Restriction map for
pFN21AE3910
(Circular) (Six-base) MAPSORT of: pFN21AE3910.seq Check: 5050 from: 1 to: 6469
pFN21AE3910 6469 bp
With 182 enzymes: SgfI *
March 28, 2012 11:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2814 3812 1937
Size: 877 998 4594
Fragments arranged by size:
4594 998 877
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3050 4532 5916 6184 1632
Size: 1418 1482 1384 268 1917
Fragments arranged by size:
1917 1482 1418 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 3113 3254 3512 1166
Size: 1947 141 258 4123
Fragments arranged by size:
4123 1947 258 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 6437
AlwNI CAG_nnn'CTG
Cuts at: 2056 2260 3518 4212 5784 2056
Size: 204 1258 694 1572 2741
Fragments arranged by size:
2741 1572 1258 694 204
ApaI G_GGCC'C
Cuts at: 2833 2833
Size: 6469
ApaLI G'TGCA_C
Cuts at: 3446 4526 5682 3446
Size: 1080 1156 4233
Fragments arranged by size:
4233 1156 1080
ApoI r'AATT_y
Cuts at: 1493 2015 3778 4004 4156 6265 1493
Size: 522 1763 226 152 2109 1697
Fragments arranged by size:
2109 1763 1697 522 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 3324 3794 1870
Size: 73 1381 470 4545
Fragments arranged by size:
4545 1381 470 73
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3005 3213 3248 3790 5245
Size: 325 217 627 1218 208 35 542 1455
Cuts at: 5245 618
Size: 1842
Fragments arranged by size:
1842 1455 1218 627 542 325 217 208
35
BanII G_rGCy'C
Cuts at: 1391 2240 2329 2439 2510 2833 2934 3788 1391
Size: 849 89 110 71 323 101 854 4072
Fragments arranged by size:
4072 854 849 323 110 101 89 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2110 2198 2592 3038 3094 3692 928
Size: 971 211 88 394 446 56 598 3705
Fragments arranged by size:
3705 971 598 446 394 211 88 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 3258 1548
Size: 1710 4759
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3094 5080 136
Size: 107 122 71 631 605 1422 1986 1525
Fragments arranged by size:
1986 1525 1422 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 2424 2597 3886 2424
Size: 173 1289 5007
Fragments arranged by size:
5007 1289 173
Bme1580I G_kGCm'C
Cuts at: 1790 2833 3450 4530 5686 1790
Size: 1043 617 1080 1156 2573
Fragments arranged by size:
2573 1156 1080 1043 617
BmgBI CAC'GTC
Cuts at: 1318 2116 3367 1318
Size: 798 1251 4420
Fragments arranged by size:
4420 1251 798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 2500 3369
Size: 416 53 83 399 24 678 405 869
Cuts at: 3369 4200 5158 442
Size: 831 958 1753
Fragments arranged by size:
1753 958 869 831 678 416 405 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2161 5129 1239
Size: 922 2968 2579
Fragments arranged by size:
2968 2579 922
Bpu10I CC'TnA_GC
Cuts at: 2099 2234 2099
Size: 135 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4594 5459 5757 4594
Size: 865 298 5306
Fragments arranged by size:
5306 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5132 882
Size: 617 3633 2219
Fragments arranged by size:
3633 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2575 4260 493
Size: 711 1371 1685 2702
Fragments arranged by size:
2702 1685 1371 711
BsaBI GATnn'nnATC
Cuts at: 3233 6463 3233
Size: 3230 3239
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4276 4658 5293 275
Size: 53 83 186 936 2743 382 635 1451
Fragments arranged by size:
2743 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2625 4902 5300 5574 5721 6132 2625
Size: 2277 398 274 147 411 2962
Fragments arranged by size:
2962 2277 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2906 2936 3679 3709 1178
Size: 30 165 30 1503 30 743 30 3938
Fragments arranged by size:
3938 1503 743 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2179 2324 3687 1186
Size: 993 145 1363 3968
Fragments arranged by size:
3968 1363 993 145
BseYI C'CCAG_C
Cuts at: 5672 6168 5672
Size: 496 5973
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5896
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 2580 2934 3450 4530 4615 5686 2439
Size: 141 354 516 1080 85 1071 3222
Fragments arranged by size:
3222 1080 1071 516 354 141 85
BsmI GAATG_Cn'
Cuts at: 2469 3980 4073 2469
Size: 1511 93 4865
Fragments arranged by size:
4865 1511 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2329 2439 2510 2580 2833 2934
Size: 399 450 89 110 71 70 253 101
Cuts at: 2934 3450 3788 4530 4615 5686 1391
Size: 516 338 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 450 399 338 253
110 101 89 85 71 70
BspEI T'CCGG_A
Cuts at: 2625 6132 2625
Size: 3507 2962
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3093 3824 844
Size: 2249 731 3489
Fragments arranged by size:
3489 2249 731
BsrBI CCG'CTC
Cuts at: 1394 1823 2794 3199 4356 1394
Size: 429 971 405 1157 3507
Fragments arranged by size:
3507 1157 971 429 405
BsrDI GCAATG_nn'
Cuts at: 65 1248 2140 2188 3153 4964 5138 65
Size: 1183 892 48 965 1811 174 1396
Fragments arranged by size:
1811 1396 1183 965 892 174 48
BsrFI r'CCGG_y
Cuts at: 1700 1810 3456 5113 5300 1700
Size: 110 1646 1657 187 2869
Fragments arranged by size:
2869 1657 1646 187 110
BsrGI T'GTAC_A
Cuts at: 96 3282 96
Size: 3186 3283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 2966 794
Size: 1727 445 4297
Fragments arranged by size:
4297 1727 445
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 3115 1184
Size: 1931 4538
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 3799 4166 4551 4568 6020 6106 6118 6464
Size: 1827 367 385 17 1452 86 12 346
Cuts at: 6464 1972
Size: 1977
Fragments arranged by size:
1977 1827 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2502 2502
Size: 6469
BtgI C'CryG_G
Cuts at: 513 1065 2844 2872 3111 3240 513
Size: 552 1779 28 239 129 3742
Fragments arranged by size:
3742 1779 552 239 129 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2114 3630 487
Size: 683 56 888 1516 3326
Fragments arranged by size:
3326 1516 888 683 56
BtsI GCAGTG_nn'
Cuts at: 3606 3985 4779 4799 3606
Size: 379 794 20 5276
Fragments arranged by size:
5276 794 379 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 3773 3773
Size: 6469
DraIII CAC_nnn'GTG
Cuts at: 2966 2966
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2114 5476 809
Size: 507 798 3362 1802
Fragments arranged by size:
3362 1802 798 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2545 2795 2860
Size: 54 1425 50 275 18 715 250 65
Cuts at: 2860 2875 3916 4805 6434 8
Size: 15 1041 889 1629 43
Fragments arranged by size:
1629 1425 1041 889 715 275 250 65
54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2420 4399 1353
Size: 1067 1979 3423
Fragments arranged by size:
3423 1979 1067
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5056 5430 5576 387
Size: 1064 138 3467 374 146 1280
Fragments arranged by size:
3467 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2932 2437
Size: 495 5974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 3050 4532 5129 5916 6184 1239
Size: 393 529 889 1482 597 787 268 1524
Fragments arranged by size:
1524 1482 889 787 597 529 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2829 2830 2829
Size: 1 6468
EcoRI G'AATT_C
Cuts at: 2015 3778 4156 2015
Size: 1763 378 4328
Fragments arranged by size:
4328 1763 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 6469
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 2597 5616 1678
Size: 919 3019 2531
Fragments arranged by size:
3019 2531 919
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3047 3079 3679 3711 5110
Size: 32 135 32 1475 32 600 32 1399
Cuts at: 5110 5142 5184 5216 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1475 1399 600 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3813 4059 669
Size: 988 281 1875 246 3079
Fragments arranged by size:
3079 1875 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 2611 4068 2611
Size: 1457 5012
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3201 3448 5583 5767 1305
Size: 1896 247 2135 184 2007
Fragments arranged by size:
2135 2007 1896 247 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2862 10
Size: 54 1425 343 1030 3617
Fragments arranged by size:
3617 1425 1030 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2133 3113 3239 3254 3512 4427 4786
Size: 648 967 980 126 15 258 915 359
Cuts at: 4786 4945 518
Size: 159 2042
Fragments arranged by size:
2042 980 967 915 648 359 258 159
126 15
MspA1I CmG'CkG
Cuts at: 1606 2198 2821 3534 3885 4562 5710 5955 1606
Size: 592 623 713 351 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 623 592 351 245
NcoI C'CATG_G
Cuts at: 513 1065 3111 3240 513
Size: 552 2046 129 3742
Fragments arranged by size:
3742 2046 552 129
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 2944 3827 5372 1132
Size: 97 1715 883 1545 2229
Fragments arranged by size:
2229 1715 1545 883 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 3112 1108
Size: 1152 852 4465
Fragments arranged by size:
4465 1152 852
PfoI T'CCnGG_A
Cuts at: 2910 3065 2910
Size: 155 6314
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PmlI CAC'GTG
Cuts at: 2575 2575
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspOMI G'GGCC_C
Cuts at: 2829 2829
Size: 6469
PstI C_TGCA'G
Cuts at: 830 2052 3483 3821 830
Size: 1222 1431 338 3478
Fragments arranged by size:
3478 1431 1222 338
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 3534 3534
Size: 6469
SacI G_AGCT'C
Cuts at: 2439 2934 2439
Size: 495 5974
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 2084 4717 6429 2084
Size: 2633 1712 2124
Fragments arranged by size:
2633 2124 1712
SfcI C'TryA_G
Cuts at: 826 1045 2048 2272 3479 3817 4952 5633 5824
Size: 219 1003 224 1207 338 1135 681 191
Cuts at: 5824 826
Size: 1471
Fragments arranged by size:
1471 1207 1135 1003 681 338 224 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3326 3796 1872
Size: 1454 470 4545
Fragments arranged by size:
4545 1454 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4573 5474 5736 820
Size: 197 926 2630 901 262 1553
Fragments arranged by size:
2630 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 3111 3161 3240 3540 3908 513
Size: 552 2046 50 79 300 368 3074
Fragments arranged by size:
3074 2046 552 368 300 79 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 4496 4513 4666 4851 5296 325
Size: 1255 1025 1891 17 153 185 445 1498
Fragments arranged by size:
1891 1498 1255 1025 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2619 3135
Size: 274 80 33 51 1443 105 537 516
Cuts at: 3135 3282 4715 6427 96
Size: 147 1433 1712 138
Fragments arranged by size:
1712 1443 1433 537 516 274 147 138
105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2906 2938 3197 3229 3628 3660 6429 6461 2906
Size: 32 259 32 399 32 2769 32 2914
Fragments arranged by size:
2914 2769 399 259 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3368 1316
Size: 2052 4417
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 2719 2885 2698
Size: 21 166 6282
Fragments arranged by size:
6282 166 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6469
XmaI C'CCGG_G
Cuts at: 1870 3324 3794 1870
Size: 1454 470 4545
Fragments arranged by size:
4545 1454 470
XmnI GAAnn'nnTTC
Cuts at: 2568 2648 2660 4598 2568
Size: 80 12 1938 4439
Fragments arranged by size:
4439 1938 80 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI