Restriction map for
pFN21AE3887
(Circular) (Six-base) MAPSORT of: pFN21AE3887.seq Check: 9957 from: 1 to: 5731
pFN21AE3887 5731 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 2326 3858 4231 1563
Size: 763 1532 373 3063
Fragments arranged by size:
3063 1532 763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 2798 3794 5178 5446 1632
Size: 968 198 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 968 268 198
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 2914 4630 5607 1203
Size: 1711 1716 977 1327
Fragments arranged by size:
1716 1711 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 2573 2768 3040 3266 3418 5527 1493
Size: 1080 195 272 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 272 226 195 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 3056 1870
Size: 73 1113 4545
Fragments arranged by size:
4545 1113 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 3050 1391
Size: 712 947 4072
Fragments arranged by size:
4072 947 712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 928
Size: 971 564 4196
Fragments arranged by size:
4196 971 564
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2876 3623 4833 2876
Size: 747 1210 3774
Fragments arranged by size:
3774 1210 747
BclI T'GATC_A
Cuts at: 1548 2407 2545 1548
Size: 859 138 4734
Fragments arranged by size:
4734 859 138
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 3792 4948 1790
Size: 2002 1156 2573
Fragments arranged by size:
2573 2002 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2469 4391 1239
Size: 1230 1922 2579
Fragments arranged by size:
2579 1922 1230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2791 3522 493
Size: 711 1587 731 2702
Fragments arranged by size:
2702 1587 731 711
BsaBI GATnn'nnATC
Cuts at: 2808 5725 2808
Size: 2917 2814
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5506
Fragments arranged by size:
5506 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2292 1186
Size: 991 115 4625
Fragments arranged by size:
4625 991 115
BseYI C'CCAG_C
Cuts at: 4934 5430 4934
Size: 496 5235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2666 1163
Size: 573 930 4228
Fragments arranged by size:
4228 930 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2103 3792 3877 4948 2103
Size: 1689 85 1071 2886
Fragments arranged by size:
2886 1689 1071 85
BsmI GAATG_Cn'
Cuts at: 2620 3242 3335 2620
Size: 622 93 5016
Fragments arranged by size:
5016 622 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 3050 3792 3877 4948 1391
Size: 399 313 947 742 85 1071 2174
Fragments arranged by size:
2174 1071 947 742 399 313 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 2482 3620 5350 2482
Size: 1138 1730 2863
Fragments arranged by size:
2863 1730 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 3086 844
Size: 1226 1016 3489
Fragments arranged by size:
3489 1226 1016
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2804 794
Size: 2010 3721
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 3013 3013
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2025 2343 3061 3428 3813 3830 5282 5368
Size: 53 318 718 367 385 17 1452 86
Cuts at: 5368 5380 5726 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 318 86
53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2530 2530
Size: 5731
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2556 487
Size: 683 56 1330 3662
Fragments arranged by size:
3662 1330 683 56
BtsI GCAGTG_nn'
Cuts at: 2647 3247 4041 4061 2647
Size: 600 794 20 4317
Fragments arranged by size:
4317 794 600 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 2922 3035 2922
Size: 113 5618
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2820 3178 4067
Size: 54 1425 50 275 18 990 358 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 990 889 358 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2499 2954 3661 1353
Size: 756 390 455 707 3423
Fragments arranged by size:
3423 756 707 455 390
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2101 2101
Size: 5731
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2469 2600 2798 3794 4391 5178 5446
Size: 393 837 131 198 996 597 787 268
Cuts at: 5446 1239
Size: 1524
Fragments arranged by size:
1524 996 837 787 597 393 268 198
131
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2519 4878 1678
Size: 841 2359 2531
Fragments arranged by size:
2531 2359 841
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2271 2303 4372 4404 4446
Size: 32 135 32 699 32 2069 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 2069 699 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3075 3321 669
Size: 988 281 424 713 246 3079
Fragments arranged by size:
3079 988 713 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 2362 3321 2362
Size: 959 4772
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 2499 3330 2499
Size: 831 4900
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4845 5029 1305
Size: 3540 184 2007
Fragments arranged by size:
3540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 10
Size: 54 1425 343 990 2919
Fragments arranged by size:
2919 1425 990 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3007 3689 4048 4207 518
Size: 648 1841 682 359 159 2042
Fragments arranged by size:
2042 1841 682 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2938 3147 3824 4972 5217 1606
Size: 438 894 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 894 677 438 245 209
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2909 2918 3089 4634 1132
Size: 97 1680 9 171 1545 2229
Fragments arranged by size:
2229 1680 1545 171 97 9
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 2914 4630 2914
Size: 1716 4015
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 3083 830
Size: 2253 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 5731
SacI G_AGCT'C
Cuts at: 2103 2103
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2994 3079 4214 4895 5086 826
Size: 219 1949 85 1135 681 191 1471
Fragments arranged by size:
1949 1471 1135 681 219 191 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 2084 3655 5
Size: 47 2032 1571 2081
Fragments arranged by size:
2081 2032 1571 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5731
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 2837 3170 513
Size: 552 939 390 443 333 3074
Fragments arranged by size:
3074 939 552 443 390 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2696 3758 3775 3928 4113 4558 325
Size: 1255 1116 1062 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1116 1062 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 3977 5689
Size: 274 80 33 51 1443 1041 959 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1712 1443 1041 959 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 2683 1316
Size: 1367 4364
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2876 2876
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 3860 3860
Size: 5731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI