Restriction map for
pFN21AE3888
(Circular) (Six-base) MAPSORT of: pFN21AE3888.seq Check: 273 from: 1 to: 5617
pFN21AE3888 5617 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2125 2938 2125
Size: 813 4804
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 3680 5064 5332 1632
Size: 972 1076 1384 268 1917
Fragments arranged by size:
1917 1384 1076 972 268
AfeI AGC'GCT
Cuts at: 2342 2342
Size: 5617
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2736 4516 5493 1203
Size: 1533 1780 977 1327
Fragments arranged by size:
1780 1533 1327 977
AgeI A'CCGG_T
Cuts at: 2553 4448 2553
Size: 1895 3722
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 2756 2822 1166
Size: 1590 66 3961
Fragments arranged by size:
3961 1590 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2437 1184
Size: 1253 4364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 5585
AlwNI CAG_nnn'CTG
Cuts at: 2888 3360 4932 2888
Size: 472 1572 3573
Fragments arranged by size:
3573 1572 472
ApaLI G'TGCA_C
Cuts at: 2493 2871 3674 4830 2493
Size: 378 803 1156 3280
Fragments arranged by size:
3280 1156 803 378
ApoI r'AATT_y
Cuts at: 1493 2375 2426 2537 2926 3152 3304 5413 1493
Size: 882 51 111 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 882 389 226 152 111 51
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 2701 2721 2903 2942 1870
Size: 73 275 483 20 182 39 4545
Fragments arranged by size:
4545 483 275 182 73 39 20
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2938 4393 618
Size: 325 217 627 338 813 1455 1842
Fragments arranged by size:
1842 1455 813 627 338 325 217
BanII G_rGCy'C
Cuts at: 1391 2076 2936 1391
Size: 685 860 4072
Fragments arranged by size:
4072 860 685
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 928
Size: 971 354 4292
Fragments arranged by size:
4292 971 354
BbvCI CC'TCA_GC
Cuts at: 2774 2774
Size: 5617
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2195 4228 136
Size: 107 122 71 631 605 523 2033 1525
Fragments arranged by size:
2033 1525 631 605 523 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 2497 2875 3678 4834 1790
Size: 707 378 803 1156 2573
Fragments arranged by size:
2573 1156 803 707 378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2174 2405 2716 2891 2174
Size: 231 311 175 4900
Fragments arranged by size:
4900 311 231 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4277 1239
Size: 3038 2579
Bpu10I CC'TnA_GC
Cuts at: 2774 2881 2774
Size: 107 5510
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2058 4280 882
Size: 617 559 2222 2219
Fragments arranged by size:
2222 2219 617 559
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2553 2659 4050 4448 4722 4869 5280 2553
Size: 106 1391 398 274 147 411 2890
Fragments arranged by size:
2890 1391 411 398 274 147 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 4537
Fragments arranged by size:
4537 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2270 2921 1186
Size: 946 138 651 3882
Fragments arranged by size:
3882 946 651 138
BseYI C'CCAG_C
Cuts at: 2075 2228 4820 5316 2075
Size: 153 2592 496 2376
Fragments arranged by size:
2592 2376 496 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2490 1163
Size: 573 388 366 4290
Fragments arranged by size:
4290 573 388 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2497 2875 3678 3763 4834 2497
Size: 378 803 85 1071 3280
Fragments arranged by size:
3280 1071 803 378 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2497 2875 2936 3678 3763 4834
Size: 399 286 421 378 61 742 85 1071
Cuts at: 4834 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 421 399 378 286 85
61
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2113 2972 844
Size: 1269 859 3489
Fragments arranged by size:
3489 1269 859
BsrBI CCG'CTC
Cuts at: 1394 1823 2308 3504 1394
Size: 429 485 1196 3507
Fragments arranged by size:
3507 1196 485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2356 2619 4112 4286 65
Size: 1183 1108 263 1493 174 1396
Fragments arranged by size:
1493 1396 1183 1108 263 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2553 4261 4448 1700
Size: 110 743 1708 187 2869
Fragments arranged by size:
2869 1708 743 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2468 794
Size: 1674 3943
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2545 2947 3314 3699 3716 5168 5254 5266
Size: 573 402 367 385 17 1452 86 12
Cuts at: 5266 5612 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 573 402 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2889 2889
Size: 5617
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2600 3133 3927 3947 2600
Size: 533 794 20 4270
Fragments arranged by size:
4270 794 533 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2128 2163 529
Size: 35 1564 35 3983
Fragments arranged by size:
3983 1564 35 35
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DraIII CAC_nnn'GTG
Cuts at: 2025 2135 2025
Size: 110 5507
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2801 3064 3953
Size: 54 1425 50 275 18 971 263 889
Cuts at: 3953 5582 8
Size: 1629 43
Fragments arranged by size:
1629 1425 971 889 275 263 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3547 1353
Size: 2194 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2604 3680 4277 5064 5332 1239
Size: 393 972 1076 597 787 268 1524
Fragments arranged by size:
1524 1076 972 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2885 2885
Size: 5617
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
EcoRV GAT'ATC
Cuts at: 2155 2155
Size: 5617
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 2296 2344 4764 1678
Size: 618 48 2420 2531
Fragments arranged by size:
2531 2420 618 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 4258 4290 4332
Size: 32 135 32 656 32 1998 32 42
Cuts at: 4332 4364 1373
Size: 32 2626
Fragments arranged by size:
2626 1998 656 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2365 2961 3207 669
Size: 988 281 226 201 596 246 3079
Fragments arranged by size:
3079 988 596 281 246 226 201
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2129 2942 2129
Size: 813 4804
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4731 4915 1305
Size: 3426 184 2007
Fragments arranged by size:
3426 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2803 10
Size: 54 1425 343 971 2824
Fragments arranged by size:
2824 1425 971 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2756 2822 3575 3934 4093 518
Size: 648 1590 66 753 359 159 2042
Fragments arranged by size:
2042 1590 753 648 359 159 66
MspA1I CmG'CkG
Cuts at: 1606 2206 2596 3033 3710 4858 5103 1606
Size: 600 390 437 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 600 437 390 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2740 2975 4520 1132
Size: 97 1511 235 1545 2229
Fragments arranged by size:
2229 1545 1511 235 97
PasI CC'CwG_GG
Cuts at: 1348 2047 1348
Size: 699 4918
PciI A'CATG_T
Cuts at: 2736 4516 2736
Size: 1780 3837
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2135 1108
Size: 1027 4590
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2969 830
Size: 2139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
PvuII CAG'CTG
Cuts at: 2206 2206
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2782 2965 4100 4781 4972 826
Size: 219 1737 183 1135 681 191 1471
Fragments arranged by size:
1737 1471 1135 681 219 191 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2723 2944 1872
Size: 851 221 4545
Fragments arranged by size:
4545 851 221
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3721 4622 4884 820
Size: 197 926 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 2635 152
Size: 2483 3134
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 2198 2798 1517
Size: 681 600 4336
Fragments arranged by size:
4336 681 600
StyI C'CwwG_G
Cuts at: 513 1065 2004 3056 513
Size: 552 939 1052 3074
Fragments arranged by size:
3074 1052 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3661 3814 3999 4444 325
Size: 1255 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5577 5609 5577
Size: 32 5585
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2721 2942 1870
Size: 851 221 4545
Fragments arranged by size:
4545 851 221
XmnI GAAnn'nnTTC
Cuts at: 2738 3746 2738
Size: 1008 4609
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI