Restriction map for
pFN21AE3873
(Circular) (Six-base) MAPSORT of: pFN21AE3873.seq Check: 7108 from: 1 to: 6157
pFN21AE3873 6157 bp
With 182 enzymes: SgfI *
August 4, 2021 21:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2097 3967 278
Size: 53 83 186 936 561 1870 2468
Fragments arranged by size:
2468 1870 936 561 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3500 1937
Size: 1563 4594
Acc65I G'GTAC_C
Cuts at: 3478 3478
Size: 6157
AclI AA'CG_TT
Cuts at: 1563 4284 4657 1563
Size: 2721 373 3063
Fragments arranged by size:
3063 2721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3125 4220 5604 5872 1632
Size: 1493 1095 1384 268 1917
Fragments arranged by size:
1917 1493 1384 1095 268
AfeI AGC'GCT
Cuts at: 2723 2723
Size: 6157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5960
AflIII A'CryG_T
Cuts at: 1203 2503 5056 6033 1203
Size: 1300 2553 977 1327
Fragments arranged by size:
2553 1327 1300 977
AgeI A'CCGG_T
Cuts at: 4988 4988
Size: 6157
AhdI GACnn_n'nnGTC
Cuts at: 4886 4886
Size: 6157
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6157
AlwNI CAG_nnn'CTG
Cuts at: 2214 2374 3098 3154 3157 3310 3900 5472 2214
Size: 160 724 56 3 153 590 1572 2899
Fragments arranged by size:
2899 1572 724 590 160 153 56 3
ApaLI G'TGCA_C
Cuts at: 4214 5370 4214
Size: 1156 5001
ApoI r'AATT_y
Cuts at: 1493 3466 3692 3844 5953 1493
Size: 1973 226 152 2109 1697
Fragments arranged by size:
2109 1973 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4711 160
Size: 872 3679 1606
Fragments arranged by size:
3679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 2649 2922 3133 3337 3482 1870
Size: 73 84 622 273 211 204 145 4545
Fragments arranged by size:
4545 622 273 211 204 145 84 73
BamHI G'GATC_C
Cuts at: 3487 3854 3487
Size: 367 5790
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2524 3478 4933 618
Size: 325 217 627 737 954 1455 1842
Fragments arranged by size:
1842 1455 954 737 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2252 3233 3476 1391
Size: 861 981 243 4072
Fragments arranged by size:
4072 981 861 243
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4348 4382 1903
Size: 34 2411 34 3678
Fragments arranged by size:
3678 2411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4049 5259 4049
Size: 1210 4947
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4768 136
Size: 107 122 71 631 605 3096 1525
Fragments arranged by size:
3096 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2414 6152 2414
Size: 3738 2419
BlpI GC'TnA_GC
Cuts at: 2148 3574 2148
Size: 1426 4731
Bme1580I G_kGCm'C
Cuts at: 1790 2028 2125 2240 2529 2990 4218 5374 1790
Size: 238 97 115 289 461 1228 1156 2573
Fragments arranged by size:
2573 1228 1156 461 289 238 115 97
BmgBI CAC'GTC
Cuts at: 1318 2521 1318
Size: 1203 4954
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2234 3117 3888
Size: 416 53 83 399 24 817 883 771
Cuts at: 3888 4846 442
Size: 958 1753
Fragments arranged by size:
1753 958 883 817 771 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2163 2743 3196 2163
Size: 580 453 5124
Fragments arranged by size:
5124 580 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2274 2396 2722 2888 3050 3083 3299
Size: 884 151 122 326 166 162 33 216
Cuts at: 3299 4817 1239
Size: 1518 2579
Fragments arranged by size:
2579 1518 884 326 216 166 162 151
122 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3209 4282 5147 5445 3209
Size: 1073 865 298 3921
Fragments arranged by size:
3921 1073 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4820 882
Size: 617 3321 2219
Fragments arranged by size:
3321 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2023 3948 493
Size: 711 819 1925 2702
Fragments arranged by size:
2702 1925 819 711
BsaBI GATnn'nnATC
Cuts at: 2642 6151 2642
Size: 3509 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 3964 4346 4981
Size: 53 83 186 936 561 1870 382 635
Cuts at: 4981 275
Size: 1451
Fragments arranged by size:
1870 1451 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2992 3414 4590 4988 5262 5409 5820 2992
Size: 422 1176 398 274 147 411 3329
Fragments arranged by size:
3329 1176 422 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 1178
Size: 30 165 30 1038 30 4864
Fragments arranged by size:
4864 1038 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2246 2716 3188 3296 1186
Size: 934 126 470 472 108 4047
Fragments arranged by size:
4047 934 472 470 126 108
BseYI C'CCAG_C
Cuts at: 2360 2367 2833 3091 3157 5360 5856 2360
Size: 7 466 258 66 2203 496 2661
Fragments arranged by size:
2661 2203 496 466 258 66 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2267 2774 1163
Size: 573 531 507 4546
Fragments arranged by size:
4546 573 531 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3607 4368 5396 1540
Size: 275 186 1606 761 1028 2301
Fragments arranged by size:
2301 1606 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 3048 3180 3233 3402 4218 4303 5374 2691
Size: 357 132 53 169 816 85 1071 3474
Fragments arranged by size:
3474 1071 816 357 169 132 85 53
BsmI GAATG_Cn'
Cuts at: 3668 3761 3668
Size: 93 6064
BsmBI CGTCTCn'nnnn_
Cuts at: 2101 2915 2101
Size: 814 5343
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2125 2240 2252 2529 2691 2990
Size: 399 238 97 115 12 277 162 299
Cuts at: 2990 3048 3180 3233 3402 3476 4218 4303 5374
Size: 58 132 53 169 74 742 85 1071
Cuts at: 5374 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 299 277 238 169
162 132 115 97 85 74 58 53
12
BspEI T'CCGG_A
Cuts at: 5820 5820
Size: 6157
BspHI T'CATG_A
Cuts at: 4046 5776 4046
Size: 1730 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3512 844
Size: 2668 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2736 3193 4044 1394
Size: 429 913 457 851 3507
Fragments arranged by size:
3507 913 851 457 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 2742 4652 4826 65
Size: 1183 1063 431 1910 174 1396
Fragments arranged by size:
1910 1396 1183 1063 431 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3375 3432 4801 4988 1700
Size: 110 1565 57 1369 187 2869
Fragments arranged by size:
2869 1565 1369 187 110 57
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6157
BssSI C'ACGA_G
Cuts at: 4217 5229 4217
Size: 1012 5145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6157
BstBI TT'CG_AA
Cuts at: 4961 4961
Size: 6157
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6157
BstXI CCAn_nnnn'nTGG
Cuts at: 2252 2252
Size: 6157
BstYI r'GATC_y
Cuts at: 1972 2414 2637 3251 3487 3854 4239 4256 5708
Size: 442 223 614 236 367 385 17 1452
Cuts at: 5708 5794 5806 6152 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 614 442 385 367 346 236
223 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 487
Size: 683 56 1106 4312
Fragments arranged by size:
4312 1106 683 56
BtsI GCAGTG_nn'
Cuts at: 3673 4467 4487 3673
Size: 794 20 5343
Fragments arranged by size:
5343 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6157
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6122
DraI TTT'AAA
Cuts at: 3461 3461
Size: 6157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2093 3114 5164 809
Size: 507 777 1021 2050 1802
Fragments arranged by size:
2050 1802 1021 777 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2784 2844 3604
Size: 54 1425 50 275 18 954 60 760
Cuts at: 3604 4493 6122 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 954 889 760 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3604 1537
Size: 275 1792 4090
Fragments arranged by size:
4090 1792 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 4087 1353
Size: 1256 1478 3423
Fragments arranged by size:
3423 1478 1256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2454 2513 4744 5118 5264 387
Size: 1064 138 865 59 2231 374 146 1280
Fragments arranged by size:
2231 1280 1064 865 374 146 138 59
EcoICRI GAG'CTC
Cuts at: 3231 3231
Size: 6157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2274 2396 2722 2888 3050 3083
Size: 393 491 151 122 326 166 162 33
Cuts at: 3083 3125 3299 4220 4817 5604 5872 1239
Size: 42 174 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 597 491 393 326 268
174 166 162 151 122 42 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2105 3281 2105
Size: 1176 4981
EcoO109I rG'GnC_Cy
Cuts at: 3116 3307 3116
Size: 191 5966
EcoRI G'AATT_C
Cuts at: 3466 3844 3466
Size: 378 5779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 3395 2620
Size: 775 5382
FspI TGC'GCA
Cuts at: 4663 4663
Size: 6157
HaeII r_GCGC'y
Cuts at: 1678 2374 2725 5304 1678
Size: 696 351 2579 2531
Fragments arranged by size:
2579 2531 696 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2673 2705 4798 4830 4872
Size: 32 135 32 1101 32 2093 32 42
Cuts at: 4872 4904 1373
Size: 32 2626
Fragments arranged by size:
2626 2093 1101 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 3501 3747 669
Size: 988 281 322 1241 246 3079
Fragments arranged by size:
3079 1241 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6157
HpaI GTT'AAC
Cuts at: 3747 3747
Size: 6157
KpnI G_GTAC'C
Cuts at: 3482 3482
Size: 6157
MfeI C'AATT_G
Cuts at: 3756 3756
Size: 6157
MluI A'CGCG_T
Cuts at: 6033 6033
Size: 6157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2790 5271 5455 1305
Size: 1485 2481 184 2007
Fragments arranged by size:
2481 2007 1485 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 2846 10
Size: 54 1425 343 954 60 3321
Fragments arranged by size:
3321 1425 954 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 4115 4474 4633 518
Size: 648 1419 1530 359 159 2042
Fragments arranged by size:
2042 1530 1419 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2842 3028 3154 3573 4250 5398 5643 1606
Size: 1236 186 126 419 677 1148 245 2120
Fragments arranged by size:
2120 1236 1148 677 419 245 186 126
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5605
NdeI CA'TA_TG
Cuts at: 387 2515 2968 387
Size: 2128 453 3576
Fragments arranged by size:
3576 2128 453
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6157
NotI GC'GGCC_GC
Cuts at: 3604 3604
Size: 6157
NspI r_CATG'y
Cuts at: 1132 1229 2507 3515 5060 1132
Size: 97 1278 1008 1545 2229
Fragments arranged by size:
2229 1545 1278 1008 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6157
PciI A'CATG_T
Cuts at: 2503 5056 2503
Size: 2553 3604
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 3098 1108
Size: 1266 724 4167
Fragments arranged by size:
4167 1266 724
PmeI GTTT'AAAC
Cuts at: 3461 3461
Size: 6157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 5739 5771 4226
Size: 32 1481 32 4612
Fragments arranged by size:
4612 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3116 3307 3116
Size: 191 5966
PshAI GACnn'nnGTC
Cuts at: 1613 2204 1613
Size: 591 5566
PsiI TTA'TAA
Cuts at: 3727 3727
Size: 6157
PstI C_TGCA'G
Cuts at: 830 2799 3509 830
Size: 1969 710 3478
Fragments arranged by size:
3478 1969 710
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6157
PvuII CAG'CTG
Cuts at: 2842 3028 3154 2842
Size: 186 126 5845
Fragments arranged by size:
5845 186 126
SacI G_AGCT'C
Cuts at: 3233 3233
Size: 6157
SalI G'TCGA_C
Cuts at: 1936 3499 1936
Size: 1563 4594
SbfI CC_TGCA'GG
Cuts at: 3509 3509
Size: 6157
ScaI AGT'ACT
Cuts at: 4405 6117 4405
Size: 1712 4445
SfcI C'TryA_G
Cuts at: 826 1045 2795 3505 4640 5321 5512 826
Size: 219 1750 710 1135 681 191 1471
Fragments arranged by size:
1750 1471 1135 710 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6157
SmaI CCC'GGG
Cuts at: 1872 2029 2924 3135 3484 1872
Size: 157 895 211 349 4545
Fragments arranged by size:
4545 895 349 211 157
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3188 4261 5162 5424 820
Size: 197 926 1245 1073 901 262 1553
Fragments arranged by size:
1553 1245 1073 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3948 493
Size: 3455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6157
SphI G_CATG'C
Cuts at: 1132 3515 1132
Size: 2383 3774
SspI AAT'ATT
Cuts at: 5 52 4081 5
Size: 47 4029 2081
Fragments arranged by size:
4029 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6157
StyI C'CwwG_G
Cuts at: 513 1065 3437 3444 3596 513
Size: 552 2372 7 152 3074
Fragments arranged by size:
3074 2372 552 152 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2847 3105 4184 4201 4354 4539 4984
Size: 1255 1267 258 1079 17 153 185 445
Cuts at: 4984 325
Size: 1498
Fragments arranged by size:
1498 1267 1255 1079 445 258 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 4403 6115
Size: 274 80 33 51 1443 321 2105 1712
Cuts at: 6115 96
Size: 138
Fragments arranged by size:
2105 1712 1443 321 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 6117 6149 2219
Size: 32 3866 32 2227
Fragments arranged by size:
3866 2227 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6157
XbaI T'CTAG_A
Cuts at: 3493 3493
Size: 6157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2854 3095 2539
Size: 315 241 5601
Fragments arranged by size:
5601 315 241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6157
XmaI C'CCGG_G
Cuts at: 1870 2027 2922 3133 3482 1870
Size: 157 895 211 349 4545
Fragments arranged by size:
4545 895 349 211 157
XmnI GAAnn'nnTTC
Cuts at: 2505 4286 2505
Size: 1781 4376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2095 3965 276
Size: 53 83 186 936 561 1870 2468
Fragments arranged by size:
2468 1870 936 561 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI