Restriction map for
pFN21AE3930
(Circular) (Six-base) MAPSORT of: pFN21AE3930.seq Check: 1256 from: 1 to: 5908
pFN21AE3930 5908 bp
With 182 enzymes: SgfI *
March 28, 2012 11:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2227 2227
Size: 5908
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3718 278
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3251 1937
Size: 1314 4594
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2716 2744 3971 5355 5623 1632
Size: 1084 28 1227 1384 268 1917
Fragments arranged by size:
1917 1384 1227 1084 268 28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2135 2324 2740 1184
Size: 951 189 416 4352
Fragments arranged by size:
4352 951 416 189
AlwNI CAG_nnn'CTG
Cuts at: 2128 2266 3143 3651 5223 2128
Size: 138 877 508 1572 2813
Fragments arranged by size:
2813 1572 877 508 138
ApaI G_GGCC'C
Cuts at: 2107 2220 2107
Size: 113 5795
ApaLI G'TGCA_C
Cuts at: 3965 5121 3965
Size: 1156 4752
ApoI r'AATT_y
Cuts at: 1493 3217 3443 3595 5704 1493
Size: 1724 226 152 2109 1697
Fragments arranged by size:
2109 1724 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2132 2132
Size: 5908
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2049 2606 2699 2864 3233 1870
Size: 73 84 22 557 93 165 369 4545
Fragments arranged by size:
4545 557 369 165 93 84 73 22
BamHI G'GATC_C
Cuts at: 2970 3238 3605 2970
Size: 268 367 5273
Fragments arranged by size:
5273 367 268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2032 2167 2610 2654
Size: 325 217 627 236 9 135 443 44
Cuts at: 2654 2703 3229 4684 618
Size: 49 526 1455 1842
Fragments arranged by size:
1842 1455 627 526 443 325 236 217
135 49 44 9
BanII G_rGCy'C
Cuts at: 1391 2013 2107 2196 2220 2631 3227 1391
Size: 622 94 89 24 411 596 4072
Fragments arranged by size:
4072 622 596 411 94 89 24
BbeI G_GCGC'C
Cuts at: 2027 2658 2707 2027
Size: 631 49 5228
Fragments arranged by size:
5228 631 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4937
BbvCI CC'TCA_GC
Cuts at: 3190 3190
Size: 5908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4099 4133 1903
Size: 34 2162 34 3678
Fragments arranged by size:
3678 2162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3203 4519 136
Size: 107 122 71 631 605 1531 1316 1525
Fragments arranged by size:
1531 1525 1316 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 2472 3325 2472
Size: 853 5055
Bme1580I G_kGCm'C
Cuts at: 1790 2107 2114 2220 2613 3969 5125 1790
Size: 317 7 106 393 1356 1156 2573
Fragments arranged by size:
2573 1356 1156 393 317 106 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5908
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3639 4597 442
Size: 416 53 83 399 24 2222 958 1753
Fragments arranged by size:
2222 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 3120 4568 1239
Size: 971 910 1448 2579
Fragments arranged by size:
2579 1448 971 910
Bpu10I CC'TnA_GC
Cuts at: 3190 3190
Size: 5908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2783 4033 4898 5196 2336
Size: 447 1250 865 298 3048
Fragments arranged by size:
3048 1250 865 447 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2052 2417 2988 3071 4571 882
Size: 617 553 365 571 83 1500 2219
Fragments arranged by size:
2219 1500 617 571 553 365 83
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 2655 2704 3715
Size: 53 83 186 936 491 631 49 1011
Cuts at: 3715 4097 4732 275
Size: 382 635 1451
Fragments arranged by size:
1451 1011 936 635 631 491 382 186
83 53 49
BsaWI w'CCGG_w
Cuts at: 2886 4341 4739 5013 5160 5571 2886
Size: 1455 398 274 147 411 3223
Fragments arranged by size:
3223 1455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 1178
Size: 30 165 30 669 30 4984
Fragments arranged by size:
4984 669 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 2342 2756 3131 1186
Size: 1111 45 414 375 3963
Fragments arranged by size:
3963 1111 414 375 45
BseYI C'CCAG_C
Cuts at: 2040 2505 3095 5111 5607 2040
Size: 465 590 2016 496 2341
Fragments arranged by size:
2341 2016 590 496 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3075 1163
Size: 573 1339 3996
Fragments arranged by size:
3996 1339 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 2160 2643 3358 4119 5147
Size: 275 186 75 84 483 715 761 1028
Cuts at: 5147 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 715 483 275 186 84
75
BsiHKAI G_wGCw'C
Cuts at: 2196 3969 4054 5125 2196
Size: 1773 85 1071 2979
Fragments arranged by size:
2979 1773 1071 85
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2107 2114 2196 2220 2613 2631
Size: 399 223 94 7 82 24 393 18
Cuts at: 2631 3227 3969 4054 5125 1391
Size: 596 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 596 399 393 223 94
85 82 24 18 7
BspEI T'CCGG_A
Cuts at: 2886 5571 2886
Size: 2685 3223
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2227 2412 3263 844
Size: 1383 185 851 3489
Fragments arranged by size:
3489 1383 851 185
BsrBI CCG'CTC
Cuts at: 1394 1823 2767 3795 1394
Size: 429 944 1028 3507
Fragments arranged by size:
3507 1028 944 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2251 2645 4552 4739 1700
Size: 110 441 394 1907 187 2869
Fragments arranged by size:
2869 1907 441 394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 2132 2311 2332 2334 2512 1925
Size: 207 179 21 2 178 5321
Fragments arranged by size:
5321 207 179 178 21 2
BssSI C'ACGA_G
Cuts at: 3968 4980 3968
Size: 1012 4896
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 794
Size: 1472 4436
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstXI CCAn_nnnn'nTGG
Cuts at: 2549 2878 2549
Size: 329 5579
BstYI r'GATC_y
Cuts at: 1972 2125 2754 2970 3238 3605 3990 4007 5459
Size: 153 629 216 268 367 385 17 1452
Cuts at: 5459 5545 5557 5903 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 629 385 367 346 268 216
153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2670 2670
Size: 5908
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2076 2211 2966 513
Size: 552 939 54 18 135 755 3455
Fragments arranged by size:
3455 939 755 552 135 54 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5169
Fragments arranged by size:
5169 683 56
BtsI GCAGTG_nn'
Cuts at: 2729 2953 3424 4218 4238 2729
Size: 224 471 794 20 4399
Fragments arranged by size:
4399 794 471 224 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2846 4915 809
Size: 507 1530 2069 1802
Fragments arranged by size:
2069 1802 1530 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2157 2416
Size: 54 1425 50 275 18 243 84 259
Cuts at: 2416 2493 3355 4244 5873 8
Size: 77 862 889 1629 43
Fragments arranged by size:
1629 1425 889 862 275 259 243 84
77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2073 2157 3355 1537
Size: 275 261 84 1198 4090
Fragments arranged by size:
4090 1198 275 261 84
EarI CTCTTCn'nnn_
Cuts at: 1353 3108 3838 1353
Size: 1755 730 3423
Fragments arranged by size:
3423 1755 730
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 2696 3138 4495 4869 5015
Size: 1064 138 681 426 442 1357 374 146
Cuts at: 5015 387
Size: 1280
Fragments arranged by size:
1357 1280 1064 681 442 426 374 146
138
EcoICRI GAG'CTC
Cuts at: 2194 2194
Size: 5908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2716 2744 3120 3971 4568 5355
Size: 393 578 506 28 376 851 597 787
Cuts at: 5355 5623 1239
Size: 268 1524
Fragments arranged by size:
1524 851 787 597 578 506 393 376
268 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2798 2798
Size: 5908
EcoO109I rG'GnC_Cy
Cuts at: 2103 2104 2216 2674 2848 3186 2103
Size: 1 112 458 174 338 4825
Fragments arranged by size:
4825 458 338 174 112 1
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 4414 4414
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2027 2189 2296 2455 2542 2658 2707 5055
Size: 349 162 107 159 87 116 49 2348
Cuts at: 5055 1678
Size: 2531
Fragments arranged by size:
2531 2348 349 162 159 116 107 87
49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4549 4581 4623 4655 1373
Size: 32 135 32 2977 32 42 32 2626
Fragments arranged by size:
2977 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3252 3498 669
Size: 988 281 1314 246 3079
Fragments arranged by size:
3079 1314 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2023 2654 2703 2023
Size: 631 49 5228
Fragments arranged by size:
5228 631 49
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5022 5206 1305
Size: 3717 184 2007
Fragments arranged by size:
3717 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3866 4225 4384 518
Size: 648 1710 990 359 159 2042
Fragments arranged by size:
2042 1710 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2078 2374 2446 2509 2824 2896 3324
Size: 454 18 296 72 63 315 72 428
Cuts at: 3324 4001 5149 5394 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 454 428 315 296 245
72 72 63 18
NaeI GCC'GGC
Cuts at: 2253 2647 2253
Size: 394 5514
NarI GG'CG_CC
Cuts at: 2024 2655 2704 2024
Size: 631 49 5228
Fragments arranged by size:
5228 631 49
NcoI C'CATG_G
Cuts at: 513 1065 2004 2966 513
Size: 552 939 962 3455
Fragments arranged by size:
3455 962 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NgoMIV G'CCGG_C
Cuts at: 2251 2645 2251
Size: 394 5514
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 3266 4811 1132
Size: 97 2037 1545 2229
Fragments arranged by size:
2229 2037 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2107 2660 1348
Size: 759 553 4596
Fragments arranged by size:
4596 759 553
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5908
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 5490 5522 3977
Size: 32 1481 32 4363
Fragments arranged by size:
4363 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2848 2674
Size: 174 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspOMI G'GGCC_C
Cuts at: 2103 2216 2103
Size: 113 5795
PspXI vC'TCGA_Gb
Cuts at: 2864 2864
Size: 5908
PstI C_TGCA'G
Cuts at: 830 2124 2238 2451 3260 830
Size: 1294 114 213 809 3478
Fragments arranged by size:
3478 1294 809 213 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5908
PvuII CAG'CTG
Cuts at: 2446 2509 2446
Size: 63 5845
RsrII CG'GwC_CG
Cuts at: 2365 2365
Size: 5908
SacI G_AGCT'C
Cuts at: 2196 2196
Size: 5908
SacII CC_GC'GG
Cuts at: 2061 2079 2061
Size: 18 5890
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SanDI GG'GwC_CC
Cuts at: 2848 2848
Size: 5908
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 2120 2234 2447 3256 4391 5072 5263
Size: 219 1075 114 213 809 1135 681 191
Cuts at: 5263 826
Size: 1471
Fragments arranged by size:
1471 1135 1075 809 681 219 213 191
114
SfoI GGC'GCC
Cuts at: 2025 2656 2705 2025
Size: 631 49 5228
Fragments arranged by size:
5228 631 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 2645 1700
Size: 945 4963
SmaI CCC'GGG
Cuts at: 1872 2029 2608 3235 1872
Size: 157 579 627 4545
Fragments arranged by size:
4545 627 579 157
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2798 2864 4012 4913 5175
Size: 197 926 408 447 66 1148 901 262
Cuts at: 5175 820
Size: 1553
Fragments arranged by size:
1553 1148 926 901 447 408 262 197
66
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SrfI GCCC'GGGC
Cuts at: 2608 2608
Size: 5908
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5908
StyI C'CwwG_G
Cuts at: 513 1065 2004 2966 3347 513
Size: 552 939 962 381 3074
Fragments arranged by size:
3074 962 939 552 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2382 2441 3109 3935 3952 4105 4290
Size: 1255 802 59 668 826 17 153 185
Cuts at: 4290 4735 325
Size: 445 1498
Fragments arranged by size:
1498 1255 826 802 668 445 185 153
59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4154 5866 96
Size: 274 80 33 51 1443 2177 1712 138
Fragments arranged by size:
2177 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5868 5900 5868
Size: 32 5876
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5908
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XhoI C'TCGA_G
Cuts at: 1943 2864 1943
Size: 921 4987
XmaI C'CCGG_G
Cuts at: 1870 2027 2606 3233 1870
Size: 157 579 627 4545
Fragments arranged by size:
4545 627 579 157
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3716 276
Size: 53 83 186 936 2182 2468
Fragments arranged by size:
2468 2182 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PsrI SapI SexAI SfiI SwaI
XcmI