Restriction map for
pFN21AE3933
(Circular) (Six-base) MAPSORT of: pFN21AE3933.seq Check: 7694 from: 1 to: 5368
pFN21AE3933 5368 bp
With 182 enzymes: SgfI *
March 28, 2012 11:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3178 278
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2711 1937
Size: 774 4594
Acc65I G'GTAC_C
Cuts at: 2689 2689
Size: 5368
AclI AA'CG_TT
Cuts at: 1563 3495 3868 1563
Size: 1932 373 3063
Fragments arranged by size:
3063 1932 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2305 3431 4815 5083 1632
Size: 673 1126 1384 268 1917
Fragments arranged by size:
1917 1384 1126 673 268
AfeI AGC'GCT
Cuts at: 2058 2058
Size: 5368
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5171
AflIII A'CryG_T
Cuts at: 1203 4267 5244 1203
Size: 3064 977 1327
Fragments arranged by size:
3064 1327 977
AgeI A'CCGG_T
Cuts at: 4199 4199
Size: 5368
AhdI GACnn_n'nnGTC
Cuts at: 4097 4097
Size: 5368
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2633 1184
Size: 1449 3919
AlwNI CAG_nnn'CTG
Cuts at: 2148 2384 3111 4683 2148
Size: 236 727 1572 2833
Fragments arranged by size:
2833 1572 727 236
ApaLI G'TGCA_C
Cuts at: 3425 4581 3425
Size: 1156 4212
ApoI r'AATT_y
Cuts at: 1493 2677 2903 3055 5164 1493
Size: 1184 226 152 2109 1697
Fragments arranged by size:
2109 1697 1184 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3922 160
Size: 872 2890 1606
Fragments arranged by size:
2890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
AvaI C'yCGr_G
Cuts at: 1870 1943 2182 2341 2373 2693 1870
Size: 73 239 159 32 320 4545
Fragments arranged by size:
4545 320 239 159 73 32
BamHI G'GATC_C
Cuts at: 2698 3065 2698
Size: 367 5001
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 2632 2689 4144 618
Size: 325 217 627 255 590 57 1455 1842
Fragments arranged by size:
1842 1455 627 590 325 255 217 57
BanII G_rGCy'C
Cuts at: 1391 2109 2256 2532 2687 1391
Size: 718 147 276 155 4072
Fragments arranged by size:
4072 718 276 155 147
BbeI G_GCGC'C
Cuts at: 2046 2046
Size: 5368
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4397
BbvCI CC'TCA_GC
Cuts at: 2546 2546
Size: 5368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3559 3593 1903
Size: 34 1622 34 3678
Fragments arranged by size:
3678 1622 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3260 4470 3260
Size: 1210 4158
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3979 136
Size: 107 122 71 631 605 337 1970 1525
Fragments arranged by size:
1970 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5363 5363
Size: 5368
BlpI GC'TnA_GC
Cuts at: 2785 2785
Size: 5368
Bme1580I G_kGCm'C
Cuts at: 1790 3429 4585 1790
Size: 1639 1156 2573
Fragments arranged by size:
2573 1639 1156
BmgBI CAC'GTC
Cuts at: 1318 2088 1318
Size: 770 4598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2259 3099 4057
Size: 416 53 83 399 24 842 840 958
Cuts at: 4057 442
Size: 1753
Fragments arranged by size:
1753 958 842 840 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 4028 1239
Size: 1349 1440 2579
Fragments arranged by size:
2579 1440 1349
Bpu10I CC'TnA_GC
Cuts at: 2546 2546
Size: 5368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3493 4358 4656 3493
Size: 865 298 4205
Fragments arranged by size:
4205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2361 4031 882
Size: 617 862 1670 2219
Fragments arranged by size:
2219 1670 862 617
BsaAI yAC'GTr
Cuts at: 493 1204 3159 493
Size: 711 1955 2702
Fragments arranged by size:
2702 1955 711
BsaBI GATnn'nnATC
Cuts at: 5362 5362
Size: 5368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 3175 3557 4192
Size: 53 83 186 936 510 1132 382 635
Cuts at: 4192 275
Size: 1451
Fragments arranged by size:
1451 1132 936 635 510 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2586 3801 4199 4473 4620 5031 2586
Size: 1215 398 274 147 411 2923
Fragments arranged by size:
2923 1215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5143
Fragments arranged by size:
5143 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5368
BseYI C'CCAG_C
Cuts at: 2172 4571 5067 2172
Size: 2399 496 2473
Fragments arranged by size:
2473 2399 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 1163
Size: 573 798 3997
Fragments arranged by size:
3997 798 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2367 2464 2818 3579 4607 1540
Size: 275 186 366 97 354 761 1028 2301
Fragments arranged by size:
2301 1028 761 366 354 275 186 97
BsiHKAI G_wGCw'C
Cuts at: 2093 2109 2352 2478 2487 2532 3429 3514 4585
Size: 16 243 126 9 45 897 85 1071
Cuts at: 4585 2093
Size: 2876
Fragments arranged by size:
2876 1071 897 243 126 85 45 16
9
BsmI GAATG_Cn'
Cuts at: 2879 2972 2879
Size: 93 5275
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2109 2256 2352 2478 2487 2532
Size: 399 303 16 147 96 126 9 45
Cuts at: 2532 2687 3429 3514 4585 1391
Size: 155 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 155 147 126
96 85 45 16 9
BspEI T'CCGG_A
Cuts at: 5031 5031
Size: 5368
BspHI T'CATG_A
Cuts at: 3257 4987 3257
Size: 1730 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2723 844
Size: 1879 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3255 1394
Size: 429 1432 3507
Fragments arranged by size:
3507 1432 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3863 4037 65
Size: 1183 2615 174 1396
Fragments arranged by size:
2615 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 2039 2119 4012 4199 1700
Size: 110 217 12 80 1893 187 2869
Fragments arranged by size:
2869 1893 217 187 110 80 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5368
BssHII G'CGCG_C
Cuts at: 1925 2214 2258 1925
Size: 289 44 5035
Fragments arranged by size:
5035 289 44
BssSI C'ACGA_G
Cuts at: 3428 4440 3428
Size: 1012 4356
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2384 2491 794
Size: 1590 107 3671
Fragments arranged by size:
3671 1590 107
BstBI TT'CG_AA
Cuts at: 4172 4172
Size: 5368
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5368
BstXI CCAn_nnnn'nTGG
Cuts at: 2452 2452
Size: 5368
BstYI r'GATC_y
Cuts at: 1972 2698 3065 3450 3467 4919 5005 5017 5363
Size: 726 367 385 17 1452 86 12 346
Cuts at: 5363 1972
Size: 1977
Fragments arranged by size:
1977 1452 726 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2021 2331 2367 2592 2648 513
Size: 552 939 17 310 36 225 56 3233
Fragments arranged by size:
3233 939 552 310 225 56 36 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4629
Fragments arranged by size:
4629 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 2249 2884 3678 3698 2228
Size: 21 635 794 20 3898
Fragments arranged by size:
3898 794 635 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5368
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5333
DraI TTT'AAA
Cuts at: 2672 2672
Size: 5368
DraIII CAC_nnn'GTG
Cuts at: 2491 2491
Size: 5368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4375 809
Size: 507 3059 1802
Fragments arranged by size:
3059 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2301 2364
Size: 54 1425 50 275 18 307 164 63
Cuts at: 2364 2815 3704 5333 8
Size: 451 889 1629 43
Fragments arranged by size:
1629 1425 889 451 307 275 164 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2364 2815 1537
Size: 275 552 451 4090
Fragments arranged by size:
4090 552 451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3298 1353
Size: 1945 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3955 4329 4475 387
Size: 1064 138 2366 374 146 1280
Fragments arranged by size:
2366 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2107 2530 2107
Size: 423 4945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2588 3431 4028 4815 5083 1239
Size: 393 673 283 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 673 597 393 283 268
EcoO109I rG'GnC_Cy
Cuts at: 2642 2642
Size: 5368
EcoRI G'AATT_C
Cuts at: 2677 3055 2677
Size: 378 4990
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 5368
FspI TGC'GCA
Cuts at: 3874 3874
Size: 5368
HaeII r_GCGC'y
Cuts at: 1678 2046 2060 2170 2182 2320 4515 1678
Size: 368 14 110 12 138 2195 2531
Fragments arranged by size:
2531 2195 368 138 110 14 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2339 2371 2430
Size: 32 135 32 536 32 199 32 59
Cuts at: 2430 2462 4009 4041 4083 4115 1373
Size: 32 1547 32 42 32 2626
Fragments arranged by size:
2626 1547 536 199 135 59 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2712 2958 669
Size: 988 281 774 246 3079
Fragments arranged by size:
3079 988 774 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5368
HpaI GTT'AAC
Cuts at: 2958 2958
Size: 5368
KasI G'GCGC_C
Cuts at: 2042 2042
Size: 5368
KpnI G_GTAC'C
Cuts at: 2693 2693
Size: 5368
MfeI C'AATT_G
Cuts at: 2967 2967
Size: 5368
MluI A'CGCG_T
Cuts at: 5244 5244
Size: 5368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4482 4666 1305
Size: 3177 184 2007
Fragments arranged by size:
3177 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 10
Size: 54 1425 343 471 3075
Fragments arranged by size:
3075 1425 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2450 3326 3685 3844 518
Size: 648 1284 876 359 159 2042
Fragments arranged by size:
2042 1284 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2272 2333 2369 2381 2391 2500 2784
Size: 417 249 61 36 12 10 109 284
Cuts at: 2784 3461 4609 4854 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 417 284 249 245 109
61 36 12 10
NarI GG'CG_CC
Cuts at: 2043 2043
Size: 5368
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3877
Fragments arranged by size:
3877 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5368
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5368
NotI GC'GGCC_GC
Cuts at: 2364 2815 2364
Size: 451 4917
NspI r_CATG'y
Cuts at: 1132 1229 2726 4271 1132
Size: 97 1497 1545 2229
Fragments arranged by size:
2229 1545 1497 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5368
PciI A'CATG_T
Cuts at: 4267 4267
Size: 5368
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5368
PmeI GTTT'AAAC
Cuts at: 2672 2672
Size: 5368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 4950 4982 3437
Size: 32 1481 32 3823
Fragments arranged by size:
3823 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5368
PsiI TTA'TAA
Cuts at: 2938 2938
Size: 5368
PstI C_TGCA'G
Cuts at: 830 2076 2720 830
Size: 1246 644 3478
Fragments arranged by size:
3478 1246 644
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5368
PvuII CAG'CTG
Cuts at: 2272 2272
Size: 5368
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 5368
SacI G_AGCT'C
Cuts at: 2109 2532 2109
Size: 423 4945
SacII CC_GC'GG
Cuts at: 2024 2334 2370 2024
Size: 310 36 5022
Fragments arranged by size:
5022 310 36
SalI G'TCGA_C
Cuts at: 1936 2710 1936
Size: 774 4594
SbfI CC_TGCA'GG
Cuts at: 2720 2720
Size: 5368
ScaI AGT'ACT
Cuts at: 3616 5328 3616
Size: 1712 3656
SfcI C'TryA_G
Cuts at: 826 1045 2072 2716 3851 4532 4723 826
Size: 219 1027 644 1135 681 191 1471
Fragments arranged by size:
1471 1135 1027 681 644 219 191
SfoI GGC'GCC
Cuts at: 2044 2044
Size: 5368
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5368
SmaI CCC'GGG
Cuts at: 1872 2184 2343 2375 2695 1872
Size: 312 159 32 320 4545
Fragments arranged by size:
4545 320 312 159 32
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3472 4373 4635 820
Size: 197 926 1529 901 262 1553
Fragments arranged by size:
1553 1529 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3159 493
Size: 2666 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5368
SphI G_CATG'C
Cuts at: 1132 2726 1132
Size: 1594 3774
SrfI GCCC'GGGC
Cuts at: 2184 2375 2184
Size: 191 5177
SspI AAT'ATT
Cuts at: 5 52 3292 5
Size: 47 3240 2081
Fragments arranged by size:
3240 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5368
StyI C'CwwG_G
Cuts at: 513 1065 2004 2523 2807 513
Size: 552 939 519 284 3074
Fragments arranged by size:
3074 939 552 519 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3395 3412 3565 3750 4195 325
Size: 1255 1815 17 153 185 445 1498
Fragments arranged by size:
1815 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3614 5326 96
Size: 274 80 33 51 1443 1637 1712 138
Fragments arranged by size:
1712 1637 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5328 5360 5328
Size: 32 5336
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5368
XbaI T'CTAG_A
Cuts at: 2704 2704
Size: 5368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2287 2530 2011
Size: 276 243 4849
Fragments arranged by size:
4849 276 243
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5368
XmaI C'CCGG_G
Cuts at: 1870 2182 2341 2373 2693 1870
Size: 312 159 32 320 4545
Fragments arranged by size:
4545 320 312 159 32
XmnI GAAnn'nnTTC
Cuts at: 3497 3497
Size: 5368
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3176 276
Size: 53 83 186 936 1642 2468
Fragments arranged by size:
2468 1642 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI SanDI SapI SexAI SfiI SwaI