Restriction map for
pFN21AE3929
(Circular) (Six-base) MAPSORT of: pFN21AE3929.seq Check: 6000 from: 1 to: 6205
pFN21AE3929 6205 bp
With 182 enzymes: SgfI *
March 28, 2012 11:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2455 4268 5652 5920 1632
Size: 823 1813 1384 268 1917
Fragments arranged by size:
1917 1813 1384 823 268
AfeI AGC'GCT
Cuts at: 2025 2025
Size: 6205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2042 1184
Size: 858 5347
AlwNI CAG_nnn'CTG
Cuts at: 2173 2728 2914 3948 5520 2173
Size: 555 186 1034 1572 2858
Fragments arranged by size:
2858 1572 1034 555 186
ApaI G_GGCC'C
Cuts at: 2245 2245
Size: 6205
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 3252 3514 3740 3892 6001 1493
Size: 1759 262 226 152 2109 1697
Fragments arranged by size:
2109 1759 1697 262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2114 2237 3530 1870
Size: 73 171 123 1293 4545
Fragments arranged by size:
4545 1293 171 123 73
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2824 3526 4981 618
Size: 325 217 627 1037 702 1455 1842
Fragments arranged by size:
1842 1455 1037 702 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 2106 2245 2439 2962 3524 1391
Size: 681 34 139 194 523 562 4072
Fragments arranged by size:
4072 681 562 523 194 139 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2071 2526 928
Size: 971 172 455 4607
Fragments arranged by size:
4607 971 455 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2134 2168 4396 4430 1903
Size: 34 50 34 113 34 2228 34 3678
Fragments arranged by size:
3678 2228 113 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4816 136
Size: 107 122 71 631 605 3144 1525
Fragments arranged by size:
3144 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 2438 3105 3622 2438
Size: 667 517 5021
Fragments arranged by size:
5021 667 517
Bme1580I G_kGCm'C
Cuts at: 1790 2245 2259 4266 5422 1790
Size: 455 14 2007 1156 2573
Fragments arranged by size:
2573 2007 1156 455 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2431 3019 3936
Size: 416 53 83 399 24 1014 588 917
Cuts at: 3936 4894 442
Size: 958 1753
Fragments arranged by size:
1753 1014 958 917 588 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2624 2759 2936 4865 1239
Size: 1049 336 135 177 1929 2579
Fragments arranged by size:
2579 1929 1049 336 177 135
Bpu10I CC'TnA_GC
Cuts at: 2954 2954
Size: 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3308 3427 4330 5195 5493 3308
Size: 119 903 865 298 4020
Fragments arranged by size:
4020 903 865 298 119
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 3017 6199 3017
Size: 3182 3023
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2643 4012 4394 5029
Size: 53 83 186 936 1110 1369 382 635
Cuts at: 5029 275
Size: 1451
Fragments arranged by size:
1451 1369 1110 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 2372 2402 2432
Size: 30 165 30 633 30 306 30 30
Cuts at: 2432 2732 2762 1178
Size: 300 30 4621
Fragments arranged by size:
4621 633 306 300 165 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6205
BseYI C'CCAG_C
Cuts at: 2258 2977 5408 5904 2258
Size: 719 2431 496 2559
Fragments arranged by size:
2559 2431 719 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 1163
Size: 573 594 5038
Fragments arranged by size:
5038 594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 2365 3292 3655 4416 5444
Size: 275 186 316 48 927 363 761 1028
Cuts at: 5444 1540
Size: 2301
Fragments arranged by size:
2301 1028 927 761 363 316 275 186
48
BsiHKAI G_wGCw'C
Cuts at: 2160 2439 4266 4351 5422 2160
Size: 279 1827 85 1071 2943
Fragments arranged by size:
2943 1827 1071 279 85
BsmI GAATG_Cn'
Cuts at: 3114 3716 3809 3114
Size: 602 93 5510
Fragments arranged by size:
5510 602 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2106 2160 2245 2259 2439 2962
Size: 399 282 34 54 85 14 180 523
Cuts at: 2962 3524 4266 4351 5422 1391
Size: 562 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 523 399 282 180
85 85 54 34 14
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2727 3560 844
Size: 1883 833 3489
Fragments arranged by size:
3489 1883 833
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4700 4874 65
Size: 1183 3452 174 1396
Fragments arranged by size:
3452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2360 4849 5036 1700
Size: 110 550 2489 187 2869
Fragments arranged by size:
2869 2489 550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 2391 1925
Size: 466 5739
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2293 2266
Size: 27 6178
BstYI r'GATC_y
Cuts at: 1972 2412 2615 3535 3902 4287 4304 5756 5842
Size: 440 203 920 367 385 17 1452 86
Cuts at: 5842 5854 6200 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 920 440 385 367 346 203
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2211 2292 3066 513
Size: 552 1146 81 774 3652
Fragments arranged by size:
3652 1146 774 552 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 3721 4515 4535 3721
Size: 794 20 5391
Fragments arranged by size:
5391 794 20
ClaI AT'CG_AT
Cuts at: 1554 2430 2445 1554
Size: 876 15 5314
Fragments arranged by size:
5314 876 15
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DraIII CAC_nnn'GTG
Cuts at: 3027 3027
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 2247 2362
Size: 54 1425 50 275 18 378 39 115
Cuts at: 2362 3652 4541 6170 8
Size: 1290 889 1629 43
Fragments arranged by size:
1629 1425 1290 889 378 275 115 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2362 3652 1537
Size: 275 550 1290 4090
Fragments arranged by size:
4090 1290 550 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2877 4135 1353
Size: 1524 1258 3423
Fragments arranged by size:
3423 1524 1258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2437
Size: 6205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2455 2624 2759 2936 4268 4865
Size: 393 656 167 169 135 177 1332 597
Cuts at: 4865 5652 5920 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1332 787 656 597 393 268 177
169 167 135
EcoNI CCTnn'n_nnAGG
Cuts at: 2455 2819 2455
Size: 364 5841
EcoO109I rG'GnC_Cy
Cuts at: 2241 2242 2564 2241
Size: 1 322 5882
Fragments arranged by size:
5882 322 1
EcoRI G'AATT_C
Cuts at: 3252 3514 3892 3252
Size: 262 378 5565
Fragments arranged by size:
5565 378 262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3159 3159
Size: 6205
FspI TGC'GCA
Cuts at: 2966 4711 2966
Size: 1745 4460
HaeII r_GCGC'y
Cuts at: 1678 2027 2114 2174 5352 1678
Size: 349 87 60 3178 2531
Fragments arranged by size:
3178 2531 349 87 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2306 2338 3281
Size: 32 135 32 464 32 238 32 943
Cuts at: 3281 3313 4846 4878 4920 4952 1373
Size: 32 1533 32 42 32 2626
Fragments arranged by size:
2626 1533 943 464 238 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 2534 3403 3549 3795 669
Size: 988 281 196 400 869 146 246 3079
Fragments arranged by size:
3079 988 869 400 281 246 196 146
HindIII A'AGCT_T
Cuts at: 748 3362 748
Size: 2614 3591
HpaI GTT'AAC
Cuts at: 3403 3795 3403
Size: 392 5813
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5319 5503 1305
Size: 4014 184 2007
Fragments arranged by size:
4014 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4383
Fragments arranged by size:
4383 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 2681 3154 3170 3437 4163 4522
Size: 648 1375 140 473 16 267 726 359
Cuts at: 4522 4681 518
Size: 159 2042
Fragments arranged by size:
2042 1375 726 648 473 359 267 159
140 16
MspA1I CmG'CkG
Cuts at: 1606 2016 2041 2049 2386 2627 2699 2725 3335
Size: 410 25 8 337 241 72 26 610
Cuts at: 3335 3483 3621 4298 5446 5691 1606
Size: 148 138 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 610 410 337 245 241
148 138 72 26 25 8
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5653
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2375 2971 3563 5108 1132
Size: 97 1146 596 592 1545 2229
Fragments arranged by size:
2229 1545 1146 596 592 97
PasI CC'CwG_GG
Cuts at: 1348 2065 1348
Size: 717 5488
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6205
PfoI T'CCnGG_A
Cuts at: 2601 2736 2601
Size: 135 6070
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2564 2564
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 2805 1613
Size: 1192 5013
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 2241 2241
Size: 6205
PstI C_TGCA'G
Cuts at: 830 2087 2409 2459 2724 3557 830
Size: 1257 322 50 265 833 3478
Fragments arranged by size:
3478 1257 833 322 265 50
PvuI CG_AT'CG
Cuts at: 2001 3292 2001
Size: 1291 4914
PvuII CAG'CTG
Cuts at: 2386 2627 2699 2725 3335 2386
Size: 241 72 26 610 5256
Fragments arranged by size:
5256 610 241 72 26
SacI G_AGCT'C
Cuts at: 2439 2439
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 2409 3557 2409
Size: 1148 5057
ScaI AGT'ACT
Cuts at: 2306 2420 3472 4453 6165 2306
Size: 114 1052 981 1712 2346
Fragments arranged by size:
2346 1712 1052 981 114
SfcI C'TryA_G
Cuts at: 826 1045 2083 2405 2455 2720 3090 3553 4688
Size: 219 1038 322 50 265 370 463 1135
Cuts at: 4688 5369 5560 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1038 681 463 370 322 265
219 191 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 2239 3532 1872
Size: 367 1293 4545
Fragments arranged by size:
4545 1293 367
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3323 3406 4309 5210 5472 820
Size: 197 926 1380 83 903 901 262 1553
Fragments arranged by size:
1553 1380 926 903 901 262 197 83
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 2971 3563 1132
Size: 1839 592 3774
Fragments arranged by size:
3774 1839 592
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2106 2197 2560 3007 3644 513
Size: 552 1041 91 363 447 637 3074
Fragments arranged by size:
3074 1041 637 552 447 363 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3038 4232 4249 4402 4587 5032 325
Size: 1255 1458 1194 17 153 185 445 1498
Fragments arranged by size:
1498 1458 1255 1194 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2418 3470
Size: 274 80 33 51 1443 327 114 1052
Cuts at: 3470 4451 6163 96
Size: 981 1712 138
Fragments arranged by size:
1712 1443 1052 981 327 274 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2732 2764 6165 6197 2372
Size: 32 328 32 3401 32 2380
Fragments arranged by size:
3401 2380 328 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2568 1316
Size: 1252 4953
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 2680 2911 2659
Size: 21 231 5953
Fragments arranged by size:
5953 231 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 2237 3530 1870
Size: 367 1293 4545
Fragments arranged by size:
4545 1293 367
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI