Restriction map for
pFN21AE3925
(Circular) (Six-base) MAPSORT of: pFN21AE3925.seq Check: 2988 from: 1 to: 5374
pFN21AE3925 5374 bp
With 182 enzymes: SgfI *
March 28, 2012 11:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2177 2695 2177
Size: 518 4856
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 2033 2077 2376 2454 2512 3117 4689 2033
Size: 44 299 78 58 605 1572 2718
Fragments arranged by size:
2718 1572 605 299 78 58 44
ApaI G_GGCC'C
Cuts at: 2210 2233 2440 2210
Size: 23 207 5144
Fragments arranged by size:
5144 207 23
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2699 1870
Size: 73 756 4545
Fragments arranged by size:
4545 756 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 2695 4150 618
Size: 325 217 627 390 518 1455 1842
Fragments arranged by size:
1842 1455 627 518 390 325 217
BanII G_rGCy'C
Cuts at: 1391 2098 2210 2233 2440 2693 1391
Size: 707 112 23 207 253 4072
Fragments arranged by size:
4072 707 253 207 112 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BbvCI CC'TCA_GC
Cuts at: 2117 2309 2117
Size: 192 5182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 136
Size: 107 122 71 631 605 2313 1525
Fragments arranged by size:
2313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2210 2233 2440 2570 3435 4591 1790
Size: 420 23 207 130 865 1156 2573
Fragments arranged by size:
2573 1156 865 420 207 130 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2279 2288 2408 4034 1239
Size: 941 99 9 120 1626 2579
Fragments arranged by size:
2579 1626 941 120 99 9
Bpu10I CC'TnA_GC
Cuts at: 2117 2309 2324 2117
Size: 192 15 5167
Fragments arranged by size:
5167 192 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2565 3165 493
Size: 711 1361 600 2702
Fragments arranged by size:
2702 1361 711 600
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2187 3181 3563 4198
Size: 53 83 186 936 654 994 382 635
Cuts at: 4198 275
Size: 1451
Fragments arranged by size:
1451 994 936 654 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5374
BseYI C'CCAG_C
Cuts at: 2103 2433 2607 4577 5073 2103
Size: 330 174 1970 496 2404
Fragments arranged by size:
2404 1970 496 330 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2554 1163
Size: 573 818 3983
Fragments arranged by size:
3983 818 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 2394 3435 3520 4591 2295
Size: 99 1041 85 1071 3078
Fragments arranged by size:
3078 1071 1041 99 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2210 2233 2295 2394 2440 2570
Size: 399 308 112 23 62 99 46 130
Cuts at: 2570 2693 3435 3520 4591 1391
Size: 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 130 123 112
99 85 62 46 23
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3463
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2033 794
Size: 1239 4135
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2173 2173
Size: 5374
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2128 2407 2890 3684 3704 2128
Size: 279 483 794 20 3798
Fragments arranged by size:
3798 794 483 279 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2367 2456
Size: 54 1425 50 275 18 220 317 89
Cuts at: 2456 2559 2583 2821 3710 5339 8
Size: 103 24 238 889 1629 43
Fragments arranged by size:
1629 1425 889 317 275 238 220 103
89 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2140 3304 1353
Size: 787 1164 3423
Fragments arranged by size:
3423 1164 787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2279 2288 2408 3437 4034 4821
Size: 393 548 99 9 120 1029 597 787
Cuts at: 4821 5089 1239
Size: 268 1524
Fragments arranged by size:
1524 1029 787 597 548 393 268 120
99 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2458 2458
Size: 5374
EcoO109I rG'GnC_Cy
Cuts at: 2074 2206 2207 2230 2074
Size: 132 1 23 5218
Fragments arranged by size:
5218 132 23 1
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2402 4521 1678
Size: 724 2119 2531
Fragments arranged by size:
2531 2119 724
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373
Size: 32 135 32 2443 32 42 32 2626
Fragments arranged by size:
2626 2443 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KpnI G_GTAC'C
Cuts at: 2181 2699 2181
Size: 518 4856
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2223 2532 4488 4672 1305
Size: 918 309 1956 184 2007
Fragments arranged by size:
2007 1956 918 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2369 2458 2561 10
Size: 54 1425 343 537 89 103 2823
Fragments arranged by size:
2823 1425 537 343 103 89 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2579 3332 3691 3850 518
Size: 648 1413 753 359 159 2042
Fragments arranged by size:
2042 1413 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2308 2317 2487 2611 2790 3467 4615
Size: 501 201 9 170 124 179 677 1148
Cuts at: 4615 4860 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 501 245 201 179 170
124 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 4645
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2077 2454 1108
Size: 969 377 4028
Fragments arranged by size:
4028 969 377
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PmlI CAC'GTG
Cuts at: 2565 2565
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2190 1613
Size: 577 4797
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PspOMI G'GGCC_C
Cuts at: 2206 2229 2436 2206
Size: 23 207 5144
Fragments arranged by size:
5144 207 23
PstI C_TGCA'G
Cuts at: 830 2029 2038 2238 2261 2414 2469 2492 2726
Size: 1199 9 200 23 153 55 23 234
Cuts at: 2726 830
Size: 3478
Fragments arranged by size:
3478 1199 234 200 153 55 23 23
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
PvuII CAG'CTG
Cuts at: 2107 2308 2317 2487 2611 2107
Size: 201 9 170 124 4870
Fragments arranged by size:
4870 201 170 124 9
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SapI GCTCTTCn'nnn_
Cuts at: 2140 2140
Size: 5374
SbfI CC_TGCA'GG
Cuts at: 2029 2238 2726 2029
Size: 209 488 4677
Fragments arranged by size:
4677 488 209
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2025 2034 2234 2257 2410 2465 2488
Size: 219 980 9 200 23 153 55 23
Cuts at: 2488 2722 3857 4538 4729 826
Size: 234 1135 681 191 1471
Fragments arranged by size:
1471 1135 980 681 234 219 200 191
153 55 23 23 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 2171 1517
Size: 654 4720
StyI C'CwwG_G
Cuts at: 513 1065 2181 2286 2459 2813 513
Size: 552 1116 105 173 354 3074
Fragments arranged by size:
3074 1116 552 354 173 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2007 3401 3418 3571 3756 4201 325
Size: 1255 427 1394 17 153 185 445 1498
Fragments arranged by size:
1498 1394 1255 445 427 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3620 5332
Size: 274 80 33 51 1443 30 1613 1712
Cuts at: 5332 96
Size: 138
Fragments arranged by size:
1712 1613 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5334 5366 5334
Size: 32 5342
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI