Restriction map for
pFN21AE3848
(Circular) (Six-base) MAPSORT of: pFN21AE3848.seq Check: 7512 from: 1 to: 7102
pFN21AE3848 7102 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2874 2973 3333 3615 2874
Size: 99 360 282 6361
Fragments arranged by size:
6361 360 282 99
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4912 278
Size: 53 83 186 936 3376 2468
Fragments arranged by size:
3376 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4445 1937
Size: 2508 4594
Acc65I G'GTAC_C
Cuts at: 4423 4423
Size: 7102
AclI AA'CG_TT
Cuts at: 1563 5229 5602 1563
Size: 3666 373 3063
Fragments arranged by size:
3666 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 5165 6549 6817 1632
Size: 1244 2289 1384 268 1917
Fragments arranged by size:
2289 1917 1384 1244 268
AflII C'TTAA_G
Cuts at: 820 1017 3406 820
Size: 197 2389 4516
Fragments arranged by size:
4516 2389 197
AflIII A'CryG_T
Cuts at: 1203 6001 6978 1203
Size: 4798 977 1327
Fragments arranged by size:
4798 1327 977
AgeI A'CCGG_T
Cuts at: 5933 5933
Size: 7102
AhdI GACnn_n'nnGTC
Cuts at: 3979 5831 3979
Size: 1852 5250
AleI CACnn'nnGTG
Cuts at: 1166 2655 3032 1166
Size: 1489 377 5236
Fragments arranged by size:
5236 1489 377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3954 1184
Size: 2770 4332
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3065 3097 3065
Size: 32 7070
AlwNI CAG_nnn'CTG
Cuts at: 2336 2518 3259 4845 6417 2336
Size: 182 741 1586 1572 3021
Fragments arranged by size:
3021 1586 1572 741 182
ApaI G_GGCC'C
Cuts at: 2153 2169 2210 2366 2423 2765 2153
Size: 16 41 156 57 342 6490
Fragments arranged by size:
6490 342 156 57 41 16
ApaLI G'TGCA_C
Cuts at: 5159 6315 5159
Size: 1156 5946
ApoI r'AATT_y
Cuts at: 1493 3318 3748 4411 4637 4789 6898 1493
Size: 1825 430 663 226 152 2109 1697
Fragments arranged by size:
2109 1825 1697 663 430 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5656 160
Size: 872 4624 1606
Fragments arranged by size:
4624 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7102
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2168 2365 2653 2813 3158 4427
Size: 73 202 23 197 288 160 345 1269
Cuts at: 4427 1870
Size: 4545
Fragments arranged by size:
4545 1269 345 288 202 197 160 73
23
BamHI G'GATC_C
Cuts at: 4432 4799 4432
Size: 367 6735
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 2961 3131 3930 4423
Size: 325 217 627 935 239 170 799 493
Cuts at: 4423 5878 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 935 799 627 493 325 239
217 170
BanII G_rGCy'C
Cuts at: 1391 2153 2169 2210 2318 2366 2373 2423 2700
Size: 762 16 41 108 48 7 50 277
Cuts at: 2700 2765 2783 3917 4067 4421 1391
Size: 65 18 1134 150 354 4072
Fragments arranged by size:
4072 1134 762 354 277 150 108 65
50 48 41 18 16 7
BbeI G_GCGC'C
Cuts at: 2726 3135 2726
Size: 409 6693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3203 3692 928
Size: 971 1304 489 4338
Fragments arranged by size:
4338 1304 971 489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3133 3167 5293 5327 1903
Size: 34 1196 34 2126 34 3678
Fragments arranged by size:
3678 2126 1196 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2075 4012 4994 6204 2075
Size: 1937 982 1210 2973
Fragments arranged by size:
2973 1937 1210 982
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7102
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2173 2195 5713
Size: 107 122 71 631 605 501 22 3518
Cuts at: 5713 136
Size: 1525
Fragments arranged by size:
3518 1525 631 605 501 122 107 71
22
BglII A'GATC_T
Cuts at: 7097 7097
Size: 7102
BlpI GC'TnA_GC
Cuts at: 3732 4519 3732
Size: 787 6315
Bme1580I G_kGCm'C
Cuts at: 1790 2153 2169 2210 2366 2423 2495 2765 2813
Size: 363 16 41 156 57 72 270 48
Cuts at: 2813 3452 3524 3933 5163 6319 1790
Size: 639 72 409 1230 1156 2573
Fragments arranged by size:
2573 1230 1156 639 409 363 270 156
72 72 57 48 41 16
BmgBI CAC'GTC
Cuts at: 1318 2302 1318
Size: 984 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4309 4833 5791
Size: 416 53 83 399 24 2892 524 958
Cuts at: 5791 442
Size: 1753
Fragments arranged by size:
2892 1753 958 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2693 1056
Size: 1637 5465
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3936 3936
Size: 7102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 4126 5762 1239
Size: 1118 1769 1636 2579
Fragments arranged by size:
2579 1769 1636 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2939 3029 4072 5227 6092 6390 2939
Size: 90 1043 1155 865 298 3651
Fragments arranged by size:
3651 1155 1043 865 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 3340 4090 5765 882
Size: 617 801 1040 750 1675 2219
Fragments arranged by size:
2219 1675 1040 801 750 617
BsaAI yAC'GTr
Cuts at: 493 1204 4893 493
Size: 711 3689 2702
Fragments arranged by size:
3689 2702 711
BsaBI GATnn'nnATC
Cuts at: 7096 7096
Size: 7102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2541 2723 3132 4909
Size: 53 83 186 936 1008 182 409 1777
Cuts at: 4909 5291 5926 275
Size: 382 635 1451
Fragments arranged by size:
1777 1451 1008 936 635 409 382 186
182 83 53
BsaWI w'CCGG_w
Cuts at: 2318 3435 5535 5933 6207 6354 6765 2318
Size: 1117 2100 398 274 147 411 2655
Fragments arranged by size:
2655 2100 1117 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3065 3095 3578 3608 4189
Size: 30 165 30 1662 30 483 30 581
Cuts at: 4189 4219 1178
Size: 30 4061
Fragments arranged by size:
4061 1662 581 483 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2363 2747 3116 3637 3996 1186
Size: 1012 165 384 369 521 359 4292
Fragments arranged by size:
4292 1012 521 384 369 359 165
BseYI C'CCAG_C
Cuts at: 2598 2630 2776 3761 4169 6305 6801 2598
Size: 32 146 985 408 2136 496 2899
Fragments arranged by size:
2899 2136 985 496 408 146 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6529
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2083 4552 5313 6341 1540
Size: 275 186 82 2469 761 1028 2301
Fragments arranged by size:
2469 2301 1028 761 275 186 82
BsiHKAI G_wGCw'C
Cuts at: 3220 3917 4067 4217 5163 5248 6319 3220
Size: 697 150 150 946 85 1071 4003
Fragments arranged by size:
4003 1071 946 697 150 150 85
BsmI GAATG_Cn'
Cuts at: 4613 4706 4613
Size: 93 7009
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2169 2210 2318 2366 2373 2423
Size: 399 363 16 41 108 48 7 50
Cuts at: 2423 2495 2700 2765 2783 2813 3220 3452 3524
Size: 72 205 65 18 30 407 232 72
Cuts at: 3524 3917 3933 4067 4217 4421 5163 5248 6319
Size: 393 16 134 150 204 742 85 1071
Cuts at: 6319 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 407 399 393 363 232
205 204 150 134 108 85 72 72
65 50 48 41 30 18 16 16
7
BspEI T'CCGG_A
Cuts at: 6765 6765
Size: 7102
BspHI T'CATG_A
Cuts at: 4991 6721 4991
Size: 1730 5372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 2874 2973 3333 3615 4457 844
Size: 1682 348 99 360 282 842 3489
Fragments arranged by size:
3489 1682 842 360 348 282 99
BsrBI CCG'CTC
Cuts at: 1394 1823 2388 2591 4989 1394
Size: 429 565 203 2398 3507
Fragments arranged by size:
3507 2398 565 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 5597 5771 65
Size: 1183 2606 1743 174 1396
Fragments arranged by size:
2606 1743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 3338 5746 5933 1700
Size: 110 297 1231 2408 187 2869
Fragments arranged by size:
2869 2408 1231 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 3036 96
Size: 2940 4162
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7102
BssSI C'ACGA_G
Cuts at: 3478 5162 6174 3478
Size: 1684 1012 4406
Fragments arranged by size:
4406 1684 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7102
BstBI TT'CG_AA
Cuts at: 3153 5906 3153
Size: 2753 4349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7102
BstXI CCAn_nnnn'nTGG
Cuts at: 2574 2574
Size: 7102
BstYI r'GATC_y
Cuts at: 1972 3721 4112 4432 4799 5184 5201 6653 6739
Size: 1749 391 320 367 385 17 1452 86
Cuts at: 6739 6751 7097 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1749 1452 391 385 367 346 320
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4155 4155
Size: 7102
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2310 2400 2436 2478 2767
Size: 552 939 66 240 90 36 42 289
Cuts at: 2767 4345 513
Size: 1578 3270
Fragments arranged by size:
3270 1578 939 552 289 240 90 66
42 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6363
Fragments arranged by size:
6363 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 3772 4618 5412 5432 2425
Size: 1347 846 794 20 4095
Fragments arranged by size:
4095 1347 846 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7102
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7067
DraI TTT'AAA
Cuts at: 4406 4406
Size: 7102
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6109 809
Size: 507 4793 1802
Fragments arranged by size:
4793 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2113 2553
Size: 54 1425 50 275 18 250 33 440
Cuts at: 2553 2748 3990 4044 4128 4549 5438 7067 8
Size: 195 1242 54 84 421 889 1629 43
Fragments arranged by size:
1629 1425 1242 889 440 421 275 250
195 84 54 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2080 4549 1537
Size: 275 268 2469 4090
Fragments arranged by size:
4090 2469 275 268
EarI CTCTTCn'nnn_
Cuts at: 1353 3668 3827 5032 1353
Size: 2315 159 1205 3423
Fragments arranged by size:
3423 2315 1205 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2452 2753 5689 6063 6209 387
Size: 1064 138 863 301 2936 374 146 1280
Fragments arranged by size:
2936 1280 1064 863 374 301 146 138
EcoICRI GAG'CTC
Cuts at: 3915 4065 3915
Size: 150 6952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2876 4126 5165 5762 6549 6817
Size: 393 725 519 1250 1039 597 787 268
Cuts at: 6817 1239
Size: 1524
Fragments arranged by size:
1524 1250 1039 787 725 597 519 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3456 3546 3456
Size: 90 7012
EcoO109I rG'GnC_Cy
Cuts at: 2096 2119 2149 2150 2206 2207 2235 2333 2419
Size: 23 30 1 56 1 28 98 86
Cuts at: 2419 2420 2515 2743 2762 2898 2096
Size: 1 95 228 19 136 6300
Fragments arranged by size:
6300 228 136 98 95 86 56 30
28 23 19 1 1 1
EcoRI G'AATT_C
Cuts at: 4411 4789 4411
Size: 378 6724
FspI TGC'GCA
Cuts at: 2415 5608 2415
Size: 3193 3909
HaeII r_GCGC'y
Cuts at: 1678 2726 2822 3135 6249 1678
Size: 1048 96 313 3114 2531
Fragments arranged by size:
3114 2531 1048 313 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 2660 2692 3800
Size: 32 135 32 652 32 404 32 1108
Cuts at: 3800 3802 3832 3834 3965 3997 4070 4102 4261
Size: 2 30 2 131 32 73 32 159
Cuts at: 4261 4293 5743 5775 5817 5849 1373
Size: 32 1450 32 42 32 2626
Fragments arranged by size:
2626 1450 1108 652 404 159 135 131
73 42 32 32 32 32 32 32
32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4446 4692 669
Size: 988 281 2508 246 3079
Fragments arranged by size:
3079 2508 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7102
HpaI GTT'AAC
Cuts at: 4692 4692
Size: 7102
KasI G'GCGC_C
Cuts at: 2722 3131 2722
Size: 409 6693
KpnI G_GTAC'C
Cuts at: 4427 4427
Size: 7102
MfeI C'AATT_G
Cuts at: 4701 4701
Size: 7102
MluI A'CGCG_T
Cuts at: 6978 6978
Size: 7102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2703 6216 6400 1305
Size: 1398 3513 184 2007
Fragments arranged by size:
3513 2007 1398 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2115 2555 3992 4130 10
Size: 54 1425 343 283 440 1437 138 2982
Fragments arranged by size:
2982 1437 1425 440 343 283 138 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2655 3032 3392 3429 5060 5419 5578
Size: 648 1489 377 360 37 1631 359 159
Cuts at: 5578 518
Size: 2042
Fragments arranged by size:
2042 1631 1489 648 377 360 359 159
37
MspA1I CmG'CkG
Cuts at: 1606 2072 2312 2402 2438 2480 2769 2854 4518
Size: 466 240 90 36 42 289 85 1664
Cuts at: 4518 5195 6343 6588 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1664 1148 677 466 289 245 240
90 85 42 36
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 7102
NarI GG'CG_CC
Cuts at: 2723 3132 2723
Size: 409 6693
NcoI C'CATG_G
Cuts at: 513 1065 2004 4345 513
Size: 552 939 2341 3270
Fragments arranged by size:
3270 2341 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7102
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 7102
NheI G'CTAG_C
Cuts at: 1052 2689 1052
Size: 1637 5465
NotI GC'GGCC_GC
Cuts at: 4549 4549
Size: 7102
NspI r_CATG'y
Cuts at: 1132 1229 3397 4460 6005 1132
Size: 97 2168 1063 1545 2229
Fragments arranged by size:
2229 2168 1545 1063 97
PasI CC'CwG_GG
Cuts at: 1348 2758 2914 1348
Size: 1410 156 5536
Fragments arranged by size:
5536 1410 156
PciI A'CATG_T
Cuts at: 6001 6001
Size: 7102
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7102
PmeI GTTT'AAAC
Cuts at: 4406 4406
Size: 7102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3065 3097 5171 5203 6684 6716 3065
Size: 32 2074 32 1481 32 3451
Fragments arranged by size:
3451 2074 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2515 2119
Size: 396 6706
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7102
PsiI TTA'TAA
Cuts at: 4672 4672
Size: 7102
PspOMI G'GGCC_C
Cuts at: 2149 2165 2206 2362 2419 2761 2149
Size: 16 41 156 57 342 6490
Fragments arranged by size:
6490 342 156 57 41 16
PstI C_TGCA'G
Cuts at: 830 2970 3287 3612 4454 830
Size: 2140 317 325 842 3478
Fragments arranged by size:
3478 2140 842 325 317
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7102
PvuII CAG'CTG
Cuts at: 2854 2854
Size: 7102
RsrII CG'GwC_CG
Cuts at: 2676 2676
Size: 7102
SacI G_AGCT'C
Cuts at: 3917 4067 3917
Size: 150 6952
SacII CC_GC'GG
Cuts at: 2073 2313 2403 2439 2481 2770 2073
Size: 240 90 36 42 289 6405
Fragments arranged by size:
6405 289 240 90 42 36
SalI G'TCGA_C
Cuts at: 1936 4444 1936
Size: 2508 4594
SbfI CC_TGCA'GG
Cuts at: 3612 4454 3612
Size: 842 6260
ScaI AGT'ACT
Cuts at: 3844 5350 7062 3844
Size: 1506 1712 3884
Fragments arranged by size:
3884 1712 1506
SfcI C'TryA_G
Cuts at: 826 1045 2042 2966 3283 3608 4450 5585 6266
Size: 219 997 924 317 325 842 1135 681
Cuts at: 6266 6457 826
Size: 191 1471
Fragments arranged by size:
1471 1135 997 924 842 681 325 317
219 191
SfoI GGC'GCC
Cuts at: 2724 3133 2724
Size: 409 6693
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7102
SgrAI Cr'CCGG_yG
Cuts at: 1700 3338 1700
Size: 1638 5464
SmaI CCC'GGG
Cuts at: 1872 2147 2170 2367 2655 4429 1872
Size: 275 23 197 288 1774 4545
Fragments arranged by size:
4545 1774 288 275 197 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2954 3044 3406 4087 5206 6107
Size: 197 926 1011 90 362 681 1119 901
Cuts at: 6107 6369 820
Size: 262 1553
Fragments arranged by size:
1553 1119 1011 926 901 681 362 262
197 90
SnaBI TAC'GTA
Cuts at: 493 4893 493
Size: 4400 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7102
SphI G_CATG'C
Cuts at: 1132 4460 1132
Size: 3328 3774
SrfI GCCC'GGGC
Cuts at: 2170 2170
Size: 7102
SspI AAT'ATT
Cuts at: 5 52 5026 5
Size: 47 4974 2081
Fragments arranged by size:
4974 2081 47
StuI AGG'CCT
Cuts at: 1517 3166 1517
Size: 1649 5453
StyI C'CwwG_G
Cuts at: 513 1065 2004 2355 4345 4541 513
Size: 552 939 351 1990 196 3074
Fragments arranged by size:
3074 1990 939 552 351 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 2906 3775 4183 5129 5146 5299
Size: 1255 702 624 869 408 946 17 153
Cuts at: 5299 5484 5929 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 946 869 702 624 445 408
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3036 3842 5348
Size: 274 80 33 51 1443 1059 806 1506
Cuts at: 5348 7060 96
Size: 1712 138
Fragments arranged by size:
1712 1506 1443 1059 806 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2417 2449 7062 7094 2417
Size: 32 4613 32 2425
Fragments arranged by size:
4613 2425 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7102
XbaI T'CTAG_A
Cuts at: 4438 4438
Size: 7102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2571 2571
Size: 7102
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7102
XmaI C'CCGG_G
Cuts at: 1870 2145 2168 2365 2653 4427 1870
Size: 275 23 197 288 1774 4545
Fragments arranged by size:
4545 1774 288 275 197 23
XmnI GAAnn'nnTTC
Cuts at: 3080 3473 5231 3080
Size: 393 1758 4951
Fragments arranged by size:
4951 1758 393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4910 276
Size: 53 83 186 936 3376 2468
Fragments arranged by size:
3376 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PspXI PsrI SanDI SapI
SexAI SfiI SwaI