Restriction map for
pFN21AE3942
(Circular) (Six-base) MAPSORT of: pFN21AE3942.seq Check: 9404 from: 1 to: 6004
pFN21AE3942 6004 bp
With 182 enzymes: SgfI *
March 28, 2012 11:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2133 3243 3297 3347 1937
Size: 196 1110 54 50 4594
Fragments arranged by size:
4594 1110 196 54 50
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3012 3137 4067 5451 5719 1632
Size: 1380 125 930 1384 268 1917
Fragments arranged by size:
1917 1384 1380 930 268 125
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2231 4903 5880 1203
Size: 1028 2672 977 1327
Fragments arranged by size:
2672 1327 1028 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 2477
Size: 32 5972
AlwNI CAG_nnn'CTG
Cuts at: 3747 5319 3747
Size: 1572 4432
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 3313 3539 3691 5800 1493
Size: 1820 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 1870
Size: 73 1386 4545
Fragments arranged by size:
4545 1386 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2307 2865 2920 3325 4780
Size: 325 217 627 520 558 55 405 1455
Cuts at: 4780 618
Size: 1842
Fragments arranged by size:
1842 1455 627 558 520 405 325 217
55
BanII G_rGCy'C
Cuts at: 1391 2855 2932 3323 1391
Size: 1464 77 391 4072
Fragments arranged by size:
4072 1464 391 77
BbeI G_GCGC'C
Cuts at: 2869 2869
Size: 6004
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2156 2804 928
Size: 971 257 648 4128
Fragments arranged by size:
4128 971 648 257
BbvCI CC'TCA_GC
Cuts at: 2647 2647
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 2445 1548
Size: 897 5107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2475 3190
Size: 107 122 71 631 605 337 466 715
Cuts at: 3190 3273 4615 136
Size: 83 1342 1525
Fragments arranged by size:
1525 1342 715 631 605 466 337 122
107 83 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 2310 2329 2923 4065 5221 1790
Size: 520 19 594 1142 1156 2573
Fragments arranged by size:
2573 1156 1142 594 520 19
BmgBI CAC'GTC
Cuts at: 1318 2053 1318
Size: 735 5269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2858 3735 4693
Size: 416 53 83 399 24 1441 877 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1441 958 877 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2708 1056
Size: 1652 4352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3086
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2359 2654 2896 2946 3072 4664 1239
Size: 1120 295 242 50 126 1592 2579
Fragments arranged by size:
2579 1592 1120 295 242 126 50
Bpu10I CC'TnA_GC
Cuts at: 2647 2647
Size: 6004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2377 4667 882
Size: 617 878 2290 2219
Fragments arranged by size:
2290 2219 878 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2358 2866 3811 4193
Size: 53 83 186 936 825 508 945 382
Cuts at: 4193 4828 275
Size: 635 1451
Fragments arranged by size:
1451 945 936 825 635 508 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2223 2743 4437 4835 5109 5256 5667 2223
Size: 520 1694 398 274 147 411 2560
Fragments arranged by size:
2560 1694 520 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2869 2899 1178
Size: 30 165 30 597 30 839 30 4283
Fragments arranged by size:
4283 839 597 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3035 3038 3092 3095 3143 1186
Size: 1849 3 54 3 48 4047
Fragments arranged by size:
4047 1849 54 48 3 3
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2333 3199 1163
Size: 573 417 180 866 3968
Fragments arranged by size:
3968 866 573 417 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2932 4065 4150 5221 2932
Size: 1133 85 1071 3715
Fragments arranged by size:
3715 1133 1071 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2329 2855 2923 2932 3323 4065
Size: 399 520 19 526 68 9 391 742
Cuts at: 4065 4150 5221 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 520 399 391 85
68 19 9
BspEI T'CCGG_A
Cuts at: 2743 5667 2743
Size: 2924 3080
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 3359 844
Size: 1304 1211 3489
Fragments arranged by size:
3489 1304 1211
BsrBI CCG'CTC
Cuts at: 1394 1823 3133 3262 3891 1394
Size: 429 1310 129 629 3507
Fragments arranged by size:
3507 1310 629 429 129
BsrDI GCAATG_nn'
Cuts at: 65 1248 2195 2418 4499 4673 65
Size: 1183 947 223 2081 174 1396
Fragments arranged by size:
2081 1396 1183 947 223 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3189 4648 4835 1700
Size: 110 1379 1459 187 2869
Fragments arranged by size:
2869 1459 1379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6004
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 2939 1184
Size: 1755 4249
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2431
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999
Size: 1362 367 385 17 1452 86 12 346
Cuts at: 5999 1972
Size: 1977
Fragments arranged by size:
1977 1452 1362 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2756 2756
Size: 6004
BtgI C'CryG_G
Cuts at: 513 1065 2004 2179 2217 2595 513
Size: 552 939 175 38 378 3922
Fragments arranged by size:
3922 939 552 378 175 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2019 2327 2526 2590 2727 3520 4314 4334 2019
Size: 308 199 64 137 793 794 20 3689
Fragments arranged by size:
3689 794 793 308 199 137 64 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3236 3271 529
Size: 35 2672 35 3262
Fragments arranged by size:
3262 2672 35 35
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 2592 2598
Size: 54 1425 50 275 18 550 212 6
Cuts at: 2598 2664 2795 2903 3451 4340 5969 8
Size: 66 131 108 548 889 1629 43
Fragments arranged by size:
1629 1425 889 550 548 275 212 131
108 66 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2991 3012 3934 1353
Size: 1638 21 922 3423
Fragments arranged by size:
3423 1638 922 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2930 2930
Size: 6004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2359 2654 2896 2946 3012 3072 3137
Size: 393 727 295 242 50 66 60 65
Cuts at: 3137 4067 4664 5451 5719 1239
Size: 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 727 597 393 295 268
242 66 65 60 50
EcoNI CCTnn'n_nnAGG
Cuts at: 3206 3206
Size: 6004
EcoO109I rG'GnC_Cy
Cuts at: 2967 2967
Size: 6004
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2869 5151 1678
Size: 1191 2282 2531
Fragments arranged by size:
2531 2282 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 2808 2840 2869
Size: 32 135 32 943 32 261 32 29
Cuts at: 2869 2901 4645 4677 4719 4751 1373
Size: 32 1744 32 42 32 2626
Fragments arranged by size:
2626 1744 943 261 135 42 32 32
32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2461 3348 3594 669
Size: 988 281 523 887 246 3079
Fragments arranged by size:
3079 988 887 523 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KasI G'GCGC_C
Cuts at: 2865 2865
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3253 3603 3253
Size: 350 5654
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 5118 5302 1305
Size: 744 3069 184 2007
Fragments arranged by size:
3069 2007 744 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 2594 2600 2666 2905
Size: 54 1425 343 550 212 6 66 239
Cuts at: 2905 10
Size: 3109
Fragments arranged by size:
3109 1425 550 343 239 212 66 54
6
MslI CAynn'nnrTG
Cuts at: 518 1166 2423 3041 3962 4321 4480 518
Size: 648 1257 618 921 359 159 2042
Fragments arranged by size:
2042 1257 921 648 618 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 2651 2893 3420 4097 5245 5490 1606
Size: 575 470 242 527 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 575 527 470 245 242
NaeI GCC'GGC
Cuts at: 3191 3191
Size: 6004
NarI GG'CG_CC
Cuts at: 2866 2866
Size: 6004
NcoI C'CATG_G
Cuts at: 513 1065 2004 2595 513
Size: 552 939 591 3922
Fragments arranged by size:
3922 939 591 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NgoMIV G'CCGG_C
Cuts at: 3189 3189
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 2704 1052
Size: 1652 4352
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2235 3362 4907 1132
Size: 97 1006 1127 1545 2229
Fragments arranged by size:
2229 1545 1127 1006 97
PasI CC'CwG_GG
Cuts at: 1348 2935 1348
Size: 1587 4417
PciI A'CATG_T
Cuts at: 2231 4903 2231
Size: 2672 3332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2967 2967
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 3050 1613
Size: 1437 4567
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2892 3285 3356 830
Size: 2062 393 71 3478
Fragments arranged by size:
3478 2062 393 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2651 2893 2651
Size: 242 5762
SacI G_AGCT'C
Cuts at: 2932 2932
Size: 6004
SacII CC_GC'GG
Cuts at: 2182 2182
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SexAI A'CCwGG_T
Cuts at: 2019 2019
Size: 6004
SfcI C'TryA_G
Cuts at: 826 1045 2888 3281 3352 4487 5168 5359 826
Size: 219 1843 393 71 1135 681 191 1471
Fragments arranged by size:
1843 1471 1135 681 393 219 191 71
SfoI GGC'GCC
Cuts at: 2867 2867
Size: 6004
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 2159 152
Size: 2007 3997
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 2249 2402 2861 3212 1517
Size: 732 153 459 351 4309
Fragments arranged by size:
4309 732 459 351 153
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 2595 3104 3443 513
Size: 552 939 57 534 509 339 3074
Fragments arranged by size:
3074 939 552 534 509 339 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2255 4250 5962
Size: 274 80 33 51 1443 278 1995 1712
Cuts at: 5962 96
Size: 138
Fragments arranged by size:
1995 1712 1443 278 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3127 3159 5964 5996 3127
Size: 32 2805 32 3135
Fragments arranged by size:
3135 2805 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2587 2431
Size: 156 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 4133 4133
Size: 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SanDI SapI SfiI SrfI SwaI