Restriction map for
pFN21AE3908
(Circular) (Six-base) MAPSORT of: pFN21AE3908.seq Check: 4585 from: 1 to: 6358
pFN21AE3908 6358 bp
With 182 enzymes: SgfI *
March 28, 2012 11:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2253 4168 278
Size: 53 83 186 936 717 1915 2468
Fragments arranged by size:
2468 1915 936 717 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2667 3701 1937
Size: 730 1034 4594
Fragments arranged by size:
4594 1034 730
Acc65I G'GTAC_C
Cuts at: 3679 3679
Size: 6358
AclI AA'CG_TT
Cuts at: 1563 4485 4858 1563
Size: 2922 373 3063
Fragments arranged by size:
3063 2922 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2139 3176 3212 3523 3632 4421 5805 6073
Size: 507 1037 36 311 109 789 1384 268
Cuts at: 6073 1632
Size: 1917
Fragments arranged by size:
1917 1384 1037 789 507 311 268 109
36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6161
AflIII A'CryG_T
Cuts at: 1203 2964 2994 3000 3069 5257 6234 1203
Size: 1761 30 6 69 2188 977 1327
Fragments arranged by size:
2188 1761 1327 977 69 30 6
AgeI A'CCGG_T
Cuts at: 5189 5189
Size: 6358
AhdI GACnn_n'nnGTC
Cuts at: 5087 5087
Size: 6358
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6358
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6358
AlwNI CAG_nnn'CTG
Cuts at: 3146 4101 5673 3146
Size: 955 1572 3831
Fragments arranged by size:
3831 1572 955
ApaLI G'TGCA_C
Cuts at: 4415 5571 4415
Size: 1156 5202
ApoI r'AATT_y
Cuts at: 1493 3164 3308 3530 3667 3893 4045 6154 1493
Size: 1671 144 222 137 226 152 2109 1697
Fragments arranged by size:
2109 1697 1671 226 222 152 144 137
AseI AT'TA_AT
Cuts at: 160 1032 4912 160
Size: 872 3880 1606
Fragments arranged by size:
3880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
AvaI C'yCGr_G
Cuts at: 1870 1943 3683 1870
Size: 73 1740 4545
Fragments arranged by size:
4545 1740 73
BamHI G'GATC_C
Cuts at: 3688 4055 3688
Size: 367 5991
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2849 3105 3679 5134 618
Size: 325 217 627 1062 256 574 1455 1842
Fragments arranged by size:
1842 1455 1062 627 574 325 256 217
BanII G_rGCy'C
Cuts at: 1391 3677 1391
Size: 2286 4072
BbeI G_GCGC'C
Cuts at: 2853 3109 2853
Size: 256 6102
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5387
BbvCI CC'TCA_GC
Cuts at: 2312 2312
Size: 6358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3056 3090 3458 3492 4549 4583 1903
Size: 34 1119 34 368 34 1057 34 3678
Fragments arranged by size:
3678 1119 1057 368 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4250 5460 4250
Size: 1210 5148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6358
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2397 4969 136
Size: 107 122 71 631 605 725 2572 1525
Fragments arranged by size:
2572 1525 725 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6353 6353
Size: 6358
BlpI GC'TnA_GC
Cuts at: 3775 3775
Size: 6358
Bme1580I G_kGCm'C
Cuts at: 1790 2430 2876 2994 4419 5575 1790
Size: 640 446 118 1425 1156 2573
Fragments arranged by size:
2573 1425 1156 640 446 118
BmgBI CAC'GTC
Cuts at: 1318 2967 3070 1318
Size: 1649 103 4606
Fragments arranged by size:
4606 1649 103
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4089 5047 442
Size: 416 53 83 399 24 2672 958 1753
Fragments arranged by size:
2672 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 5018 1239
Size: 813 2966 2579
Fragments arranged by size:
2966 2579 813
Bpu10I CC'TnA_GC
Cuts at: 2312 2312
Size: 6358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3224 4483 5348 5646 3224
Size: 1259 865 298 3936
Fragments arranged by size:
3936 1259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5021 882
Size: 617 3522 2219
Fragments arranged by size:
3522 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2995 3102 4149 493
Size: 711 1791 107 1047 2702
Fragments arranged by size:
2702 1791 1047 711 107
BsaBI GATnn'nnATC
Cuts at: 2563 6352 2563
Size: 3789 2569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2850 3106 4165
Size: 53 83 186 936 717 600 256 1059
Cuts at: 4165 4547 5182 275
Size: 382 635 1451
Fragments arranged by size:
1451 1059 936 717 635 600 382 256
186 83 53
BsaWI w'CCGG_w
Cuts at: 4791 5189 5463 5610 6021 4791
Size: 398 274 147 411 5128
Fragments arranged by size:
5128 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6133
Fragments arranged by size:
6133 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3101 3392 3485 1186
Size: 1915 291 93 4059
Fragments arranged by size:
4059 1915 291 93
BseYI C'CCAG_C
Cuts at: 2395 5561 6057 2395
Size: 3166 496 2696
Fragments arranged by size:
3166 2696 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3043 1163
Size: 573 1307 4478
Fragments arranged by size:
4478 1307 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3808 4569 5597 1540
Size: 275 186 1807 761 1028 2301
Fragments arranged by size:
2301 1807 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2261 4419 4504 5575 2261
Size: 2158 85 1071 3044
Fragments arranged by size:
3044 2158 1071 85
BsmI GAATG_Cn'
Cuts at: 3481 3869 3962 3481
Size: 388 93 5877
Fragments arranged by size:
5877 388 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 2430 2876 2994 3677 4419 4504
Size: 399 471 169 446 118 683 742 85
Cuts at: 4504 5575 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 683 471 446 399 169
118 85
BspEI T'CCGG_A
Cuts at: 6021 6021
Size: 6358
BspHI T'CATG_A
Cuts at: 4247 5977 4247
Size: 1730 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2542 3713 844
Size: 1698 1171 3489
Fragments arranged by size:
3489 1698 1171
BsrBI CCG'CTC
Cuts at: 1394 1823 4245 1394
Size: 429 2422 3507
Fragments arranged by size:
3507 2422 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2176 2407 4853 5027 65
Size: 1183 928 231 2446 174 1396
Fragments arranged by size:
2446 1396 1183 928 231 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5002 5189 1700
Size: 110 3192 187 2869
Fragments arranged by size:
3192 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2997 96
Size: 2901 3457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6358
BssSI C'ACGA_G
Cuts at: 4418 5430 4418
Size: 1012 5346
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2223 794
Size: 1429 4929
BstBI TT'CG_AA
Cuts at: 5162 5162
Size: 6358
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6358
BstYI r'GATC_y
Cuts at: 1972 2558 3688 4055 4440 4457 5909 5995 6007
Size: 586 1130 367 385 17 1452 86 12
Cuts at: 6007 6353 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1130 586 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3375 3375
Size: 6358
BtgI C'CryG_G
Cuts at: 513 1065 2004 3117 513
Size: 552 939 1113 3754
Fragments arranged by size:
3754 1113 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3466 487
Size: 683 56 2240 3379
Fragments arranged by size:
3379 2240 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 3180 3874 4668 4688 2167
Size: 1013 694 794 20 3837
Fragments arranged by size:
3837 1013 794 694 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6358
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6323
DraI TTT'AAA
Cuts at: 2289 3652 3662 2289
Size: 1363 10 4985
Fragments arranged by size:
4985 1363 10
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5365 809
Size: 507 4049 1802
Fragments arranged by size:
4049 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2096 2973 3805
Size: 54 1425 50 275 18 266 877 832
Cuts at: 3805 4694 6323 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 877 832 275 266 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3805 1537
Size: 275 1993 4090
Fragments arranged by size:
4090 1993 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2587 3152 4288 1353
Size: 1234 565 1136 3423
Fragments arranged by size:
3423 1234 1136 565
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4945 5319 5465 387
Size: 1064 138 3356 374 146 1280
Fragments arranged by size:
3356 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2139 3176 3212 3523 3632 4421
Size: 393 420 87 1037 36 311 109 789
Cuts at: 4421 5018 5805 6073 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1037 789 787 597 420 393 311
268 109 87 36
EcoRI G'AATT_C
Cuts at: 3530 3667 4045 3530
Size: 137 378 5843
Fragments arranged by size:
5843 378 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2033 2033
Size: 6358
FspI TGC'GCA
Cuts at: 4864 4864
Size: 6358
HaeII r_GCGC'y
Cuts at: 1678 2853 3109 5505 1678
Size: 1175 256 2396 2531
Fragments arranged by size:
2531 2396 1175 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 4999 5031 5073
Size: 32 135 32 826 32 2569 32 42
Cuts at: 5073 5105 1373
Size: 32 2626
Fragments arranged by size:
2626 2569 826 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3702 3948 669
Size: 988 281 1764 246 3079
Fragments arranged by size:
3079 1764 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6358
HpaI GTT'AAC
Cuts at: 3948 3948
Size: 6358
KasI G'GCGC_C
Cuts at: 2849 3105 2849
Size: 256 6102
KpnI G_GTAC'C
Cuts at: 3683 3683
Size: 6358
MfeI C'AATT_G
Cuts at: 3957 3957
Size: 6358
MluI A'CGCG_T
Cuts at: 6234 6234
Size: 6358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3622 5472 5656 1305
Size: 2317 1850 184 2007
Fragments arranged by size:
2317 2007 1850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2098 10
Size: 54 1425 343 266 4270
Fragments arranged by size:
4270 1425 343 266 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4316 4675 4834 518
Size: 648 3150 359 159 2042
Fragments arranged by size:
3150 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2395 3774 4451 5599 5844 1606
Size: 789 1379 677 1148 245 2120
Fragments arranged by size:
2120 1379 1148 789 677 245
NarI GG'CG_CC
Cuts at: 2850 3106 2850
Size: 256 6102
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4867
Fragments arranged by size:
4867 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6358
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6358
NotI GC'GGCC_GC
Cuts at: 3805 3805
Size: 6358
NspI r_CATG'y
Cuts at: 1132 1229 2640 3004 3716 5261 1132
Size: 97 1411 364 712 1545 2229
Fragments arranged by size:
2229 1545 1411 712 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6358
PciI A'CATG_T
Cuts at: 3000 5257 3000
Size: 2257 4101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2497 1108
Size: 1389 4969
PfoI T'CCnGG_A
Cuts at: 3336 3336
Size: 6358
PmeI GTTT'AAAC
Cuts at: 3662 3662
Size: 6358
PmlI CAC'GTG
Cuts at: 2995 3102 2995
Size: 107 6251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4427 4459 5940 5972 4427
Size: 32 1481 32 4813
Fragments arranged by size:
4813 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6358
PsiI TTA'TAA
Cuts at: 2538 3928 2538
Size: 1390 4968
PstI C_TGCA'G
Cuts at: 830 3187 3710 830
Size: 2357 523 3478
Fragments arranged by size:
3478 2357 523
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6358
PvuII CAG'CTG
Cuts at: 2395 2395
Size: 6358
SalI G'TCGA_C
Cuts at: 1936 3700 1936
Size: 1764 4594
SbfI CC_TGCA'GG
Cuts at: 3710 3710
Size: 6358
ScaI AGT'ACT
Cuts at: 4606 6318 4606
Size: 1712 4646
SfcI C'TryA_G
Cuts at: 826 1045 2129 2187 3183 3564 3706 4841 5522
Size: 219 1084 58 996 381 142 1135 681
Cuts at: 5522 5713 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1084 996 681 381 219 191
142 58
SfoI GGC'GCC
Cuts at: 2851 3107 2851
Size: 256 6102
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6358
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6358
SmaI CCC'GGG
Cuts at: 1872 3685 1872
Size: 1813 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3239 4462 5363 5625 820
Size: 197 926 1296 1223 901 262 1553
Fragments arranged by size:
1553 1296 1223 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4149 493
Size: 3656 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6358
SphI G_CATG'C
Cuts at: 1132 3716 1132
Size: 2584 3774
SspI AAT'ATT
Cuts at: 5 52 4282 5
Size: 47 4230 2081
Fragments arranged by size:
4230 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6358
StyI C'CwwG_G
Cuts at: 513 1065 2004 3797 513
Size: 552 939 1793 3074
Fragments arranged by size:
3074 1793 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 4385 4402 4555 4740 5185 325
Size: 1255 1482 1323 17 153 185 445 1498
Fragments arranged by size:
1498 1482 1323 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2050 2997 3213
Size: 274 80 33 51 1443 73 947 216
Cuts at: 3213 4604 6316 96
Size: 1391 1712 138
Fragments arranged by size:
1712 1443 1391 947 274 216 138 80
73 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 3216 3248 6318 6350 2322
Size: 32 862 32 3070 32 2330
Fragments arranged by size:
3070 2330 862 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6358
XbaI T'CTAG_A
Cuts at: 3694 3694
Size: 6358
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2399 2399
Size: 6358
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6358
XmaI C'CCGG_G
Cuts at: 1870 3683 1870
Size: 1813 4545
XmnI GAAnn'nnTTC
Cuts at: 2016 4487 2016
Size: 2471 3887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2251 4166 276
Size: 53 83 186 936 717 1915 2468
Fragments arranged by size:
2468 1915 936 717 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI