Restriction map for
pFN21AE3881
(Circular) (Six-base) MAPSORT of: pFN21AE3881.seq Check: 661 from: 1 to: 6061
pFN21AE3881 6061 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3404 1937
Size: 1467 4594
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 3204 4124 5508 5776 1632
Size: 1328 244 920 1384 268 1917
Fragments arranged by size:
1917 1384 1328 920 268 244
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2842 1184
Size: 1658 4403
AlwNI CAG_nnn'CTG
Cuts at: 3280 3804 5376 3280
Size: 524 1572 3965
Fragments arranged by size:
3965 1572 524
ApaLI G'TGCA_C
Cuts at: 4118 5274 4118
Size: 1156 4905
ApoI r'AATT_y
Cuts at: 1493 2391 2595 3370 3596 3748 5857 1493
Size: 898 204 775 226 152 2109 1697
Fragments arranged by size:
2109 1697 898 775 226 204 152
AseI AT'TA_AT
Cuts at: 160 1032 2214 4615 160
Size: 872 1182 2401 1606
Fragments arranged by size:
2401 1606 1182 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 3391 3758 3391
Size: 367 5694
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 4837 618
Size: 325 217 627 1595 1455 1842
Fragments arranged by size:
1842 1595 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3380 1391
Size: 1989 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2072 928
Size: 971 173 4917
Fragments arranged by size:
4917 971 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3131 6056 3131
Size: 2925 3136
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 4122 5278 1790
Size: 2332 1156 2573
Fragments arranged by size:
2573 2332 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4721 1239
Size: 3482 2579
Bpu10I CC'TnA_GC
Cuts at: 2907 2907
Size: 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3338 4186 5051 5349 3338
Size: 848 865 298 4050
Fragments arranged by size:
4050 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3852 493
Size: 711 2648 2702
Fragments arranged by size:
2702 2648 711
BsaBI GATnn'nnATC
Cuts at: 2040 6055 2040
Size: 4015 2046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6061
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3249 1163
Size: 573 1513 3975
Fragments arranged by size:
3975 1513 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2535 4122 4207 5278 2535
Size: 1587 85 1071 3318
Fragments arranged by size:
3318 1587 1071 85
BsmI GAATG_Cn'
Cuts at: 2911 3180 3572 3665 2911
Size: 269 392 93 5307
Fragments arranged by size:
5307 392 269 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 3380 4122 4207 5278 1391
Size: 399 745 845 742 85 1071 2174
Fragments arranged by size:
2174 1071 845 745 742 399 85
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3156 3950 5680 3156
Size: 794 1730 3537
Fragments arranged by size:
3537 1730 794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2725 3948 1394
Size: 429 902 1223 3507
Fragments arranged by size:
3507 1223 902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4556 4730 65
Size: 1183 3308 174 1396
Fragments arranged by size:
3308 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 4121 5133 4121
Size: 1012 5049
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3280 794
Size: 2486 3575
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2681 2681
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 3131 3391 3758 4143 4160 5612 5698 5710
Size: 1159 260 367 385 17 1452 86 12
Cuts at: 5710 6056 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1159 385 367 346 260 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3100 3577 4371 4391 3100
Size: 477 794 20 4770
Fragments arranged by size:
4770 794 477 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3120 3365 3120
Size: 245 5816
DraIII CAC_nnn'GTG
Cuts at: 2959 2959
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2440 2671 2683
Size: 54 1425 50 275 18 610 231 12
Cuts at: 2683 3508 4397 6026 8
Size: 825 889 1629 43
Fragments arranged by size:
1629 1425 889 825 610 275 231 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3991 1353
Size: 2638 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4648 5022 5168 387
Size: 1064 138 3059 374 146 1280
Fragments arranged by size:
3059 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2960 3204 4124 4721 5508 5776 1239
Size: 393 1328 244 920 597 787 268 1524
Fragments arranged by size:
1524 1328 920 787 597 393 268 244
EcoO109I rG'GnC_Cy
Cuts at: 2571 2571
Size: 6061
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
EcoRV GAT'ATC
Cuts at: 2707 2876 2707
Size: 169 5892
FspI TGC'GCA
Cuts at: 2051 4567 2051
Size: 2516 3545
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2966 2998 4702 4734 4776
Size: 32 135 32 1394 32 1704 32 42
Cuts at: 4776 4808 1373
Size: 32 2626
Fragments arranged by size:
2626 1704 1394 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6061
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3333 3660 3333
Size: 327 5734
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3187 5175 5359 1305
Size: 1882 1988 184 2007
Fragments arranged by size:
2007 1988 1882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2442 2673 2685 10
Size: 54 1425 343 610 231 12 3386
Fragments arranged by size:
3386 1425 610 343 231 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2673 4019 4378 4537 518
Size: 648 843 664 1346 359 159 2042
Fragments arranged by size:
2042 1346 843 664 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3477 4154 5302 5547 1606
Size: 1871 677 1148 245 2120
Fragments arranged by size:
2120 1871 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
NdeI CA'TA_TG
Cuts at: 387 3061 387
Size: 2674 3387
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 3419 4964 1132
Size: 97 2190 1545 2229
Fragments arranged by size:
2229 2190 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PstI C_TGCA'G
Cuts at: 830 3292 3413 830
Size: 2462 121 3478
Fragments arranged by size:
3478 2462 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 4309 6021 4309
Size: 1712 4349
SfcI C'TryA_G
Cuts at: 826 1045 2232 2566 3288 3409 4544 5225 5416
Size: 219 1187 334 722 121 1135 681 191
Cuts at: 5416 826
Size: 1471
Fragments arranged by size:
1471 1187 1135 722 681 334 219 191
121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3317 4165 5066 5328 820
Size: 197 926 1374 848 901 262 1553
Fragments arranged by size:
1553 1374 926 901 848 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3196 3985 5
Size: 47 3144 789 2081
Fragments arranged by size:
3144 2081 789 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2004 3500 513
Size: 552 939 1496 3074
Fragments arranged by size:
3074 1496 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4088 4105 4258 4443 4888 325
Size: 1255 2508 17 153 185 445 1498
Fragments arranged by size:
2508 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2150 4307 6019
Size: 274 80 33 51 1443 173 2157 1712
Cuts at: 6019 96
Size: 138
Fragments arranged by size:
2157 1712 1443 274 173 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2941 2973 6021 6053 2941
Size: 32 3048 32 2949
Fragments arranged by size:
3048 2949 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 2398 3397 2398
Size: 999 5062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3199 3199
Size: 6061
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2068 2469 4190 2068
Size: 401 1721 3939
Fragments arranged by size:
3939 1721 401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI