Restriction map for
pFN21AE3885
(Circular) (Six-base) MAPSORT of: pFN21AE3885.seq Check: 8170 from: 1 to: 5050
pFN21AE3885 5050 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2860 278
Size: 53 83 186 936 1324 2468
Fragments arranged by size:
2468 1324 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2393 1937
Size: 456 4594
Acc65I G'GTAC_C
Cuts at: 2371 2371
Size: 5050
AclI AA'CG_TT
Cuts at: 1563 3177 3550 1563
Size: 1614 373 3063
Fragments arranged by size:
3063 1614 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3113 4497 4765 1632
Size: 1481 1384 268 1917
Fragments arranged by size:
1917 1481 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4853
AflIII A'CryG_T
Cuts at: 1203 3949 4926 1203
Size: 2746 977 1327
Fragments arranged by size:
2746 1327 977
AgeI A'CCGG_T
Cuts at: 3881 3881
Size: 5050
AhdI GACnn_n'nnGTC
Cuts at: 3779 3779
Size: 5050
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2296 1184
Size: 1112 3938
AlwNI CAG_nnn'CTG
Cuts at: 2112 2793 4365 2112
Size: 681 1572 2797
Fragments arranged by size:
2797 1572 681
ApaLI G'TGCA_C
Cuts at: 3107 4263 3107
Size: 1156 3894
ApoI r'AATT_y
Cuts at: 1493 2359 2585 2737 4846 1493
Size: 866 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3604 160
Size: 872 2572 1606
Fragments arranged by size:
2572 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5050
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 1870
Size: 73 432 4545
Fragments arranged by size:
4545 432 73
BamHI G'GATC_C
Cuts at: 2380 2747 2380
Size: 367 4683
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2371 3826 618
Size: 325 217 627 230 354 1455 1842
Fragments arranged by size:
1842 1455 627 354 325 230 217
BanII G_rGCy'C
Cuts at: 1391 2369 1391
Size: 978 4072
BbeI G_GCGC'C
Cuts at: 2021 2021
Size: 5050
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3241 3275 1903
Size: 34 50 34 1220 34 3678
Fragments arranged by size:
3678 1220 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2942 4152 2942
Size: 1210 3840
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3661 136
Size: 107 122 71 631 605 1989 1525
Fragments arranged by size:
1989 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5045 5045
Size: 5050
BlpI GC'TnA_GC
Cuts at: 2467 2467
Size: 5050
Bme1580I G_kGCm'C
Cuts at: 1790 3111 4267 1790
Size: 1321 1156 2573
Fragments arranged by size:
2573 1321 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2781 3739 442
Size: 416 53 83 399 24 1364 958 1753
Fragments arranged by size:
1753 1364 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5050
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2141
Size: 5050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 3710 1239
Size: 825 1646 2579
Fragments arranged by size:
2579 1646 825
Bpu10I CC'TnA_GC
Cuts at: 2013 2013
Size: 5050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3175 4040 4338 3175
Size: 865 298 3887
Fragments arranged by size:
3887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3713 882
Size: 617 2214 2219
Fragments arranged by size:
2219 2214 617
BsaAI yAC'GTr
Cuts at: 493 1204 2841 493
Size: 711 1637 2702
Fragments arranged by size:
2702 1637 711
BsaBI GATnn'nnATC
Cuts at: 5044 5044
Size: 5050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2857 3239 3874
Size: 53 83 186 936 485 839 382 635
Cuts at: 3874 275
Size: 1451
Fragments arranged by size:
1451 936 839 635 485 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3483 3881 4155 4302 4713 3483
Size: 398 274 147 411 3820
Fragments arranged by size:
3820 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4825
Fragments arranged by size:
4825 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5050
BseYI C'CCAG_C
Cuts at: 4253 4749 4253
Size: 496 4554
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2500 3261 4289 1540
Size: 275 186 499 761 1028 2301
Fragments arranged by size:
2301 1028 761 499 275 186
BsiHKAI G_wGCw'C
Cuts at: 3111 3196 4267 3111
Size: 85 1071 3894
Fragments arranged by size:
3894 1071 85
BsmI GAATG_Cn'
Cuts at: 2189 2561 2654 2189
Size: 372 93 4585
Fragments arranged by size:
4585 372 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 3111 3196 4267 1391
Size: 399 579 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 85
BspEI T'CCGG_A
Cuts at: 4713 4713
Size: 5050
BspHI T'CATG_A
Cuts at: 2939 4669 2939
Size: 1730 3320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2405 844
Size: 1561 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2937 1394
Size: 429 1114 3507
Fragments arranged by size:
3507 1114 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2217 3545 3719 65
Size: 1183 969 1328 174 1396
Fragments arranged by size:
1396 1328 1183 969 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 3694 3881 1700
Size: 110 210 1674 187 2869
Fragments arranged by size:
2869 1674 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5050
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5050
BssSI C'ACGA_G
Cuts at: 3110 4122 3110
Size: 1012 4038
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5050
BstBI TT'CG_AA
Cuts at: 3854 3854
Size: 5050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5050
BstYI r'GATC_y
Cuts at: 1972 2249 2380 2747 3132 3149 4601 4687 4699
Size: 277 131 367 385 17 1452 86 12
Cuts at: 4699 5045 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 277 131 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4311
Fragments arranged by size:
4311 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 3360 3380 2566
Size: 794 20 4236
Fragments arranged by size:
4236 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5015
DraI TTT'AAA
Cuts at: 2354 2354
Size: 5050
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2117 4057 809
Size: 507 801 1940 1802
Fragments arranged by size:
1940 1802 801 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2497 3386 5015
Size: 54 1425 50 275 18 667 889 1629
Cuts at: 5015 8
Size: 43
Fragments arranged by size:
1629 1425 889 667 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2497 1537
Size: 275 685 4090
Fragments arranged by size:
4090 685 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2980 1353
Size: 1627 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3637 4011 4157 387
Size: 1064 138 2048 374 146 1280
Fragments arranged by size:
2048 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 3113 3710 4497 4765 1239
Size: 393 432 1049 597 787 268 1524
Fragments arranged by size:
1524 1049 787 597 432 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2229 2229
Size: 5050
EcoRI G'AATT_C
Cuts at: 2359 2737 2359
Size: 378 4672
EcoRV GAT'ATC
Cuts at: 2320 2320
Size: 5050
FspI TGC'GCA
Cuts at: 3556 3556
Size: 5050
HaeII r_GCGC'y
Cuts at: 1678 2021 4197 1678
Size: 343 2176 2531
Fragments arranged by size:
2531 2176 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2226 2258 3691 3723 3765
Size: 32 135 32 654 32 1433 32 42
Cuts at: 3765 3797 1373
Size: 32 2626
Fragments arranged by size:
2626 1433 654 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2394 2640 669
Size: 988 281 456 246 3079
Fragments arranged by size:
3079 988 456 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5050
HpaI GTT'AAC
Cuts at: 2640 2640
Size: 5050
KasI G'GCGC_C
Cuts at: 2017 2017
Size: 5050
KpnI G_GTAC'C
Cuts at: 2375 2375
Size: 5050
MfeI C'AATT_G
Cuts at: 2649 2649
Size: 5050
MluI A'CGCG_T
Cuts at: 4926 4926
Size: 5050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 4164 4348 1305
Size: 795 2064 184 2007
Fragments arranged by size:
2064 2007 795 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3228
Fragments arranged by size:
3228 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3008 3367 3526 518
Size: 648 1842 359 159 2042
Fragments arranged by size:
2042 1842 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2466 3143 4291 4536 1606
Size: 565 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 295 245
NarI GG'CG_CC
Cuts at: 2018 2018
Size: 5050
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4498
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5050
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5050
NotI GC'GGCC_GC
Cuts at: 2497 2497
Size: 5050
NspI r_CATG'y
Cuts at: 1132 1229 2095 2408 3953 1132
Size: 97 866 313 1545 2229
Fragments arranged by size:
2229 1545 866 313 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5050
PciI A'CATG_T
Cuts at: 3949 3949
Size: 5050
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5050
PmeI GTTT'AAAC
Cuts at: 2354 2354
Size: 5050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 4632 4664 3119
Size: 32 1481 32 3505
Fragments arranged by size:
3505 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2229 2229
Size: 5050
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5050
PsiI TTA'TAA
Cuts at: 2620 2620
Size: 5050
PstI C_TGCA'G
Cuts at: 830 2402 830
Size: 1572 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5050
PvuII CAG'CTG
Cuts at: 2171 2171
Size: 5050
RsrII CG'GwC_CG
Cuts at: 2057 2057
Size: 5050
SalI G'TCGA_C
Cuts at: 1936 2392 1936
Size: 456 4594
SbfI CC_TGCA'GG
Cuts at: 2402 2402
Size: 5050
ScaI AGT'ACT
Cuts at: 3298 5010 3298
Size: 1712 3338
SfcI C'TryA_G
Cuts at: 826 1045 2224 2398 3533 4214 4405 826
Size: 219 1179 174 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 681 219 191 174
SfoI GGC'GCC
Cuts at: 2019 2019
Size: 5050
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5050
SgrAI Cr'CCGG_yG
Cuts at: 1700 2020 1700
Size: 320 4730
SmaI CCC'GGG
Cuts at: 1872 2377 1872
Size: 505 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3154 4055 4317 820
Size: 197 926 1211 901 262 1553
Fragments arranged by size:
1553 1211 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2841 493
Size: 2348 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5050
SphI G_CATG'C
Cuts at: 1132 2408 1132
Size: 1276 3774
SspI AAT'ATT
Cuts at: 5 52 2974 5
Size: 47 2922 2081
Fragments arranged by size:
2922 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5050
StyI C'CwwG_G
Cuts at: 513 1065 2152 2489 513
Size: 552 1087 337 3074
Fragments arranged by size:
3074 1087 552 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2039 2145 3077 3094 3247 3432 3877
Size: 1255 459 106 932 17 153 185 445
Cuts at: 3877 325
Size: 1498
Fragments arranged by size:
1498 1255 932 459 445 185 153 106
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2294 3296 5008
Size: 274 80 33 51 1443 317 1002 1712
Cuts at: 5008 96
Size: 138
Fragments arranged by size:
1712 1443 1002 317 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5010 5042 5010
Size: 32 5018
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5050
XbaI T'CTAG_A
Cuts at: 2386 2386
Size: 5050
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5050
XmaI C'CCGG_G
Cuts at: 1870 2375 1870
Size: 505 4545
XmnI GAAnn'nnTTC
Cuts at: 3179 3179
Size: 5050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2858 276
Size: 53 83 186 936 1324 2468
Fragments arranged by size:
2468 1324 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI