Restriction map for
pFN21AE3926
(Circular) (Six-base) MAPSORT of: pFN21AE3926.seq Check: 5523 from: 1 to: 6358
pFN21AE3926 6358 bp
With 182 enzymes: SgfI *
March 28, 2012 11:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2379 2473 2379
Size: 94 6264
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3179 4168 278
Size: 53 83 186 936 1643 989 2468
Fragments arranged by size:
2468 1643 989 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3701 1937
Size: 1764 4594
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 2708 3043 4421 5805 6073 1632
Size: 599 477 335 1378 1384 268 1917
Fragments arranged by size:
1917 1384 1378 599 477 335 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 5257 6234 1203
Size: 4054 977 1327
Fragments arranged by size:
4054 1327 977
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 3068 1166
Size: 1902 4456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3086 1184
Size: 1902 4456
AlwNI CAG_nnn'CTG
Cuts at: 2731 2812 3250 3573 4101 5673 2731
Size: 81 438 323 528 1572 3416
Fragments arranged by size:
3416 1572 528 438 323 81
ApaI G_GGCC'C
Cuts at: 3188 3258 3188
Size: 70 6288
ApaLI G'TGCA_C
Cuts at: 3308 4415 5571 3308
Size: 1107 1156 4095
Fragments arranged by size:
4095 1156 1107
ApoI r'AATT_y
Cuts at: 1493 3667 3893 4045 6154 1493
Size: 2174 226 152 2109 1697
Fragments arranged by size:
2174 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 2819 3683 1870
Size: 73 702 174 864 4545
Fragments arranged by size:
4545 864 702 174 73
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2338 2397 3070 3296 3679
Size: 325 217 627 551 59 673 226 383
Cuts at: 3679 5134 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 673 627 551 383 325 226
217 59
BanII G_rGCy'C
Cuts at: 1391 2043 2858 3188 3258 3677 1391
Size: 652 815 330 70 419 4072
Fragments arranged by size:
4072 815 652 419 330 70
BbeI G_GCGC'C
Cuts at: 2342 3300 2342
Size: 958 5400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3382 3416 4549 4583 1903
Size: 34 1445 34 1133 34 3678
Fragments arranged by size:
3678 1445 1133 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3351 4250 5460 3351
Size: 899 1210 4249
Fragments arranged by size:
4249 1210 899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 3192 3253
Size: 107 122 71 631 605 623 897 61
Cuts at: 3253 4969 136
Size: 1716 1525
Fragments arranged by size:
1716 1525 897 631 623 605 122 107
71 61
BglII A'GATC_T
Cuts at: 6353 6353
Size: 6358
BlpI GC'TnA_GC
Cuts at: 3775 3775
Size: 6358
Bme1580I G_kGCm'C
Cuts at: 1790 2400 3075 3188 3218 3258 3312 3498 4419
Size: 610 675 113 30 40 54 186 921
Cuts at: 4419 5575 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 921 675 610 186 113 54
40 30
BmgBI CAC'GTC
Cuts at: 1318 2422 2548 1318
Size: 1104 126 5128
Fragments arranged by size:
5128 1104 126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3373 3538 4089
Size: 416 53 83 399 24 1956 165 551
Cuts at: 4089 5047 442
Size: 958 1753
Fragments arranged by size:
1956 1753 958 551 416 399 165 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2452 2624 5018 1239
Size: 980 233 172 2394 2579
Fragments arranged by size:
2579 2394 980 233 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4483 5348 5646 3298
Size: 1185 865 298 4010
Fragments arranged by size:
4010 1185 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5021 882
Size: 617 3522 2219
Fragments arranged by size:
3522 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2251 2359 3649 4149 493
Size: 711 1047 108 1290 500 2702
Fragments arranged by size:
2702 1290 1047 711 500 108
BsaBI GATnn'nnATC
Cuts at: 6352 6352
Size: 6358
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2339 3176 3297 3582
Size: 53 83 186 936 806 837 121 285
Cuts at: 3582 4165 4547 5182 275
Size: 583 382 635 1451
Fragments arranged by size:
1451 936 837 806 635 583 382 285
186 121 83 53
BsaWI w'CCGG_w
Cuts at: 2571 4791 5189 5463 5610 6021 2571
Size: 2220 398 274 147 411 2908
Fragments arranged by size:
2908 2220 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2975 3005 3455 3485 3504
Size: 30 165 30 1572 30 450 30 19
Cuts at: 3504 3534 1178
Size: 30 4002
Fragments arranged by size:
4002 1572 450 165 30 30 30 30
30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2051 2621 2684 1186
Size: 862 3 570 63 4860
Fragments arranged by size:
4860 862 570 63 3
BseYI C'CCAG_C
Cuts at: 2851 3360 3554 3588 5561 6057 2851
Size: 509 194 34 1973 496 3152
Fragments arranged by size:
3152 1973 509 496 194 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2102 2231 2258 2381 2705 2798
Size: 573 309 57 129 27 123 324 93
Cuts at: 2798 2932 1163
Size: 134 4589
Fragments arranged by size:
4589 573 324 309 134 129 123 93
57 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3391 3808 4569 5597 1540
Size: 275 186 1390 417 761 1028 2301
Fragments arranged by size:
2301 1390 1028 761 417 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 3312 4419 4504 5575 2043
Size: 1269 1107 85 1071 2826
Fragments arranged by size:
2826 1269 1107 1071 85
BsiWI C'GTAC_G
Cuts at: 2152 2152
Size: 6358
BsmI GAATG_Cn'
Cuts at: 3869 3962 3869
Size: 93 6265
BsmBI CGTCTCn'nnnn_
Cuts at: 3167 3167
Size: 6358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2400 2858 3075 3188 3218 3258
Size: 399 253 357 458 217 113 30 40
Cuts at: 3258 3312 3498 3677 4419 4504 5575 1391
Size: 54 186 179 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 357 253 217
186 179 113 85 54 40 30
BspEI T'CCGG_A
Cuts at: 2571 6021 2571
Size: 3450 2908
BspHI T'CATG_A
Cuts at: 2583 2898 4247 5977 2583
Size: 315 1349 1730 2964
Fragments arranged by size:
2964 1730 1349 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2379 2473 2751 2954 3713 844
Size: 1535 94 278 203 759 3489
Fragments arranged by size:
3489 1535 759 278 203 94
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 3520 4245 1394
Size: 429 1648 49 725 3507
Fragments arranged by size:
3507 1648 725 429 49
BsrDI GCAATG_nn'
Cuts at: 65 1248 4853 5027 65
Size: 1183 3605 174 1396
Fragments arranged by size:
3605 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2263 2598 3067 5002 5189 1700
Size: 110 453 335 469 1935 187 2869
Fragments arranged by size:
2869 1935 469 453 335 187 110
BsrGI T'GTAC_A
Cuts at: 96 2637 96
Size: 2541 3817
BssHII G'CGCG_C
Cuts at: 1925 2520 1925
Size: 595 5763
BssSI C'ACGA_G
Cuts at: 2950 4418 5430 2950
Size: 1468 1012 3878
Fragments arranged by size:
3878 1468 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2728 2812 3370 794
Size: 1934 84 558 3782
Fragments arranged by size:
3782 1934 558 84
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2227
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2553 3688 4055 4440 4457 5909 5995 6007
Size: 581 1135 367 385 17 1452 86 12
Cuts at: 6007 6353 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1135 581 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2293 2392 3435 3445 513
Size: 552 939 289 99 1043 10 3426
Fragments arranged by size:
3426 1043 939 552 289 99 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3318 487
Size: 683 56 2092 3527
Fragments arranged by size:
3527 2092 683 56
BtsI GCAGTG_nn'
Cuts at: 2275 2467 3874 4668 4688 2275
Size: 192 1407 794 20 3945
Fragments arranged by size:
3945 1407 794 192 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 3662 3662
Size: 6358
DraIII CAC_nnn'GTG
Cuts at: 2281 2539 3280 3649 2281
Size: 258 741 369 4990
Fragments arranged by size:
4990 741 369 258
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2487 2980 3015
Size: 54 1425 50 275 18 657 493 35
Cuts at: 3015 3196 3388 3438 3451 3475 3598 3805 4694
Size: 181 192 50 13 24 123 207 889
Cuts at: 4694 6323 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 657 493 275 207 192
181 123 54 50 50 43 35 24
18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3388 3805 1537
Size: 275 1576 417 4090
Fragments arranged by size:
4090 1576 417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3005 4288 1353
Size: 1652 1283 3423
Fragments arranged by size:
3423 1652 1283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2429 3140 3343 4945 5319
Size: 1064 138 435 405 711 203 1602 374
Cuts at: 5319 5465 387
Size: 146 1280
Fragments arranged by size:
1602 1280 1064 711 435 405 374 203
146 138
EcoICRI GAG'CTC
Cuts at: 2041 2041
Size: 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2231 2452 2624 2708 3043 4421
Size: 393 587 12 221 172 84 335 1378
Cuts at: 4421 5018 5805 6073 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1378 787 597 587 393 335 268
221 172 84 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2408 2666 2768 2408
Size: 258 102 5998
Fragments arranged by size:
5998 258 102
EcoO109I rG'GnC_Cy
Cuts at: 2740 2761 2937 3053 3185 3254 3255 2740
Size: 21 176 116 132 69 1 5843
Fragments arranged by size:
5843 176 132 116 69 21 1
EcoRI G'AATT_C
Cuts at: 3667 4045 3667
Size: 378 5980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 6358
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 2273 2342 2662 2813 3300 5505 1678
Size: 595 69 320 151 487 2205 2531
Fragments arranged by size:
2531 2205 595 487 320 151 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3164 3196 3221 3253 4999
Size: 32 135 32 1592 32 25 32 1746
Cuts at: 4999 5031 5073 5105 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1746 1592 135 42 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3702 3948 669
Size: 988 281 1764 246 3079
Fragments arranged by size:
3079 1764 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KasI G'GCGC_C
Cuts at: 2338 3296 2338
Size: 958 5400
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5472 5656 1305
Size: 4167 184 2007
Fragments arranged by size:
4167 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 3440 10
Size: 54 1425 343 1185 423 2928
Fragments arranged by size:
2928 1425 1185 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3068 4316 4675 4834 518
Size: 648 1902 1248 359 159 2042
Fragments arranged by size:
2042 1902 1248 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2145 2295 2305 2394 2517 2635 2728
Size: 480 59 150 10 89 123 118 93
Cuts at: 2728 2866 3034 3225 3774 4451 5599 5844 1606
Size: 138 168 191 549 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 549 480 245 191 168
150 138 123 118 93 89 59 10
NaeI GCC'GGC
Cuts at: 2265 2265
Size: 6358
NarI GG'CG_CC
Cuts at: 2339 3297 2339
Size: 958 5400
NcoI C'CATG_G
Cuts at: 513 1065 2004 3445 513
Size: 552 939 1441 3426
Fragments arranged by size:
3426 1441 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NgoMIV G'CCGG_C
Cuts at: 2263 2263
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3388 3805 3388
Size: 417 5941
NspI r_CATG'y
Cuts at: 1132 1229 2833 3716 5261 1132
Size: 97 1604 883 1545 2229
Fragments arranged by size:
2229 1604 1545 883 97
PasI CC'CwG_GG
Cuts at: 1348 3250 1348
Size: 1902 4456
PciI A'CATG_T
Cuts at: 5257 5257
Size: 6358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 1108
Size: 1623 4735
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PmlI CAC'GTG
Cuts at: 2251 2359 2251
Size: 108 6250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2740 2761 2937 2740
Size: 21 176 6161
Fragments arranged by size:
6161 176 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 3928 3928
Size: 6358
PspOMI G'GGCC_C
Cuts at: 3184 3254 3184
Size: 70 6288
PspXI vC'TCGA_Gb
Cuts at: 2645 2645
Size: 6358
PstI C_TGCA'G
Cuts at: 830 2376 2709 2792 2865 3084 3263 3710 830
Size: 1546 333 83 73 219 179 447 3478
Fragments arranged by size:
3478 1546 447 333 219 179 83 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
PvuII CAG'CTG
Cuts at: 2086 2635 2728 2866 3034 2086
Size: 549 93 138 168 5410
Fragments arranged by size:
5410 549 168 138 93
SacI G_AGCT'C
Cuts at: 2043 2043
Size: 6358
SacII CC_GC'GG
Cuts at: 2296 2395 2296
Size: 99 6259
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SapI GCTCTTCn'nnn_
Cuts at: 3005 3005
Size: 6358
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 2501 3509 4606 6318 2501
Size: 1008 1097 1712 2541
Fragments arranged by size:
2541 1712 1097 1008
SfcI C'TryA_G
Cuts at: 826 1045 2372 2512 2705 2788 2861 3080 3259
Size: 219 1327 140 193 83 73 219 179
Cuts at: 3259 3613 3706 4841 5522 5713 826
Size: 354 93 1135 681 191 1471
Fragments arranged by size:
1471 1327 1135 681 354 219 219 193
191 179 140 93 83 73
SfoI GGC'GCC
Cuts at: 2340 3298 2340
Size: 958 5400
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 2263 3067 1700
Size: 563 804 4991
Fragments arranged by size:
4991 804 563
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2645 3313 4462 5363 5625 820
Size: 197 926 702 668 1149 901 262 1553
Fragments arranged by size:
1553 1149 926 901 702 668 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 2833 3716 1132
Size: 1701 883 3774
Fragments arranged by size:
3774 1701 883
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 2177 1517
Size: 660 5698
StyI C'CwwG_G
Cuts at: 513 1065 2004 2136 3445 3797 513
Size: 552 939 132 1309 352 3074
Fragments arranged by size:
3074 1309 939 552 352 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4385 4402 4555 4740 5185 325
Size: 1255 2805 17 153 185 445 1498
Fragments arranged by size:
2805 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 2637 3507
Size: 274 80 33 51 1443 522 138 870
Cuts at: 3507 4604 6316 96
Size: 1097 1712 138
Fragments arranged by size:
1712 1443 1097 870 522 274 138 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2523 2555 2975 3007 3504 3522 3536 3554 6318
Size: 32 420 32 497 18 14 18 2764
Cuts at: 6318 6350 2523
Size: 32 2531
Fragments arranged by size:
2764 2531 497 420 32 32 32 18
18 14
Tth111I GACn'n_nGTC
Cuts at: 1316 3178 1316
Size: 1862 4496
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2848 3274 2848
Size: 426 5932
XhoI C'TCGA_G
Cuts at: 1943 2645 1943
Size: 702 5656
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 2027 4487 2027
Size: 2460 3898
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3177 4166 276
Size: 53 83 186 936 1643 989 2468
Fragments arranged by size:
2468 1643 989 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PfoI PsrI RsrII SanDI SexAI SfiI SrfI
SwaI