Restriction map for
pFN21AE3923
(Circular) (Six-base) MAPSORT of: pFN21AE3923.seq Check: 5704 from: 1 to: 6046
pFN21AE3923 6046 bp
With 182 enzymes: SgfI *
March 28, 2012 11:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3163 3163
Size: 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2422 2521 3076 3389 1937
Size: 485 99 555 313 4594
Fragments arranged by size:
4594 555 485 313 99
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2684 4109 5493 5761 1632
Size: 1052 1425 1384 268 1917
Fragments arranged by size:
1917 1425 1384 1052 268
AfeI AGC'GCT
Cuts at: 2345 2345
Size: 6046
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 2368 2773 4945 5922 1203
Size: 1165 405 2172 977 1327
Fragments arranged by size:
2172 1327 1165 977 405
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 1184
Size: 1179 4867
AlwNI CAG_nnn'CTG
Cuts at: 3789 5361 3789
Size: 1572 4474
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 2506 2686 2927 3086 3331 3355 3581 3733
Size: 1013 180 241 159 245 24 226 152
Cuts at: 3733 5842 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1013 245 241 226 180 159
152 24
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 3371 1870
Size: 73 1428 4545
Fragments arranged by size:
4545 1428 73
AvrII C'CTAG_G
Cuts at: 2312 2312
Size: 6046
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 2088 3367 4822 618
Size: 325 217 627 226 75 1279 1455 1842
Fragments arranged by size:
1842 1455 1279 627 325 226 217 75
BanII G_rGCy'C
Cuts at: 1391 2312 3365 1391
Size: 921 1053 4072
Fragments arranged by size:
4072 1053 921
BbeI G_GCGC'C
Cuts at: 2017 2092 2017
Size: 75 5971
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2218 2439 928
Size: 971 319 221 4535
Fragments arranged by size:
4535 971 319 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 3052 1548
Size: 1504 4542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 4107 5263 1790
Size: 2317 1156 2573
Fragments arranged by size:
2573 2317 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3777 4735 442
Size: 416 53 83 399 24 2360 958 1753
Fragments arranged by size:
2360 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 4706 1239
Size: 1280 2187 2579
Fragments arranged by size:
2579 2187 1280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 4709 882
Size: 617 909 2301 2219
Fragments arranged by size:
2301 2219 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 2089 3853 4235
Size: 53 83 186 936 481 75 1764 382
Cuts at: 4235 4870 275
Size: 635 1451
Fragments arranged by size:
1764 1451 936 635 481 382 186 83
75 53
BsaWI w'CCGG_w
Cuts at: 2177 4479 4877 5151 5298 5709 2177
Size: 2302 398 274 147 411 2514
Fragments arranged by size:
2514 2302 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5821
Fragments arranged by size:
5821 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 3266 1186
Size: 1330 750 3966
Fragments arranged by size:
3966 1330 750
BseYI C'CCAG_C
Cuts at: 2066 5249 5745 2066
Size: 3183 496 2367
Fragments arranged by size:
3183 2367 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5473
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2840 4107 4192 5263 2840
Size: 1267 85 1071 3623
Fragments arranged by size:
3623 1267 1071 85
BsmI GAATG_Cn'
Cuts at: 2632 2876 3557 3650 2632
Size: 244 681 93 5028
Fragments arranged by size:
5028 681 244 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 2840 3365 4107 4192 5263 1391
Size: 399 522 528 525 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 525 522 399 85
BspEI T'CCGG_A
Cuts at: 2177 5709 2177
Size: 3532 2514
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3163 3197 3401 844
Size: 2319 34 204 3489
Fragments arranged by size:
3489 2319 204 34
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 4690 4877 1700
Size: 110 200 2680 187 2869
Fragments arranged by size:
2869 2680 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 2886 96
Size: 2790 3256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 2758 4850 2758
Size: 2092 3954
BstEII G'GTnAC_C
Cuts at: 1184 2295 1184
Size: 1111 4935
BstYI r'GATC_y
Cuts at: 1972 2853 3376 3743 4128 4145 5597 5683 5695
Size: 881 523 367 385 17 1452 86 12
Cuts at: 5695 6041 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 881 523 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 2079 3562 4356 4376 2079
Size: 1483 794 20 3749
Fragments arranged by size:
3749 1483 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 3493 4382
Size: 54 1425 50 275 18 206 1457 889
Cuts at: 4382 6011 8
Size: 1629 43
Fragments arranged by size:
1629 1457 1425 889 275 206 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3976 1353
Size: 2623 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2519 2684 4109 4706 5493 5761 1239
Size: 393 887 165 1425 597 787 268 1524
Fragments arranged by size:
1524 1425 887 787 597 393 268 165
EcoNI CCTnn'n_nnAGG
Cuts at: 2498 2498
Size: 6046
EcoO109I rG'GnC_Cy
Cuts at: 3299 3299
Size: 6046
EcoRI G'AATT_C
Cuts at: 3086 3355 3733 3086
Size: 269 378 5399
Fragments arranged by size:
5399 378 269
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 2017 2061 2092 2347 5193 1678
Size: 339 44 31 255 2846 2531
Fragments arranged by size:
2846 2531 339 255 44 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4687 4719 4761 4793 1373
Size: 32 135 32 3115 32 42 32 2626
Fragments arranged by size:
3115 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3077 3390 3636 669
Size: 988 281 1139 313 246 3079
Fragments arranged by size:
3079 1139 988 313 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KasI G'GCGC_C
Cuts at: 2013 2088 2013
Size: 75 5971
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 5344 1305
Size: 3855 184 2007
Fragments arranged by size:
3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4224
Fragments arranged by size:
4224 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4004 4363 4522 518
Size: 648 2838 359 159 2042
Fragments arranged by size:
2838 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2046 3462 4139 5287 5532 1606
Size: 419 21 1416 677 1148 245 2120
Fragments arranged by size:
2120 1416 1148 677 419 245 21
NaeI GCC'GGC
Cuts at: 2012 2012
Size: 6046
NarI GG'CG_CC
Cuts at: 2014 2089 2014
Size: 75 5971
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5494
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NgoMIV G'CCGG_C
Cuts at: 2010 2010
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 2372 2777 3404 4949 1132
Size: 97 1143 405 627 1545 2229
Fragments arranged by size:
2229 1545 1143 627 405 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6046
PciI A'CATG_T
Cuts at: 2368 2773 4945 2368
Size: 405 2172 3469
Fragments arranged by size:
3469 2172 405
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PfoI T'CCnGG_A
Cuts at: 2496 2496
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3299 3299
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2024 2057 2700 3398 830
Size: 1194 33 643 698 3478
Fragments arranged by size:
3478 1194 698 643 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2025 2025
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3075 3388 1936
Size: 1139 313 4594
Fragments arranged by size:
4594 1139 313
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2020 2053 2696 3394 4529 5210 5401
Size: 219 975 33 643 698 1135 681 191
Cuts at: 5401 826
Size: 1471
Fragments arranged by size:
1471 1135 975 698 681 643 219 191
33
SfoI GGC'GCC
Cuts at: 2015 2090 2015
Size: 75 5971
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 2215 152
Size: 2063 3983
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6046
StyI C'CwwG_G
Cuts at: 513 1065 2312 3485 513
Size: 552 1247 1173 3074
Fragments arranged by size:
3074 1247 1173 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2390 2886 4292
Size: 274 80 33 51 1443 413 496 1406
Cuts at: 4292 6004 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1406 496 413 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6006 6038 6006
Size: 32 6014
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 2754 4175 2754
Size: 1421 4625
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI