Restriction map for
pFN21AE3889
(Circular) (Six-base) MAPSORT of: pFN21AE3889.seq Check: 3750 from: 1 to: 4840
pFN21AE3889 4840 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2650 278
Size: 53 83 186 936 1114 2468
Fragments arranged by size:
2468 1114 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2183 1937
Size: 246 4594
Acc65I G'GTAC_C
Cuts at: 2161 2161
Size: 4840
AclI AA'CG_TT
Cuts at: 1563 2967 3340 1563
Size: 1404 373 3063
Fragments arranged by size:
3063 1404 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 2094 2903 4287 4555 1632
Size: 446 16 809 1384 268 1917
Fragments arranged by size:
1917 1384 809 446 268 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4643
AflIII A'CryG_T
Cuts at: 1203 3739 4716 1203
Size: 2536 977 1327
Fragments arranged by size:
2536 1327 977
AgeI A'CCGG_T
Cuts at: 3671 3671
Size: 4840
AhdI GACnn_n'nnGTC
Cuts at: 3569 3569
Size: 4840
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4840
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4840
AlwNI CAG_nnn'CTG
Cuts at: 2583 4155 2583
Size: 1572 3268
ApaLI G'TGCA_C
Cuts at: 2897 4053 2897
Size: 1156 3684
ApoI r'AATT_y
Cuts at: 1493 2149 2375 2527 4636 1493
Size: 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 656 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3394 160
Size: 872 2362 1606
Fragments arranged by size:
2362 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4840
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 1870
Size: 73 222 4545
Fragments arranged by size:
4545 222 73
BamHI G'GATC_C
Cuts at: 2170 2537 2170
Size: 367 4473
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 3616 618
Size: 325 217 627 374 1455 1842
Fragments arranged by size:
1842 1455 627 374 325 217
BanII G_rGCy'C
Cuts at: 1391 2159 1391
Size: 768 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3031 3065 1903
Size: 34 1094 34 3678
Fragments arranged by size:
3678 1094 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2732 3942 2732
Size: 1210 3630
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3451 136
Size: 107 122 71 631 605 1779 1525
Fragments arranged by size:
1779 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4835 4835
Size: 4840
BlpI GC'TnA_GC
Cuts at: 2257 2257
Size: 4840
Bme1580I G_kGCm'C
Cuts at: 1790 2901 4057 1790
Size: 1111 1156 2573
Fragments arranged by size:
2573 1156 1111
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4840
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2571 3529 442
Size: 416 53 83 399 24 1154 958 1753
Fragments arranged by size:
1753 1154 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3500 1239
Size: 2261 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2965 3830 4128 2965
Size: 865 298 3677
Fragments arranged by size:
3677 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3503 882
Size: 617 2004 2219
Fragments arranged by size:
2219 2004 617
BsaAI yAC'GTr
Cuts at: 493 1204 2631 493
Size: 711 1427 2702
Fragments arranged by size:
2702 1427 711
BsaBI GATnn'nnATC
Cuts at: 4834 4834
Size: 4840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2647 3029 3664 275
Size: 53 83 186 936 1114 382 635 1451
Fragments arranged by size:
1451 1114 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3273 3671 3945 4092 4503 3273
Size: 398 274 147 411 3610
Fragments arranged by size:
3610 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4615
Fragments arranged by size:
4615 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4840
BseYI C'CCAG_C
Cuts at: 4043 4539 4043
Size: 496 4344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2290 3051 4079 1540
Size: 275 186 289 761 1028 2301
Fragments arranged by size:
2301 1028 761 289 275 186
BsiHKAI G_wGCw'C
Cuts at: 2901 2986 4057 2901
Size: 85 1071 3684
Fragments arranged by size:
3684 1071 85
BsmI GAATG_Cn'
Cuts at: 2351 2444 2351
Size: 93 4747
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2901 2986 4057 1391
Size: 399 369 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 85
BspEI T'CCGG_A
Cuts at: 4503 4503
Size: 4840
BspHI T'CATG_A
Cuts at: 2729 4459 2729
Size: 1730 3110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2195 844
Size: 1351 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2727 1394
Size: 429 904 3507
Fragments arranged by size:
3507 904 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3335 3509 65
Size: 1183 2087 174 1396
Fragments arranged by size:
2087 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3484 3671 1700
Size: 110 1674 187 2869
Fragments arranged by size:
2869 1674 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4840
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4840
BssSI C'ACGA_G
Cuts at: 2900 3912 2900
Size: 1012 3828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4840
BstBI TT'CG_AA
Cuts at: 3644 3644
Size: 4840
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4840
BstYI r'GATC_y
Cuts at: 1972 2170 2537 2922 2939 4391 4477 4489 4835
Size: 198 367 385 17 1452 86 12 346
Cuts at: 4835 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 198 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4288
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4101
Fragments arranged by size:
4101 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 3150 3170 2356
Size: 794 20 4026
Fragments arranged by size:
4026 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4840
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4805
DraI TTT'AAA
Cuts at: 2144 2144
Size: 4840
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3847 809
Size: 507 2531 1802
Fragments arranged by size:
2531 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2287 3176 4805
Size: 54 1425 50 275 18 457 889 1629
Cuts at: 4805 8
Size: 43
Fragments arranged by size:
1629 1425 889 457 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2287 1537
Size: 275 475 4090
Fragments arranged by size:
4090 475 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2770 1353
Size: 741 676 3423
Fragments arranged by size:
3423 741 676
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3427 3801 3947 387
Size: 1064 138 1838 374 146 1280
Fragments arranged by size:
1838 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2094 2903 3500 4287 4555 1239
Size: 393 446 16 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 597 446 393 268 16
EcoRI G'AATT_C
Cuts at: 2149 2527 2149
Size: 378 4462
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 4840
FspI TGC'GCA
Cuts at: 3346 3346
Size: 4840
HaeII r_GCGC'y
Cuts at: 1678 3987 1678
Size: 2309 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3481 3513 3555 3587 1373
Size: 32 135 32 1909 32 42 32 2626
Fragments arranged by size:
2626 1909 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2184 2430 669
Size: 988 281 246 246 3079
Fragments arranged by size:
3079 988 281 246 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4840
HpaI GTT'AAC
Cuts at: 2430 2430
Size: 4840
KpnI G_GTAC'C
Cuts at: 2165 2165
Size: 4840
MfeI C'AATT_G
Cuts at: 2439 2439
Size: 4840
MluI A'CGCG_T
Cuts at: 4716 4716
Size: 4840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 3954 4138 1305
Size: 702 1947 184 2007
Fragments arranged by size:
2007 1947 702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3018
Fragments arranged by size:
3018 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 3157 3316 518
Size: 648 1632 359 159 2042
Fragments arranged by size:
2042 1632 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2256 2933 4081 4326 1606
Size: 650 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 650 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4288
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4840
NotI GC'GGCC_GC
Cuts at: 2287 2287
Size: 4840
NspI r_CATG'y
Cuts at: 1132 1229 2198 3743 1132
Size: 97 969 1545 2229
Fragments arranged by size:
2229 1545 969 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4840
PciI A'CATG_T
Cuts at: 3739 3739
Size: 4840
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4840
PmeI GTTT'AAAC
Cuts at: 2144 2144
Size: 4840
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 4422 4454 2909
Size: 32 1481 32 3295
Fragments arranged by size:
3295 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4840
PsiI TTA'TAA
Cuts at: 2410 2410
Size: 4840
PstI C_TGCA'G
Cuts at: 830 2192 830
Size: 1362 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4840
SalI G'TCGA_C
Cuts at: 1936 2182 1936
Size: 246 4594
SbfI CC_TGCA'GG
Cuts at: 2192 2192
Size: 4840
ScaI AGT'ACT
Cuts at: 3088 4800 3088
Size: 1712 3128
SfcI C'TryA_G
Cuts at: 826 1045 2188 3323 4004 4195 826
Size: 219 1143 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4840
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4840
SmaI CCC'GGG
Cuts at: 1872 2167 1872
Size: 295 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2944 3845 4107 820
Size: 197 926 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2631 493
Size: 2138 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4840
SphI G_CATG'C
Cuts at: 1132 2198 1132
Size: 1066 3774
SspI AAT'ATT
Cuts at: 5 52 2764 5
Size: 47 2712 2081
Fragments arranged by size:
2712 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4840
StyI C'CwwG_G
Cuts at: 513 1065 2279 513
Size: 552 1214 3074
Fragments arranged by size:
3074 1214 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2867 2884 3037 3222 3667 325
Size: 1255 1287 17 153 185 445 1498
Fragments arranged by size:
1498 1287 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3086 4798 96
Size: 274 80 33 51 1443 1109 1712 138
Fragments arranged by size:
1712 1443 1109 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4800 4832 4800
Size: 32 4808
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4840
XbaI T'CTAG_A
Cuts at: 2176 2176
Size: 4840
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4840
XmaI C'CCGG_G
Cuts at: 1870 2165 1870
Size: 295 4545
XmnI GAAnn'nnTTC
Cuts at: 2969 2969
Size: 4840
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2648 276
Size: 53 83 186 936 1114 2468
Fragments arranged by size:
2468 1114 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI