Restriction map for
pFN21AE3913
(Circular) (Six-base) MAPSORT of: pFN21AE3913.seq Check: 2388 from: 1 to: 5335
pFN21AE3913 5335 bp
With 182 enzymes: SgfI *
March 28, 2012 11:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2099 3145 278
Size: 53 83 186 936 563 1046 2468
Fragments arranged by size:
2468 1046 936 563 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2337 2368 2656 2337
Size: 31 288 5016
Fragments arranged by size:
5016 288 31
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 4782 5050 1632
Size: 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 4234 5211 1203
Size: 3031 977 1327
Fragments arranged by size:
3031 1327 977
AgeI A'CCGG_T
Cuts at: 2331 4166 2331
Size: 1835 3500
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2213 1184
Size: 1029 4306
AlwNI CAG_nnn'CTG
Cuts at: 3078 4650 3078
Size: 1572 3763
ApaLI G'TGCA_C
Cuts at: 2198 3392 4548 2198
Size: 1194 1156 2985
Fragments arranged by size:
2985 1194 1156
ApoI r'AATT_y
Cuts at: 1493 2644 2870 3022 5131 1493
Size: 1151 226 152 2109 1697
Fragments arranged by size:
2109 1697 1151 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2132 2510 2660 1870
Size: 73 189 378 150 4545
Fragments arranged by size:
4545 378 189 150 73
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2337 2368 2656 4111
Size: 325 217 627 248 302 31 288 1455
Cuts at: 4111 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 302 288 248 217
31
BanII G_rGCy'C
Cuts at: 1391 2654 1391
Size: 1263 4072
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 5335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 2104 2542 928
Size: 971 200 5 438 3721
Fragments arranged by size:
3721 971 438 200 5
BbvCI CC'TCA_GC
Cuts at: 2576 2576
Size: 5335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3526 3560 1903
Size: 34 1589 34 3678
Fragments arranged by size:
3678 1589 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2123 2570
Size: 107 122 71 631 605 337 114 447
Cuts at: 2570 3946 136
Size: 1376 1525
Fragments arranged by size:
1525 1376 631 605 447 337 122 114
107 71
BglII A'GATC_T
Cuts at: 5330 5330
Size: 5335
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2202 3396 4552 1790
Size: 412 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 412
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3995 1239
Size: 2756 2579
Bpu10I CC'TnA_GC
Cuts at: 2576 2576
Size: 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 882
Size: 617 2499 2219
Fragments arranged by size:
2499 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 2096 2268 3142
Size: 53 83 186 936 503 60 172 874
Cuts at: 3142 3524 4159 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 874 635 503 382 186 172
83 60 53
BsaWI w'CCGG_w
Cuts at: 2331 3768 4166 4440 4587 4998 2331
Size: 1437 398 274 147 411 2668
Fragments arranged by size:
2668 1437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5110
Fragments arranged by size:
5110 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 1186
Size: 859 4476
BseYI C'CCAG_C
Cuts at: 4538 5034 4538
Size: 496 4839
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4762
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2590 2785 3546 4574 1540
Size: 275 186 589 195 761 1028 2301
Fragments arranged by size:
2301 1028 761 589 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2519 3396 3481 4552 2202
Size: 317 877 85 1071 2985
Fragments arranged by size:
2985 1071 877 317 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2519 2654 3396 3481 4552 1391
Size: 399 412 317 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 317 135 85
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2409 2690 844
Size: 1340 225 281 3489
Fragments arranged by size:
3489 1340 281 225
BsrBI CCG'CTC
Cuts at: 1394 1823 2161 3222 1394
Size: 429 338 1061 3507
Fragments arranged by size:
3507 1061 429 338
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2331 3979 4166 1700
Size: 110 521 1648 187 2869
Fragments arranged by size:
2869 1648 521 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 2629 1184
Size: 1445 3890
BstYI r'GATC_y
Cuts at: 1972 2665 3032 3417 3434 4886 4972 4984 5330
Size: 693 367 385 17 1452 86 12 346
Cuts at: 5330 1972
Size: 1977
Fragments arranged by size:
1977 1452 693 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2148 2490 2584 513
Size: 552 957 126 342 94 3264
Fragments arranged by size:
3264 957 552 342 126 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3645 3665 2851
Size: 794 20 4521
Fragments arranged by size:
4521 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2137 2172 529
Size: 35 1573 35 3692
Fragments arranged by size:
3692 1573 35 35
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2273 4342 809
Size: 507 957 2069 1802
Fragments arranged by size:
2069 1802 957 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2782 3671 5300
Size: 54 1425 50 275 18 952 889 1629
Cuts at: 5300 8
Size: 43
Fragments arranged by size:
1629 1425 952 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2620 3922 4296 4442 387
Size: 1064 138 1031 1302 374 146 1280
Fragments arranged by size:
1302 1280 1064 1031 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3398 3995 4782 5050 1239
Size: 393 1766 597 787 268 1524
Fragments arranged by size:
1766 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2405 2464 2405
Size: 59 5276
EcoO109I rG'GnC_Cy
Cuts at: 2124 2124
Size: 5335
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 2405 3841 2405
Size: 1436 3899
HaeII r_GCGC'y
Cuts at: 1678 2039 4482 1678
Size: 361 2443 2531
Fragments arranged by size:
2531 2443 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3976 4008 4050 4082 1373
Size: 32 135 32 2404 32 42 32 2626
Fragments arranged by size:
2626 2404 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 2679 2925 669
Size: 988 281 235 506 246 3079
Fragments arranged by size:
3079 988 506 281 246 235
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 5335
KpnI G_GTAC'C
Cuts at: 2341 2372 2660 2341
Size: 31 288 5016
Fragments arranged by size:
5016 288 31
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 3293 3652 3811 518
Size: 648 1215 912 359 159 2042
Fragments arranged by size:
2042 1215 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2586 2751 3428 4576 4821 1606
Size: 418 562 165 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 562 418 245 165
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 5335
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4783
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2693 4238 1132
Size: 97 1464 1545 2229
Fragments arranged by size:
2229 1545 1464 97
PasI CC'CwG_GG
Cuts at: 1348 2614 1348
Size: 1266 4069
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2479 1108
Size: 1371 3964
PfoI T'CCnGG_A
Cuts at: 2553 2553
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PspXI vC'TCGA_Gb
Cuts at: 2510 2510
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2687 830
Size: 1857 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 5335
SacII CC_GC'GG
Cuts at: 2025 2587 2025
Size: 562 4773
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2683 3818 4499 4690 826
Size: 219 1638 1135 681 191 1471
Fragments arranged by size:
1638 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 5335
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2662 1872
Size: 790 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 3439 4340 4602 820
Size: 197 926 567 929 901 262 1553
Fragments arranged by size:
1553 929 926 901 567 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2774 513
Size: 552 1709 3074
Fragments arranged by size:
3074 1709 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3362 3379 3532 3717 4162 325
Size: 1255 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5295 5327 5295
Size: 32 5303
Tth111I GACn'n_nGTC
Cuts at: 1316 2537 1316
Size: 1221 4114
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2380 2380
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 2510 1943
Size: 567 4768
XmaI C'CCGG_G
Cuts at: 1870 2660 1870
Size: 790 4545
XmnI GAAnn'nnTTC
Cuts at: 2354 3464 2354
Size: 1110 4225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2097 3143 276
Size: 53 83 186 936 563 1046 2468
Fragments arranged by size:
2468 1046 936 563 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PstI PvuI RsrII SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PsrI PvuII SacI SanDI
SapI SexAI SfiI SrfI SwaI