Restriction map for
pFN21AE3890
(Circular) (Six-base) MAPSORT of: pFN21AE3890.seq Check: 1912 from: 1 to: 6622
pFN21AE3890 6622 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3381 3965 1937
Size: 1444 584 4594
Fragments arranged by size:
4594 1444 584
Acc65I G'GTAC_C
Cuts at: 3943 3943
Size: 6622
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3123 3614 4685 6069 6337 1632
Size: 1491 491 1071 1384 268 1917
Fragments arranged by size:
1917 1491 1384 1071 491 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 5521 6498 1203
Size: 4318 977 1327
Fragments arranged by size:
4318 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 5351 5351
Size: 6622
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 2549 2752 4365 5937 2549
Size: 203 1613 1572 3234
Fragments arranged by size:
3234 1613 1572 203
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 2189 2286 2482 2634 3311 3430 3745 3931
Size: 696 97 196 152 677 119 315 186
Cuts at: 3931 4157 4309 6418 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 677 315 226 196 186
152 152 119 97
AseI AT'TA_AT
Cuts at: 160 1032 2415 3282 5176 160
Size: 872 1383 867 1894 1606
Fragments arranged by size:
1894 1606 1383 872 867
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 3947 1870
Size: 73 2004 4545
Fragments arranged by size:
4545 2004 73
BamHI G'GATC_C
Cuts at: 3952 4319 3952
Size: 367 6255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3943 5398 618
Size: 325 217 627 2156 1455 1842
Fragments arranged by size:
2156 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3769 3941 1391
Size: 2378 172 4072
Fragments arranged by size:
4072 2378 172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2330 3585 3866 3909 928
Size: 971 431 1255 281 43 3641
Fragments arranged by size:
3641 1255 971 431 281 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4813 4847 1903
Size: 34 2876 34 3678
Fragments arranged by size:
3678 2876 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4514 5724 4514
Size: 1210 5412
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5233 136
Size: 107 122 71 631 605 3561 1525
Fragments arranged by size:
3561 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3212 6617 3212
Size: 3405 3217
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 4683 5839 1790
Size: 2893 1156 2573
Fragments arranged by size:
2893 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2762 4353 5311
Size: 416 53 83 399 24 1345 1591 958
Cuts at: 5311 442
Size: 1753
Fragments arranged by size:
1753 1591 1345 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 2405 2934 3030 5282 1239
Size: 1062 104 529 96 2252 2579
Fragments arranged by size:
2579 2252 1062 529 104 96
Bpu10I CC'TnA_GC
Cuts at: 3039 3039
Size: 6622
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4747 5612 5910 4747
Size: 865 298 5459
Fragments arranged by size:
5459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5285 882
Size: 617 3786 2219
Fragments arranged by size:
3786 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4413 493
Size: 711 3209 2702
Fragments arranged by size:
3209 2702 711
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4429 4811 5446 275
Size: 53 83 186 936 2896 382 635 1451
Fragments arranged by size:
2896 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5055 5453 5727 5874 6285 5055
Size: 398 274 147 411 5392
Fragments arranged by size:
5392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 5674
Fragments arranged by size:
5674 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6622
BseYI C'CCAG_C
Cuts at: 2558 5825 6321 2558
Size: 3267 496 2859
Fragments arranged by size:
3267 2859 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6049
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4072 4833 5861 1540
Size: 275 186 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4683 4768 5839 4683
Size: 85 1071 5466
Fragments arranged by size:
5466 1071 85
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
BsmBI CGTCTCn'nnnn_
Cuts at: 2177 2177
Size: 6622
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3769 3941 4683 4768 5839 1391
Size: 399 1979 172 742 85 1071 2174
Fragments arranged by size:
2174 1979 1071 742 399 172 85
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 2524 4511 6241 2524
Size: 1987 1730 2905
Fragments arranged by size:
2905 1987 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3977 844
Size: 3133 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4509 1394
Size: 429 2686 3507
Fragments arranged by size:
3507 2686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5117 5291 65
Size: 1183 3869 174 1396
Fragments arranged by size:
3869 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5266 5453 1700
Size: 110 3456 187 2869
Fragments arranged by size:
3456 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2041 2248 96
Size: 1945 207 4470
Fragments arranged by size:
4470 1945 207
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 4682 5694 4682
Size: 1012 5610
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6622
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6622
BstYI r'GATC_y
Cuts at: 1972 3212 3952 4319 4704 4721 6173 6259 6271
Size: 1240 740 367 385 17 1452 86 12
Cuts at: 6271 6617 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1240 740 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5131
Fragments arranged by size:
5131 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5883
Fragments arranged by size:
5883 683 56
BtsI GCAGTG_nn'
Cuts at: 3471 4138 4932 4952 3471
Size: 667 794 20 5141
Fragments arranged by size:
5141 794 667 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6587
DraI TTT'AAA
Cuts at: 2665 2875 3096 3926 2665
Size: 210 221 830 5361
Fragments arranged by size:
5361 830 221 210
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4069 4958 6587
Size: 54 1425 50 275 18 2239 889 1629
Cuts at: 6587 8
Size: 43
Fragments arranged by size:
2239 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2134 2415 2709 3849 4552 1353
Size: 781 281 294 1140 703 3423
Fragments arranged by size:
3423 1140 781 703 294 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5209 5583 5729 387
Size: 1064 138 3620 374 146 1280
Fragments arranged by size:
3620 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2405 2934 3030 3123 3614 4685
Size: 393 669 104 529 96 93 491 1071
Cuts at: 4685 5282 6069 6337 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1071 787 669 597 529 491 393
268 104 96 93
EcoO109I rG'GnC_Cy
Cuts at: 2756 3405 2756
Size: 649 5973
EcoRI G'AATT_C
Cuts at: 2189 2286 3931 4309 2189
Size: 97 1645 378 4502
Fragments arranged by size:
4502 1645 378 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2882 2882
Size: 6622
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 5769 1678
Size: 4091 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 3104 3136 3314
Size: 32 135 32 492 32 1008 32 178
Cuts at: 3314 3346 3859 3891 5263 5295 5337 5369 1373
Size: 32 513 32 1372 32 42 32 2626
Fragments arranged by size:
2626 1372 1008 513 492 178 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2011 3966 4212 669
Size: 988 281 73 1955 246 3079
Fragments arranged by size:
3079 1955 988 281 246 73
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6622
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KpnI G_GTAC'C
Cuts at: 3947 3947
Size: 6622
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2050 3560 5736 5920 1305
Size: 745 1510 2176 184 2007
Fragments arranged by size:
2176 2007 1510 745 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4800
Fragments arranged by size:
4800 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2049 2232 4580 4939 5098 518
Size: 648 883 183 2348 359 159 2042
Fragments arranged by size:
2348 2042 883 648 359 183 159
MspA1I CmG'CkG
Cuts at: 1606 2279 3358 4038 4715 5863 6108 1606
Size: 673 1079 680 677 1148 245 2120
Fragments arranged by size:
2120 1148 1079 680 677 673 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5131
Fragments arranged by size:
5131 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 3624 3980 5525 1132
Size: 97 2395 356 1545 2229
Fragments arranged by size:
2395 2229 1545 356 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6622
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2756 3405 2756
Size: 649 5973
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PstI C_TGCA'G
Cuts at: 830 3076 3974 830
Size: 2246 898 3478
Fragments arranged by size:
3478 2246 898
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 2279 3358 2279
Size: 1079 5543
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SanDI GG'GwC_CC
Cuts at: 2756 2756
Size: 6622
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 2099 2744 3072 3270 3970 5105 5786
Size: 219 1054 645 328 198 700 1135 681
Cuts at: 5786 5977 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1054 700 681 645 328 219
198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3949 1872
Size: 2077 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4726 5627 5889 820
Size: 197 926 2783 901 262 1553
Fragments arranged by size:
2783 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 2822 3086 4546 5
Size: 47 2770 264 1460 2081
Fragments arranged by size:
2770 2081 1460 264 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6622
StyI C'CwwG_G
Cuts at: 513 1065 2004 4061 513
Size: 552 939 2057 3074
Fragments arranged by size:
3074 2057 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3184 4649 4666 4819 5004 5449 325
Size: 1255 1604 1465 17 153 185 445 1498
Fragments arranged by size:
1604 1498 1465 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2041 2248 2358
Size: 274 80 33 51 1443 64 207 110
Cuts at: 2358 4868 6580 96
Size: 2510 1712 138
Fragments arranged by size:
2510 1712 1443 274 207 138 110 80
64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 6582 6614 2096
Size: 32 4454 32 2104
Fragments arranged by size:
4454 2104 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3877 3958 3877
Size: 81 6541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 3947 1870
Size: 2077 4545
XmnI GAAnn'nnTTC
Cuts at: 4751 4751
Size: 6622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI