: 7999
KpnI G_GTAC'C
Cuts at: 5324 5324
Size: 7999
MfeI C'AATT_G
Cuts at: 5598 5598
Size: 7999
MluI A'CGCG_T
Cuts at: 7875 7875
Size: 7999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2434 3029 3407 4797 7113 7297 1305
Size: 1129 595 378 1390 2316 184 2007
Fragments arranged by size:
2316 2007 1390 1129 595 378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2883 10
Size: 54 1425 343 1051 5126
Fragments arranged by size:
5126 1425 1051 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 2531 2751 4607 4746 4748 4877
Size: 648 895 470 220 1856 139 2 129
Cuts at: 4877 5957 6316 6475 518
Size: 1080 359 159 2042
Fragments arranged by size:
2042 1856 1080 895 648 470 359 220
159 139 129 2
MspA1I CmG'CkG
Cuts at: 1606 5415 6092 7240 7485 1606
Size: 3809 677 1148 245 2120
Fragments arranged by size:
3809 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2226 4668 513
Size: 552 1161 2442 3844
Fragments arranged by size:
3844 2442 1161 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7999
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7999
NotI GC'GGCC_GC
Cuts at: 5446 5446
Size: 7999
NsiI A_TGCA'T
Cuts at: 2610 2610
Size: 7999
NspI r_CATG'y
Cuts at: 1132 1229 2546 4351 4454 4606 5038 5357 6902
Size: 97 1317 1805 103 152 432 319 1545
Cuts at: 6902 1132
Size: 2229
Fragments arranged by size:
2229 1805 1545 1317 432 319 152 103
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7999
PciI A'CATG_T
Cuts at: 4450 5034 6898 4450
Size: 584 1864 5551
Fragments arranged by size:
5551 1864 584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2969 1108
Size: 1861 6138
PfoI T'CCnGG_A
Cuts at: 4212 4212
Size: 7999
PmeI GTTT'AAAC
Cuts at: 5303 5303
Size: 7999
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6068 6100 7581 7613 6068
Size: 32 1481 32 6454
Fragments arranged by size:
6454 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7999
PsiI TTA'TAA
Cuts at: 2700 5569 2700
Size: 2869 5130
PstI C_TGCA'G
Cuts at: 830 2052 3948 5351 830
Size: 1222 1896 1403 3478
Fragments arranged by size:
3478 1896 1403 1222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7999
SacI G_AGCT'C
Cuts at: 4254 4254
Size: 7999
SalI G'TCGA_C
Cuts at: 1936 5341 1936
Size: 3405 4594
SapI GCTCTTCn'nnn_
Cuts at: 4857 4857
Size: 7999
SbfI CC_TGCA'GG
Cuts at: 3948 5351 3948
Size: 1403 6596
ScaI AGT'ACT
Cuts at: 6247 7959 6247
Size: 1712 6287
SexAI A'CCwGG_T
Cuts at: 2968 2968
Size: 7999
SfcI C'TryA_G
Cuts at: 826 1045 2048 3944 4405 5347 6482 7163 7354
Size: 219 1003 1896 461 942 1135 681 191
Cuts at: 7354 826
Size: 1471
Fragments arranged by size:
1896 1471 1135 1003 942 681 461 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7999
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7999
SmaI CCC'GGG
Cuts at: 1872 5326 1872
Size: 3454 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2614 6103 7004 7266 820
Size: 197 926 671 3489 901 262 1553
Fragments arranged by size:
3489 1553 926 901 671 262 197
SnaBI TAC'GTA
Cuts at: 493 4055 5790 493
Size: 3562 1735 2702
Fragments arranged by size:
3562 2702 1735
SpeI A'CTAG_T
Cuts at: 152 2195 152
Size: 2043 5956
SphI G_CATG'C
Cuts at: 1132 5357 1132
Size: 4225 3774
SspI AAT'ATT
Cuts at: 5 52 3506 3985 5923 5
Size: 47 3454 479 1938 2081
Fragments arranged by size:
3454 2081 1938 479 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7999
StyI C'CwwG_G
Cuts at: 513 1065 2086 2226 4668 4796 5189 5438 513
Size: 552 1021 140 2442 128 393 249 3074
Fragments arranged by size:
3074 2442 1021 552 393 249 140 128
SwaI ATTT'AAAT
Cuts at: 2649 2649
Size: 7999
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4788 6026 6043 6196 6381 6826 325
Size: 1255 3208 1238 17 153 185 445 1498
Fragments arranged by size:
3208 1498 1255 1238 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3108 3960
Size: 274 80 33 51 1443 117 1014 852
Cuts at: 3960 5017 6245 7957 96
Size: 1057 1228 1712 138
Fragments arranged by size:
1712 1443 1228 1057 1014 852 274 138
117 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4701 4733 4904 4936 7959 7991 4701
Size: 32 171 32 3023 32 4709
Fragments arranged by size:
4709 3023 171 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2551 1316
Size: 1235 6764
XbaI T'CTAG_A
Cuts at: 2347 5335 2347
Size: 2988 5011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7999
XmaI C'CCGG_G
Cuts at: 1870 5324 1870
Size: 3454 4545
XmnI GAAnn'nnTTC
Cuts at: 6128 6128
Size: 7999
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5807 276
Size: 53 83 186 936 4273 2468
Fragments arranged by size:
4273 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI
BsmBI BstZ17I EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
XcmI