Restriction map for
pFN21AE3907
(Circular) (Six-base) MAPSORT of: pFN21AE3907.seq Check: 4913 from: 1 to: 5968
pFN21AE3907 5968 bp
With 182 enzymes: SgfI *
March 28, 2012 11:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2286 3311 1937
Size: 349 1025 4594
Fragments arranged by size:
4594 1025 349
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2584 2669 4031 5415 5683 1632
Size: 893 59 85 1362 1384 268 1917
Fragments arranged by size:
1917 1384 1362 893 268 85 59
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5968
AlwNI CAG_nnn'CTG
Cuts at: 2203 2311 2935 3711 5283 2203
Size: 108 624 776 1572 2888
Fragments arranged by size:
2888 1572 776 624 108
ApaI G_GGCC'C
Cuts at: 2548 2548
Size: 5968
ApaLI G'TGCA_C
Cuts at: 2499 4025 5181 2499
Size: 1526 1156 3286
Fragments arranged by size:
3286 1526 1156
ApoI r'AATT_y
Cuts at: 1493 3277 3503 3655 5764 1493
Size: 1784 226 152 2109 1697
Fragments arranged by size:
2109 1784 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 3179 3293 1870
Size: 73 906 330 114 4545
Fragments arranged by size:
4545 906 330 114 73
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 3289 4744 618
Size: 325 217 627 363 1139 1455 1842
Fragments arranged by size:
1842 1455 1139 627 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2548 2841 3287 1391
Size: 1157 293 446 4072
Fragments arranged by size:
4072 1157 446 293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4997
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3170 3204 4159 4193 1903
Size: 34 1233 34 955 34 3678
Fragments arranged by size:
3678 1233 955 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2437 3860 5070 2437
Size: 1423 1210 3335
Fragments arranged by size:
3335 1423 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 3385 3385
Size: 5968
Bme1580I G_kGCm'C
Cuts at: 1790 2503 2548 2880 2968 4029 5185 1790
Size: 713 45 332 88 1061 1156 2573
Fragments arranged by size:
2573 1156 1061 713 332 88 45
BmgBI CAC'GTC
Cuts at: 1318 2022 3103 3265 1318
Size: 704 1081 162 4021
Fragments arranged by size:
4021 1081 704 162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3699 4657 442
Size: 416 53 83 399 24 2282 958 1753
Fragments arranged by size:
2282 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2249 3001 4628 1239
Size: 959 51 752 1627 2579
Fragments arranged by size:
2579 1627 959 752 51
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2441 4093 4958 5256 2441
Size: 1652 865 298 3153
Fragments arranged by size:
3153 1652 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3021 4631 882
Size: 617 1522 1610 2219
Fragments arranged by size:
2219 1610 1522 617
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3775 4157 4792 275
Size: 53 83 186 936 2242 382 635 1451
Fragments arranged by size:
2242 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2681 4401 4799 5073 5220 5631 2681
Size: 1720 398 274 147 411 3018
Fragments arranged by size:
3018 1720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2558 2588 2852
Size: 30 165 30 708 30 417 30 264
Cuts at: 2852 2882 3116 3146 1178
Size: 30 234 30 4000
Fragments arranged by size:
4000 708 417 264 234 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2246 2783 2860 2932 1186
Size: 892 168 537 77 72 4222
Fragments arranged by size:
4222 892 537 168 77 72
BseYI C'CCAG_C
Cuts at: 5171 5667 5171
Size: 496 5472
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 1163
Size: 573 831 4564
Fragments arranged by size:
4564 831 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2097 2366 3418 4179 5207 1540
Size: 275 186 96 269 1052 761 1028 2301
Fragments arranged by size:
2301 1052 1028 761 275 269 186 96
BsiHKAI G_wGCw'C
Cuts at: 2115 2238 2503 2841 4029 4114 5185 2115
Size: 123 265 338 1188 85 1071 2898
Fragments arranged by size:
2898 1188 1071 338 265 123 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
BsmBI CGTCTCn'nnnn_
Cuts at: 3158 3251 3158
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2115 2238 2503 2548 2841 2880 2968
Size: 399 325 123 265 45 293 39 88
Cuts at: 2968 3287 4029 4114 5185 1391
Size: 319 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 319 293 265
123 88 85 45 39
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3039 3323 844
Size: 2195 284 3489
Fragments arranged by size:
3489 2195 284
BsrBI CCG'CTC
Cuts at: 1394 1823 3855 1394
Size: 429 2032 3507
Fragments arranged by size:
3507 2032 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3232 4463 4637 65
Size: 1183 1984 1231 174 1396
Fragments arranged by size:
1984 1396 1231 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2365 2706 4612 4799 1700
Size: 110 555 341 1906 187 2869
Fragments arranged by size:
2869 1906 555 341 187 110
BsrGI T'GTAC_A
Cuts at: 96 2574 96
Size: 2478 3490
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2935 794
Size: 2141 3827
BstBI TT'CG_AA
Cuts at: 2692 4772 2692
Size: 2080 3888
BstEII G'GTnAC_C
Cuts at: 1184 2419 2893 1184
Size: 1235 474 4259
Fragments arranged by size:
4259 1235 474
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 3211 2137
Size: 1074 4894
BstYI r'GATC_y
Cuts at: 1972 2013 3298 3665 4050 4067 5519 5605 5617
Size: 41 1285 367 385 17 1452 86 12
Cuts at: 5617 5963 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1285 385 367 346 86 41
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3216 513
Size: 552 2151 3265
Fragments arranged by size:
3265 2151 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2248 487
Size: 683 56 1022 4207
Fragments arranged by size:
4207 1022 683 56
BtsI GCAGTG_nn'
Cuts at: 2537 3086 3484 4278 4298 2537
Size: 549 398 794 20 4207
Fragments arranged by size:
4207 794 549 398 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DraIII CAC_nnn'GTG
Cuts at: 2993 2993
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2363 2708 3213
Size: 54 1425 50 275 18 533 345 505
Cuts at: 3213 3415 4304 5933 8
Size: 202 889 1629 43
Fragments arranged by size:
1629 1425 889 533 505 345 275 202
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2363 3415 1537
Size: 275 551 1052 4090
Fragments arranged by size:
4090 1052 551 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2129 2756 3898 1353
Size: 776 627 1142 3423
Fragments arranged by size:
3423 1142 776 627
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2620 3113 4555 4929 5075 387
Size: 1064 138 1031 493 1442 374 146 1280
Fragments arranged by size:
1442 1280 1064 1031 493 374 146 138
EcoICRI GAG'CTC
Cuts at: 2839 2839
Size: 5968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2249 2525 2584 2669 3001 4031
Size: 393 566 51 276 59 85 332 1030
Cuts at: 4031 4628 5415 5683 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1030 787 597 566 393 332 276
268 85 59 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 3035 2060
Size: 975 4993
EcoO109I rG'GnC_Cy
Cuts at: 2173 2341 2377 2544 3010 2173
Size: 168 36 167 466 5131
Fragments arranged by size:
5131 466 168 167 36
EcoRI G'AATT_C
Cuts at: 3277 3655 3277
Size: 378 5590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 5968
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 2984 5115 1678
Size: 1306 2131 2531
Fragments arranged by size:
2531 2131 1306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3229 3261 4609 4641 4683
Size: 32 135 32 1657 32 1348 32 42
Cuts at: 4683 4715 1373
Size: 32 2626
Fragments arranged by size:
2626 1657 1348 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 2154 2301 5082 5266 1305
Size: 699 150 147 2781 184 2007
Fragments arranged by size:
2781 2007 699 184 150 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4146
Fragments arranged by size:
4146 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 3926 4285 4444 518
Size: 648 891 1869 359 159 2042
Fragments arranged by size:
2042 1869 891 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2416 2593 3384 4061 5209 5454 1606
Size: 579 231 177 791 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 677 579 245 231 177
NaeI GCC'GGC
Cuts at: 2367 2708 2367
Size: 341 5627
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5416
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NgoMIV G'CCGG_C
Cuts at: 2365 2706 2365
Size: 341 5627
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NruI TCG'CGA
Cuts at: 3065 3065
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 3326 4871 1132
Size: 97 2097 1545 2229
Fragments arranged by size:
2229 2097 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2464 1348
Size: 1116 4852
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2203 2554 1108
Size: 1095 351 4522
Fragments arranged by size:
4522 1095 351
PfoI T'CCnGG_A
Cuts at: 2136 2784 3153 2136
Size: 648 369 4951
Fragments arranged by size:
4951 648 369
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2173
Size: 5968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PspOMI G'GGCC_C
Cuts at: 2544 2544
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2076 2307 3093 3120 3320 830
Size: 1246 231 786 27 200 3478
Fragments arranged by size:
3478 1246 786 231 200 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2593 2593
Size: 5968
SacI G_AGCT'C
Cuts at: 2841 2841
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2756 2756
Size: 5968
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 2249 4216 5928 2249
Size: 1967 1712 2289
Fragments arranged by size:
2289 1967 1712
SfcI C'TryA_G
Cuts at: 826 1045 2072 2303 3089 3116 3316 4451 5132
Size: 219 1027 231 786 27 200 1135 681
Cuts at: 5132 5323 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1027 786 681 231 219 200
191 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 2851 3295 1872
Size: 979 444 4545
Fragments arranged by size:
4545 979 444
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2456 4072 4973 5235 820
Size: 197 926 513 1616 901 262 1553
Fragments arranged by size:
1616 1553 926 901 513 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 3056 1517
Size: 1539 4429
StyI C'CwwG_G
Cuts at: 513 1065 2079 3051 3407 513
Size: 552 1014 972 356 3074
Fragments arranged by size:
3074 1014 972 552 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 3120 3995 4012 4165 4350 4795
Size: 1255 558 982 875 17 153 185 445
Cuts at: 4795 325
Size: 1498
Fragments arranged by size:
1498 1255 982 875 558 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2574 4214
Size: 274 80 33 51 1443 270 327 1640
Cuts at: 4214 5926 96
Size: 1712 138
Fragments arranged by size:
1712 1640 1443 327 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 3116 3148 5928 5960 2111
Size: 32 973 32 2780 32 2119
Fragments arranged by size:
2780 2119 973 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3080 3104 1316
Size: 1764 24 4180
Fragments arranged by size:
4180 1764 24
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2518 2518
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 2849 3293 1870
Size: 979 444 4545
Fragments arranged by size:
4545 979 444
XmnI GAAnn'nnTTC
Cuts at: 2249 4097 2249
Size: 1848 4120
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NarI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI