Restriction map for
pFN21AE3941
(Circular) (Six-base) MAPSORT of: pFN21AE3941.seq Check: 4144 from: 1 to: 6466
pFN21AE3941 6466 bp
With 182 enzymes: SgfI *
March 28, 2012 11:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3066 3809 1937
Size: 1129 743 4594
Fragments arranged by size:
4594 1129 743
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 4593 4966 1563
Size: 3030 373 3063
Fragments arranged by size:
3063 3030 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4529 5913 6181 1632
Size: 2897 1384 268 1917
Fragments arranged by size:
2897 1917 1384 268
AfeI AGC'GCT
Cuts at: 3353 3353
Size: 6466
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6269
AflIII A'CryG_T
Cuts at: 1203 3321 5365 6342 1203
Size: 2118 2044 977 1327
Fragments arranged by size:
2118 2044 1327 977
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 2837 3102 3521 1166
Size: 1671 265 419 4111
Fragments arranged by size:
4111 1671 419 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2465 1184
Size: 1281 5185
AlwNI CAG_nnn'CTG
Cuts at: 2017 2077 2248 3059 4209 5781 2017
Size: 60 171 811 1150 1572 2702
Fragments arranged by size:
2702 1572 1150 811 171 60
ApaI G_GGCC'C
Cuts at: 2595 2595
Size: 6466
ApaLI G'TGCA_C
Cuts at: 3325 4523 5679 3325
Size: 1198 1156 4112
Fragments arranged by size:
4112 1198 1156
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2595 3029 3446 3638 3791 1870
Size: 73 574 78 434 417 192 153 4545
Fragments arranged by size:
4545 574 434 417 192 153 78 73
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 2497 2680 2753 3746
Size: 325 217 627 564 146 183 73 993
Cuts at: 3746 3787 5242 618
Size: 41 1455 1842
Fragments arranged by size:
1842 1455 993 627 564 325 217 183
146 73 41
BanII G_rGCy'C
Cuts at: 1391 2378 2430 2438 2595 3419 3668 3785 1391
Size: 987 52 8 157 824 249 117 4072
Fragments arranged by size:
4072 987 824 249 157 117 52 8
BbeI G_GCGC'C
Cuts at: 2757 2757
Size: 6466
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4657 4691 1903
Size: 34 2720 34 3678
Fragments arranged by size:
3678 2720 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2984 4358 5568 2984
Size: 1374 1210 3882
Fragments arranged by size:
3882 1374 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6466
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2082 2129 2138
Size: 107 122 71 631 605 410 47 9
Cuts at: 2138 2471 2621 5077 136
Size: 333 150 2456 1525
Fragments arranged by size:
2456 1525 631 605 410 333 150 122
107 71 47 9
BglII A'GATC_T
Cuts at: 6461 6461
Size: 6466
BlpI GC'TnA_GC
Cuts at: 3883 3883
Size: 6466
Bme1580I G_kGCm'C
Cuts at: 1790 2502 2595 2642 2685 3329 3438 3749 4527
Size: 712 93 47 43 644 109 311 778
Cuts at: 4527 5683 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 778 712 644 311 109 93
47 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4197 5155 442
Size: 416 53 83 399 24 2780 958 1753
Fragments arranged by size:
2780 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 6466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2603 2939 3062 5126 1239
Size: 1364 336 123 2064 2579
Fragments arranged by size:
2579 2064 1364 336 123
Bpu10I CC'TnA_GC
Cuts at: 2904 2904
Size: 6466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4591 5456 5754 4591
Size: 865 298 5303
Fragments arranged by size:
5303 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5129 882
Size: 617 3630 2219
Fragments arranged by size:
3630 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4257 493
Size: 711 3053 2702
Fragments arranged by size:
3053 2702 711
BsaBI GATnn'nnATC
Cuts at: 2864 6460 2864
Size: 3596 2870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 2754 3510 3540
Size: 53 83 186 936 882 339 756 30
Cuts at: 3540 3585 3716 4273 4655 5290 275
Size: 45 131 557 382 635 1451
Fragments arranged by size:
1451 936 882 756 635 557 382 339
186 131 83 53 45 30
BsaWI w'CCGG_w
Cuts at: 4899 5297 5571 5718 6129 4899
Size: 398 274 147 411 5236
Fragments arranged by size:
5236 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2405 2435 2834 2864 1178
Size: 30 165 30 1002 30 399 30 4780
Fragments arranged by size:
4780 1002 399 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2816 2825 2858 3608 1186
Size: 1630 9 33 750 4044
Fragments arranged by size:
4044 1630 750 33 9
BseYI C'CCAG_C
Cuts at: 2053 2080 2684 3215 3281 3701 5669 6165 2053
Size: 27 604 531 66 420 1968 496 2354
Fragments arranged by size:
2354 1968 604 531 496 420 66 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2003 3126 1163
Size: 573 267 1123 4503
Fragments arranged by size:
4503 1123 573 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2316 2460 2553 3726 3916 4677
Size: 275 186 315 144 93 1173 190 761
Cuts at: 4677 5705 1540
Size: 1028 2301
Fragments arranged by size:
2301 1173 1028 761 315 275 190 186
144 93
BsiHKAI G_wGCw'C
Cuts at: 2378 3329 4527 4612 5683 2378
Size: 951 1198 85 1071 3161
Fragments arranged by size:
3161 1198 1071 951 85
BsmI GAATG_Cn'
Cuts at: 3977 4070 3977
Size: 93 6373
BsmBI CGTCTCn'nnnn_
Cuts at: 2579 2579
Size: 6466
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2378 2430 2438 2502 2595 2642 2685
Size: 399 588 52 8 64 93 47 43
Cuts at: 2685 3329 3419 3438 3668 3749 3785 4527 4612
Size: 644 90 19 230 81 36 742 85
Cuts at: 4612 5683 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 644 588 399 230 93
90 85 81 64 52 47 43 36
19 8
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 4355 6085 4355
Size: 1730 4736
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2974 3821 844
Size: 2130 847 3489
Fragments arranged by size:
3489 2130 847
BsrBI CCG'CTC
Cuts at: 1394 1823 2740 2928 3198 3499 4353 1394
Size: 429 917 188 270 301 854 3507
Fragments arranged by size:
3507 917 854 429 301 270 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 4961 5135 65
Size: 1183 3713 174 1396
Fragments arranged by size:
3713 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 2417 2455 2548 2552 3587 3718
Size: 110 505 102 38 93 4 1035 131
Cuts at: 3718 5110 5297 1700
Size: 1392 187 2869
Fragments arranged by size:
2869 1392 1035 505 187 131 110 102
93 38 4
BsrGI T'GTAC_A
Cuts at: 96 2307 2862 96
Size: 2211 555 3700
Fragments arranged by size:
3700 2211 555
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 2297 2954 4526 5538 2297
Size: 657 1572 1012 3225
Fragments arranged by size:
3225 1572 1012 657
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 794
Size: 1283 5183
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6466
BstXI CCAn_nnnn'nTGG
Cuts at: 3523 3523
Size: 6466
BstYI r'GATC_y
Cuts at: 1972 2028 2244 2328 3796 4163 4548 4565 6017
Size: 56 216 84 1468 367 385 17 1452
Cuts at: 6017 6103 6115 6461 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1468 1452 385 367 346 216 86
84 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2469 2535 2616 2835 3315 3462 3726
Size: 552 1404 66 81 219 480 147 264
Cuts at: 3726 513
Size: 3253
Fragments arranged by size:
3253 1404 552 480 264 219 147 81
66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2109 487
Size: 683 56 883 4844
Fragments arranged by size:
4844 883 683 56
BtsI GCAGTG_nn'
Cuts at: 2383 2895 3982 4776 4796 2383
Size: 512 1087 794 20 4053
Fragments arranged by size:
4053 1087 794 512 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3136 3171 529
Size: 35 2572 35 3824
Fragments arranged by size:
3824 2572 35 35
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DraIII CAC_nnn'GTG
Cuts at: 2341 2341
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3239 5473 809
Size: 507 1923 2234 1802
Fragments arranged by size:
2234 1923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2283 2313
Size: 54 1425 50 275 18 318 135 30
Cuts at: 2313 2457 2550 2613 2691 2854 3079 3144 3171
Size: 144 93 63 78 163 225 65 27
Cuts at: 3171 3630 3658 3723 3913 4802 6431 8
Size: 459 28 65 190 889 1629 43
Fragments arranged by size:
1629 1425 889 459 318 275 225 190
163 144 135 93 78 65 65 63
54 50 43 30 28 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2313 2457 2550 3723 3913 1537
Size: 275 501 144 93 1173 190 4090
Fragments arranged by size:
4090 1173 501 275 190 144 93
EarI CTCTTCn'nnn_
Cuts at: 1353 4396 1353
Size: 3043 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2933 3409 5053 5427 5573 387
Size: 1064 138 1344 476 1644 374 146 1280
Fragments arranged by size:
1644 1344 1280 1064 476 374 146 138
EcoICRI GAG'CTC
Cuts at: 2376 2376
Size: 6466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 2939 3062 4529 5126 5913 6181
Size: 393 971 336 123 1467 597 787 268
Cuts at: 6181 1239
Size: 1524
Fragments arranged by size:
1524 1467 971 787 597 393 336 268
123
EcoO109I rG'GnC_Cy
Cuts at: 2591 2592 3025 3241 3708 3759 2591
Size: 1 433 216 467 51 5298
Fragments arranged by size:
5298 467 433 216 51 1
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 6466
FseI GG_CCGG'CC
Cuts at: 2556 2556
Size: 6466
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 2077 2202 2578 2757 3355 5613 1678
Size: 399 125 376 179 598 2258 2531
Fragments arranged by size:
2531 2258 598 399 376 179 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2405 2437 5107 5139 5181
Size: 32 135 32 833 32 2670 32 42
Cuts at: 5181 5213 1373
Size: 32 2626
Fragments arranged by size:
2670 2626 833 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3553 3810 4056 669
Size: 988 281 1615 257 246 3079
Fragments arranged by size:
3079 1615 988 281 257 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KasI G'GCGC_C
Cuts at: 2753 2753
Size: 6466
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5580 5764 1305
Size: 4275 184 2007
Fragments arranged by size:
4275 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2693 3146 3173 3660 10
Size: 54 1425 343 861 453 27 487 2816
Fragments arranged by size:
2816 1425 861 487 453 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 3102 3410 3521 4424 4783 4942
Size: 648 1671 265 308 111 903 359 159
Cuts at: 4942 518
Size: 2042
Fragments arranged by size:
2042 1671 903 648 359 308 265 159
111
MspA1I CmG'CkG
Cuts at: 1606 2184 2402 2449 2471 2509 2581 2618 2917
Size: 578 218 47 22 38 72 37 299
Cuts at: 2917 2997 3085 3180 3229 3317 3464 3646 3728
Size: 80 88 95 49 88 147 182 82
Cuts at: 3728 3882 4559 5707 5952 1606
Size: 154 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 578 299 245 218 182
154 147 95 88 88 82 80 72
49 47 38 37 22
NaeI GCC'GGC
Cuts at: 2317 2419 2457 2550 2554 3589 3720 2317
Size: 102 38 93 4 1035 131 5063
Fragments arranged by size:
5063 1035 131 102 93 38 4
NarI GG'CG_CC
Cuts at: 2754 2754
Size: 6466
NcoI C'CATG_G
Cuts at: 513 1065 2535 2835 513
Size: 552 1470 300 4144
Fragments arranged by size:
4144 1470 552 300
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NgoMIV G'CCGG_C
Cuts at: 2315 2417 2455 2548 2552 3587 3718 2315
Size: 102 38 93 4 1035 131 5063
Fragments arranged by size:
5063 1035 131 102 93 38 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3723 3913 3723
Size: 190 6276
NspI r_CATG'y
Cuts at: 1132 1229 3325 3824 5369 1132
Size: 97 2096 499 1545 2229
Fragments arranged by size:
2229 2096 1545 499 97
PasI CC'CwG_GG
Cuts at: 1348 2731 1348
Size: 1383 5083
PciI A'CATG_T
Cuts at: 3321 5365 3321
Size: 2044 4422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3628 1108
Size: 2520 3946
PfoI T'CCnGG_A
Cuts at: 3621 3621
Size: 6466
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3025 3241 3759 3025
Size: 216 518 5732
Fragments arranged by size:
5732 518 216
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PspOMI G'GGCC_C
Cuts at: 2591 2591
Size: 6466
PstI C_TGCA'G
Cuts at: 830 2328 2454 3818 830
Size: 1498 126 1364 3478
Fragments arranged by size:
3478 1498 1364 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
PvuII CAG'CTG
Cuts at: 2449 3085 3180 2449
Size: 636 95 5735
Fragments arranged by size:
5735 636 95
SacI G_AGCT'C
Cuts at: 2378 2378
Size: 6466
SacII CC_GC'GG
Cuts at: 2472 2619 3318 3465 3729 2472
Size: 147 699 147 264 5209
Fragments arranged by size:
5209 699 264 147 147
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SanDI GG'GwC_CC
Cuts at: 3025 3025
Size: 6466
SbfI CC_TGCA'GG
Cuts at: 3818 3818
Size: 6466
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SfcI C'TryA_G
Cuts at: 826 1045 2324 2450 3814 4949 5630 5821 826
Size: 219 1279 126 1364 1135 681 191 1471
Fragments arranged by size:
1471 1364 1279 1135 681 219 191 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 2129 2129
Size: 6466
SfoI GGC'GCC
Cuts at: 2755 2755
Size: 6466
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 3587 3718 1700
Size: 1887 131 4448
Fragments arranged by size:
4448 1887 131
SmaI CCC'GGG
Cuts at: 1872 2597 3031 3793 1872
Size: 725 434 762 4545
Fragments arranged by size:
4545 762 725 434
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4570 5471 5733 820
Size: 197 926 2627 901 262 1553
Fragments arranged by size:
2627 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 2123 2562 2825 2873 1517
Size: 606 439 263 48 5110
Fragments arranged by size:
5110 606 439 263 48
StyI C'CwwG_G
Cuts at: 513 1065 2535 2835 3762 3905 513
Size: 552 1470 300 927 143 3074
Fragments arranged by size:
3074 1470 927 552 300 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3604 4493 4510 4663 4848 5293 325
Size: 1255 2024 889 17 153 185 445 1498
Fragments arranged by size:
2024 1498 1255 889 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 2862 3261
Size: 274 80 33 51 1443 330 555 399
Cuts at: 3261 4712 6424 96
Size: 1451 1712 138
Fragments arranged by size:
1712 1451 1443 555 399 330 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6426 6458 6426
Size: 32 6434
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6466
XbaI T'CTAG_A
Cuts at: 3802 3802
Size: 6466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 2578
Size: 6466
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6466
XmaI C'CCGG_G
Cuts at: 1870 2595 3029 3791 1870
Size: 725 434 762 4545
Fragments arranged by size:
4545 762 725 434
XmnI GAAnn'nnTTC
Cuts at: 4595 4595
Size: 6466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I EcoNI EcoRV FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SexAI SrfI SwaI