Restriction map for
pFN21AE3911
(Circular) (Six-base) MAPSORT of: pFN21AE3911.seq Check: 6937 from: 1 to: 6835
pFN21AE3911 6835 bp
With 182 enzymes: SgfI *
March 28, 2012 11:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4645 278
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3090 4178 1937
Size: 1153 1088 4594
Fragments arranged by size:
4594 1153 1088
Acc65I G'GTAC_C
Cuts at: 4156 4156
Size: 6835
AclI AA'CG_TT
Cuts at: 1563 4962 5335 1563
Size: 3399 373 3063
Fragments arranged by size:
3399 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 3305 3497 4898 6282 6550 1632
Size: 411 1262 192 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1262 411 268 192
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6638
AflIII A'CryG_T
Cuts at: 1203 3045 3690 5734 6711 1203
Size: 1842 645 2044 977 1327
Fragments arranged by size:
2044 1842 1327 977 645
AgeI A'CCGG_T
Cuts at: 5666 5666
Size: 6835
AhdI GACnn_n'nnGTC
Cuts at: 5564 5564
Size: 6835
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2613 2807 3227 1184
Size: 1429 194 420 4792
Fragments arranged by size:
4792 1429 420 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2822 2854 2822
Size: 32 6803
AlwNI CAG_nnn'CTG
Cuts at: 3682 4578 6150 3682
Size: 896 1572 4367
Fragments arranged by size:
4367 1572 896
ApaLI G'TGCA_C
Cuts at: 3316 3863 4892 6048 3316
Size: 547 1029 1156 4103
Fragments arranged by size:
4103 1156 1029 547
ApoI r'AATT_y
Cuts at: 1493 2188 3552 4144 4370 4522 6631 1493
Size: 695 1364 592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1364 695 592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5389 160
Size: 872 4357 1606
Fragments arranged by size:
4357 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
AvaI C'yCGr_G
Cuts at: 1870 1943 2404 2423 2771 4160 1870
Size: 73 461 19 348 1389 4545
Fragments arranged by size:
4545 1389 461 348 73 19
BamHI G'GATC_C
Cuts at: 4165 4532 4165
Size: 367 6468
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3445 4156 5611 618
Size: 325 217 627 1658 711 1455 1842
Fragments arranged by size:
1842 1658 1455 711 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2307 2377 2535 3192 3909 4154 1391
Size: 916 70 158 657 717 245 4072
Fragments arranged by size:
4072 916 717 657 245 158 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2616 2711 928
Size: 971 717 95 5052
Fragments arranged by size:
5052 971 717 95
BbvCI CC'TCA_GC
Cuts at: 4083 4083
Size: 6835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5026 5060 1903
Size: 34 3089 34 3678
Fragments arranged by size:
3678 3089 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4727 5937 4727
Size: 1210 5625
BclI T'GATC_A
Cuts at: 1548 2059 3405 1548
Size: 511 1346 4978
Fragments arranged by size:
4978 1346 511
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5446 136
Size: 107 122 71 631 605 3774 1525
Fragments arranged by size:
3774 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2718 4014 6830 2718
Size: 1296 2816 2723
Fragments arranged by size:
2816 2723 1296
BlpI GC'TnA_GC
Cuts at: 3879 4252 3879
Size: 373 6462
Bme1580I G_kGCm'C
Cuts at: 1790 3320 3450 3867 4896 6052 1790
Size: 1530 130 417 1029 1156 2573
Fragments arranged by size:
2573 1530 1156 1029 417 130
BmgBI CAC'GTC
Cuts at: 1318 2974 3046 1318
Size: 1656 72 5107
Fragments arranged by size:
5107 1656 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4566 5524 442
Size: 416 53 83 399 24 3149 958 1753
Fragments arranged by size:
3149 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6835
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2724 2413
Size: 311 6524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2347 2527 3023 3029 3131 3683 3731 3923
Size: 1108 180 496 6 102 552 48 192
Cuts at: 3923 5495 1239
Size: 1572 2579
Fragments arranged by size:
2579 1572 1108 552 496 192 180 102
48 6
Bpu10I CC'TnA_GC
Cuts at: 4083 4083
Size: 6835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2115 2728 2816 3245 4031 4960 5825 6123 2115
Size: 613 88 429 786 929 865 298 2827
Fragments arranged by size:
2827 929 865 786 613 429 298 88
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 5498 882
Size: 617 504 3495 2219
Fragments arranged by size:
3495 2219 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3874 4626 493
Size: 711 2670 752 2702
Fragments arranged by size:
2702 2670 752 711
BsaBI GATnn'nnATC
Cuts at: 6829 6829
Size: 6835
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2861 3375 3752 4642
Size: 53 83 186 936 1328 514 377 890
Cuts at: 4642 5024 5659 275
Size: 382 635 1451
Fragments arranged by size:
1451 1328 936 890 635 514 382 377
186 83 53
BsaWI w'CCGG_w
Cuts at: 2778 3015 4032 5268 5666 5940 6087 6498 2778
Size: 237 1017 1236 398 274 147 411 3115
Fragments arranged by size:
3115 1236 1017 411 398 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2822 2852 1178
Size: 30 165 30 1419 30 5161
Fragments arranged by size:
5161 1419 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2441 2666 2681 2684 2687 2690 2693
Size: 949 306 225 15 3 3 3 3
Cuts at: 2693 2828 3038 3211 3671 4025 1186
Size: 135 210 173 460 354 3996
Fragments arranged by size:
3996 949 460 354 306 225 210 173
135 15 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3995 4114 6038 6534 3995
Size: 119 1924 496 4296
Fragments arranged by size:
4296 1924 496 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 3223 3953 1163
Size: 573 1179 308 730 4045
Fragments arranged by size:
4045 1179 730 573 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4285 5046 6074 1540
Size: 275 186 2284 761 1028 2301
Fragments arranged by size:
2301 2284 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3015 3192 3320 3867 3909 4896 4981 6052 3015
Size: 177 128 547 42 987 85 1071 3798
Fragments arranged by size:
3798 1071 987 547 177 128 85 42
BsmI GAATG_Cn'
Cuts at: 3411 4346 4439 3411
Size: 935 93 5807
Fragments arranged by size:
5807 935 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2377 2535 3015 3192 3320 3450
Size: 399 517 70 158 480 177 128 130
Cuts at: 3450 3867 3909 4154 4896 4981 6052 1391
Size: 417 42 245 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 480 417 399 245
177 158 130 128 85 70 42
BspEI T'CCGG_A
Cuts at: 2778 6498 2778
Size: 3720 3115
BspHI T'CATG_A
Cuts at: 4724 6454 4724
Size: 1730 5105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2892 3585 4190 844
Size: 2048 693 605 3489
Fragments arranged by size:
3489 2048 693 605
BsrBI CCG'CTC
Cuts at: 1394 1823 2914 2992 3127 3310 4722 1394
Size: 429 1091 78 135 183 1412 3507
Fragments arranged by size:
3507 1412 1091 429 183 135 78
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 2361 5330 5504 65
Size: 1183 916 197 2969 174 1396
Fragments arranged by size:
2969 1396 1183 916 197 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2434 2683 3591 5479 5666 1700
Size: 110 624 249 908 1888 187 2869
Fragments arranged by size:
2869 1888 908 624 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6835
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6835
BssSI C'ACGA_G
Cuts at: 2888 4895 5907 2888
Size: 2007 1012 3816
Fragments arranged by size:
3816 2007 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6835
BstBI TT'CG_AA
Cuts at: 5639 5639
Size: 6835
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6835
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2968 2266
Size: 702 6133
BstYI r'GATC_y
Cuts at: 1972 2718 2764 2775 2799 2925 3951 4014 4029
Size: 746 46 11 24 126 1026 63 15
Cuts at: 4029 4165 4532 4917 4934 6386 6472 6484 6830
Size: 136 367 385 17 1452 86 12 346
Cuts at: 6830 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 746 385 367 346 136
126 86 63 46 24 17 15 12
11
Bsu36I CC'TnA_GG
Cuts at: 2811 3527 2811
Size: 716 6119
BtgI C'CryG_G
Cuts at: 513 1065 2004 3635 513
Size: 552 939 1631 3713
Fragments arranged by size:
3713 1631 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6096
Fragments arranged by size:
6096 683 56
BtsI GCAGTG_nn'
Cuts at: 3318 4351 5145 5165 3318
Size: 1033 794 20 4988
Fragments arranged by size:
4988 1033 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6800
DraI TTT'AAA
Cuts at: 4139 4139
Size: 6835
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5842 809
Size: 507 4526 1802
Fragments arranged by size:
4526 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2432 2442 2631
Size: 54 1425 50 275 18 602 10 189
Cuts at: 2631 2685 3684 4282 5171 6800 8
Size: 54 999 598 889 1629 43
Fragments arranged by size:
1629 1425 999 889 602 598 275 189
54 54 50 43 18 10
EagI C'GGCC_G
Cuts at: 1537 1812 4282 1537
Size: 275 2470 4090
Fragments arranged by size:
4090 2470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2658 2960 3281 3291 3548 3566 4765 1353
Size: 1305 302 321 10 257 18 1199 3423
Fragments arranged by size:
3423 1305 1199 321 302 257 18 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5422 5796 5942 387
Size: 1064 138 3833 374 146 1280
Fragments arranged by size:
3833 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3190 3907 3190
Size: 717 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2347 2527 3023 3029 3131 3305
Size: 393 411 304 180 496 6 102 174
Cuts at: 3305 3497 3683 3731 3923 4898 5495 6282 6550
Size: 192 186 48 192 975 597 787 268
Cuts at: 6550 1239
Size: 1524
Fragments arranged by size:
1524 975 787 597 496 411 393 304
268 192 192 186 180 174 102 48
6
EcoNI CCTnn'n_nnAGG
Cuts at: 2540 2759 2540
Size: 219 6616
EcoO109I rG'GnC_Cy
Cuts at: 2470 2625 3506 2470
Size: 155 881 5799
Fragments arranged by size:
5799 881 155
EcoRI G'AATT_C
Cuts at: 4144 4522 4144
Size: 378 6457
EcoRV GAT'ATC
Cuts at: 2146 2146
Size: 6835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3559 3559
Size: 6835
FseI GG_CCGG'CC
Cuts at: 2438 2438
Size: 6835
FspI TGC'GCA
Cuts at: 5341 5341
Size: 6835
HaeII r_GCGC'y
Cuts at: 1678 5982 1678
Size: 4304 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3068 3100 4006 4038 5476
Size: 32 135 32 1496 32 906 32 1438
Cuts at: 5476 5508 5550 5582 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1496 1438 906 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4179 4425 669
Size: 988 281 2241 246 3079
Fragments arranged by size:
3079 2241 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6835
HpaI GTT'AAC
Cuts at: 4425 4425
Size: 6835
KpnI G_GTAC'C
Cuts at: 4160 4160
Size: 6835
MfeI C'AATT_G
Cuts at: 4434 4434
Size: 6835
MluI A'CGCG_T
Cuts at: 6711 6711
Size: 6835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2985 3758 5949 6133 1305
Size: 1680 773 2191 184 2007
Fragments arranged by size:
2191 2007 1680 773 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3686 10
Size: 54 1425 343 1854 3159
Fragments arranged by size:
3159 1854 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3740 4793 5152 5311 518
Size: 648 2574 1053 359 159 2042
Fragments arranged by size:
2574 2042 1053 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2262 2787 3142 3468 3637 4251 4928 6076
Size: 656 525 355 326 169 614 677 1148
Cuts at: 6076 6321 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 656 614 525 355 326
245 169
NaeI GCC'GGC
Cuts at: 2436 2685 3593 2436
Size: 249 908 5678
Fragments arranged by size:
5678 908 249
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5344
Fragments arranged by size:
5344 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6835
NgoMIV G'CCGG_C
Cuts at: 2434 2683 3591 2434
Size: 249 908 5678
Fragments arranged by size:
5678 908 249
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6835
NotI GC'GGCC_GC
Cuts at: 4282 4282
Size: 6835
NspI r_CATG'y
Cuts at: 1132 1229 3366 3745 4193 5738 1132
Size: 97 2137 379 448 1545 2229
Fragments arranged by size:
2229 2137 1545 448 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6835
PciI A'CATG_T
Cuts at: 5734 5734
Size: 6835
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2902 3682 4072 1108
Size: 1794 780 390 3871
Fragments arranged by size:
3871 1794 780 390
PfoI T'CCnGG_A
Cuts at: 2504 2967 3000 3147 3165 3518 3601 3927 2504
Size: 463 33 147 18 353 83 326 5412
Fragments arranged by size:
5412 463 353 326 147 83 33 18
PmeI GTTT'AAAC
Cuts at: 4139 4139
Size: 6835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2822 2854 4904 4936 6417 6449 2822
Size: 32 2050 32 1481 32 3208
Fragments arranged by size:
3208 2050 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2625 2625
Size: 6835
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6835
PsiI TTA'TAA
Cuts at: 4405 4405
Size: 6835
PstI C_TGCA'G
Cuts at: 830 2829 3495 4187 830
Size: 1999 666 692 3478
Fragments arranged by size:
3478 1999 692 666
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6835
PvuII CAG'CTG
Cuts at: 2262 3142 2262
Size: 880 5955
SacI G_AGCT'C
Cuts at: 3192 3909 3192
Size: 717 6118
SacII CC_GC'GG
Cuts at: 3638 3638
Size: 6835
SalI G'TCGA_C
Cuts at: 1936 4177 1936
Size: 2241 4594
SanDI GG'GwC_CC
Cuts at: 2625 2625
Size: 6835
SapI GCTCTTCn'nnn_
Cuts at: 3548 3548
Size: 6835
SbfI CC_TGCA'GG
Cuts at: 4187 4187
Size: 6835
ScaI AGT'ACT
Cuts at: 5083 6795 5083
Size: 1712 5123
SexAI A'CCwGG_T
Cuts at: 3615 3615
Size: 6835
SfcI C'TryA_G
Cuts at: 826 1045 2804 2825 3491 4183 5318 5999 6190
Size: 219 1759 21 666 692 1135 681 191
Cuts at: 6190 826
Size: 1471
Fragments arranged by size:
1759 1471 1135 692 681 666 219 191
21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6835
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6835
SmaI CCC'GGG
Cuts at: 1872 2406 4162 1872
Size: 534 1756 4545
Fragments arranged by size:
4545 1756 534
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2130 2743 2795 3260 4046 4939
Size: 197 926 187 613 52 465 786 893
Cuts at: 4939 5840 6102 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 893 786 613 465 262
197 187 52
SnaBI TAC'GTA
Cuts at: 493 4626 493
Size: 4133 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6835
SphI G_CATG'C
Cuts at: 1132 3366 4193 1132
Size: 2234 827 3774
Fragments arranged by size:
3774 2234 827
SspI AAT'ATT
Cuts at: 5 52 4759 5
Size: 47 4707 2081
Fragments arranged by size:
4707 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6835
StyI C'CwwG_G
Cuts at: 513 1065 2004 2088 2229 4274 513
Size: 552 939 84 141 2045 3074
Fragments arranged by size:
3074 2045 939 552 141 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3882 4862 4879 5032 5217 5662 325
Size: 1255 2302 980 17 153 185 445 1498
Fragments arranged by size:
2302 1498 1255 980 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5081 6793 96
Size: 274 80 33 51 1443 3104 1712 138
Fragments arranged by size:
3104 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6795 6827 6795
Size: 32 6803
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6835
XbaI T'CTAG_A
Cuts at: 4171 4171
Size: 6835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2842 2842
Size: 6835
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6835
XmaI C'CCGG_G
Cuts at: 1870 2404 4160 1870
Size: 534 1756 4545
Fragments arranged by size:
4545 1756 534
XmnI GAAnn'nnTTC
Cuts at: 3542 4964 3542
Size: 1422 5413
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4643 276
Size: 53 83 186 936 3109 2468
Fragments arranged by size:
3109 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SfiI
SfoI SrfI SwaI