Restriction map for
pFN21AE3879
(Circular) (Six-base) MAPSORT of: pFN21AE3879.seq Check: 4497 from: 1 to: 7633
pFN21AE3879 7633 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5443 278
Size: 53 83 186 936 3907 2468
Fragments arranged by size:
3907 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4976 1937
Size: 3039 4594
Acc65I G'GTAC_C
Cuts at: 4954 4954
Size: 7633
AclI AA'CG_TT
Cuts at: 1563 5760 6133 1563
Size: 4197 373 3063
Fragments arranged by size:
4197 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 3093 3396 3842 4853 5696 7080 7348
Size: 519 942 303 446 1011 843 1384 268
Cuts at: 7348 1632
Size: 1917
Fragments arranged by size:
1917 1384 1011 942 843 519 446 303
268
AflII C'TTAA_G
Cuts at: 820 1017 3536 820
Size: 197 2519 4917
Fragments arranged by size:
4917 2519 197
AflIII A'CryG_T
Cuts at: 1203 6532 7509 1203
Size: 5329 977 1327
Fragments arranged by size:
5329 1327 977
AgeI A'CCGG_T
Cuts at: 6464 6464
Size: 7633
AhdI GACnn_n'nnGTC
Cuts at: 6362 6362
Size: 7633
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7633
AlwNI CAG_nnn'CTG
Cuts at: 2086 2201 2378 2455 5376 6948 2086
Size: 115 177 77 2921 1572 2771
Fragments arranged by size:
2921 2771 1572 177 115 77
ApaLI G'TGCA_C
Cuts at: 5690 6846 5690
Size: 1156 6477
ApoI r'AATT_y
Cuts at: 1493 2296 2704 3140 3971 4419 4471 4664 4942
Size: 803 408 436 831 448 52 193 278
Cuts at: 4942 5168 5320 7429 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 803 448 436 408 278
226 193 152 52
AseI AT'TA_AT
Cuts at: 160 1032 3648 4674 6187 160
Size: 872 2616 1026 1513 1606
Fragments arranged by size:
2616 1606 1513 1026 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7633
AvaI C'yCGr_G
Cuts at: 1870 1943 4958 1870
Size: 73 3015 4545
Fragments arranged by size:
4545 3015 73
BamHI G'GATC_C
Cuts at: 4963 5330 4963
Size: 367 7266
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4954 6409 618
Size: 325 217 627 3167 1455 1842
Fragments arranged by size:
3167 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3594 4952 1391
Size: 2203 1358 4072
Fragments arranged by size:
4072 2203 1358
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3404 4583 928
Size: 971 1505 1179 3978
Fragments arranged by size:
3978 1505 1179 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5824 5858 1903
Size: 34 3887 34 3678
Fragments arranged by size:
3887 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5525 6735 5525
Size: 1210 6423
BclI T'GATC_A
Cuts at: 1548 4486 1548
Size: 2938 4695
BfrBI ATG'CAT
Cuts at: 2722 2722
Size: 7633
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6244 136
Size: 107 122 71 631 605 4572 1525
Fragments arranged by size:
4572 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2579 3505 7628 2579
Size: 926 4123 2584
Fragments arranged by size:
4123 2584 926
BlpI GC'TnA_GC
Cuts at: 5050 5050
Size: 7633
Bme1580I G_kGCm'C
Cuts at: 1790 5694 6850 1790
Size: 3904 1156 2573
Fragments arranged by size:
3904 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7633
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5364 6322 442
Size: 416 53 83 399 24 3947 958 1753
Fragments arranged by size:
3947 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6293 1239
Size: 5054 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 3611 5758 6623 6921 2255
Size: 1356 2147 865 298 2967
Fragments arranged by size:
2967 2147 1356 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6296 882
Size: 617 4797 2219
Fragments arranged by size:
4797 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5424 493
Size: 711 4220 2702
Fragments arranged by size:
4220 2702 711
BsaBI GATnn'nnATC
Cuts at: 2196 2825 7627 2196
Size: 629 4802 2202
Fragments arranged by size:
4802 2202 629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5440 5822 6457 275
Size: 53 83 186 936 3907 382 635 1451
Fragments arranged by size:
3907 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6066 6464 6738 6885 7296 6066
Size: 398 274 147 411 6403
Fragments arranged by size:
6403 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7408
Fragments arranged by size:
7408 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7633
BseYI C'CCAG_C
Cuts at: 6836 7332 6836
Size: 496 7137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7060
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5083 5844 6872 1540
Size: 275 186 3082 761 1028 2301
Fragments arranged by size:
3082 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3594 5694 5779 6850 3594
Size: 2100 85 1071 4377
Fragments arranged by size:
4377 2100 1071 85
BsmI GAATG_Cn'
Cuts at: 5144 5237 5144
Size: 93 7540
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3594 4952 5694 5779 6850 1391
Size: 399 1804 1358 742 85 1071 2174
Fragments arranged by size:
2174 1804 1358 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7296 7296
Size: 7633
BspHI T'CATG_A
Cuts at: 5522 7252 5522
Size: 1730 5903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 2794 4988 844
Size: 1386 564 2194 3489
Fragments arranged by size:
3489 2194 1386 564
BsrBI CCG'CTC
Cuts at: 1394 1823 5520 1394
Size: 429 3697 3507
Fragments arranged by size:
3697 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 6128 6302 65
Size: 1183 3305 1575 174 1396
Fragments arranged by size:
3305 1575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6277 6464 1700
Size: 110 4467 187 2869
Fragments arranged by size:
4467 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7633
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7633
BssSI C'ACGA_G
Cuts at: 5693 6705 5693
Size: 1012 6621
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7633
BstBI TT'CG_AA
Cuts at: 6437 6437
Size: 7633
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7633
BstYI r'GATC_y
Cuts at: 1972 2197 2579 2641 3505 4823 4963 5330 5715
Size: 225 382 62 864 1318 140 367 385
Cuts at: 5715 5732 7184 7270 7282 7628 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1318 864 385 382 367 346
225 140 86 62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3231 513
Size: 552 939 1227 4915
Fragments arranged by size:
4915 1227 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4123 487
Size: 683 56 2897 3997
Fragments arranged by size:
3997 2897 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 5149 5943 5963 3559
Size: 1590 794 20 5229
Fragments arranged by size:
5229 1590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7598
DraI TTT'AAA
Cuts at: 4937 4937
Size: 7633
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6640 809
Size: 507 5324 1802
Fragments arranged by size:
5324 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5080 5969 7598
Size: 54 1425 50 275 18 3250 889 1629
Cuts at: 7598 8
Size: 43
Fragments arranged by size:
3250 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5080 1537
Size: 275 3268 4090
Fragments arranged by size:
4090 3268 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3258 3741 4038 4314 4719 5563 1353
Size: 1905 483 297 276 405 844 3423
Fragments arranged by size:
3423 1905 844 483 405 297 276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6220 6594 6740 387
Size: 1064 138 4631 374 146 1280
Fragments arranged by size:
4631 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3592 3592
Size: 7633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 3093 3396 3842 4853 5696 6293
Size: 393 519 942 303 446 1011 843 597
Cuts at: 6293 7080 7348 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1011 942 843 787 597 519 446
393 303 268
EcoO109I rG'GnC_Cy
Cuts at: 4798 4798
Size: 7633
EcoRI G'AATT_C
Cuts at: 2296 2704 4942 5320 2296
Size: 408 2238 378 4609
Fragments arranged by size:
4609 2238 408 378
EcoRV GAT'ATC
Cuts at: 4023 4023
Size: 7633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3317 4052 4298 3317
Size: 735 246 6652
Fragments arranged by size:
6652 735 246
FspI TGC'GCA
Cuts at: 6139 6139
Size: 7633
HaeII r_GCGC'y
Cuts at: 1678 6780 1678
Size: 5102 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6274 6306 6348 6380 1373
Size: 32 135 32 4702 32 42 32 2626
Fragments arranged by size:
4702 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 4115 4977 5223 669
Size: 988 281 1521 656 862 246 3079
Fragments arranged by size:
3079 1521 988 862 656 281 246
HindIII A'AGCT_T
Cuts at: 748 3275 3533 3569 748
Size: 2527 258 36 4812
Fragments arranged by size:
4812 2527 258 36
HpaI GTT'AAC
Cuts at: 3459 5223 3459
Size: 1764 5869
KpnI G_GTAC'C
Cuts at: 4958 4958
Size: 7633
MfeI C'AATT_G
Cuts at: 2943 3215 5232 2943
Size: 272 2017 5344
Fragments arranged by size:
5344 2017 272
MluI A'CGCG_T
Cuts at: 7509 7509
Size: 7633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2779 6747 6931 1305
Size: 1474 3968 184 2007
Fragments arranged by size:
3968 2007 1474 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5811
Fragments arranged by size:
5811 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4164 4776 5591 5950 6109 518
Size: 648 2998 612 815 359 159 2042
Fragments arranged by size:
2998 2042 815 648 612 359 159
MspA1I CmG'CkG
Cuts at: 1606 5049 5726 6874 7119 1606
Size: 3443 677 1148 245 2120
Fragments arranged by size:
3443 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3231 513
Size: 552 939 1227 4915
Fragments arranged by size:
4915 1227 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7633
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7633
NotI GC'GGCC_GC
Cuts at: 5080 5080
Size: 7633
NsiI A_TGCA'T
Cuts at: 2724 2724
Size: 7633
NspI r_CATG'y
Cuts at: 1132 1229 4991 6536 1132
Size: 97 3762 1545 2229
Fragments arranged by size:
3762 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2086 1348
Size: 738 6895
PciI A'CATG_T
Cuts at: 6532 6532
Size: 7633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7633
PmeI GTTT'AAAC
Cuts at: 4937 4937
Size: 7633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5702 5734 7215 7247 5702
Size: 32 1481 32 6088
Fragments arranged by size:
6088 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7633
PsiI TTA'TAA
Cuts at: 4209 5203 4209
Size: 994 6639
PstI C_TGCA'G
Cuts at: 830 2587 3161 4985 830
Size: 1757 574 1824 3478
Fragments arranged by size:
3478 1824 1757 574
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7633
SacI G_AGCT'C
Cuts at: 3594 3594
Size: 7633
SalI G'TCGA_C
Cuts at: 1936 4975 1936
Size: 3039 4594
SapI GCTCTTCn'nnn_
Cuts at: 3741 3741
Size: 7633
SbfI CC_TGCA'GG
Cuts at: 4985 4985
Size: 7633
ScaI AGT'ACT
Cuts at: 3013 5881 7593 3013
Size: 2868 1712 3053
Fragments arranged by size:
3053 2868 1712
SfcI C'TryA_G
Cuts at: 826 1045 2583 3157 4598 4981 6116 6797 6988
Size: 219 1538 574 1441 383 1135 681 191
Cuts at: 6988 826
Size: 1471
Fragments arranged by size:
1538 1471 1441 1135 681 574 383 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7633
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7633
SmaI CCC'GGG
Cuts at: 1872 4960 1872
Size: 3088 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 3536 3626 5737 6638 6900
Size: 197 926 291 1302 90 2111 901 262
Cuts at: 6900 820
Size: 1553
Fragments arranged by size:
2111 1553 1302 926 901 291 262 197
90
SnaBI TAC'GTA
Cuts at: 493 5424 493
Size: 4931 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7633
SphI G_CATG'C
Cuts at: 1132 4991 1132
Size: 3859 3774
SspI AAT'ATT
Cuts at: 5 52 5557 5
Size: 47 5505 2081
Fragments arranged by size:
5505 2081 47
StuI AGG'CCT
Cuts at: 1517 3875 1517
Size: 2358 5275
StyI C'CwwG_G
Cuts at: 513 1065 2004 3231 5072 513
Size: 552 939 1227 1841 3074
Fragments arranged by size:
3074 1841 1227 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5660 5677 5830 6015 6460 325
Size: 1255 4080 17 153 185 445 1498
Fragments arranged by size:
4080 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2126 2369 3011
Size: 274 80 33 51 1443 149 243 642
Cuts at: 3011 3436 5879 7591 96
Size: 425 2443 1712 138
Fragments arranged by size:
2443 1712 1443 642 425 274 243 149
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7593 7625 7593
Size: 32 7601
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7633
XbaI T'CTAG_A
Cuts at: 4969 4969
Size: 7633
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7633
XmaI C'CCGG_G
Cuts at: 1870 4958 1870
Size: 3088 4545
XmnI GAAnn'nnTTC
Cuts at: 3696 3855 5762 3696
Size: 159 1907 5567
Fragments arranged by size:
5567 1907 159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5441 276
Size: 53 83 186 936 3907 2468
Fragments arranged by size:
3907 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI