Restriction map for
pFN21AE3912
(Circular) (Six-base) MAPSORT of: pFN21AE3912.seq Check: 2654 from: 1 to: 5953
pFN21AE3912 5953 bp
With 182 enzymes: SgfI *
March 28, 2012 11:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2562 3192 3296 1937
Size: 625 630 104 4594
Fragments arranged by size:
4594 630 625 104
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4016 5400 5668 1632
Size: 2384 1384 268 1917
Fragments arranged by size:
2384 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 2796 4852 5829 1203
Size: 1593 2056 977 1327
Fragments arranged by size:
2056 1593 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AlwNI CAG_nnn'CTG
Cuts at: 2512 2531 2724 3074 3696 5268 2512
Size: 19 193 350 622 1572 3197
Fragments arranged by size:
3197 1572 622 350 193 19
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 3262 3488 3640 5749 1493
Size: 1769 226 152 2109 1697
Fragments arranged by size:
2109 1769 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2423 3206 3278 1870
Size: 73 480 783 72 4545
Fragments arranged by size:
4545 783 480 73 72
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 3274 4729 618
Size: 325 217 627 417 1070 1455 1842
Fragments arranged by size:
1842 1455 1070 627 417 325 217
BanII G_rGCy'C
Cuts at: 1391 3207 3272 1391
Size: 1816 65 4072
Fragments arranged by size:
4072 1816 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2372 928
Size: 971 473 4509
Fragments arranged by size:
4509 971 473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2249 2283 4144 4178 1903
Size: 34 312 34 1861 34 3678
Fragments arranged by size:
3678 1861 312 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BfrBI ATG'CAT
Cuts at: 2041 2041
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2696 4564 136
Size: 107 122 71 631 605 1024 1868 1525
Fragments arranged by size:
1868 1525 1024 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2646 5948 2646
Size: 3302 2651
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 2207 2226 2519 4014 5170 1790
Size: 417 19 293 1495 1156 2573
Fragments arranged by size:
2573 1495 1156 417 293 19
BmgBI CAC'GTC
Cuts at: 1318 2476 1318
Size: 1158 4795
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3068 1056
Size: 2012 3941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2652 2767 2652
Size: 115 5838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2762 3252 4613 1239
Size: 972 551 490 1361 2579
Fragments arranged by size:
2579 1361 972 551 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3087 4078 4943 5241 3087
Size: 991 865 298 3799
Fragments arranged by size:
3799 991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2328 4616 882
Size: 617 829 2288 2219
Fragments arranged by size:
2288 2219 829 617
BsaAI yAC'GTr
Cuts at: 493 1204 2799 3744 493
Size: 711 1595 945 2702
Fragments arranged by size:
2702 1595 945 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3760 4142 4777 275
Size: 53 83 186 936 2227 382 635 1451
Fragments arranged by size:
2227 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 2528 2558 1178
Size: 30 165 30 781 30 314 30 4573
Fragments arranged by size:
4573 781 314 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2779 1186
Size: 1593 4360
BseYI C'CCAG_C
Cuts at: 2230 2370 2412 2417 2531 5156 5652 2230
Size: 140 42 5 114 2625 496 2531
Fragments arranged by size:
2625 2531 496 140 114 42 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2176 2552 2737 1163
Size: 573 440 376 185 4379
Fragments arranged by size:
4379 573 440 376 185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3403 4164 5192 1540
Size: 275 186 1402 761 1028 2301
Fragments arranged by size:
2301 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3207 4014 4099 5170 2012
Size: 1195 807 85 1071 2795
Fragments arranged by size:
2795 1195 1071 807 85
BsmI GAATG_Cn'
Cuts at: 2437 2879 3464 3557 2437
Size: 442 585 93 4833
Fragments arranged by size:
4833 585 442 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2207 2226 2519 3207 3272 4014
Size: 399 222 195 19 293 688 65 742
Cuts at: 4014 4099 5170 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 688 399 293 222 195
85 65 19
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2019 2574 3020 3308 844
Size: 1175 555 446 288 3489
Fragments arranged by size:
3489 1175 555 446 288
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 1394
Size: 429 2017 3507
Fragments arranged by size:
3507 2017 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2541 3165 4597 4784 1700
Size: 110 731 624 1432 187 2869
Fragments arranged by size:
2869 1432 731 624 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 2170 2669 4013 5025 2170
Size: 499 1344 1012 3098
Fragments arranged by size:
3098 1344 1012 499
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 2724 794
Size: 1811 119 4023
Fragments arranged by size:
4023 1811 119
BstBI TT'CG_AA
Cuts at: 2664 4757 2664
Size: 2093 3860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2646 3283 3650 4035 4052 5504 5590 5602
Size: 674 637 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 674 637 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 4258
Fragments arranged by size:
4258 1143 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2828 3469 4263 4283 2265
Size: 563 641 794 20 3935
Fragments arranged by size:
3935 794 641 563 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DraIII CAC_nnn'GTG
Cuts at: 2221 2221
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918
Size: 54 1425 50 275 18 1570 889 1629
Cuts at: 5918 8
Size: 43
Fragments arranged by size:
1629 1570 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3221 3883 1353
Size: 1868 662 3423
Fragments arranged by size:
3423 1868 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3205 3205
Size: 5953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2762 3252 4016 4613 5400 5668
Size: 393 579 551 490 764 597 787 268
Cuts at: 5668 1239
Size: 1524
Fragments arranged by size:
1524 787 764 597 579 551 490 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2744 2744
Size: 5953
EcoO109I rG'GnC_Cy
Cuts at: 2175 3009 3211 2175
Size: 834 202 4917
Fragments arranged by size:
4917 834 202
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
EcoRV GAT'ATC
Cuts at: 2886 2886
Size: 5953
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 5100 1678
Size: 3422 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4594 4626 4668 4700 1373
Size: 32 135 32 3022 32 42 32 2626
Fragments arranged by size:
3022 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 3193 3297 3543 669
Size: 988 281 720 535 104 246 3079
Fragments arranged by size:
3079 988 720 535 281 246 104
HindIII A'AGCT_T
Cuts at: 748 2952 748
Size: 2204 3749
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 2785 3552 2785
Size: 767 5186
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5067 5251 1305
Size: 3762 184 2007
Fragments arranged by size:
3762 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4131
Fragments arranged by size:
4131 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 3911 4270 4429 518
Size: 648 1566 1179 359 159 2042
Fragments arranged by size:
2042 1566 1179 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2189 2400 2512 2528 3369 4046 5194 5439
Size: 583 211 112 16 841 677 1148 245
Cuts at: 5439 1606
Size: 2120
Fragments arranged by size:
2120 1148 841 677 583 245 211 112
16
NaeI GCC'GGC
Cuts at: 3167 3167
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2208 513
Size: 552 1143 4258
Fragments arranged by size:
4258 1143 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 3165 3165
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 3064 1052
Size: 2012 3941
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NsiI A_TGCA'T
Cuts at: 2043 2043
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2656 3173 3311 4856 1132
Size: 97 1427 517 138 1545 2229
Fragments arranged by size:
2229 1545 1427 517 138 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PfoI T'CCnGG_A
Cuts at: 2739 2739
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3009 3211 3009
Size: 202 5751
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PstI C_TGCA'G
Cuts at: 830 2188 2405 3305 830
Size: 1358 217 900 3478
Fragments arranged by size:
3478 1358 900 217
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2189 2400 2512 2528 2189
Size: 211 112 16 5614
Fragments arranged by size:
5614 211 112 16
SacI G_AGCT'C
Cuts at: 3207 3207
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3191 3295 1936
Size: 1255 104 4594
Fragments arranged by size:
4594 1255 104
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 2120 4201 5913 2120
Size: 2081 1712 2160
Fragments arranged by size:
2160 2081 1712
SfcI C'TryA_G
Cuts at: 826 1045 2184 2401 2494 2590 3301 4436 5117
Size: 219 1139 217 93 96 711 1135 681
Cuts at: 5117 5308 826
Size: 191 1471
Fragments arranged by size:
1471 1139 1135 711 681 219 217 191
96 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3102 4057 4958 5220 820
Size: 197 926 1159 955 901 262 1553
Fragments arranged by size:
1553 1159 955 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 2684 2750 1517
Size: 1167 66 4720
Fragments arranged by size:
4720 1167 66
StyI C'CwwG_G
Cuts at: 513 1065 2208 3392 513
Size: 552 1143 1184 3074
Fragments arranged by size:
3074 1184 1143 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2352 2625 2905 3980 3997 4150 4335
Size: 1255 772 273 280 1075 17 153 185
Cuts at: 4335 4780 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1075 772 445 280 273 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 4199 5911
Size: 274 80 33 51 1443 141 2081 1712
Cuts at: 5911 96
Size: 138
Fragments arranged by size:
2081 1712 1443 274 141 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 2240 2272 2699 2731 5913 5945 2184
Size: 32 24 32 427 32 3182 32 2192
Fragments arranged by size:
3182 2192 427 32 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2910 2910
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I FalI
FseI FspAI KasI NarI NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI